JPS572700A - Analyzing method with immobilized enzyme - Google Patents
Analyzing method with immobilized enzymeInfo
- Publication number
- JPS572700A JPS572700A JP7702780A JP7702780A JPS572700A JP S572700 A JPS572700 A JP S572700A JP 7702780 A JP7702780 A JP 7702780A JP 7702780 A JP7702780 A JP 7702780A JP S572700 A JPS572700 A JP S572700A
- Authority
- JP
- Japan
- Prior art keywords
- immobilized enzyme
- acid
- enzyme
- immobilized
- urease
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 108010093096 Immobilized Enzymes Proteins 0.000 title abstract 4
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 abstract 6
- 108010089254 Cholesterol oxidase Proteins 0.000 abstract 2
- 108010015776 Glucose oxidase Proteins 0.000 abstract 2
- 239000004366 Glucose oxidase Substances 0.000 abstract 2
- 102000003992 Peroxidases Human genes 0.000 abstract 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 abstract 2
- 108010046334 Urease Proteins 0.000 abstract 2
- 230000002378 acidificating effect Effects 0.000 abstract 2
- 239000007853 buffer solution Substances 0.000 abstract 2
- 229940116332 glucose oxidase Drugs 0.000 abstract 2
- 235000019420 glucose oxidase Nutrition 0.000 abstract 2
- 108040007629 peroxidase activity proteins Proteins 0.000 abstract 2
- KDYFGRWQOYBRFD-UHFFFAOYSA-N succinic acid Chemical compound OC(=O)CCC(O)=O KDYFGRWQOYBRFD-UHFFFAOYSA-N 0.000 abstract 2
- 108090000790 Enzymes Proteins 0.000 abstract 1
- 102000004190 Enzymes Human genes 0.000 abstract 1
- 239000002253 acid Substances 0.000 abstract 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 abstract 1
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 abstract 1
- 239000004327 boric acid Substances 0.000 abstract 1
- 229940088598 enzyme Drugs 0.000 abstract 1
- 239000001384 succinic acid Substances 0.000 abstract 1
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
PURPOSE: To improve the durability of an immobilized enzyme, by using a boric acid buffer solution containing citric acid in a column packed with the immobilized enzyme having the optimal pH in an acidic region, e.g. glucose oxidase, cholesterol oxidase, peroxidase, urease, etc.
CONSTITUTION: An enzyme, e.g. glucose oxidase, cholesterol oxidase, peroxidase or urease, having the optimal pH in an acidic region is immobilized by the well- known method. The resultant immobilized enzyme is then packed in a column, and the enzymic analysis is carried out with a phosphoric acid buffer solution containing an acid, e.g. citric acid or succinic acid.
COPYRIGHT: (C)1982,JPO&Japio
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP7702780A JPS572700A (en) | 1980-06-06 | 1980-06-06 | Analyzing method with immobilized enzyme |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP7702780A JPS572700A (en) | 1980-06-06 | 1980-06-06 | Analyzing method with immobilized enzyme |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPS572700A true JPS572700A (en) | 1982-01-08 |
Family
ID=13622257
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP7702780A Pending JPS572700A (en) | 1980-06-06 | 1980-06-06 | Analyzing method with immobilized enzyme |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS572700A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS60101792U (en) * | 1983-12-19 | 1985-07-11 | 沖電気工業株式会社 | Unit removal tool |
-
1980
- 1980-06-06 JP JP7702780A patent/JPS572700A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS60101792U (en) * | 1983-12-19 | 1985-07-11 | 沖電気工業株式会社 | Unit removal tool |
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