JPS59501733A - Platelet storage methods and containers - Google Patents

Abstract

(57) [Summary] This bulletin contains application data before electronic filing, so abstract data is not recorded.

Description

[Detailed description of the invention] Platelet storage methods and containers limb blood field The present invention relates to the storage of blood components. More specifically, the present invention provides concentrated platelet Concerning storage in form.

Adequate amount of blood The use of blood as an alternative to whole blood is now widely accepted. For example, packed red blood blood to obtain the desired various blood components such as blood cells, blood cells, platelets and cryoprecipitate. The fluid can be collected and processed under sterile conditions. Next, individual components are limited. It can be stored for an extended period of time and used as desired. for example Platelet concentrates can be used to treat platelet deficiencies. Platelets are commonly used Since they are stored for a period of time before use, effective storage techniques are needed.

Generally preferred storage conditions for platelet concentrates are medical grade at approximately 22°C. Includes storage in polyvinyl chloride (PVC) hangs.

Some available platelet storage hangs are heat stabilized with epoxidized vegetable oil. Contains polyvinyl chloride material. Typical epoxy used as heat stabilizer for PVc Oxidized vegetable oils include epoxidized soybean oil and epoxidized linseed oil. for example, Such platelet storage Ha.

is described in Warner et al., U.S. Pat. No. 4,280,497. . One unit of platelet concentrate (the amount of platelet concentrate obtained from one unit of blood) is According to the African Blood Banks Association, those with at least 0.55 XlOu viable platelets Dew. Generally, one unit of platelets contains about 0.55X1Ou to about 1.5X10" It will contain platelet cells. Platelet cells obtained from one unit of blood in this way The amount of can vary widely. As used here, one platelet cell is used unless otherwise specified. The number of positions includes about 0.55 x 10" to about 1.5 x 10". 1 unit of normal human blood The absolute number of platelet cells present in the cell is approximately 2. OX can be as high as 1011 . Currently available platelet cell harvesting techniques generally approximate the total number of platelets present in whole blood. Allows for 80%, in some cases about 90%, yield. Improved harvesting techniques will be available in the future. It is anticipated that such improved techniques will become available, and that such improvements do not fall within the spirit or spirit of the invention. shall not affect the scope and claims.

The number of platelets present in a given container significantly affects platelet storage. 1 unit of blood The number of platelets collected from the Maximizes the storage time and viability of stored platelet cells, which generally vary between This specific number or range of platelets, or a large number or range of platelets, Proposes a platelet storage container and method that optimizes storage conditions for a range of platelet numbers. It is highly disappointing to be able to provide such services.

Honshuban Natsarajo According to one aspect of the present invention, certain types of storage conditions have a storage period of up to at least 5 days. was found to be more conducive to platelet viability over time. more specific Generally, platelet cells are within a certain range in terms of the number of platelet cells stored within them. When stored in a container with a carbon dioxide gas permeation transfer rate through the container that is Platelet production of stored cells discovered to result in improved platelet viability. The concentration decreases when the carbon dioxide permeation rate is not within this specified range. like this If the carbon dioxide gas movement through the container is too large or too small, it will be stored. It was found to be detrimental to platelet viability.

Generally, to optimize platelet storage and maintain acceptable levels of platelet viability 6, the container must satisfy the following range.

110≦R≦360 And furthermore, R is expressed in nanomolar C02X (min x 10u platelet cells - cmHgcO2) be done.

C is a unit conversion constant of 2.68XIO-4 (nanomole-seconds)/(CC-minutes). Ru.

is the CO2 gas permeation constant of the container material in units of .

SA represents the total surface area of the vessel at Cl11.

FT represents the thickness of the container material in cm.

Preferably, the container is 0.55 x 1011, :! :1.5 x 10” The above R-value requirement must be met for any platelet cell number between.

According to another aspect of the present invention, the waste transfer rate (GTR) of the container satisfies the following range. plus improved blood to maintain one unit of platelets at an acceptable viability level. A platelet storage container is provided.

