JPS5777958A - Affinitive adsorbent for affnity chromatography - Google Patents
Affinitive adsorbent for affnity chromatographyInfo
- Publication number
- JPS5777958A JPS5777958A JP55153315A JP15331580A JPS5777958A JP S5777958 A JPS5777958 A JP S5777958A JP 55153315 A JP55153315 A JP 55153315A JP 15331580 A JP15331580 A JP 15331580A JP S5777958 A JPS5777958 A JP S5777958A
- Authority
- JP
- Japan
- Prior art keywords
- adsorbent
- adenosine diphosphate
- combining
- affinitive
- ribose
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000003463 adsorbent Substances 0.000 title abstract 3
- 238000004587 chromatography analysis Methods 0.000 title 1
- XTWYTFMLZFPYCI-KQYNXXCUSA-N 5'-adenylphosphoric acid Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP(O)(=O)OP(O)(O)=O)[C@@H](O)[C@H]1O XTWYTFMLZFPYCI-KQYNXXCUSA-N 0.000 abstract 4
- XTWYTFMLZFPYCI-UHFFFAOYSA-N Adenosine diphosphate Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(COP(O)(=O)OP(O)(O)=O)C(O)C1O XTWYTFMLZFPYCI-UHFFFAOYSA-N 0.000 abstract 4
- 102000004190 Enzymes Human genes 0.000 abstract 2
- 108090000790 Enzymes Proteins 0.000 abstract 2
- PYMYPHUHKUWMLA-LMVFSUKVSA-N Ribose Natural products OC[C@@H](O)[C@@H](O)[C@@H](O)C=O PYMYPHUHKUWMLA-LMVFSUKVSA-N 0.000 abstract 2
- HMFHBZSHGGEWLO-UHFFFAOYSA-N alpha-D-Furanose-Ribose Natural products OCC1OC(O)C(O)C1O HMFHBZSHGGEWLO-UHFFFAOYSA-N 0.000 abstract 2
- 239000003446 ligand Substances 0.000 abstract 2
- 239000000126 substance Substances 0.000 abstract 2
- 229920000936 Agarose Polymers 0.000 abstract 1
- HMFHBZSHGGEWLO-SOOFDHNKSA-N D-ribofuranose Chemical compound OC[C@H]1OC(O)[C@H](O)[C@@H]1O HMFHBZSHGGEWLO-SOOFDHNKSA-N 0.000 abstract 1
- 101001066878 Homo sapiens Polyribonucleotide nucleotidyltransferase 1, mitochondrial Proteins 0.000 abstract 1
- 102000002681 Polyribonucleotide nucleotidyltransferase Human genes 0.000 abstract 1
- 241000589596 Thermus Species 0.000 abstract 1
- 238000001042 affinity chromatography Methods 0.000 abstract 1
- 238000005349 anion exchange Methods 0.000 abstract 1
- 230000001580 bacterial effect Effects 0.000 abstract 1
- 239000007788 liquid Substances 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 125000000548 ribosyl group Chemical group C1([C@H](O)[C@H](O)[C@H](O1)CO)* 0.000 abstract 1
- 238000000926 separation method Methods 0.000 abstract 1
- 238000001179 sorption measurement Methods 0.000 abstract 1
- 125000006850 spacer group Chemical group 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Enzymes And Modification Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Solid-Sorbent Or Filter-Aiding Compositions (AREA)
Abstract
PURPOSE:To make it possible to separate and purify with excellent purity a substance (enzyme or the like) capable of combining with adenosine diphosphate, by combining adenosine diphosphate used as ligand with a supporting body at specified positions of ribose. CONSTITUTION:Adenosine diphosphate used as ligand is combined with a supporting body such as an agarose derivative through the intermediary of a spacer -(CH2)-6 at positions 2'-C and 3'-C of ribose, whereby an affinitive adsorbent (shown by the formula) for affinity chromatography is prepared. This adsorbent is used, for instance, for separating and purifying, with high purity and without contaminating of other similar proteins or the like, polynucleotide phosphorylase which is an enzyme capable of combining with adenosine diphosphate obtained from a liquid extracted from crushed bacterial body of Thermus thermophyllus through adsorption and separation by using an anion exchange substance.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP55153315A JPS5777958A (en) | 1980-10-31 | 1980-10-31 | Affinitive adsorbent for affnity chromatography |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP55153315A JPS5777958A (en) | 1980-10-31 | 1980-10-31 | Affinitive adsorbent for affnity chromatography |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS5777958A true JPS5777958A (en) | 1982-05-15 |
| JPH0154993B2 JPH0154993B2 (en) | 1989-11-21 |
Family
ID=15559801
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP55153315A Granted JPS5777958A (en) | 1980-10-31 | 1980-10-31 | Affinitive adsorbent for affnity chromatography |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS5777958A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS58193929A (en) * | 1982-05-07 | 1983-11-11 | Mitsubishi Electric Corp | Multi-axis cooling device |
| WO1995009694A1 (en) * | 1993-10-02 | 1995-04-13 | Merck Patent Gmbh | Nucleotide-containing sorbent for affinity chromatography |
-
1980
- 1980-10-31 JP JP55153315A patent/JPS5777958A/en active Granted
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS58193929A (en) * | 1982-05-07 | 1983-11-11 | Mitsubishi Electric Corp | Multi-axis cooling device |
| WO1995009694A1 (en) * | 1993-10-02 | 1995-04-13 | Merck Patent Gmbh | Nucleotide-containing sorbent for affinity chromatography |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0154993B2 (en) | 1989-11-21 |
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