JPS5668691A - Method for concentration and purification of human fibroplastic interferon - Google Patents
Method for concentration and purification of human fibroplastic interferonInfo
- Publication number
- JPS5668691A JPS5668691A JP14324779A JP14324779A JPS5668691A JP S5668691 A JPS5668691 A JP S5668691A JP 14324779 A JP14324779 A JP 14324779A JP 14324779 A JP14324779 A JP 14324779A JP S5668691 A JPS5668691 A JP S5668691A
- Authority
- JP
- Japan
- Prior art keywords
- interferon
- solution
- fibroplastic
- contact
- carrier
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 102000014150 Interferons Human genes 0.000 title abstract 7
- 108010050904 Interferons Proteins 0.000 title abstract 7
- 229940079322 interferon Drugs 0.000 title abstract 7
- 230000002344 fibroplastic effect Effects 0.000 title abstract 3
- 238000000034 method Methods 0.000 title abstract 3
- 238000000746 purification Methods 0.000 title 1
- PXHVJJICTQNCMI-UHFFFAOYSA-N Nickel Chemical compound [Ni] PXHVJJICTQNCMI-UHFFFAOYSA-N 0.000 abstract 4
- 230000002378 acidificating effect Effects 0.000 abstract 3
- 150000001768 cations Chemical class 0.000 abstract 2
- 239000013522 chelant Substances 0.000 abstract 2
- 239000003480 eluent Substances 0.000 abstract 2
- 229910052759 nickel Inorganic materials 0.000 abstract 2
- 229920005654 Sephadex Polymers 0.000 abstract 1
- 239000012507 Sephadex™ Substances 0.000 abstract 1
- 239000003463 adsorbent Substances 0.000 abstract 1
- 230000000259 anti-tumor effect Effects 0.000 abstract 1
- 230000000840 anti-viral effect Effects 0.000 abstract 1
- 239000000969 carrier Substances 0.000 abstract 1
- 239000012141 concentrate Substances 0.000 abstract 1
- 230000000694 effects Effects 0.000 abstract 1
- 239000000126 substance Substances 0.000 abstract 1
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Peptides Or Proteins (AREA)
Abstract
PURPOSE: To concentrate and purify the titled substance having an antiviral and antitumor activity to a high concentration, by treating a solution of a crude human fibroplastic interferon into contact with a strongly acidic cation exchanger and then a nickel chelate carrier.
CONSTITUTION: A solution of a crude human fibroplastic interferon is brought into contact with a strongly acidic cation exchanger, e.g. SP-Sephadex C-25 column, under acidic conditions, to adsorb the interferon on the adsorbent. An eluent is used to recover the interferon as a solution. The recovered solution is then brought into contact with a carrier bonded to a chelate group in which nickel is chelated to adsrob the interferon on the carrier. The adsorbed interferon is recovered by an eluent as a solution. According to the method, the concentrating and purifying effect can be more remarkably improved than the method in which the carriers are used alone.
COPYRIGHT: (C)1981,JPO&Japio
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP14324779A JPS5668691A (en) | 1979-11-07 | 1979-11-07 | Method for concentration and purification of human fibroplastic interferon |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP14324779A JPS5668691A (en) | 1979-11-07 | 1979-11-07 | Method for concentration and purification of human fibroplastic interferon |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPS5668691A true JPS5668691A (en) | 1981-06-09 |
Family
ID=15334301
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP14324779A Pending JPS5668691A (en) | 1979-11-07 | 1979-11-07 | Method for concentration and purification of human fibroplastic interferon |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS5668691A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS59167597A (en) * | 1983-03-03 | 1984-09-21 | エフ・ホフマン・ラ・ロシユ・ウント・コンパニ−・アクチエンゲゼルシヤフト | Purification of recombined interferon |
-
1979
- 1979-11-07 JP JP14324779A patent/JPS5668691A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS59167597A (en) * | 1983-03-03 | 1984-09-21 | エフ・ホフマン・ラ・ロシユ・ウント・コンパニ−・アクチエンゲゼルシヤフト | Purification of recombined interferon |
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