85≦GTR≦180 Furthermore, GTR is in the unit of (nanomole)/(min-cm HgCQz)'. expressed.

C, PCO2, SA and FA are the same variables as in formula (1).

According to other aspects of the invention, a method for storing platelet cells is provided and includes the following: The method includes housing platelet cells in a container that is filled with a surrounding area.

110≦R≦360 , R, C, PCO2, SA and FT are variables in equation (1), and generally more than about 0.7'5 x 10 u platelet cells are stored therein. It will be done.

According to yet another aspect of the invention, a method is provided for storing one unit of platelet cells, the method comprising: comprising housing said unit of platelet cells in a container in which the following ranges are met: .

85≦GTR≦180 , and the variables are as defined above for equations (1) and (2).

According to yet another aspect of the invention, the invention is adapted to store platelets and is stored therein. A container is provided having a concentrate of platelet cells in which 110≦R≦360 , and the variables are as defined previously. Preferably, the container stores The cells will have at least 0.75' x 10 u of platelet cells.

According to another aspect of the invention, viable platelet cells suitable for intravenous use are stored therein. A container having a concentrated unit of cells is provided, and the container satisfies the following ranges: including.

85≦GTR≦180 T , and the variables are as defined previously.

In each of the above specific examples, the number of platelet cells to be stored in the container is Optimal storage with specific GTR for the range of platelet cell numbers that will be encountered As containers are provided, they can be expressed as a range of numbers. blood like this An optimized container for storing a unit of platelet cells is provided.

In accordance with yet another aspect of the invention, a given number or range of platelet cells is stored. storage under improved conditions such that acceptable levels of platelets are maintained. First, a method for manufacturing platelet storage containers of appropriate dimensions, volume, and thickness is available for J-use. It will be done. According to the method, a platelet storage container that satisfies the following range is provided with platelets. Manufactured to accommodate a number or range of numbers.

110≦R≦360 , and the variables are as defined previously. Preferably, it should be stored in said container. The number of platelets will be greater than about 0.75 x 10''.

Typically, the container will be constructed of a suitable type of flexible polymeric film material. . Preferred types of polymers include plasticized polyvinyl chloride compositions, polyethylene materials and and polyolefin materials.

Kaihetsu Nagori Hiro Evacuation Center The invention will be more fully understood by reference to the following detailed description and accompanying drawings. It will be easy to understand.

FIG. 1 is a plan view of a platelet storage container according to the present invention.

1 dish Kasahi Gai Referring to FIG. 1, a platelet storage hang 10 according to the present invention includes a platelet storage hang 10. A container is illustrated. The platelet storage bag 10 is made of the material it is made of and the container. A conventional structure may be used except for dimensions and thickness. Platelet storage bag as shown. The plug 10 includes an inlet port 14 to, for example, a blood donor hack (not shown). A length of flexible tubing 16 is connected.

Alternatively, tube 16 may communicate with the interior of another container (not shown).

Also shown, the container has a number of normally sealed, selectively openable accessories. Includes Sport 12.

As previously discussed, the container satisfies the range 110≦R≦360.

Preferably R' is greater than 120, more preferably R is greater than 140 . Most preferably R is greater than 160. Preferably R is less than 270; Most preferably R is less than 210.

Gas transfer rate for storing one unit of platelets according to the containers and methods of the invention GTR is in the range 85≦GTR≦180 as previously discussed. Preferably G TR is 100≦GTR≦170. And more preferably 120≦GTR≦17 It is within the range of 0. The most preferred range of GTR for storing one unit of platelet cells The range is about 160 to about 170.

According to the present invention, the optimum number of platelet cells for storing a given range of platelet cells A platelet storage container can be provided.

