JPS5772062A - Microquantitative determination of immunosuppressive acid protein - Google Patents

Microquantitative determination of immunosuppressive acid protein

Info

Publication number
JPS5772062A
JPS5772062A JP55148036A JP14803680A JPS5772062A JP S5772062 A JPS5772062 A JP S5772062A JP 55148036 A JP55148036 A JP 55148036A JP 14803680 A JP14803680 A JP 14803680A JP S5772062 A JPS5772062 A JP S5772062A
Authority
JP
Japan
Prior art keywords
iap
red blood
blood cell
antibody
diluted solution
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP55148036A
Other languages
Japanese (ja)
Inventor
Yoshimi Shibata
Keiji Tamura
Nakao Ishida
Tadayoshi Sato
Takashi Hirayama
Hide Kikuchi
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
SENDAI BISEIBUTSU KENKYUSHO
Original Assignee
SENDAI BISEIBUTSU KENKYUSHO
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by SENDAI BISEIBUTSU KENKYUSHO filed Critical SENDAI BISEIBUTSU KENKYUSHO
Priority to JP55148036A priority Critical patent/JPS5772062A/en
Publication of JPS5772062A publication Critical patent/JPS5772062A/en
Pending legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/575—Immunoassay; Biospecific binding assay; Materials therefor for cancer

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

PURPOSE:To accurately determine trace amounts of immunosuppressive acid protein (IAP) and to apply this method in cancer diagnosis, by method wherein an anti-IAP antibody, having a given concentration, is reacted with a specimen, especially a stepwise diluted solution of urine, and an IAP-sensitized red blood cell is then added to the reaction product to investigate the occurrence of agglomeration. CONSTITUTION:IAP taken from human body fluid is injected to a rabbit, and the obtained immune serum is incorporated into normal human serum to prepare anti- IAP antibody, which is the diluted to a given concentration to prepare a diluted solution of said antibody. Meanwhile, an IAP-sensitized sheep red blood cell is prepared. Said anti-IAP antibody diluted solution is added to a specimen (serum, abdominal dropsy, urine, etc.), especially that obtd. by the addn. of a stepwise diluted solution of urine and incubated at 37 deg.C for about 30min is prepd., and then IAP- sensitized red blood cell PBS-BS liquid is added thereto. After the solution is allowed to stand at a room temperature for 2hr, the occurrence of agglomeration of the red blood cell is checked. The occurrence of agglomeration shows the presence of IAP. This enables the sensitive and accurate detection of IAP which appears especially distinctly in a cancer patient.
JP55148036A 1980-10-22 1980-10-22 Microquantitative determination of immunosuppressive acid protein Pending JPS5772062A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP55148036A JPS5772062A (en) 1980-10-22 1980-10-22 Microquantitative determination of immunosuppressive acid protein

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP55148036A JPS5772062A (en) 1980-10-22 1980-10-22 Microquantitative determination of immunosuppressive acid protein

Publications (1)

Publication Number Publication Date
JPS5772062A true JPS5772062A (en) 1982-05-06

Family

ID=15443677

Family Applications (1)

Application Number Title Priority Date Filing Date
JP55148036A Pending JPS5772062A (en) 1980-10-22 1980-10-22 Microquantitative determination of immunosuppressive acid protein

Country Status (1)

Country Link
JP (1) JPS5772062A (en)

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS56132563A (en) * 1980-03-21 1981-10-16 Terumo Corp Reagent for testing biological material and use thereof

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS56132563A (en) * 1980-03-21 1981-10-16 Terumo Corp Reagent for testing biological material and use thereof

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