JPS60203848A - Isokinetic electrophoresis device - Google Patents
Isokinetic electrophoresis deviceInfo
- Publication number
- JPS60203848A JPS60203848A JP59061943A JP6194384A JPS60203848A JP S60203848 A JPS60203848 A JP S60203848A JP 59061943 A JP59061943 A JP 59061943A JP 6194384 A JP6194384 A JP 6194384A JP S60203848 A JPS60203848 A JP S60203848A
- Authority
- JP
- Japan
- Prior art keywords
- sample
- electrophoresis
- leading
- ion
- value
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
- G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
- G01N27/4473—Arrangements for investigating the separated zones, e.g. localising zones by electric means
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Molecular Biology (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Electrochemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
Abstract
(57)【要約】本公報は電子出願前の出願データであるた
め要約のデータは記録されません。(57) [Summary] This bulletin contains application data before electronic filing, so abstract data is not recorded.
Description
【発明の詳細な説明】
（イ）産業上の利用分野
この発明は９等速電気泳動装置をこ関し、特に試料分析
時をこおける試料イオンの泳動管内の移動位置を検出で
きるよう蚤こした細管式等、速電気泳動装置に関するも
のである。[Detailed description of the invention] (a) Industrial application field The present invention relates to a 9 isokinetic electrophoresis device, and is particularly designed to detect the movement position of sample ions in the migration tube during sample analysis. This relates to fast electrophoresis devices such as capillary type.
ｐ）従来技術 従来の細管式等速電気泳動装置においては。p) Conventional technology In the conventional capillary isotachophoresis device.
試料イオンの泳動開始から試料イオンが検出器に検出さ
れるまでの間は、検出器がリーディング電解液のイオン
、すなわちリーディングイオンを検出するだけで、チャ
・−ト紙上蚤こ試料イオンが検出されていない状態を示
すベースラインが描かれるだけである。そのため試料イ
オンゾーンが検出密にとの程度近づいているのか、どの
位の時間で泳動中の試料の分析が済むのかなどの情報が
得られなかった。From the start of sample ion migration until the sample ions are detected by the detector, the detector only detects the ions in the leading electrolyte, that is, the leading ions, and the sample ions on the chart paper are not detected. Only a baseline is drawn to indicate the state in which the data is not present. Therefore, it was not possible to obtain information such as whether the sample ion zone was approaching the detection density or how long it would take to complete the analysis of the sample being migrated.
そこで、試料イオンの泳動時間を予め測定すること・こ
よって上記情報を得るようにしているが１分析操作が二
度手間となり作業能率が悪くなるものである。Therefore, the above-mentioned information is obtained by measuring the migration time of the sample ions in advance, but one analysis operation becomes twice the effort, resulting in poor work efficiency.
（ハ）目的
この発明は上記従来装置−こおける分析操作の二度手間
といった煩雑さを解消するためをこなされたものであり
９．試料分析者が試料分析中をこ泳動管内の試料イオン
ゾーンの先端位置を知ることができるようにした等速電
気泳動装置の提供を目的とする。(c) Purpose This invention has been accomplished in order to eliminate the complication of having to perform analysis operations twice in the conventional apparatus described above.9. An object of the present invention is to provide an isotachophoresis apparatus that allows a sample analyst to know the position of the tip of a sample ion zone in a migration tube during sample analysis.
に）構成
この発明をこ係る等速電気泳動装置は、泳動管路申告こ
電解液のＰＨ値を測定するためのＰＨ測定手段と、・こ
のＰＨ測定手段からの出力により泳動状態を表示するた
めの表示手段とを備えたことを特徴とし、電解液のうち
あらかじめ既知のリーディング液ＰＨ値を追跡すること
によって、試料イオンゾーンの先端位置を知ることがで
きるように構成されている。B) Structure The isotachophoresis apparatus according to the present invention includes a PH measuring means for measuring the PH value of the electrolyte in the electrophoresis tube, and a means for displaying the electrophoresis state by the output from the PH measuring means. The present invention is characterized by a display means, and is configured such that the position of the tip of the sample ion zone can be known by tracking the pH value of the leading solution, which is known in advance among the electrolytes.
（ホ）実施例
以下図面に基づいてこの発明の実施例である等速電気泳
動装置について説明する。(E) Example An isokinetic electrophoresis apparatus which is an example of the present invention will be described below with reference to the drawings.