The GTR of the container is 110≦R≦ for the entire range of platelet cells that can be stored within the container. 360 and more preferably in the range 120≦R≦270. A container is provided. For example, if about 0゜5　X　1011 to 1.5　X　1 For storage of one unit of platelet concentrate, which can generally contain 0.11 platelet cells, If a suitable container is desired, a container with a GTR of 165 has 1.5 When 1011 platelets are stored, 110 R and 0.5 x 10” platelets are stored. of platelets will have an R of 330 when stored. If the container is 0.75X1 Optimized for a narrow range of platelet cell counts such as 1” to 1.25XIO11 If the I can do it. For example, platelet cells in a container with a GTR of between about 150 and 180 The storage capacity is 0.75 x 1011 to 1.25 x 11 platelet cells. When the cells are stored they will always give an R value in the range 120≦R≦240. child As such, the present invention may be encountered when obtaining multiple units of platelet concentrate from multiple units of blood. Optimize platelet storage containers for storage of a wide range of platelet cells, such as allow.

Platelet storage bag 10 may be constructed of any suitable type of material. for example , plasticized polyvinyl chloride resin compositions, polyethylene materials and polyolefins material can be used. Such materials are important because the resulting material stores platelets. known to those skilled in the art, as long as the value of R or GTR is within the desired range. can be plasticized and heat stabilized as desired by any material.

Other types of materials, polymeric or non-polymeric, as long as gas transfer requirements are provided - can also be used. As long as the overall gas transfer requirements are met in this way, Combinations of materials can be used, and materials with various thicknesses can be used. example If the overall gas transfer requirements are met, the vessel is very thick and relatively non-porous. The other parts of the container could be relatively porous. book like this The invention is not limited to a particular material or design, or to a material of uniform thickness. Not possible. Polymers and polymer films are generally useful in the practice of the present invention .

For example, a preferred type of polyvinyl chloride composition is plasticized PVC resin, PvC. an effective amount of tri-2-ethylhexyl trimellitate to less than about 1% of the amount of a thermal stabilizing system, and optionally an effective amount of an anti-blocking agent. .

As will be appreciated, the amount of plasticizer is selected to provide the desired degree of flexibility. . Compositions containing relatively low amounts of plasticizer may be harder and less flexible than desired. compositions with relatively high amounts of plasticizer may be softer and more flexible than desired. can be obtained. Generally, as the amount of plasticizer increases, mixing becomes more difficult, and the plasticizer As leaching increases and the amount of plasticizer decreases, gas permeability generally decreases; is undesirable for platelet storage. Generally of plasticizers for this type of composition An effective amount will be about 25 to 90 parts per 100 weight resin. Preferably plastic The amount of agent is about 63 to 85 parts per 100 parts resin, most preferably about 74 parts. It is.

The heat stabilizing system should generally be present in an amount greater than about 0.15% by weight of the total composition. and preferably about 0.26 to 0.35 parts per 100 parts by weight of resin. present in amounts between . Generally, increased leaching occurs as the amount of heat stabilizer increases However, if the amount of heat stabilizer is high enough, haze can occur.

Generally, the specialty blocking agents used are formulated to give the desired anti-blocking effect. is then present in an amount sufficient to prevent sticking of films made from the composition. Will. The amount of antiblocking agent is typically from 0.2 to about 0 per 100 parts by weight of resin. ．． 6 parts, and preferably about 0.4 parts. Again the amount of wax generally increases As time passes, increased leaching occurs. For this reason, the preferred amount of antiblocking agent is generally It is the lowest amount that will prevent films made from the composition from sticking.

A particularly preferred composition is a PVC resin resin 100 tri-2-ethylhexyl 74 Department, Interstab Company under the trade name CZ-11-P Commercial Calcium Stearate and Zinc Stearate Heat Stabilizers 0. Part 3, and Glyco Chemicals.

Inc. Wax anti-brown sold under the trade name Acrawax C by Cooking agent 0. There are 4 parts. Such a composition has a temperature of about 49 bar at 25°C. has a file.

The components of the composition can generally be suitably mixed, for example, in a blender. The composition is into sheets by conventional methods such as rendering or extrusion to the desired thickness can be formed. Mixing screw type equipment is used to extrude the material be able to.

