JPS5719661A - Group of microcapsules for immune reaction and discrimination method using the same - Google Patents
Group of microcapsules for immune reaction and discrimination method using the sameInfo
- Publication number
- JPS5719661A JPS5719661A JP9340680A JP9340680A JPS5719661A JP S5719661 A JPS5719661 A JP S5719661A JP 9340680 A JP9340680 A JP 9340680A JP 9340680 A JP9340680 A JP 9340680A JP S5719661 A JPS5719661 A JP S5719661A
- Authority
- JP
- Japan
- Prior art keywords
- capsules
- antibodies
- antigens
- testing
- different
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000003094 microcapsule Substances 0.000 title abstract 3
- 238000012850 discrimination method Methods 0.000 title 1
- 230000008105 immune reaction Effects 0.000 title 1
- 239000000427 antigen Substances 0.000 abstract 5
- 102000036639 antigens Human genes 0.000 abstract 5
- 108091007433 antigens Proteins 0.000 abstract 5
- 239000002775 capsule Substances 0.000 abstract 5
- 239000003086 colorant Substances 0.000 abstract 2
- 239000011162 core material Substances 0.000 abstract 2
- 239000000975 dye Substances 0.000 abstract 2
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 abstract 1
- 230000004520 agglutination Effects 0.000 abstract 1
- 125000000524 functional group Chemical group 0.000 abstract 1
- 208000006454 hepatitis Diseases 0.000 abstract 1
- 231100000283 hepatitis Toxicity 0.000 abstract 1
- 206010022000 influenza Diseases 0.000 abstract 1
- 239000000463 material Substances 0.000 abstract 1
- 239000000126 substance Substances 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
- G01N33/532—Production of labelled immunochemicals
Landscapes
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Medicinal Preparation (AREA)
Abstract
PURPOSE:To discriminate a plurality of antigens (antibodies) with only one testing, by incorporating different dyes into a core material of a microcapsule, combining different antigens (antibodies) with the outer walls of respective capsules separately and mixing these capsules for use. CONSTITUTION:By incorporating dyes and fluorescent dyestuffs having different colors into a biochemically inactive substance, such as oil or fat, as a core material, a microcapsule having a functional group such as NH2 or OH in its wall material is prepared. To the respective capsules antigens (antibodies) are combined separately through glutaraldehyde, etc., to obtain sensitized capsules. By adding a specimen in a test tube containing at the same time a plurality of different, capsules to react with an antibody (antigen) in said specimen and by measuring chromatic light emitted from the agglutination products using filters in responce to the respective colors, a plurality of antigens (antibodies) are discriminated with one testing. Thus it is possible to identify e.g., a type of influenza, hepatitis, etc., with one testing.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP9340680A JPS6058420B2 (en) | 1980-07-09 | 1980-07-09 | Microcapsule group for immune reaction and discrimination method using the same |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP9340680A JPS6058420B2 (en) | 1980-07-09 | 1980-07-09 | Microcapsule group for immune reaction and discrimination method using the same |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS5719661A true JPS5719661A (en) | 1982-02-01 |
| JPS6058420B2 JPS6058420B2 (en) | 1985-12-19 |
Family
ID=14081411
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP9340680A Expired JPS6058420B2 (en) | 1980-07-09 | 1980-07-09 | Microcapsule group for immune reaction and discrimination method using the same |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS6058420B2 (en) |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5984162A (en) * | 1982-11-05 | 1984-05-15 | Toshiba Corp | Immunological analysis method |
| JPS6035265A (en) * | 1983-05-19 | 1985-02-23 | イオアニス・トリパトツイス | Particle and method of detecting antigen and/or antibody by using particle |
| JPS60224068A (en) * | 1984-04-20 | 1985-11-08 | Sekisui Chem Co Ltd | Method for deciding pathogenic bacterium in opportunistic infection |
| JPS60242370A (en) * | 1984-05-17 | 1985-12-02 | Imunisu:Kk | Colored particle for immunodiagnosis |
| JPS6176958A (en) * | 1984-09-06 | 1986-04-19 | ザ ウエルカム フアウンデーシヨン リミテツド | Diagnostic testing method and kit used for said method |
