JPS58105902A - Fungal growth inhibitors and methods - Google Patents

Abstract

(57) [Summary] This bulletin contains application data before electronic filing, so abstract data is not recorded.

Description

[Detailed description of the invention] The present invention relates to a method for inhibiting the growth of fungi.

Especially dry rot fungus.
s) Noze (field 4BK applicable).

Traditionally, the eradication of dry rot has been done by treating eroded structures with M
It required thorough treatment of the '4 weave. This is because the fungi reach other nutritional sources, increasing the nutritional pace (f) of the wood.
This is because it can grow over a wide range from base 1 through plaster and brick.

Blight and other fungal growth in inaccessible areas of a structure can be inhibited by applying certain substances to the exposed mycelium or vegetative pace. These and certain other substances are also of interest for the purpose of protecting vegetative paces from fungal attack.

The present invention relates to a method for inhibiting the growth of fungi capable of growing on non-vegetative surfaces, the method comprising:
rrmslocate), incorporated into mycelium,
or both, and when it is the only nutrient source supplied to the fungus, it is a nitrogen-containing substance selected from substances that the fungus does not use for its growth; it is the mycelium of the fungus, or the nutrients on which the mycelium can grow. Including how to handle pacing.

Generally, nitrogen-containing substances are taken up by mycelium.

and the substances transported, but not taken up or transported therein, are effective in nutrient-based protection against erosion.

The present invention is particularly applicable to the treatment of wood, such as standing trees and tree stumps. The wood may or may not be eroded. The latter is of course for protection. Psoriasis fungi, especially Serpula lacrimans
acrimans) and the suppression of a/L/ mell7 e mell
ea7 )V mejsx -5e J shi7 :Ar
m1 l Iariel lamellea) and wood-erosive Basidiomycete (Basidiomycete)
The suppression of 1 is of particular interest.

The invention may also be applied to the treatment and protection of optical equipment, for example camera lenses and where fungi can spread catastrophically from vegetative paces in the lens barrel.

Compatibility for a given substance is confirmed by testing whether the substance is transported into the mycelium of a particular fungus without being metabolized. That is, the fungal sample was subjected to the following conditions: (a'1 without a nitrogen source);

(+3) They may be grown separately under conditions in which there is no nitrogen source other than the predetermined substance, and (c) in the presence of a type of nitrogen source that is known to be metabolizable.

The growth from (b) is equal to the growth from (a), and (
C) is suitable for use in the method of the invention, provided that the substance is not metabolized and no transport occurs in the mycelium. Transport is easily demonstrated by uptake of the substance occurring in one part of the mycelium and detection of the substance in a second part of the mycelium separated from the uptake part (e.g. by the use of sensitive analytical techniques such as chromatography). obtain.

The nitrogen-containing substance is suitably water-soluble, non-toxic and non-corrosive, preferably a non-metabolizable natural or synthetic amino acid (typically a non-protein amino acid) or a structural analog of a natural amino acid. in free form or in simple derivatives 1, such as salts.

Salts are alkaline salts, usually sodium or potassium or acid salts. The molecular structure of such analogs, which generally act as metabolic inhibitors (i.e., anti-metabolites or compounds that inhibit growth by composition for nitrogen available at the mycelial growth point) 1 are usually derived from natural amino acids by the addition and/or substitution of one group. Such groups generally consist of not more than 25 atoms, such as C1''-06 alkyl groups, aromatic groups such as a! The substance may be an antimetabolite of glutamine or glutamic acid, such as DL-methionine sulfoxide.

CH35OCH2CH2CH(NH2)Co2H1 or δ-hydroxygudine, HCt, HO-C(CH3)
(NH2).

CH2CH2C)12CH(NH2)Co2H-HCt
7? It may also be an anti-metabolite of C#7, X-partic acid, alanine or glycine. aminoisobutyric acid,
(CH3)2-C(NH2)Co2H (which is odorless, colorless and non-corrosive) or amine salts such as hydrochloric acid, or alkali metal salts are particularly preferred when inhibiting the growth of Serpula lacrimans.