In general, materials should be prepared in a suitable manner such that unacceptable thermal degradation or discoloration of the material does not occur. Processed with conditions. The type and amount of heat stabilizer is such that the composition does not significantly deteriorate or change. This will affect the processing conditions it can be subjected to without causing color. example For example, a PVC composition without calcium stearate is The color may become undesirably darker during processing than compositions containing zinc stearate and zinc stearate. I try to sleep.

The polyvinyl chloride resin may be of any suitable type. Absorbs plasticizer relatively easily A type of PVC resin is preferred. A particularly preferred resin is known as blotting paper resin. It is a resin that can be used. Such a preferable 414 fat (7) - it hahi, e Sold under the product name Zeon 80X80 by Fu, Gufdorinochi, Chemical, and Company. has been done. A preferred resin is Acker Co. Product name by VINN It is sold as OL H700F.

The plasticizer used according to the invention, tri-2-ethylhexyl trimellitate, is commercially available. Commercially available. One source is 1 (atc.

Chemical Corp. It is a plasticizer sold under the trade name Hatcol 20. Ru.

As previously discussed, the thermostabilized calcium stearate, zinc stearate or can be a mixture thereof. More generally, the thermally stabilized system is C1o - It can be a salt of at least one type of C26 saturated fatty acid. stearic acid A 1:.1 weight ratio of lead/calcium stearate is preferred. Zinc stearate/ One suitable commercial source of calcium stearate is a heat stabilizer such as Interstab Compan sells the product under the product name CZ-11-P. It is y.

CZ-11-P contains small amounts of calcium valmitate, zinc palmitate and C1 It may contain 8 fatty acids.

Anti-blocking agents that may be present generally provide the desired anti-blocking properties without an undesirable degree of leaching. Any substance that provides the effect and is otherwise acceptable may be used. Preferred anti-block The agent was manufactured by Glyco Chemicals, Inc. Product name by Acraw It is a low viscosity, high melting point wax such as the wax sold under the trademark AX. high Viscosity oil can also be used. However, oils generally have a higher degree of impregnation than waxes. It will be served. Mixtures of antiblocking agents can also be used. like that For a more complete description of PVC compositions, see U.S. Pat. See patent application no. 424,679. Other pvc compositions include epoxy, which acts as a heat stabilizer. May contain oxygenated vegetable oils. Such compositions are described by Harner et al. of No. 4,280,497, which we refer to for further explanation. illuminate However, the spirit and scope of the invention is limited to particular materials or compositions. It should be noted that this is not specified.

Generally, the thickness of the polymeric material is about 0.01 cm and 0.01 cm. Must be between 0.5 cm cormorant.

Other platelet storage materials may also be used, including those previously described and others known to those skilled in the art. can do.

The present invention will be further understood by referring to the following examples. To determine the effect of R and GTR on platelet viability during 3 and 5 days of storage. To demonstrate, experiments were conducted on various types of platelet storage bags. platelet raw The effect on survival is that pH optimizes final in vivo platelet survival and function after injection. It is generally accepted that the indication is - determined by the pH of stored platelets. did. Generally, the pH of stored platelets is kept within the range of about 6.5 to about 7.2 over the storage range. It is hoped that

The following materials and storage hang types were used:

To Lin Shou Type: Polyolefin PCO2, 25℃: 58bar Bag volume/surface area 300 relatives/365 cal 1000IR1, /644cII+ Thickness: 300d bag: o, 03cm 1000cm bag: 0. O4 50 A good pillar Type: PVC T’COz, 25°C: 49bar Hang volume/surface area 300m! Q/280 cIl1 1000 dai / 398 cnl Thickness: 300yd bag: 0.015cm 1000d bag: 0.015cm Hayashiryotan Guip: Polyvinyl chloride PCO2, 25℃: 30bar Bag volume/surface area 300 relatives/280 cni Thickness: 0.038cm Platelet concentrate can be extracted from a single unit of blood or by drawing blood continuously to remove platelets. The blood was obtained from a continuous centrifugation system that was centrifuged to remove blood and returned to the donor. blood The platelets were stored at ↓ below the ambient temperature, and substandard results were obtained after approximately 3 and 5 days of storage. It was done.

