JPS5931442A - Electrolyte purification equipment - Google Patents

Abstract

PURPOSE:To obtain effectively a refined solution and to enable to analyze with high accuracy, by supplying an electric current between a reservoir tank of an electrophoretic equipment and two electrode cells connected to the tank, and removing impure ions in an electrolytic solution for refining filled in the reservoir tank. CONSTITUTION:To remove impurities in a leading liquid for electrophoretic analysis or a terminal liquid, an equipment wherein electrode cells are connected to a reservoir tank 8 for the leading liquid through relay pipes 10, 10' is used. First, stop valves 14, 14' of electrode cells 9, 9' for charging and a stop valve 13 of the tank 8 for discharging are opened, and the leading liquid containing no thickener is filled up in the tank 8. Next, the valves 14, 14' are shut, and the leading liquid containing the thickener is filled from a stop valve 12 for charging to pushout the liquid containing no thickener. An electric current is supplied from a DC electric source 11, and impure ions (both anions and cations) are removed. Thus, the refined leading liquid is filled up to the leading-electrode cell 2 and a sample charging part of a electrophoretic pipe 4. The terminal liquid is refined by connecting the refining equipment of electrolytic solution to the reservoir tank 7 of terminal liquid. By this method, an analysis with a high accuracy can be obtd.

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention relates to an electrolyte purification device, and more particularly to an electrolyte purification device capable of removing impurity ions in a leading liquid or a terminal liquid used in electrophoretic analysis.

The leading liquid or terminal liquid used for electrophoresis may be contaminated with impurity ions, and these impurity ions often have an adverse effect on electrophoretic analysis. In particular, the leading liquid contains hydroxyethylcellulose, hydroxypropylmethylcellulose, etc. to suppress and prevent electrode reactions and zone boundary disturbances.
Viscous agents such as hydroxyl-lopylcellulose, methylcellulose, polyvinyl alcohol, polyvinylpyrrolidone, and Triton X-100 (registered trademark) are added (
F. M. The paper “ISOTA” by Everaerts et al.
CHO-PHORESIS” 1976 USA, “J
our own of chromatography
(Refer to Library"vol4 p180-181)
However, these viscous agents generally have low purity (there is a problem that impurity ions are easily mixed in).

This invention was made in view of these circumstances, and its specific configuration includes a reservoir tank filled with a refining electrolyte, and two electrode tanks each connected to the reservoir tank. This is an electrolytic solution purification device configured so that impurity ions in a refining electrolytic solution filled in a reservoir tank can be removed by electrorefining by energizing both electrodes.

That is, in this invention, an electrode tank is attached to a reservoir tank that is filled with Ichika oil for use in the electrolytic solution, so that impurity ions in the electrolytic solution can be removed by electrorefining.

The present invention will be described in detail below based on embodiments shown in the figures. Note that this invention is not limited by this.

In FIG. 1, the electrolytic solution purification device (S) is configured to be integrated into the electrophoresis device (E), and includes a reservoir tank (8) for the leading liquid and a relay capillary tube (10) (10) for the reservoir tank. ’) connected through the electrode bath (
9) (9)', and a DC power supply (11) that supplies current to both of these electrode tanks. In addition, (12) and (13) are the opening/closing valves for leading liquid introduction and discharge installed in the leading liquid reservoir tank (8), and (14) and (14') are installed in the electrode tanks (9) and (9'), respectively. This is an on-off valve for introduction.

Therefore, the reservoir tank (8) is filled with a leading liquid containing a viscous agent, and the relay tubes (10) (10') and electrode vessels (9) (9') are filled with the same electrolyte composition as the previous leading liquid. Fill with a liquid that does not contain only the agent. In other words, first the introduction on-off valve (14) (14') and the discharge on-off valve (1
3) is opened and a leading liquid containing no viscous agent is filled into all of the electrode vessels (9) (9'), the relay tubes (10) (10'), and at least a portion of the reservoir tank (8). Next, the introduction on-off valves (14) (14') are closed, and the introduction on-off valve (12) is opened to transfer the leading liquid containing the viscous agent to the reservoir tank (8). Fill by extruding the leading liquid.

In this way, the leading liquid containing the viscous agent is transferred to the leading liquid reservoir tank (8).
After filling the leading liquid containing no viscous agent into the electrode chambers (9) and (9'), respectively, the DC power source (11
), impurity ions (both negative and positive ions) in the leading liquid containing the viscous agent in the reservoir tank (8) can be removed by electrical purification.