| JPS6182165A (en) * | 1984-06-15 | 1986-04-25 | マスト イミユノシステムズ インコ−ポレ−テツド | Damping agent inhibiting external beam in luminescent specific connection test |
| US4752572A (en) * | 1985-08-30 | 1988-06-21 | Eastman Kodak Company | Lipid vesicles containing labeled species and their analytical uses |
| JPS63255660A (en) * | 1987-04-11 | 1988-10-21 | Hitachi Ltd | Cell measurement method |
-
1980
- 1980-07-09 JP JP9340680A patent/JPS6058420B2/en not_active Expired
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5984162A (en) * | 1982-11-05 | 1984-05-15 | Toshiba Corp | Immunological analysis method |
| JPS6035265A (en) * | 1983-05-19 | 1985-02-23 | イオアニス・トリパトツイス | Particle and method of detecting antigen and/or antibody by using particle |
| JPS60224068A (en) * | 1984-04-20 | 1985-11-08 | Sekisui Chem Co Ltd | Method for deciding pathogenic bacterium in opportunistic infection |
| JPS60242370A (en) * | 1984-05-17 | 1985-12-02 | Imunisu:Kk | Colored particle for immunodiagnosis |
| JPS6182165A (en) * | 1984-06-15 | 1986-04-25 | マスト イミユノシステムズ インコ−ポレ−テツド | Damping agent inhibiting external beam in luminescent specific connection test |
| JPS6176958A (en) * | 1984-09-06 | 1986-04-19 | ザ ウエルカム フアウンデーシヨン リミテツド | Diagnostic testing method and kit used for said method |
| US4752572A (en) * | 1985-08-30 | 1988-06-21 | Eastman Kodak Company | Lipid vesicles containing labeled species and their analytical uses |
| JPS63255660A (en) * | 1987-04-11 | 1988-10-21 | Hitachi Ltd | Cell measurement method |
Also Published As
| Publication number | Publication date |
|---|---|
| JPS6058420B2 (en) | 1985-12-19 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US4115535A (en) | Diagnostic method employing a mixture of normally separable protein-coated particles | |
| FI96639C (en) | Standalone immunoassay | |
| ES2079390T3 (en) | REAGENTS, METHOD AND KITS FOR AN IMMUNOLOGICAL TEST OF AMPHETAMINE FLUORESCENCE POLARIZATION. | |
| US20090253218A1 (en) | Method for serologic agglutination and other immunoassays performed in a thin film fluid sample | |
| US4483928A (en) | Microcapsules sensitized with antibody and a method for measurement of lymphocyte using the same based on cell-mediated immunity | |
| US4407964A (en) | Homogeneous fluoroimmunoassay involving sensing radiation for forward and back directions | |
| US4130395A (en) | Process and apparatus for detection of specific biological factors by means of osmotic hemolysis | |
| JPH04506113A (en) | Methods, reagents and test kits for quantifying subpopulations of organisms | |
| ATE37095T1 (en) | FLUORESCENCE DETERMINATION OF BLOOD TYPES. | |
| JPS5745454A (en) | Immunochemical measuring method for various minor components | |
| Murdoch et al. | Alkaline phosphatase-fast red, a new fluorescent label: application in double labelling for cell surface antigen and cell cycle analysis | |
| EP0222341B1 (en) | A method for immunoassay and reagents therefor | |
| GB1506017A (en) | Fluorometric system and method for the detection of biologically derived substances | |
| JPS5719661A (en) | Group of microcapsules for immune reaction and discrimination method using the same | |
| Seefeldt et al. | Comparison of enzyme-linked immunosorbent assay, indirect fluorescent antibody test, and direct agglutination test for detecting Toxoplasma gondii antibodies in naturally aborted ovine fetuses | |
| SE7704305L (en) | PROCEDURE FOR ANALYSIS OF A BIOLOGICAL LIQUID TEST | |
| SE7706412L (en) | METHODS AND APPARATUS FOR SIGNALING THE INFORMATION OF CHEMICAL REACTION COMPONENTS IN A CHEMICAL ANALYSIS SYSTEM | |
| US4758523A (en) | Immunoassay method for removing excess tracer | |
| JPH0750113B2 (en) | Laser magnetic immunoassay | |
| US8835121B2 (en) | Modified method of agglutination to detect infections caused by microorganisms | |
| EP0241042A2 (en) | A method for cell analysis | |
| CA2097952A1 (en) | Early diagnosis of sepsis utilizing antigen-antibody interactions amplified by whole blood chemiluminescence | |
| Wang et al. | Antibody detection: Principles and applications | |
| ATE135820T1 (en) | IMMUNOASSAY METHODS AND LIQUID REAGENTS THEREFOR | |
| WO1987001810A1 (en) | Method for carrying out immunoassays by using enzyme labels |