Nitrogen-containing substances are usually applied to mycelium or erodible or non-erodible vegetative paces by painting, spraying or, in the case of non-eroding wood, soaking the wood with bath dissolution or dispersion of the active substance in a liquid medium (preferably aqueous). Applies by. It is best to mix the nitrogenous material with an energy source (usually a carbohydrate, typically sugar) to facilitate the uptake of the active substance by the mycelium. Alternatively or additionally, the nitrogen-containing material may be mixed with one or more growth promoters, such as minerals, to increase the surface area of the mycelium exposed to the treatment.

Another feature of the invention relates to a composition for inhibiting the growth of fungi capable of growing on non-vegetative surfaces, the composition being transported into the mycelium of the fungus and providing the only nutrient substance to the fungus. When it is a nitrogen source, it contains nitrogen-containing substances that are not used by fungi for growth.

The substance is combined with one or both of (a) an energy source that promotes uptake of the substance by the mycelium and (b) a growth promoter that increases the surface area of the mycelium.

Suitable energy sources include, for example, monosaccharides, trisaccharides (e.g. sucrose) or polysaccharides (e.g. cellulose), and suitable growth promoters include flavonoids and minerals (e.g. magnesium, calcium, iron, sulfates and phosphates). (e.g. KH2PO4), etc. The nitrogen-containing material is generally less than 10% by weight of the additive composition, and usually less than 1% by weight.The energy source is generally at a concentration of less than 20%, but preferably 10%. Below, it is typically 3% or less.

The concentration of the mineral source is generally less than 0.5% by weight.

Nitrogen-containing substances are generally used dissolved or dispersed in a liquid medium, but to reduce transportation and other costs, concentrates 1, for example, alone or as a mixed powder with an energy source and/or a growth promoter, can be used. May be packaged.

A suitable amount of solvent or dispersion medium (usually water) is added to make a composition suitable for use.

The present invention will be explained below with reference to Examples.

Example 1 Growth Inhibition: Serpula lacrimans mycelia were grown radially from a disk of II% malt extract bunches onto the dry surface of another empty veterinary dish. When colonies reach a diameter of 17.5a+, aminoisobutyric acid (AID) is added to the agar at a concentration of 10% by weight. The results are shown in Table-1. For comparison, the results when AIB was not added are also shown in Table 1.

Table 1 Example 2 Mycelia of Serpula lacrimans were inoculated into blocks of wood and grown on a non-vegetative surface. An aqueous solution containing AlB100~/- was dropped onto the block (0, 2, z), and growth at the mycelium end was suppressed for at least 8 months from the drop position.

Example 8 AIB against woody colonies of Serpula lacrimans
The effect of adding was tested. The procedure was the same as in Example 1, except that a colonized wood block was used in place of the colonized agar disk placed in the center of the empty dish. After the mycelium begins to spread across the bottom of the dish from the wood, AIBIJ) Omg
0.2-/- solution was added to half of the plate.

Results: The growth increase (1M2) after 7 months of addition of the inhibitor is shown in Table-2: Table-2 It can be seen that AIB reduces the spread from the wood nutrient base. The mycelium remains alive on the treated block, but its rate of spread is reduced. AIB has been shown to affect growth at a certain distance from the point of adaptation.

Example 4 Mycelia of Serpula lacrimans are grown radially from a disc of VC 1% malt extract agar on the dry surface of another empty bird dish. After 7 days, 0.05 - of MS aqueous solution of 10 mg/- of a degree is added to several discs, and at the same time untreated ones are kept as control. The results are shown in Table-3.

Table 3 Example 5 Example 4 except that the MS concentration was increased to 240 mf/, t
This was done in accordance with. Twenty days after inoculation, the average diameter of treated colonies was 21.7±1.5 cy2, and the average diameter of untreated colonies was 25±2.5 am2.

Procedural amendment (spontaneous) 1 Indication of the case Patent application No. 120451 of 1982 2 Name of the invention Fungal growth inhibitor and method for inhibiting 3 Person making the amendment Relationship to the case Patent applicant address London, England Varnish D.V.L., 66-74 Victoria Street Name National Research and Development Corporation Representative A. M. Nis Crespiy Nationality United Kingdom 4 Agent 5 Date of amendment order (voluntary) 7. Contents of amendment ■ 9 Detailed Description of the Invention Column (1) On page 8 of the specification, from line 7 at the end to line 6 at the end, "δ-hydroxylysine, MCI," is replaced with [N) 12. CH2-
Correct 2: C■(OH)CHCHCH(NH2)C02H1.

