JPS5774088A - Purification of estrase - Google Patents
Purification of estraseInfo
- Publication number
- JPS5774088A JPS5774088A JP15262580A JP15262580A JPS5774088A JP S5774088 A JPS5774088 A JP S5774088A JP 15262580 A JP15262580 A JP 15262580A JP 15262580 A JP15262580 A JP 15262580A JP S5774088 A JPS5774088 A JP S5774088A
- Authority
- JP
- Japan
- Prior art keywords
- estrase
- sodium chloride
- buffer solution
- support
- alanine
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000000746 purification Methods 0.000 title 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 abstract 6
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 abstract 4
- 239000011780 sodium chloride Substances 0.000 abstract 3
- 239000004471 Glycine Substances 0.000 abstract 2
- QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 abstract 2
- 241001465754 Metazoa Species 0.000 abstract 2
- 235000004279 alanine Nutrition 0.000 abstract 2
- 239000007853 buffer solution Substances 0.000 abstract 2
- 210000001835 viscera Anatomy 0.000 abstract 2
- 229920000936 Agarose Polymers 0.000 abstract 1
- 229920002307 Dextran Polymers 0.000 abstract 1
- 239000008055 phosphate buffer solution Substances 0.000 abstract 1
- 235000018102 proteins Nutrition 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 125000006850 spacer group Chemical group 0.000 abstract 1
Landscapes
- Enzymes And Modification Thereof (AREA)
Abstract
PURPOSE: Estrase obtained from internal organs of mammalian animals is simply and efficiently purified by utilizing its characteristic affinity to the residual group of glycine or alanine.
CONSTITUTION: Glycine or alanine is bonded through a spacer with a support such agarose or dextran and the resultant support is brought into contact with crude estrase obtained from internal organs of mammalian animals. Then, contaminating foreign proteins are eluted with a buffer solution free from sodium chloride, preferably a phosphate buffer solution of about 7.4pH, then, the estrase held on the support is eluted with a buffer solution containing less than 0.2M sodium chloride, preferably by the gradient method in which the concentration of sodium chloride gradually increases from 0 to 0.2M.
COPYRIGHT: (C)1982,JPO&Japio
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP15262580A JPS5774088A (en) | 1980-10-29 | 1980-10-29 | Purification of estrase |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP15262580A JPS5774088A (en) | 1980-10-29 | 1980-10-29 | Purification of estrase |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPS5774088A true JPS5774088A (en) | 1982-05-10 |
Family
ID=15544465
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP15262580A Pending JPS5774088A (en) | 1980-10-29 | 1980-10-29 | Purification of estrase |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS5774088A (en) |
-
1980
- 1980-10-29 JP JP15262580A patent/JPS5774088A/en active Pending
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