JPS6110209Y2 - - Google Patents

Description

[Detailed description of the invention] The present invention has a large number of dispensing needles arranged in the front and back and left and right directions, mounted on the machine with the needle tips facing downward, and below the dispensing needles. A plate stand is provided so as to be reciprocated between a position immediately below the dispensing needles and a position laterally removed by an actuating device, and the relative vertical distance between the plate stand and the tip of the dispensing needle is determined. This invention relates to an improvement in a dispensing device that is freely movable using a separate support frame.

The above-mentioned dispensing device moves the plate stand on which the test tube is placed to a position directly below the dispensing needle, and from that state closes the vertical relative distance between the plate stand and the tip of the dispensing needle, and then removes the test tube. When performing dispensing work with the dispensing needle inserted into the dispensing needle, a bead of dispensing liquid may remain on the tip of the dispensing needle, resulting in inaccurate dispensing volume. In addition, when the antibody-attached beads in the test tube are washed with distilled water dispensed from the dispensing needle during the testing process, the dispensed distilled water hits the beads directly, causing damage to the areas where they hit. There is a problem that the test becomes inaccurate due to local cleaning.

The present invention was made to solve this problem, and the plate stand moved to a position directly below the dispensing needle by an actuating device is moved between the plate stand and the tip of the dispensing needle by another actuating device. The operation of inserting the needle tip of the dispensing needle into the test tube placed on the plate stand by reducing the relative vertical distance between the test tube and the dispensing needle causes interference between the test tube and the dispensing needle. When dispensing the liquid from the dispensing needle into the test tube after completing the operation, make sure that the dispensing needle is biased to one side of the test tube and close to the inner wall. The purpose is to provide a dispensing device that is

For this purpose, in this invention, the aircraft A
A large number of dispensing needles 13 with their needle tips facing downward are arranged at a constant pitch, and below the dispensing needles 13, an actuator is used to operate the dispensing needles 13. A plate stand 5 is provided so as to reciprocate between a position immediately below and a position off to the side, and the relative distance in the vertical direction between the plate stand 5 and the tips of the dispensing needles 13 is controlled by different operations. In the dispensing device which can be freely varied by the device, the plate stand 5 is positioned at the second stroke end of the forward stroke toward the position directly below the dispensing needle 13 by the actuating device.
From that position, the dispensing needle 13...
The machine body A is equipped with a control switch 9 that operates the actuating device to slightly move the plate table 5 laterally when it reaches the stroke end of the operation to shorten the vertical relative distance to the needle tip. The present invention provides a dispensing device with characteristics.

Next, an example of implementation will be described in detail with reference to the drawings.

FIG. 1 is a front view of a dispensing device in which the present invention can be implemented. In the figure, A indicates the body of the dispensing device, and 1 indicates a pump device for suction and discharge provided in the body A.

As shown in FIG. 2, the pump device 1 includes a cylinder 11 supported by a pump main frame 10 and a piston 12 slidably fitted into the cylinder 11. The pump main frame 10 is attached to a support frame 2 (a third
(Figure) shows the mounting support. Referring again to FIG. 2, a large number of cylinders 11 of the pump device 1 are attached to the pump main body frame 10 in a manner that they hang down from the pump main body frame 10. Further, a dispensing needle 13 shaped like a syringe needle is attached to the lower end of each cylinder 11, and a piston 12 is fitted into each cylinder 11, so that each cylinder 11 constitutes an independent pump device 1. are doing. The upper end of each piston 12 is connected and supported by a plate 15 which is supported by a guide post 10a provided on the upper surface side of the pump body frame 10 so as to be able to rise and fall freely with respect to the pump body frame 10. , the plate 15 is the spring 14
As a result, the pistons 12... rise all at once as shown in Fig. 2 to perform a suction operation, and by pushing down the plate 15 against the bias of the spring 14, the pistons 12... is lowered to perform the dispensing (discharging) operation.

In addition, in this embodiment, each pump device 1 is configured to aspirate and dispense a minimum amount from the dispensing needle 13 at one time.
The diameter of the cylinder 11 and the stroke of the piston 12 are set to 0.1 milliliter and a maximum of 3 milliliter. The pump device 1 consisting of the cylinder 11 and the piston 12 is arranged so as to correspond to each test tube 40 arranged in the test tube stand 4 (FIG. 1)...or each test hole (not shown) in the titration plate. There are 50 pieces arranged vertically and horizontally.