(Margin below) 3 and 5 day storage Thus, the above results indicate the critical nature of the R and GTR parameters. . Platelets stored under conditions where R is in the range lOO≧R≧36.0 are relatively uniform. The pH was generally between 6.5 and 7.2 after 3 and 5 days of storage. Platelets stored under conditions where R is not within the range of 100≧R≧360 are much larger. pH instability and generally significantly higher or lower than the range 6.5 to 7.2 It showed a high pH value. 3-day and 5-day GTR is within the range of 85≦GTR≦180 Platelet units (approximately 0.5 to 1.5 x 10 u) stored under certain conditions (platelet cells) exhibit a relatively stable pH, which generally ranges from 6.5 to 7.2. Met. Like that! Units not stored within GTR range 6.5 to 7.2 The 11IH value was outside the range of .

Although the invention has been described with respect to preferred embodiments thereof, it is contemplated that variations, modifications and variations may occur. It is understood that variations are possible within the scope of the claims.

Claims (1)

[Claims] 1. The container is made of a material within the range of 85≦GTR≦180. Platelet capacity to store one unit of platelets in a cell at an acceptable survival rate for a certain period of time. vessel: T and GTR is Na/mo) L/CO2/min-cmHgcOz (in D units, C represents the constant 2.68 x 10-4 nanomolar seconds/cc-min, in units of The CO2 gas permeation constant of the material is expressed as crA, and the record is the total surface area of the container expressed as crA. and FT represents the thickness of the material in cm. 2. The container has a platelet storage capacity of item 1 within the range of 100≦GTR≦180. vessel. 3. The platelet storage container of item 1, wherein the container is within the range of 120≦GTR≦170. . 4. The platelet storage container of item 1, wherein the GTR of the container is about 160 to 170. 5. The material is made of polymer material, and is made of polyvinyl chloride, polyethylene or and polyolefin. 6. The platelet storage capacity of item 1, wherein the volume of the container is about 300 to about 600 mJ2. vessel. 7. The platelet of item 1, wherein the thickness of the material is about 0.01 cm to about 0.05 cm. storage container. 8. Container is 1 for platelet cell number between 0.55X10u and 1.5X10" A container made of a certain material within the range of 10≦R≦360. improved blood cells to store approximately 1 unit of platelets to acceptable viability levels. Plate storage vessel: R is nanomoles CO2/(min Xl0-4 platelet cells-cm1gcO 2), C represents the constant 2.68 x 10-4 nanomolar seconds/cc-min, in units of represents the CO2 gas permeation constant of the material at FT stands for the thickness of the material in countries. 9. The container is within the range of 160≦R≦270, and the container has a diameter of 1.20 Platelet storage according to paragraph 8, which is for storing more than X'lO'' platelet cells. Storage container. 10, the container is within the range of 160≦R≦210, and the container has a diameter of 1.20 Platelet storage according to paragraph 8, which is for storing more than X10u platelet cells. container. 11. The materials include polyvinyl chloride, polyethylene and polyolefins. 9. A platelet storage container according to clause 8, comprising a flexible polymeric material selected from the group consisting of: 12. The blood cell according to paragraph 8, wherein the thickness of the material is about 0.01 cm to about 0.05 cm. Board storage container. 13. GTR for units of platelet cells in which the container is within the range of 85≦GTR≦180 consists of housing platelet cells in a container made of a certain material with a ratio of , a method for storing platelets under optimal conditions: T and GTR is in nanomoles C02/min-cmHgcO2, where C is the constant 2. 68 X represents the unit of 10-4 nanomolar seconds/cc-minute, and the front in the unit of 002 gas permeability constant of the material, and S^ is the total surface area of the container expressed as cn1. where FT represents the thickness of the material in units. 14. The method of item 13, wherein the container is within the range of 100≦GTR≦180. 15. The method of item 13, wherein the container is within the range of 120≦GTR≦170. 16. The method of clause 13, wherein the container has a GTR of about 160 to 170. 17. The material consists of polyvinyl chloride, polyethylene and polyolefin. 