(2) In the third and fourth lines of the main text of Example 5 on page 14 of the specification, "cm2j" is corrected to "cmJ."

that's all

Claims (1)

[Scope of Claims] 1. A substance that is transported into and/or taken up by the mycelium of a fungus or a nutrient pace on which the mycelium can grow, A method for controlling fungi that can grow on non-nutrient surfaces, characterized in that when the substance is the only nitrogen source supplied to the fungi, the fungi are treated with a nitrogen-containing substance selected from substances that are not utilized for growth by the fungi. Growth suppression method. 2. The method according to item 1, wherein the nitrogen-containing substance is taken into the mycelium and transported into the mycelium. 3. The method according to item 1 or 2, wherein the wood nutrient base is treated with a nitrogen-containing substance. 4. The mycelium of the fungus is Serpula lacrimans (Scrp
ula lacrimans), Arm11laria mel lea'I or Bas idiomycete
ete). 5. The method according to any of the preceding items, wherein the nitrogen-containing substance is a free or salt type natural or synthetic amino acid or a structural analog of a natural amino acid. 6. The method according to item 5, wherein the nitrogen-containing substance is a free natural non-protein amino acid or a salt thereof. 7. The nitrogen-containing substance adds or replaces one group to the natural amino acid with a group having not more than 25 atoms. 6. A method according to claim 5, wherein the natural amino acid homolog in free or salt form is structurally derived therefrom. 8. The group is a C1-C6 alkyl group, an aromatic group, or a hydroxyl group. 2. The method according to item 1, wherein the group is a sulfoxide group, a sulfonic acid group, a nitro group, or a halogen. 9. The method according to item 1, wherein the nitrogen-containing substance is an antimetabolite of glutamine, glutamic acid, aspartic acid, alanine, or glycine. 10, including'! 10. The method according to item 9, wherein the elementary substance is DL-methionine sulfoxide or hydrochloric acid/δ-hydroxylysine. 11. The method according to item 5.32, wherein the nitrogen-containing substance is aminoisobutyric acid or (CH)C(NH2)CO2H in free or salt form. Formula 12: (CH)C(NH2') CO2Hf Indicates Rel 72 Treating the mycelium or vegetative pace of fungi with minoisobutyric acid to suppress the growth of Serpula lacrimans, Armillaria melea and also wood-eroding Hasidiomycete. Method. 13. Nitrogen-containing substances transported into the mycelium of fungi that are not used for fungal growth when they are the only nitrogen source supplied to the fungi. (a) Transport by the mycelium; an energy source that facilitates the uptake of substances and (b
) A growth inhibitor for fungi capable of growing on non-nutritive surfaces containing one or both growth promoters that increase the surface area of the mycelium. +4. 14. The inhibitor according to item 13, wherein the nitrogen-containing substance is a natural or synthetic amino acid or a natural amino acid analog in the free salt form. 15. Nitrogen-containing X! INI Niy Mi/ Isobutyric acid (CH
3) The inhibitor according to item 14, which is 2C(NH2)CO2H. 16 Items 13 to 1 containing 10% by weight or more of nitrogen-containing substances
The inhibitor according to any one of Item 5. 17. First, the concentration of the energy source does not exceed 20% by weight
Inhibitors according to items 3 to 16. 18. No. 17 where the energy source is a monosaccharide or trisaccharide
Inhibitors as described in section. 19. The inhibitor according to item 18, wherein the energy source is sucrose. 20. The first containing a growth promoter at a concentration not exceeding 1 stack ★
The inhibitor according to items 3 to 19. 21. A method of using the inhibitor according to item 13 containing a nitrogen-containing substance and an energy source in a relative amount such that the concentration of the energy source does not exceed the concentration of the nitrogen-containing substance when dispersed or dissolved in a solvent. 22. The method according to item 21, wherein the nitrogen-containing substance is aminoisobutyric acid and the energy source is a monosaccharide or trisaccharide. 23. The method according to item 22, wherein the energy source is sucrose.