第１図は細管式等速電気泳動装置の全体構成を示す説明
図である。泳動管（５）の−万端側に試料注入室（６）
を介してターミナル電極槽（３）と、その他方端側に検
出器（７）を介してリーディング電極槽（４）とがそれ
ぞれ設置されている。FIG. 1 is an explanatory diagram showing the overall configuration of a capillary type isotachophoresis device. Sample injection chamber (6) on the - end side of the electrophoresis tube (5)
A terminal electrode tank (3) is installed at the other end via a detector (7), and a leading electrode tank (4) is installed at the other end.
試料注入室（６）は、リーディング液とターミナル液の
境界面を形成するとともに、試料を２液の界面・こ注入
できる構造になっており、定電流電源（２）ヲこより両
電極槽ｆ３１ （４１に高電圧を印加すれば、リーディ
ングイオン、試料イオン。The sample injection chamber (6) forms an interface between the leading liquid and the terminal liquid, and is structured so that the sample can be injected into the interface between the two liquids, and both electrode tanks f31 ( If a high voltage is applied to 41, the leading ion and sample ion.
ターミナルイオンがターミナル電極槽（３）側からリー
ディング電極槽（４）側台こ向がって泳動し。Terminal ions migrate from the terminal electrode tank (3) side to the leading electrode tank (4) side.
移動度の差より分離され、検出器（７１で検出される。They are separated based on the difference in mobility and detected by a detector (71).
泳動管（５）の検出器（７）側に近い部位にはＰＨ測定
用電極（８）が設置されており、泳動する電解液のＰＨ
値を参照電極（９）との電位差として電位差計Ｕ旧こよ
り測定検出し、その測定されるＰＨ値を表示器αυによ
り表示するように構成されている。A pH measuring electrode (8) is installed in the electrophoresis tube (5) near the detector (7) side, and the pH of the electrolyte being electrophoresed is measured.
The pH value is measured and detected by a potentiometer U as a potential difference with a reference electrode (9), and the measured PH value is displayed on a display αυ.
上記のように構成されてなる細管式等速電気泳動装置に
おいて、定電流を通電すると。When a constant current is applied to the capillary isotachophoresis device configured as described above.
分析対象の試料イオンは、リーディングイオンとターミ
ナルイオンに前後をはさまれ泳動が開始される。泳動中
に各イオンは易動度の違いにより９分離され、試料イオ
ンはリーディングイオンの後に↓動度の大きさの順に配
列され、互々は界面で接するゾーンを形成しながらター
ミナルイオンを後続として、各イオンは等速度で泳動管
（５）中を泳動する。電解液のうちリーディング液は、
そのＰＨ値があらかしめわかっているので１表示器（１
１１で表示されるＰＨ値を追跡することにより、ＰＨ値
の変化を読み取ってリーディングイオンに後続する試料
イオンゾーンの先端位置を知ることができる。The sample ions to be analyzed are sandwiched between the leading ion and the terminal ion, and electrophoresis begins. During electrophoresis, each ion is separated into 9 parts due to differences in mobility, and the sample ions are arranged in order of mobility after the leading ion, and the terminal ions follow while forming a zone where they meet each other at the interface. , each ion migrates in the migration tube (5) at a constant velocity. Among the electrolytes, the leading liquid is
Since the pH value is known, there is one display (1
By tracking the pH value displayed at 11, the position of the tip of the sample ion zone following the leading ion can be determined by reading the change in the pH value.
リーディング液のＰＨ値は、たとえば、（０，０１，Ｍ
−塩酸、β−アラニン、０．２％トリトンＸ−１００）
であればＰＨ５，６，（０，０１Ｍ−塩酸、アメジオー
ル、０．２％ＰＶＡ ）であればＰＨ８，８，（０，０
０５Ｍ−ＭＥＳ。The PH value of the leading liquid is, for example, (0,01,M
- Hydrochloric acid, β-alanine, 0.2% Triton X-100)
If PH5.6, (0.01M hydrochloric acid, amediol, 0.2% PVA), PH8.8, (0.0
05M-MES.
１）、 ＯＩ Ｍ−アメジオール、０．１％−ＲＰＣ）
であればＰＨ８，５６，（０，０１Ｍ−水酸化バリウム
、グルタミン、０．２％トリトンＸ−１００）であれば
Ｐ Ｈ１，０，０である。1), OIM-Amediol, 0.1%-RPC)
If it is PH8.56, if it is (0.01M-barium hydroxide, glutamine, 0.2% Triton X-100), it is PH1.0.0.
このようにＰＨ値を測定表示させることにより、試料イ
オンゾーンの先端位置がＰＨ測定用電極（８）に達した
ときをこＰＨ表示値が表化し、先端位置がＰＨ測定用電
極（８）付近に到達したことを知ることができるのであ
るが。By measuring and displaying the PH value in this way, the PH display value is displayed when the tip position of the sample ion zone reaches the PH measurement electrode (8), and the tip position is near the PH measurement electrode (8). However, we can know that we have reached this point.
