JPS5661996A - Preparation of polysaccharide - Google Patents
Preparation of polysaccharideInfo
- Publication number
- JPS5661996A JPS5661996A JP13529179A JP13529179A JPS5661996A JP S5661996 A JPS5661996 A JP S5661996A JP 13529179 A JP13529179 A JP 13529179A JP 13529179 A JP13529179 A JP 13529179A JP S5661996 A JPS5661996 A JP S5661996A
- Authority
- JP
- Japan
- Prior art keywords
- polysaccharide
- give
- water
- centrifuged
- precipitate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
PURPOSE: To prepare a polysaccharide in high yield, by inoculating a bacterial medium consisting of methanol as a main carbon source with a specific bacterium belonging to the genus Pseudomonas so that the polysaccharide is accumulated, followed by collecting it from the medium.
CONSTITUTION: Pseudomonas sp. ID-196, a bacterium belonging to the genus Pseudomonas is inoculated on a bacterial medium consisting of methanol as a main carbon source to accumulate a polysaccaride in high yield. The culture solution is diluted by 10W20 times and filtered or centrifuged to remove solid bodies or materials, and twice as much acetone as the solution by weight is added to it to give a crude polysaccharide. The polysaccharide is dissolved in water again and subjected to the operation of protein removal, e.g., heat treatment, precipitation of isoelectric point, etc., and centrifuged to give a supernatant liquid, to which acetone is added to deposit a precipitate. The precipitate is dissolved in water, desalted by a ratio of water dialysis to give a white spongy purified polysaccharide.
COPYRIGHT: (C)1981,JPO&Japio
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP13529179A JPS5661996A (en) | 1979-10-22 | 1979-10-22 | Preparation of polysaccharide |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP13529179A JPS5661996A (en) | 1979-10-22 | 1979-10-22 | Preparation of polysaccharide |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPS5661996A true JPS5661996A (en) | 1981-05-27 |
Family
ID=15148258
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP13529179A Pending JPS5661996A (en) | 1979-10-22 | 1979-10-22 | Preparation of polysaccharide |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS5661996A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS63240778A (en) * | 1987-03-30 | 1988-10-06 | Res Dev Corp Of Japan | Culture method for microorganism |
| CN104155250A (en) * | 2014-08-15 | 2014-11-19 | 广州衡创测试技术服务有限公司 | Crude polysaccharide determination method |
-
1979
- 1979-10-22 JP JP13529179A patent/JPS5661996A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS63240778A (en) * | 1987-03-30 | 1988-10-06 | Res Dev Corp Of Japan | Culture method for microorganism |
| CN104155250A (en) * | 2014-08-15 | 2014-11-19 | 广州衡创测试技术服务有限公司 | Crude polysaccharide determination method |
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