JPS6033400Y2 - Particle aggregation determination container - Google Patents

Description

[Detailed description of the invention] The present invention relates to a particle agglutination determination container used for determining agglutination patterns by immunological agglutination reactions, and in particular, it is used for determining various blood types and detecting antibodies and antigens from agglutination patterns of blood cell particles. It relates to a container for carrying out.

For example, in the conventional method for determining blood type, a reaction container with a curved bottom shaped like a wine cup is used, and a 2 to 5% suspension of test blood cells obtained by centrifugation and a specific antiserum are placed in this container. Dispense a fixed amount of blood, stir both, leave to stand, then perform centrifugation, vibrate the reaction vessel vigorously to shake out the precipitated blood cells, and then vibrate relatively slowly to remove the aggregated components from the vessel. Some types form an agglomerated pattern by concentrating it in the center of the bottom surface, and detect this photometrically.

This blood type determination method involves vigorously shaking the reaction container after centrifugation to separate the precipitated blood cells from the bottom of the container, and is therefore used to determine the ABO blood type, which has a strong cohesive bond.

However, in the case of immunological agglutination reactions with weak binding strength, such as when determining the RH blood type, or when detecting various irregular antibodies, antigens, HBs antigens, etc., the above-mentioned determination is necessary. method is not available.

That is, if the cohesive bonding force is weak, particles such as blood cells that are once bound will separate when the reaction container is vibrated, and will not collect in the center of the reaction container.

On the other hand, in Japanese Patent Application No. 54-77643, the applicant has developed a container for determining particle aggregation, which is capable of clearly forming an aggregation pattern by natural settling, regardless of the strength of the aggregation bond, by providing regular steps. Proposed.

In such a container, the transmittance of the lowest part of the bottom and the periphery can be photoelectrically measured, and based on the difference between the two, it is possible to determine whether aggregation or non-aggregation occurs.

However, in the case of this container, if the bottom of the container is made thin and small at the bottom like a cone, it is possible to clearly form agglomerated and non-agglomerated patterns, but it is not always easy to set criteria for discrimination.

This is because in the case of non-agglomeration, particles that are not aggregated and bonded roll to the lowest part of the bottom of the test container and slide down, forming a non-agglomerated pattern as the non-agglomerated particles accumulate at the lowest part of the bottom. In the case of a determination container with a shaped bottom surface, the lowest part of the bottom surface is sharp, so non-agglomerated particles tend to accumulate on the bottom surface, and the test liquid causes an agglutination reaction, causing most of the particles to aggregate and accumulate on the slanted bottom surface. Even in the case where there are some particles that do not participate in the agglomeration bond for some reason, these particles accumulate at the bottom of the conical bottom of the judgment container, forming a small but accumulated pattern. This is because it may be mistaken for non-aggregation.

The purpose of the present invention is to solve the above-mentioned various problems, and to make it possible to clearly form an agglutination pattern by immunological agglutination reaction regardless of the strength of agglutination binding force, and to make it possible to clearly distinguish between agglutination and non-aggregation. The purpose is to provide a container for determining particle aggregation that is appropriately constructed for the purpose of the present invention.

In order to achieve this objective distantly, the particle agglutination determination container of the present invention has at least a portion of the bottom surface inclined, and has a test liquid storage portion in which a plurality of steps are regulated on at least a portion of the slope. In a particle agglutination determination container for performing immunological analysis by storing a test solution containing particles in the storage part in a substantially stationary state and determining an agglutination pattern formed on the bottom surface due to natural sedimentation of the particles. , characterized in that the lowermost part of the bottom surface is substantially flat.

The present invention will be described in detail below with reference to the drawings.

FIG. 1 is a cross-sectional perspective view showing the structure of an example of a container for determining particle aggregation according to the present invention.

In the particle aggregation determination container 1 of this example, the bottom surface of the test liquid storage part is a conical sloped bottom surface, and the lowest part 2 of the bottom surface is configured to be flat as if the apex of the conical shape was cut off.

The conical sloped bottom surface has a plurality of steps 3 concentrically and regularly arranged around the center of the lowest part 2, and the cross-sectional shape of the sloped surface of the sloped bottom surface is configured in a sawtooth shape. It is.

In this example, the inner diameter of the container 1 is 61rIM1, and the height H of the inclined bottom surface is 1.5rIr! n, and the angle of inclination of the inclined bottom surface with respect to the horizontal was approximately 30°.

Further, the depth h and the length 1 in the inclination direction of each step were set to 2 to 50 μm and 5 to 200 μm, respectively.

The diameter d of the lowest part of the bottom was 0.5 mm.

This diameter d needs to be changed depending on the absolute amount of reactive blood cells, and is between 0.1 and 1. The range of orryL is suitable for the above purpose.

In addition, this container can be integrally formed from a chemical-resistant plastic material, and in order to be used for a large number of test items, a plurality of test liquid storage parts 6 are added to the plate-shaped judgment container 5 as shown in FIG. The format provided is also effective.

Since the particle aggregation determination container of the present invention is constructed in this way, a clear aggregation pattern can be formed regardless of the strength of the aggregation bonding force of the particles, and it is possible to clearly determine whether the particles are agglomerated or not.

Note that the particle aggregation determination container of the present invention is not limited to the illustrated embodiment, and various modifications can be made within the scope of the utility model registration claims.

For example, the shape of the sample liquid storage portion of the determination container is not limited to a conical shape, but may also be a semi-conical shape with one side vertical, a triangular pyramid, or a quadrangular pyramid shape.

Furthermore, the step provided on the slope is not limited to a step-like one, but may be formed by forming a convex portion or a recess on the slope, and these steps may be divided and provided on the circumference. is also possible.

[Brief explanation of the drawing]

FIG. 1 is a cross-sectional oblique view showing the structure of an example of the container for determining particle aggregation of the present invention, and FIG. 2 is a perspective view showing another embodiment of the container for determining particle aggregation of the present invention. 1... Container for determining particle aggregation, 2... Bottom, 3... Step, 5... Container for determining particle aggregation, 6... Test solution storage part, D...Inner diameter, H...Height of the inclined bottom, h...Height of the step, 1...Length in the inclined direction S-d...
- Bottom diameter.

Claims (1)

[Scope of utility model registration request] At least a part of the bottom surface is an inclined surface, and the test liquid storage part has a plurality of steps regularly provided on at least a part of the inclined surface, and the test liquid containing particles is kept in a substantially stationary state in the storage part. In a particle aggregation determination container for performing immunological analysis by determining an agglutination pattern formed on the bottom surface due to natural sedimentation of the particles contained in the container, the lowermost part of the bottom surface is configured to be substantially flat. Characteristic particle aggregation determination container.