JPS5931003B2 - Flow cell for liquid chromatograph detector - Google Patents
Flow cell for liquid chromatograph detectorInfo
- Publication number
- JPS5931003B2 JPS5931003B2 JP15706579A JP15706579A JPS5931003B2 JP S5931003 B2 JPS5931003 B2 JP S5931003B2 JP 15706579 A JP15706579 A JP 15706579A JP 15706579 A JP15706579 A JP 15706579A JP S5931003 B2 JPS5931003 B2 JP S5931003B2
- Authority
- JP
- Japan
- Prior art keywords
- flow cell
- glass tube
- tube
- liquid chromatograph
- wall
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 239000007788 liquid Substances 0.000 title claims description 4
- 239000011521 glass Substances 0.000 claims description 11
- 238000004811 liquid chromatography Methods 0.000 claims description 3
- 229910001220 stainless steel Inorganic materials 0.000 description 8
- 239000010935 stainless steel Substances 0.000 description 8
- 238000009792 diffusion process Methods 0.000 description 7
- MWPLVEDNUUSJAV-UHFFFAOYSA-N anthracene Chemical compound C1=CC=CC2=CC3=CC=CC=C3C=C21 MWPLVEDNUUSJAV-UHFFFAOYSA-N 0.000 description 4
- 238000010586 diagram Methods 0.000 description 4
- 239000004809 Teflon Substances 0.000 description 3
- 229920006362 Teflon® Polymers 0.000 description 3
- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 description 2
- -1 anthracene n-hexane Chemical compound 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000010828 elution Methods 0.000 description 1
- 238000011896 sensitive detection Methods 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
- G01N21/03—Cuvette constructions
- G01N21/05—Flow-through cuvettes
Landscapes
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Optical Measuring Cells (AREA)
Description
【発明の詳細な説明】
本発明は液体クロマトグラフィーにより被験液体の分離
溶出される成分のフローセル内での拡散をなくし該成分
の高感度検出するためのものである。DETAILED DESCRIPTION OF THE INVENTION The present invention is for highly sensitive detection of components to be separated and eluted from a test liquid by liquid chromatography by eliminating diffusion of the components within a flow cell.
現在広く使用されているフローセルはガラス管２に内径
０．２５ｍｍ〜０．５ｍｍの二段に縮径したテフロン或
いはステンレスチューブ１を第１図のように接続したも
のである。A flow cell that is currently widely used is a glass tube 2 connected to a Teflon or stainless steel tube 1 having an inner diameter of 0.25 mm to 0.5 mm, which is reduced in two stages, as shown in FIG.
しかし、このようなフロセルでは分析カラムより溶出さ
れた成分のフローセル内での拡散が大きく、検出感度の
低下が起る。また溶出時間の近接した成分はセル内の拡
散により相互の分離状態が悪くなわ定量出来なくなる場
合もある。以上の問題点を克服するため第２図のような
フローセルを発明した。分析カラムからの溶出液は内径
Ｏ、２ｍ７７ｌのステンレスチューブ１’へと入れられ
る。この接続チューブ１’の一端はガラス管２に対して
、テフロンでコーティングされたｏリング３でシールさ
れる。この場合ガラス管２中へとステンレスチューブ１
の先端を突入しこのチューブに半径方向になるべく多数
の孔ｂｃｄ・・・をあける。このステンレスチューブ１
’の外壁とガラス管２の内壁とは僅かな隙間のある様に
する。その結果溶出液はａからばか力でなく、ｂ、ｃ、
ｄからも流入されるが、ガラス管内の或る横断面に中心
開口を経て溶出された成分が達する時間と孔ｂｃｄを経
てこの横断面に溶出された成分が達する時間とは等しく
なければならない。又流出口についても全く同じ構造で
なければならない。この様に構成したフローセルを液体
クロマトグラフィー用けい光検出器に応用し第１図と第
２図のフローセルを組み込んだ同一の検出器で両セル内
の拡散を第３図のフローダイアグラムにより測定した。
定流量ポンプ４と試料注入器５とけい光検出器を第３図
のように接続し定流量ポンプ４でｎ−ヘキサンを送液し
続け、アントラセンのｎ−ヘキサン溶液の一定量を試料
注入器でＴよシ接続ライン中に注入する。注入されたア
ントラセンはけい光検出器６で検出されレコーダー８に
記録される試料注入器５からけい光検出器までのアント
ラセンの拡散が一定であるため、螢光検出器６内に配置
してあるフローセルの構造のちがいによる拡散がレコー
ダーに記録される。拡散の程度は、理論段数ｎを計算す
ることにより容易に比較できる。その結果第２図のフロ
ーセルでは、１６９８０ｎ／ｍ）第１図のフローセルで
は３３５０ｎ／ｍであつた。これによジ第２図の構造の
フローセルの拡散が少ないことが分つた。伺半径方向の
孔Ｂｃは斜め方向上方にしても好結果が得られる。However, in such a flow cell, components eluted from the analytical column diffuse widely within the flow cell, resulting in a decrease in detection sensitivity. In addition, components with close elution times may not be separated from each other due to diffusion within the cell, making it impossible to quantify them. In order to overcome the above problems, we invented a flow cell as shown in Figure 2. The eluate from the analytical column is introduced into a stainless steel tube 1' with an inner diameter of O and a diameter of 2 m and 77 l. One end of this connecting tube 1' is sealed to the glass tube 2 with an O-ring 3 coated with Teflon. In this case, the stainless steel tube 1 is inserted into the glass tube 2.
