JPS5673342A - Enzyme electrode and its manufacture - Google Patents
Enzyme electrode and its manufactureInfo
- Publication number
- JPS5673342A JPS5673342A JP14952879A JP14952879A JPS5673342A JP S5673342 A JPS5673342 A JP S5673342A JP 14952879 A JP14952879 A JP 14952879A JP 14952879 A JP14952879 A JP 14952879A JP S5673342 A JPS5673342 A JP S5673342A
- Authority
- JP
- Japan
- Prior art keywords
- enzyme
- layer
- film
- electrode
- bonding layer
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 102000004190 Enzymes Human genes 0.000 title abstract 9
- 108090000790 Enzymes Proteins 0.000 title abstract 6
- 238000004519 manufacturing process Methods 0.000 title 1
- 230000035515 penetration Effects 0.000 abstract 3
- 239000002131 composite material Substances 0.000 abstract 2
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 abstract 2
- 239000000758 substrate Substances 0.000 abstract 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 abstract 1
- LEHOTFFKMJEONL-UHFFFAOYSA-N Uric Acid Chemical compound N1C(=O)NC(=O)C2=C1NC(=O)N2 LEHOTFFKMJEONL-UHFFFAOYSA-N 0.000 abstract 1
- TVWHNULVHGKJHS-UHFFFAOYSA-N Uric acid Natural products N1C(=O)NC(=O)C2NC(=O)NC21 TVWHNULVHGKJHS-UHFFFAOYSA-N 0.000 abstract 1
- 239000011521 glass Substances 0.000 abstract 1
- 239000008103 glucose Substances 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 238000012856 packing Methods 0.000 abstract 1
- 229910052697 platinum Inorganic materials 0.000 abstract 1
- 239000000126 substance Substances 0.000 abstract 1
- 229940116269 uric acid Drugs 0.000 abstract 1
Landscapes
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
PURPOSE: To obtain an enzyme electrode capable of superior response speed and operating rate by incorporating a selective penetration layer and an enzyme bonding layer together and attaching an enzyme bonding layer in the state of facing to the sensitive part of an electrode.
CONSTITUTION: A selective penetration layer is formed on a substrate of glass, etc., and on the layer, a porous layer is formed. In this porous layer, enzymes such as glucose oxytase, etc. is fixed under a chemical bonding process to form anenzyme bonding layer. Then a composite enzyme film being formed is stripped off the substrate. This composite enzyme film 8 is attached to a body 4 with the use of a cover 6 through packings 5, 9 in such manner that said film be applied to an electrode of platinum pole 1, silver/chlorinated silver pole 2, etc. through an enzyme- penetrable film 7 with the enzyme bonding layer 8a facing inwards and the selective penetration layer 8b facing outwards. This enzyme electrode can be used for uric acid analysis in a stable condition for a long time.
COPYRIGHT: (C)1981,JPO&Japio
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP14952879A JPS5673342A (en) | 1979-11-20 | 1979-11-20 | Enzyme electrode and its manufacture |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP14952879A JPS5673342A (en) | 1979-11-20 | 1979-11-20 | Enzyme electrode and its manufacture |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPS5673342A true JPS5673342A (en) | 1981-06-18 |
Family
ID=15477100
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP14952879A Pending JPS5673342A (en) | 1979-11-20 | 1979-11-20 | Enzyme electrode and its manufacture |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS5673342A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS61114156A (en) * | 1984-11-08 | 1986-05-31 | Nok Corp | Fixing method of vinyl chloride resin film onto silicon nitride surface |
| JPS625169A (en) * | 1985-06-28 | 1987-01-12 | マイルズ ラボラトリ−ス インコ−ポレ−テッド | Electrochemical sensor, film for said sensor and manufacture of said film |