In FIG. 3, numeral 3 indicates a pressing member that presses the plate 15. As shown in FIG. The pressing member 3 includes a piston pressing plate 31 connected to the upper surface of the plate 15.
The lower end sides of the threaded rods 33 are respectively screwed into the four screw cylinders 32 provided at the four corners of the upper surface for four-point support, and the upper end portions of the threaded rods 33 are attached to the support frame 2. Sprockets 34 are supported by rotation and project upward, and are attached to the respective projecting parts.
... and the sprocket 35 of the piston push plate driving motor M1 disposed on the side of the support frame 2,
The conduction chain 36 is wound around, and the threaded rods 33 are rotated clockwise and counterclockwise by the forward and reverse rotation of the motor M1, thereby raising and lowering the piston pusher plate 31, thereby raising and lowering the plate 15. There is. In addition, the piston push plate driving motor M1
is made for low speed rotation, and the above motor M
1 sprocket 35 and the shaft 3 protruding on the opposite side
A disc 37 is attached to the disc 37, and a cutout hole 38 is provided on the outer periphery of the disc 37 so that the outside is open.
Switches (phototubes) S and S for counting the number of notch holes 38 are arranged above and below the rotation locus of the notch holes 38, and the switches S and S are set by the rotation of the disc 37. The cutout hole 38 that traverses
The number of revolutions of the motor M1 is controlled by the number of digital control boards 17 (first
When the dispensing amount shown in FIG. 12 is adjusted to 2 milliliters using the knob 18, for example, the piston 12 slowly moves up and down by the amount of 2 milliliters.

Referring again to FIG. 1, 7... is a suction needle that is suctioned by a pump device 73 (FIG. 3) dedicated to suction;
It is shaped like a normal dispensing needle, with 50 needles arranged vertically and horizontally so that the tips point downward. Each suction needle 7 has its proximal end protruding into the chamber 71 from the bottom plate member 72 of the chamber 71 whose circumferences are sealed as shown in FIG. The chamber 71 is suspended from the bottom plate member 7.
The connecting port 72a provided in the chamber 72 is connected and communicated with the suction port 73a of the pump device 73 via a pipe 74, and the air in the chamber 71 is extracted by the suction operation of the pump device 73. Each suction needle 7
... are designed to perform the suction operation all at once.

5 indicates the lower part of the suction needle 7 and the dispensing needle 13.
The plate stand 6 is mounted on the machine stand 19 so as to face the lower part of the dispensing needles 13.... A liquid dish 60 is placed on the pedestal 6, and a test tube stand 4 in which test tubes 40 are lined up or a titration plate (not shown) is placed on the plate stand 5. Then, the plate stand 5 moves from the position below the aspiration needle 7 to the position below the dispensing needle 13 and then reciprocates from side to side so as to return to the position below the aspiration needle 7. It reciprocates up and down to move between a position where the lower end side of the dispensing needle 13 is inserted into the liquid dish 60 placed on the upper surface and a lowered position where it is retracted below the movement trajectory of the plate stand 5. As a result, a predetermined amount of liquid is sucked from the liquid tray 60 of the pedestal 6 by the dispensing needle 13.
Instead of the liquid dish 60 on the pedestal 6, the liquid is dispensed into each test tube 40 on the plate pedestal 5, which moves below the dispensing needle 13, and the plate pedestal 5 returns to its original position. As a result, the dispensed liquid in the test tube 40 is aspirated (washed) by the suction needle 7.
In this case, the above-mentioned suction (washing) refers to the test tube 40.
It does not mean "washing", but refers to the action of sucking out all the liquid used in the previous step during the inspection process.

In addition, when aspirating and dispensing, the aspiration needle 7... side and the dispensing needle 13... side may move up and down with respect to the plate stand 5 and pedestal 6, or vice versa. may be moved up and down, and may be implemented as appropriate.

In this embodiment, as shown in FIGS. 3 and 4, the device slides left and right along two rails 51, 51 provided across the body A of the dispensing device to the left and right. The trolley 52 is supported, and the trolley 5
The plate stand 5 is supported by a vertical rod 53 provided on the plate stand 5 so that it can be raised and lowered, and the said dolly 52 is controlled to reciprocate from side to side by an actuating device, and the rod 53 supporting the plate stand 5 is actuated. It is controlled by a device to move up and down.

The actuating device that reciprocates the cart 52 left and right is a drive pulley 54 that moves forward and backward (indicated by arrow A in FIG. 3) by a motor M2 attached to the body A of the dispensing device, and a drive pulley 54 on the left and right sides of the rails 51, 51. The motor M
2, the carriage 52 is configured to reciprocate from side to side.

Further, the actuating device is as shown in FIG.
A motor M3 is attached to the underframe 52a of the truck 52, and a gear 57 is provided on the rotating shaft of the motor M3.
The rack 58 in the vertical direction that engages with the underframe 52
Supported so that it can be raised and lowered freely on a guide 59 that is erected at a.
Connect the rack 58 to the plate stand 5,
The plate stand 5 is moved up and down by forward and reverse rotation (arrow RO) of the gear 57. The positioning of each stroke end of the vertical movement of the plate stand 5 and the left and right reciprocating movement of the trolley 52 is performed by micro switches S1 and S disposed at both the left and right ends of each rail 51.
This is accomplished by controlling the motors M2 and M3 on and off using micro switches S3 and S4 provided at both upper and lower ends of a guide 59 that supports the rack 58.

