JPS5772919A - Buckwheat fluor extract and extracting method thereof - Google Patents
Buckwheat fluor extract and extracting method thereofInfo
- Publication number
- JPS5772919A JPS5772919A JP55149597A JP14959780A JPS5772919A JP S5772919 A JPS5772919 A JP S5772919A JP 55149597 A JP55149597 A JP 55149597A JP 14959780 A JP14959780 A JP 14959780A JP S5772919 A JPS5772919 A JP S5772919A
- Authority
- JP
- Japan
- Prior art keywords
- extract
- buckwheat
- buckwheat flour
- resultnat
- ion exchange
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 235000009419 Fagopyrum esculentum Nutrition 0.000 title abstract 5
- 238000000034 method Methods 0.000 title abstract 3
- 240000008620 Fagopyrum esculentum Species 0.000 title 1
- 241000219051 Fagopyrum Species 0.000 abstract 4
- 235000013312 flour Nutrition 0.000 abstract 3
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 abstract 2
- 238000002523 gelfiltration Methods 0.000 abstract 2
- 239000000126 substance Substances 0.000 abstract 2
- 239000004475 Arginine Substances 0.000 abstract 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 abstract 1
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 abstract 1
- 206010020751 Hypersensitivity Diseases 0.000 abstract 1
- AHLPHDHHMVZTML-BYPYZUCNSA-N L-Ornithine Chemical compound NCCC[C@H](N)C(O)=O AHLPHDHHMVZTML-BYPYZUCNSA-N 0.000 abstract 1
- CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 abstract 1
- AGPKZVBTJJNPAG-WHFBIAKZSA-N L-isoleucine Chemical compound CC[C@H](C)[C@H](N)C(O)=O AGPKZVBTJJNPAG-WHFBIAKZSA-N 0.000 abstract 1
- KZSNJWFQEVHDMF-BYPYZUCNSA-N L-valine Chemical compound CC(C)[C@H](N)C(O)=O KZSNJWFQEVHDMF-BYPYZUCNSA-N 0.000 abstract 1
- 239000004472 Lysine Substances 0.000 abstract 1
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 abstract 1
- AHLPHDHHMVZTML-UHFFFAOYSA-N Orn-delta-NH2 Natural products NCCCC(N)C(O)=O AHLPHDHHMVZTML-UHFFFAOYSA-N 0.000 abstract 1
- UTJLXEIPEHZYQJ-UHFFFAOYSA-N Ornithine Natural products OC(=O)C(C)CCCN UTJLXEIPEHZYQJ-UHFFFAOYSA-N 0.000 abstract 1
- 229920005654 Sephadex Polymers 0.000 abstract 1
- 239000012507 Sephadex™ Substances 0.000 abstract 1
- AYFVYJQAPQTCCC-UHFFFAOYSA-N Threonine Natural products CC(O)C(N)C(O)=O AYFVYJQAPQTCCC-UHFFFAOYSA-N 0.000 abstract 1
- 239000004473 Threonine Substances 0.000 abstract 1
- KZSNJWFQEVHDMF-UHFFFAOYSA-N Valine Natural products CC(C)C(N)C(O)=O KZSNJWFQEVHDMF-UHFFFAOYSA-N 0.000 abstract 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 abstract 1
- 235000001014 amino acid Nutrition 0.000 abstract 1
- 229940024606 amino acid Drugs 0.000 abstract 1
- 150000001413 amino acids Chemical class 0.000 abstract 1
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 abstract 1
- 235000003704 aspartic acid Nutrition 0.000 abstract 1
- OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 abstract 1
- 239000007853 buffer solution Substances 0.000 abstract 1
- 238000004440 column chromatography Methods 0.000 abstract 1
- 239000000470 constituent Substances 0.000 abstract 1
- 235000018417 cysteine Nutrition 0.000 abstract 1
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 abstract 1
- 238000000502 dialysis Methods 0.000 abstract 1
- 239000003814 drug Substances 0.000 abstract 1
- 238000001962 electrophoresis Methods 0.000 abstract 1
- 235000013922 glutamic acid Nutrition 0.000 abstract 1
- 239000004220 glutamic acid Substances 0.000 abstract 1
- 238000005342 ion exchange Methods 0.000 abstract 1
- 238000004255 ion exchange chromatography Methods 0.000 abstract 1
- 229960000310 isoleucine Drugs 0.000 abstract 1
- AGPKZVBTJJNPAG-UHFFFAOYSA-N isoleucine Natural products CCC(C)C(N)C(O)=O AGPKZVBTJJNPAG-UHFFFAOYSA-N 0.000 abstract 1
- 239000000203 mixture Substances 0.000 abstract 1
- 229960003104 ornithine Drugs 0.000 abstract 1
- GAPYKZAARZMMGP-UHFFFAOYSA-N pyridin-1-ium;acetate Chemical compound CC(O)=O.C1=CC=NC=C1 GAPYKZAARZMMGP-UHFFFAOYSA-N 0.000 abstract 1
- 239000000243 solution Substances 0.000 abstract 1
- 229940124597 therapeutic agent Drugs 0.000 abstract 1
- 239000004474 valine Substances 0.000 abstract 1
Landscapes
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
PURPOSE: To isolated and purify a haptenic substance useful as a hyposensitsation therapeutic agent for erethismic (hypersensitive) patients to buckwheat flour, by extracting a buckwheat flour with an aqeuou medium, dialyzing,the resultnat extract, and subjecting the dialyzate to the ion exchange chromatography, gel filtration, etc.
CONSTITUTION: A buckwheat flour is extracted witi an aqeous medium, and the resultnat extract is then separated and purified by the dialysis, ion exchange column chromatogrphy, gel filtrationand revers layer (phase) column chromatography. In the process, the measured vaue of the molecular weight according to the gel filtration method["Sephadex G50 " with a 1/30M phosphoric acid buffer solution (pH=6.5)]is about 1,600, and the substance is transferred to the cathodic side by the electrophoresis (carrier: filter paper No.51 manufactured by Toyo Roshi Kaisha, Ltd., pyridine-acetate bufrer solution, 600V, 2hr). The resultant buckwheat extract has the following amino acid composition: the weight ratio of aspartic acid> threonine: erine: glutamic acid: alaninie: valine: isoleucine: ornithine: lysine: arginine: cysteine=1:1:1:4:1:1:1:2:1:1:1, and the D-glucose is contaoned as a asccharide constituent.
COPYRIGHT: (C)1982,JPO&Japio
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP55149597A JPS5772919A (en) | 1980-10-25 | 1980-10-25 | Buckwheat fluor extract and extracting method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP55149597A JPS5772919A (en) | 1980-10-25 | 1980-10-25 | Buckwheat fluor extract and extracting method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPS5772919A true JPS5772919A (en) | 1982-05-07 |
Family
ID=15478679
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP55149597A Pending JPS5772919A (en) | 1980-10-25 | 1980-10-25 | Buckwheat fluor extract and extracting method thereof |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS5772919A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2702147A1 (en) * | 1993-03-05 | 1994-09-09 | Celbert Sa Groupe | Inhibitor of collagenase activity and cosmetic composition containing such an inhibitor. |
-
1980
- 1980-10-25 JP JP55149597A patent/JPS5772919A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2702147A1 (en) * | 1993-03-05 | 1994-09-09 | Celbert Sa Groupe | Inhibitor of collagenase activity and cosmetic composition containing such an inhibitor. |
| WO1994020541A1 (en) * | 1993-03-05 | 1994-09-15 | Groupe Celbert Sa | Collagenase activity inhibitor and cosmetic composition containing same |
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