| JPS62254051A (en) * | 1986-04-17 | 1987-11-05 | Daikin Ind Ltd | Enzyme electrode |
| JPS6332478A (en) * | 1986-07-28 | 1988-02-12 | Natl Food Res Inst | Enzymic reaction apparatus |
| US6280587B1 (en) | 1998-07-02 | 2001-08-28 | Nec Corporation | Enzyme electrode and a biosensor and a measuring apparatus therewith |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CH569973A5 (en) * | 1972-09-08 | 1975-11-28 | Radiometer As | |
| JPS52139777A (en) * | 1976-05-14 | 1977-11-21 | Omron Tateisi Electronics Co | Fixed enzyme membrane |
| JPS53140092A (en) * | 1977-05-13 | 1978-12-06 | Omron Tateisi Electronics Co | Enzyme electrode |
| JPS54102193A (en) * | 1978-01-28 | 1979-08-11 | Toyo Boseki | Enzyme film for oxygen electrode |
-
1979
- 1979-11-20 JP JP14952879A patent/JPS5673342A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CH569973A5 (en) * | 1972-09-08 | 1975-11-28 | Radiometer As | |
| JPS52139777A (en) * | 1976-05-14 | 1977-11-21 | Omron Tateisi Electronics Co | Fixed enzyme membrane |
| JPS53140092A (en) * | 1977-05-13 | 1978-12-06 | Omron Tateisi Electronics Co | Enzyme electrode |
| JPS54102193A (en) * | 1978-01-28 | 1979-08-11 | Toyo Boseki | Enzyme film for oxygen electrode |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS61114156A (en) * | 1984-11-08 | 1986-05-31 | Nok Corp | Fixing method of vinyl chloride resin film onto silicon nitride surface |
| JPS625169A (en) * | 1985-06-28 | 1987-01-12 | マイルズ ラボラトリ−ス インコ−ポレ−テッド | Electrochemical sensor, film for said sensor and manufacture of said film |
| JPS62254051A (en) * | 1986-04-17 | 1987-11-05 | Daikin Ind Ltd | Enzyme electrode |
| JPS6332478A (en) * | 1986-07-28 | 1988-02-12 | Natl Food Res Inst | Enzymic reaction apparatus |
| US6280587B1 (en) | 1998-07-02 | 2001-08-28 | Nec Corporation | Enzyme electrode and a biosensor and a measuring apparatus therewith |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US4356074A (en) | Substrate specific galactose oxidase enzyme electrodes | |
| US4073713A (en) | Membrane for enzyme electrodes | |
| Koryta | Theory and applications of ion-selective electrodes part II | |
| Palleschi et al. | Amperometric tetrathiafulvalene-mediated lactate electrode using lactate oxidase absorbed on carbon foil | |
| JPS5441191A (en) | Glucose-oxygen sensitive electrode | |
| JPS63131057A (en) | Enzyme sensor | |
| JPS5510583A (en) | Enzyme electrode and its manufacture | |
| JPS56163447A (en) | Enzyme electrode | |
| JPH0777511A (en) | Biosensor for measuring gas and manufacturing method thereof | |
| JPS5770448A (en) | Enzyme electrode | |
| KR870008189A (en) | Enzyme sensor and manufacturing method | |
| IL78560A0 (en) | Electrochemical sensor and membrane therefor | |
| Kano et al. | Enzyme microelectrodes for choline and acetylcholine and their applications | |
| EP0216467A3 (en) | Two-dimensional diffusion glucose substrate sensing electrode | |
| JPS57177699A (en) | Determination of alpha-amylase by means of enzyme electrode method | |
| Genshaw et al. | Whole blood glucose enzyme electrode | |
| JPS585642A (en) | Enzyme electrode | |
| JPS5623785A (en) | Solar battery | |
| JPS5244692A (en) | Ion activity measuring electrode | |
| JPS5598347A (en) | Enzyme electrode | |
| JPS57122797A (en) | Production of immobilized enzyme membrane | |
| GB2230606A (en) | Bioelectrochemical reactions in organic solvents | |
| RU93008860A (en) | ELECTROCHEMICAL KNOT OF AMEROMETRIC BIODATCHIK AND METHOD OF ITS MANUFACTURE | |
| JPS5578241A (en) | Enzyme electrode | |
| JPS5217889A (en) | Enzyme electrode |