14. The method of clause 13, comprising a polymeric material selected from the group. 18. The method of clause 13, wherein the container has a volume of about 300 to about 600 d. 19. The method of clause 13, wherein the material has a thickness of about 0.01-0.05 cm. . 20. Blood in a container made of a certain material, where the container is within the range of 110≦R≦360. A method for storing platelet cells in optimal conditions, comprising housing platelet cells: R represents nanomoles CO2/(min x 10u platelet cells - cmHgcOz), C represents the constant 2.68 x 10-4 nanomolar seconds/cc-min, in units of represents the CO2 gas permeation constant of the material at - the total surface area of the container represented where FT represents the thickness of the material in countries. 21. The method of item 20, wherein the container is within the range of 120≦R≦270. 22. The method of item 20, wherein the container is within the range of 160≦R≦210. 23. The material is made of polyvinyl chloride, polyethylene and polyolefin. Clause 20, wherein said material has a thickness of approximately 20. The method of clause 20, wherein the diameter is 0.01 cm to about 0.05 an. 25. The method of clause 20, wherein at least about 0.5 x 10 u of blood plates are stored. 26. Any of paragraphs 20 to 25, in which platelets are stored in concentrated form. Method. 27. Containers made of a certain material within the range of 8,5≦GTR≦180 1 unit of concentrated viable platelet cells suitable for intravenous use stored therein Container with: T and GTR is in nanomoles C02/min-cmHgcOz, where C is the constant 2. 68 X I O-4 nanomolar seconds/cc-min, the above material in units of represents the CO2 gas permeation constant of the material; SA represents the total surface area of the container; F T represents the thickness of the material expressed in (2). 28. The container stores the platelets of paragraph 27 in the range of 100≦GTR≦180. Combination of container and platelet concentrate. 29. The platelet storage capacity of paragraph 27, wherein the container is within the range of 120≦GTR≦170. A combination of a device and a platelet concentrate. 30. The platelet storage container of paragraph 27, wherein the container has a GTR of about 160 to 170. Combination with platelet concentrate. 31. The material is made of polyvinyl chloride, polyethylene and polyolefin. Platelet storage container and platelet concentrate according to paragraph 27, comprising a polymeric cane material selected from the group combination with. 32. The platelet storage capacity of paragraph 27, wherein the volume of the container is about 300 to about 60 (7). A combination of a device and a platelet concentrate. 33. The thickness of paragraph 27, wherein the thickness of the material is from about 0.01 cm to about 0.05 c+n. Combination of platelet storage container and platelet concentrate. 34. For platelet cell counts where the container is between 0.55X10" and 1.5X10" made of and stored in a material in the range 110≦R≦360 Contains a concentrate of at least 1 unit of platelet cells suitable for intravenous use , Container suitable for platelet storage: R is nano/I/CO2/(min. plate cell-, cmHgCO2), C represents the constant 2.68 x 10 → nanomolar seconds/cc-min, in units of represents the COz gas permeation constant of the material in which SA is the total surface area of the container represented. where FT represents the thickness of the material expressed in ■. 35. The container is within the range of 160≦R≦270, and the container has 1. Blood according to paragraph 34, which is for storing more than '20 x 10'' platelet cells. Combination of platelet storage container and platelet concentrate. 36, the container is in the range 160≦R≦210, and the container has a diameter of 1.20 Platelet storage according to paragraph 34, which is for storing more than 10" platelet cells. Combination of storage container and platelet concentrate. 37. The material is made of polyvinyl chloride, polyethylene and polyolefin. 35. The combination with a platelet concentrate of paragraph 34 consisting of a polymeric material selected from the group consisting of: 38. The material of paragraph 34, wherein the thickness of the material is from about 0.01 cm to about 0.05 cm. Combination of platelet storage container and platelet concentrate. 