Plunge the tip of the tube into the tube and make as many holes bcd as possible in the radial direction. This stainless steel tube 1
There should be a slight gap between the outer wall of ' and the inner wall of the glass tube 2. As a result, the eluate does not flow from a to b, c,
d, but the time for the eluted component to reach a certain cross section in the glass tube through the central opening must be equal to the time for the eluted component to reach this cross section through the hole bcd. Also, the outlet must have exactly the same structure. The flow cell configured in this manner was applied to a fluorescence detector for liquid chromatography, and the diffusion within both cells was measured using the flow diagram shown in Figure 3 using the same detector incorporating the flow cells shown in Figures 1 and 2. .
Connect the constant flow pump 4, sample injector 5, and fluorescence detector as shown in Figure 3, continue to feed n-hexane with the constant flow pump 4, and inject a fixed amount of anthracene n-hexane solution with the sample injector. Inject into the T-side connection line. The injected anthracene is detected by the fluorescence detector 6 and recorded in the recorder 8. Since the diffusion of anthracene from the sample injector 5 to the fluorescence detector is constant, it is placed in the fluorescence detector 6. Diffusion due to differences in flow cell structure is recorded on a recorder. The degree of diffusion can be easily compared by calculating the number of theoretical plates n. The result was 16980 n/m for the flow cell shown in FIG. 2, and 3350 n/m for the flow cell shown in FIG. As a result, it was found that the flow cell having the structure shown in FIG. 2 had less diffusion. Even if the hole Bc in the radial direction is placed diagonally upward, good results can be obtained.
第１図は液体クロマトグラフの検出器用フローセルの従
来型を示す図、第２図は本発明に依る第１図と同様の図
、第３図は第１，２図フローセルの性能比較に用いた装
置の概略図である。
′
１・・・テフロンチユーブ、１・・・ステンレスチユー
ブ、２・・・ガラス管、ａ・・・ステンレスチユーブの
開口、３・・・Ｏリング、ｂ・・・ステンレスチユーブ
にあけた孔、ｃ・・・ステンレスチユーブにあけた孔。Figure 1 is a diagram showing a conventional flow cell for a liquid chromatograph detector, Figure 2 is a diagram similar to Figure 1 according to the present invention, and Figure 3 is used to compare the performance of the flow cells in Figures 1 and 2. FIG. 2 is a schematic diagram of the device. ' 1... Teflon tube, 1... Stainless steel tube, 2... Glass tube, a... Opening of stainless steel tube, 3... O ring, b... Hole drilled in stainless steel tube, c ...A hole drilled in a stainless steel tube.
Claims (1)
体クロマトグラフ検出器用フローセルに於いて該セルの
ガラス管２に分析カラムからの溶出液を全く同じ状態で
流入、流出する様、このガラス管の両端に於いて全く等
しく、ガラス管外壁とガラス管内壁との間に間隙をおい
て上記ガラス管中にチューブを突入し、然してチューブ
の中心開口より外壁に向けて多数の孔をあけた事を特徴
とする液体クロマトグラフ検出器用フローセル。1. In a flow cell for a liquid chromatograph detector that irradiates ultraviolet rays from a direction perpendicular to a thin glass tube 2, this glass tube is designed so that the eluate from the analytical column flows into and out of the glass tube 2 of the cell in exactly the same manner. The tube is inserted into the glass tube with a gap between the outer wall and the inner wall of the glass tube, which are completely equal at both ends, and a large number of holes are made from the center opening of the tube toward the outer wall. Characteristics of flow cells for liquid chromatography detectors.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP15706579A JPS5931003B2 (en) | 1979-12-04 | 1979-12-04 | Flow cell for liquid chromatograph detector |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP15706579A JPS5931003B2 (en) | 1979-12-04 | 1979-12-04 | Flow cell for liquid chromatograph detector |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS5679945A JPS5679945A (en) | 1981-06-30 |
| JPS5931003B2 true JPS5931003B2 (en) | 1984-07-30 |
Family
ID=15641443
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP15706579A Expired JPS5931003B2 (en) | 1979-12-04 | 1979-12-04 | Flow cell for liquid chromatograph detector |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS5931003B2 (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP4513053B2 (en) * | 2003-10-30 | 2010-07-28 | 日本精機株式会社 | Instrument device |
| CN104749299B (en) * | 2013-12-25 | 2016-08-24 | 中国科学院大连化学物理研究所 | A kind of large volume flow cell for liquid chromatogram fluorescence detector |
-
1979
- 1979-12-04 JP JP15706579A patent/JPS5931003B2/en not_active Expired
Also Published As
| Publication number | Publication date |
|---|---|
| JPS5679945A (en) | 1981-06-30 |
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