JPS5772048A - Component analyzing method - Google Patents

Component analyzing method

Info

Publication number
JPS5772048A
JPS5772048A JP14813680A JP14813680A JPS5772048A JP S5772048 A JPS5772048 A JP S5772048A JP 14813680 A JP14813680 A JP 14813680A JP 14813680 A JP14813680 A JP 14813680A JP S5772048 A JPS5772048 A JP S5772048A
Authority
JP
Japan
Prior art keywords
measurement
final light
reference value
aref
measuring
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP14813680A
Other languages
Japanese (ja)
Other versions
JPH0121897B2 (en
Inventor
Sugio Mabe
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Olympus Corp
Original Assignee
Olympus Corp
Olympus Optical Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Olympus Corp, Olympus Optical Co Ltd filed Critical Olympus Corp
Priority to JP14813680A priority Critical patent/JPS5772048A/en
Publication of JPS5772048A publication Critical patent/JPS5772048A/en
Publication of JPH0121897B2 publication Critical patent/JPH0121897B2/ja
Granted legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N21/27—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands using photo-electric detection ; circuits for computing concentration
    • G01N21/272—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands using photo-electric detection ; circuits for computing concentration for following a reaction, e.g. for determining photometrically a reaction rate (photometric cinetic analysis)

Landscapes

  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Mathematical Physics (AREA)
  • Theoretical Computer Science (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

PURPOSE:To perform a reliable and rapid measurement, by a method wherein, in a component analysis by a reaction speed measuring method for a biochemical component, a plurality of light measurements are conducted in a reaction process, and a selection is made through the comparison of a final light measurement value with a preset reference value. CONSTITUTION:In a measurement of an enzyme amount of a biochemical components, e.g., glutamic oxalacetic transminase (GOT) and the like, an absorbancy is measured at several points in a reaction process, and the results are compared with a reference value Aref previously within a range of a final light measuring time previously determined. In the case of a final light measurement value Aend, e.g., 5 in the Figure, exceeding the Aref, a reaction speed at a distance between measuring points 1-4 before a measuring point 5 is computed. In case the absorbancy Aend at the final light measuring point 5 is below the reference value Aref, a reaction speed at a distance between the mesuring points 1-5 is computed. The sufficent utilization of a reagent concentration in this manner enables to perform a highly precise measurement of the GOT so as to prevent the production of an eroneous result due to the deterioration or preparation error of the reagent.
JP14813680A 1980-10-24 1980-10-24 Component analyzing method Granted JPS5772048A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP14813680A JPS5772048A (en) 1980-10-24 1980-10-24 Component analyzing method

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP14813680A JPS5772048A (en) 1980-10-24 1980-10-24 Component analyzing method

Publications (2)

Publication Number Publication Date
JPS5772048A true JPS5772048A (en) 1982-05-06
JPH0121897B2 JPH0121897B2 (en) 1989-04-24

Family

ID=15446067

Family Applications (1)

Application Number Title Priority Date Filing Date
JP14813680A Granted JPS5772048A (en) 1980-10-24 1980-10-24 Component analyzing method

Country Status (1)

Country Link
JP (1) JPS5772048A (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS63271140A (en) * 1987-04-28 1988-11-09 Shimadzu Corp automatic rate analysis method
CN102680415A (en) * 2011-03-16 2012-09-19 中国农业科学院作物科学研究所 Colorimetric method for measuring the ketosuccinic acid content of the biological tissue

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS63271140A (en) * 1987-04-28 1988-11-09 Shimadzu Corp automatic rate analysis method
CN102680415A (en) * 2011-03-16 2012-09-19 中国农业科学院作物科学研究所 Colorimetric method for measuring the ketosuccinic acid content of the biological tissue

Also Published As

Publication number Publication date
JPH0121897B2 (en) 1989-04-24

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