39.The container is made of a certain material, and the container is 0.75×10” and 1.5×101 110≦R≦360 for platelet counts between 1 and at least about Modifications for storing 0.75X10u platelet cells at acceptable viability levels. Improved platelet storage container: (FT) number Xl0-11), R represents nanomolar COz/(min x 10u platelet cells - cm1gcO2); C represents the constant 2.68 x 1'0-4 nanomolar seconds/cc-min, in units of represents the COz gas permeation constant of the material in which SA is the total surface area of the container represented. where FT represents the thickness of the material in terms of thickness. 40, the container is within the range of 120≦R≦270, and the container has 1. Blood cells according to paragraph 39 for storing more than 20 x 10" platelet cells Board storage container. 41, the container is in the range 160≦R≦210, and the container has a diameter of 1.20 The platelet store according to paragraph 39, which is intended to store more than X10u platelet cells. Storage container. 42. The material is made of polyvinyl chloride, polyethylene and polyolefin. 40. The platelet storage container of clause 39, comprising a polymeric material selected from the group consisting of: 43. The thickness of paragraph 39 is from about 0.01 cm to about 0.05 cm. Platelet storage container. 44. Platelet storage where the container is in the range lIO≦R≦360 and is made of a certain material at least about 0.75 x 10'' platelet cells passed through and stored therein. Container you have】R is nanomolar CO2/(min x 10u platelet cells - cmHgc O2), C represents the constant 2.68 x 10-4 nanomolar seconds/cc-min, in units of represents the COz gas permeation constant of the material at - the total surface area of the container represented and FT represents the thickness of the material in cm. 45. The platelet storage capacity of paragraph 44, wherein the container is within the range of 120≦R≦270. A combination of a device and a platelet concentrate. 46. The platelet storage capacity of paragraph 44, wherein the container is within the range of 160≦R≦210. A combination of a device and a platelet concentrate. 47. The material is made of polyvinyl chloride, polyethylene and polyolefin. Platelet storage container and platelet concentrate according to paragraph 44, comprising a polymeric material selected from the group consisting of: combination with things. 48. The blood of paragraph 44, wherein the thickness of the material is about Q, 01 cm to about 0.05 cm. Combination of platelet storage container and platelet concentrate. 49. The container is made of a certain material, and the container is within the range of 160≦R≦360. , at least about], optimal conditions for maintaining the viability of 20XlOu platelets. Improved platelet storage (FT) the number of platelet cells Xl0−”), R is nanomoles CO2/(min x l Q 11 platelet cells - cmHgcOz) represents, C represents the constant 2.68 x 10-4 nanomolar seconds/cc-min, in units of represents the C02 gas permeation constant of the material at - the total surface area of the container represented where FT represents the thickness of the material in countries. 50. Containers made of a certain material within the range of 200≦GTR≦420 can accommodate approximately 1.30XIO" to approximately 3.5X10u platelets. Platelet storage containers for storage for a period of time at viability levels: T and GTR is in units of nanomoles CO2/min-HgCO2, where C is the constant 2.6 of the said material in units of 8 x 10-4 nanomolar seconds/cc-min. represents the CO2 gas permeation constant, SA represents the total surface area of the container in cTA, F T represents the thickness of the material represented by ■. 51, the container stores about 3.5 x 1011 to about 9.4 x lOu platelets and the container is within the range of 538≦GTR≦1130 Platelet storage container according to paragraph 50. 52, the container has 11 platelet cells stored therein [1!8.5 per 1 Manufactured from a certain material within the range of 10-10≦GTR≦3.3 x 10-9 A container capable of containing more than 1.30 x 1011 platelets. A platelet storage container for storing platelet levels for a period of time: GTR is in nanomoles C02/min-cmHgcOz, where C is the constant 2. The said material in units of 68 x 10-4 nanomolar seconds/cc-min. represents the CO2 gas permeation constant of , SA represents the total surface area of the vessel represented by -, and FT represents the thickness of said material in marks.