The thus purified leading liquid is filled into the leading electrode tank (2) and the sample introduction part (5) of the migration tube (4), while the terminal liquid is filled from the terminal liquid reservoir tank (7) to the terminal electrode tank (3) and the sample introduction part (5) of the migration tube (4). Sample introduction part (5
) Fill the electrophoresis tube (4) up to (4). and the Sample Investigator Department (
5) By injecting the sample and applying electricity to both electrode vessels (2) and (3) from the high voltage current power source (1), normal isokinetic electrophoresis analysis can be performed. This analysis eliminates the influence of impurity ions that are likely to be mixed in with the viscous agent in the leading liquid, and takes full advantage of the viscous agent's function (preventing disturbance of the zone boundary surface due to thermal diffusion and liquid flow) to achieve high accuracy. can be done. Further, by integrating the electrolytic solution purification device (S) into the electrophoretic analysis device (E), a series of automatic operations with electrophoretic analysis becomes possible, and there is no risk of re-contamination.

Of course, the electrolytic solution can be purified in advance using time such as nighttime.

Note that the electrolyte purification device having the above configuration can also be installed on the terminal liquid reservoir tank side.

Further, a device having a stirring function such as a magnetic stirrer may be installed in the purification electrolyte leap ink liquid. Of course, the DC power source (11) and the high vehicle pressure room current power source (1) shown in FIG. 1 can also be used in common. Furthermore, the installation positions of the on-off valves of the electrolyte purifier (S) can be changed as shown in FIG. 2. In other words, the discharge on-off valve is connected to the electrode tank (9a) instead of the leading liquid reservoir tank (8a).
(9'a).
2a) and discharge on/off valves (15a) (15'a) are opened and the leading liquid containing the viscous agent is transferred to the leading liquid reservoir tank (8a) and the relay capillary (10a) (10'a).
, and at least a portion of the electrode baths (9a) (9'a). Next, the introduction on-off valve (12a) is closed, the introduction on-off valves (14a) (14'a) are opened, and while the previously filled leading liquid containing the viscous agent is pushed out, the lead ink liquid that does not contain the viscous agent is extruded. Both electrode tanks (9a) (9'
a) can also be filled.

The relay thin tubes (10) (10') or (10a) (10'a) mentioned in the following examples may be replaced with filters, on-off valves, etc. if the liquids in both tanks to be relayed do not mix. can.

Here, for reference, we used the same electrophoresis device (
The results of the electrophoretic analysis performed in E) are shown.

(B) Apparatus (E) Sample capillary type isotachophoresis analyzer [IP-2A] (with potential gradient detector) Leading liquid: 0.01 mol Hydrochloric acid and 0.02 mol Amediol Terminal liquid: 0.01 mol β-alanine and barium hydroxide (pH 10.9) Electrophoresis tube: inner diameter 1.0 mmφ, length 40 mm, inner diameter 0.5
mmφ, length 150mm Transfer current: Switch from 200 μA (6 minutes) to 100 μA (B) Device (S) Connect a relay capillary tube with an inner diameter of 1.0 mmφ to the leading liquid reservoir tank attached to the device (E). An electrode tank with the same dimensions as the leading electrode tank was connected through the lead electrode tank.

(c) Experimental results (see Figure 3) A: Isokinetic electrophoresis analysis data of 100 μl of a 0.2% aqueous solution of PVA (polyvinyl alcoholone), which is a type of viscosity agent.

B: Data analyzed under the same conditions as A using 50 μl of electrically purified reading liquid as a sample.

The zone detected in B is the electrolyte (reagent).

impurities derived from solvents and dissolved carbon dioxide) and not from viscous agents. In B, the impurity zones ■, ■, ■, and ■ detected in A disappear, indicating that ionic impurities are effectively removed by electrophoresis.

[Brief explanation of drawings]

Fig. 1 is a functional explanatory diagram showing one embodiment of the electrolytic solution purification device according to the present invention, Fig. 2 is a functional explanatory diagram of main parts showing another embodiment, and Fig. 3 is a functional explanatory diagram showing an embodiment of the electrolytic solution purification device according to the present invention. It is a graph showing experimental results. (S)... Electrolyte solution purification device, (8)... Leading liquid reservoir tank, (9) (
9')... Electrode tank, (10) (10')... Relay tube, (11)... DC power supply. Agent Patent Attorney Shintabu Nogawa

Claims (1)

[Claims] 1. Equipped with a reservoir tank filled with a purification electrolyte and two electrode tanks each connected to this reservoir tank, and by energizing both electrodes, the purification electrolyte is filled into the reservoir tank. An electrolytic solution purification device configured to remove impurity ions therein through electrical purification.