ＰＨ測定用電極を多数個併設し、各電極位置でのＰＨ値
変化を読み取って先端位置の確認を複数個所で極細かく
行なえるようにしてもよく、泳動状態の表示も電極の個
数と同数個設けるか、１個にして切換表示するようにす
ることもできる。さらに表示器（点滅ランプ式）を多数
設けて、これを順次点灯する方式とすることもできる。A large number of PH measurement electrodes may be installed in parallel, and the change in PH value at each electrode position may be read to allow the tip position to be confirmed in multiple locations in extremely fine detail.The electrophoresis status may also be displayed on the same number of electrodes as the number of electrodes. It is also possible to provide one or only one and switch the display. Furthermore, it is also possible to provide a large number of indicators (flashing lamp type) and turn them on one after another.
またＰＨ測定用電極を、易動度に応じて変化させながら
確認できるようにしてもよく、上記実施例に限定される
ものではない。Further, the PH measurement electrode may be configured to be able to be checked while being changed depending on its mobility, and is not limited to the above embodiment.
（へ）効果
この発明に係る等速電気泳動装置は上記のように構成さ
れ１作動するので、試料分析中の泳動管内の試料イオン
ゾーンの先端位置を容易に知ることができるので、従来
のように分析に先立ちあらかじめ試料イオンの泳動時間
を測定するといった分析操作の二度手間をなくすること
ができ９分析作業能率を高めることができる。(F) Effect Since the isotachophoresis apparatus according to the present invention is constructed and operates as described above, it is possible to easily know the tip position of the sample ion zone in the electrophoresis tube during sample analysis. In addition, it is possible to eliminate the need for double analysis operations such as measuring the migration time of sample ions in advance prior to analysis, thereby increasing analysis work efficiency.
第１図はこの発明の１実施例である等速電気泳動装置の
構成を示す模式図である。
（３）・・・ターミナル電極槽
（４）・・・リーディング電極槽
（５）・・・泳動管 （６）・・・試料注入室（７）・
・・検出器 （８）・・・ＰＨ測定用電極（９）・・・
参照電極 ａα・・・電位差計（１１１・・・表示器
第１図FIG. 1 is a schematic diagram showing the configuration of an isotachophoresis apparatus which is an embodiment of the present invention. (3)...Terminal electrode tank (4)...Leading electrode tank (5)...Migration tube (6)...Sample injection chamber (7)
...Detector (8)...PH measurement electrode (9)...
Reference electrode aα... Potentiometer (111... Display figure 1)
Claims (1)
槽を、他方端側に検出器を介してリーディング電極槽を
それぞれ配設し、これら両電極検量に高電圧を印加する
ことにまって泳動管内蚤こ泳動電流を通電させる等速電
気泳動装置において、前記泳動管路中に電解液のＰＨ値
を測定するためのＰＨ測定手段と、このＰＨ測定手段か
らの出力により泳動状態を表示するための表示手段とを
備えたことを特徴とする等速電気泳動装置。A terminal electrode tank is installed at one end of the electrophoresis tube through a sample injection chamber, and a leading electrode tank is installed at the other end through a detector, and a high voltage is applied to both electrodes for calibration. In an isotachophoresis apparatus in which an electrophoresis current is applied in an electrophoresis tube, a pH measuring means for measuring the pH value of an electrolytic solution is provided in the electrophoresis tube, and an output from the pH measuring means is used to display the electrophoresis state. An isotachophoresis apparatus characterized by comprising: a display means for.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP59061943A JPS60203848A (en) | 1984-03-28 | 1984-03-28 | Isokinetic electrophoresis device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP59061943A JPS60203848A (en) | 1984-03-28 | 1984-03-28 | Isokinetic electrophoresis device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS60203848A true JPS60203848A (en) | 1985-10-15 |
| JPH0552459B2 JPH0552459B2 (en) | 1993-08-05 |
Family
ID=13185773
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP59061943A Granted JPS60203848A (en) | 1984-03-28 | 1984-03-28 | Isokinetic electrophoresis device |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS60203848A (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0573378U (en) * | 1992-03-13 | 1993-10-08 | 日信工業株式会社 | Solenoid valve device |
| JPH0573377U (en) * | 1992-03-13 | 1993-10-08 | 日信工業株式会社 | Solenoid valve device |
-
1984
- 1984-03-28 JP JP59061943A patent/JPS60203848A/en active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0552459B2 (en) | 1993-08-05 |
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