JPS571970A - Measuring method for agglutination for diagonostic latex reagent - Google Patents

Measuring method for agglutination for diagonostic latex reagent

Info

Publication number
JPS571970A
JPS571970A JP7637080A JP7637080A JPS571970A JP S571970 A JPS571970 A JP S571970A JP 7637080 A JP7637080 A JP 7637080A JP 7637080 A JP7637080 A JP 7637080A JP S571970 A JPS571970 A JP S571970A
Authority
JP
Japan
Prior art keywords
antigen
latex
treated
sensitively
agglutination
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP7637080A
Other languages
Japanese (ja)
Other versions
JPS6352705B2 (en
Inventor
Toichi Yamada
Michio Kuge
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sekisui Chemical Co Ltd
Original Assignee
Sekisui Chemical Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sekisui Chemical Co Ltd filed Critical Sekisui Chemical Co Ltd
Priority to JP7637080A priority Critical patent/JPS571970A/en
Publication of JPS571970A publication Critical patent/JPS571970A/en
Publication of JPS6352705B2 publication Critical patent/JPS6352705B2/ja
Granted legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form

Landscapes

  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

PURPOSE:To enable high sensitization through the suppression of nonsingular agglutination, by a method wherein an antigen is treated into a condition to sensitively react to latex particles, and a liquid to be measured, containing the antigen, is caused to react to a latex reagent, in which the latex particles, being so sensitively reacted with the antigen, are treated with a serum containing the antigen, to optically measure a change in turbidity. CONSTITUTION:The latex reagent is prepared such that, after an antigen is treated into a condition to sensitively react to a polystyrene latex and the like, a latex, to which so treated antigen reacts sensitively, is added to an antiserum for the treatment, excess antiserum is removed, and the resultant liquid is resuspended into a buffer liquid. Said treatment enables the effective suppression of nonsingular agglutination. After a phosphatic buffer liquid is added to said latex reagent, a detecting substance, containing an antigen, is added for mixture to immediately record a change with the elapse of time in an absorbance of a light having a given wave length. After starting to record, a light-absorption increasing factor is found at an earliest time from the absorbance which increases rectilineally to measure the antigen. In the case of B type virus surface antigen, this permits the measurement at the sensitivity which is 1,000 times as high as that of conventional types.
JP7637080A 1980-06-05 1980-06-05 Measuring method for agglutination for diagonostic latex reagent Granted JPS571970A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP7637080A JPS571970A (en) 1980-06-05 1980-06-05 Measuring method for agglutination for diagonostic latex reagent

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP7637080A JPS571970A (en) 1980-06-05 1980-06-05 Measuring method for agglutination for diagonostic latex reagent

Publications (2)

Publication Number Publication Date
JPS571970A true JPS571970A (en) 1982-01-07
JPS6352705B2 JPS6352705B2 (en) 1988-10-19

Family

ID=13603453

Family Applications (1)

Application Number Title Priority Date Filing Date
JP7637080A Granted JPS571970A (en) 1980-06-05 1980-06-05 Measuring method for agglutination for diagonostic latex reagent

Country Status (1)

Country Link
JP (1) JPS571970A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS59187264A (en) * 1983-03-30 1984-10-24 Kyowa Hakko Kogyo Co Ltd Measurement of antigen-antibody reaction speed

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5314015A (en) * 1976-07-21 1978-02-08 Dainippon Ink & Chemicals Method of producing painted board with protruding pattern
JPS562552A (en) * 1979-06-14 1981-01-12 Warner Lambert Co Dynamic latex cohesin cohesion measurement method

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5314015A (en) * 1976-07-21 1978-02-08 Dainippon Ink & Chemicals Method of producing painted board with protruding pattern
JPS562552A (en) * 1979-06-14 1981-01-12 Warner Lambert Co Dynamic latex cohesin cohesion measurement method

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS59187264A (en) * 1983-03-30 1984-10-24 Kyowa Hakko Kogyo Co Ltd Measurement of antigen-antibody reaction speed

Also Published As

Publication number Publication date
JPS6352705B2 (en) 1988-10-19

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