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National Institute of Advanced Industrial Science and Technology AIST
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Priority to JP57102553ApriorityCriticalpatent/JPS58220695A/ja
Publication of JPS58220695ApublicationCriticalpatent/JPS58220695A/ja
Publication of JPS6132960B2publicationCriticalpatent/JPS6132960B2/ja
C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
C12N15/09—Recombinant DNA-technology
C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
C12N15/70—Vectors or expression systems specially adapted for E. coli
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The catalytic domain of the dihydrolipoyl transacetylase component of the pyruvate dehydrogenase complex From Azotobacter vinelandii and Escherichia coli: Expression, purification, properties and preliminary X‐ray analysis
Рекомбинантная плазмида, кодирующая гибридный белок-предшественник инсулина человека (варианты), штамм бактерий e.coli - продуцент гибридного белка-предшественника инсулина человека (варианты) и способ получения инсулина человека