JPS57108018A - Endotoxin-sensitive substance - Google Patents
Endotoxin-sensitive substanceInfo
- Publication number
- JPS57108018A JPS57108018A JP55096176A JP9617680A JPS57108018A JP S57108018 A JPS57108018 A JP S57108018A JP 55096176 A JP55096176 A JP 55096176A JP 9617680 A JP9617680 A JP 9617680A JP S57108018 A JPS57108018 A JP S57108018A
- Authority
- JP
- Japan
- Prior art keywords
- endotoxin
- sensitive substance
- amebosite
- horseshoe crab
- substance
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000002158 endotoxin Substances 0.000 title abstract 7
- 239000000126 substance Substances 0.000 title abstract 5
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 abstract 4
- 241001529572 Chaceon affinis Species 0.000 abstract 3
- 238000001042 affinity chromatography Methods 0.000 abstract 2
- 239000007853 buffer solution Substances 0.000 abstract 2
- 108010055222 clotting enzyme Proteins 0.000 abstract 2
- 108090000623 proteins and genes Proteins 0.000 abstract 2
- 102000004169 proteins and genes Human genes 0.000 abstract 2
- 239000011780 sodium chloride Substances 0.000 abstract 2
- 239000000758 substrate Substances 0.000 abstract 2
- 229920000936 Agarose Polymers 0.000 abstract 1
- 241000894006 Bacteria Species 0.000 abstract 1
- 108090000790 Enzymes Proteins 0.000 abstract 1
- 102000004190 Enzymes Human genes 0.000 abstract 1
- 108010074860 Factor Xa Proteins 0.000 abstract 1
- 108090000190 Thrombin Proteins 0.000 abstract 1
- 238000010521 absorption reaction Methods 0.000 abstract 1
- 239000008280 blood Substances 0.000 abstract 1
- 210000004369 blood Anatomy 0.000 abstract 1
- 238000001514 detection method Methods 0.000 abstract 1
- 229940088598 enzyme Drugs 0.000 abstract 1
- 239000000463 material Substances 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 229960004072 thrombin Drugs 0.000 abstract 1
Landscapes
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
NEW MATERIAL:An endotoxin-sensitive substance. A protein having an ultraviolet absorption at 280nm and having the property to hydrolyze the thrombin and the enzyme substrate of the known clotting enzyme of horseshoe crab by the action of endotoxin originated from Gram-negative bacteria.
USE: Substance for the detection and determination of endotoxin.
PROCESS: Amebosite obtained from the limph of horseshoe crab blood is extracted with hypotonic extractant to obtain amebosite.lizate, which is subjected to the affinity chromatography using a carrier such as heparin-bonded agarose derivative and eluted with a buffer solution containing 0.154MW0.5M NaCl to obtain an endotoxin-sensitive substance as a protein fraction. When the endotoxin-sensitive substance and the amebosite.lizate are subjected to the affinity chromatography, and endotoxin is added to the fraction obtained above in combination with the fraction obtained by the buffer solution containing ≤0.154M NaCl, then coagulation factor Xa and the enzume substrate of the known clotting enzyme of horseshoe crab are hydrolyzed.
COPYRIGHT: (C)1982,JPO&Japio
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP55096176A JPS57108018A (en) | 1980-07-16 | 1980-07-16 | Endotoxin-sensitive substance |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP55096176A JPS57108018A (en) | 1980-07-16 | 1980-07-16 | Endotoxin-sensitive substance |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS57108018A true JPS57108018A (en) | 1982-07-05 |
| JPH0323869B2 JPH0323869B2 (en) | 1991-03-29 |
Family
ID=14158010
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP55096176A Granted JPS57108018A (en) | 1980-07-16 | 1980-07-16 | Endotoxin-sensitive substance |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS57108018A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5813516A (en) * | 1981-07-16 | 1983-01-26 | Seikagaku Kogyo Co Ltd | Preparation of amebocyte lysate |
-
1980
- 1980-07-16 JP JP55096176A patent/JPS57108018A/en active Granted
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5813516A (en) * | 1981-07-16 | 1983-01-26 | Seikagaku Kogyo Co Ltd | Preparation of amebocyte lysate |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0323869B2 (en) | 1991-03-29 |
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