In addition, the pedestal 6 is normally attached to the rail 5.
It is moving downward to be located below 1.51,
It is moved up and down by the actuating device. As shown in FIG. 3, the actuating device has a vertical rack 62 that meshes with a gear 61 of a motor M4 attached to the body A of the dispensing device supported by a vertical guide 63 provided on the body, By connecting the rack 62 to the pedestal 6, the rack 62 is configured to reciprocate up and down (arrow C) by forward and reverse rotation of the gear 61, and the upward and downward strokes of the pedestal 6 are controlled. The end positioning is performed by controlling the motor M4 on and off using microswitches S5 and S6 provided at both the upper and lower end portions of the guide 63. The micro switches S3 and S5 contact the rising plate pedestal 5 and pedestal 6, and issue a signal to stop the operation of the motors M3 and M4, while at the same time operating the pump devices 1 and 73 in a timely manner. It is electrically wired to the circuit of the drive motor of the device 1,73.

In FIG. 1, 8... controls the microswitches S1, S2, S3, S4, S5, and S6 to operate in a well-ordered manner in accordance with the movement of the plate 5 and the pedestal 6, so that the liquid flows as shown in FIG. plate 60
Dispense the aspirated liquid into each test tube 40,
These control buttons are used to perform switching control such as repeating the operation of suctioning (cleaning) the liquid with the suction needle 7, or repeating the operation of only suction and dispensing as shown in FIG. 5.

9 reaches the stroke end of the forward stroke in which the plate stand 5 is moved by the actuator to a position directly below the dispensing needles 13, and from that position the actuator moves the tip of the dispensing needles 13 upward and downward. This is a control switch that operates the actuating device to move the plate stand 5 by a slight amount laterally when the stroke end of the movement is reached when performing a lifting operation to shorten the relative distance. 5 is installed in the fuselage A in such a way that it contacts the plate stand 5 at the same time when it comes into contact with the micro switch S5 to stop the lifting operation.

The amount of operation of the actuator by this control switch 9 is such that the tip of the dispensing needle 13 located at the axial center of each test tube 40 as shown in FIG. 40... Each test tube 40... is biased to one side within...
The amount is extremely small until it comes into contact with the inner wall of the

The dispensing device according to the present invention configured as described above is configured to control the stroke end of the operation when the plate stand 5 carrying the test tubes 40 is moved to a position directly below the dispensing needles 13 by the operation of the actuating device. , the tip of the dispensing needle 13 may be set above the axial center position of the test tube 40, so that the tip of the dispensing needle 13 is placed inside the test tube 40 by actuating the actuator at that position. The operation of reducing the vertical relative distance between the pedestal 5 and the tip of the dispensing needle 13 so that the test tube 40 is inserted into the dispensing needle 13 prevents interference between the test tube 40 and the dispensing needle 13. Now you can do it smoothly,
Moreover, when the operation of reducing the vertical relative distance between the pedestal 5 and the tip of the dispensing needle 13 reaches the stroke end in the above-mentioned state, the test tube 40 is automatically moved to the tip of the dispensing needle 13. The tip of the dispensing needle 13 approaches the inner surface of the tube wall of the test tube 40, so that the amount that remains on the tip of the dispensing needle 13 when dispensing is finished. The droplets of the injected liquid will surely drip down the inner surface of the tube wall, making the dispensed amount accurate. In addition, when a washing liquid such as distilled water is dispensed from the dispensing needle 13 into the test tube 40 to wash the antibody-attached beads 44 placed at the bottom of the test tube 40, the dispensed liquid is dispensed. Since the cleaning liquid flows down the inner surface of the tube wall of the test tube 40, uneven cleaning caused by direct contact of the cleaning liquid with a localized portion of the surface of the antibody-attached beads 44 can be effectively prevented.

[Brief explanation of the drawing]

Figure 1 is a front view of the dispensing device implementing the present invention.
Figure 2 is a partially cutaway front view of the pump device of the same device, Figure 3 is an overall schematic explanatory diagram of the same device, and Figure 4
The figure is a schematic view of the cradle of the same device as seen from the side.
The figure is an explanatory diagram showing the movement of the pedestal and setting base of the same device as above, Figure 6 is an explanatory diagram showing the relative position of the conventional dispensing needle and test tube, and Figure 7 is the dispensing needle of the device of the present invention. FIG. 3 is an explanatory diagram showing the relative positions of the test tube and the test tube. Explanation of drawing symbols, A... Aircraft, M1 to M6...
Drive motor, S... switch, S1 to S6...
...Micro switch, 1...Pump device, 10...
...Pump main frame, 10a...Guide post, 11
... Cylinder, 12 ... Piston, 13 ... Dispensing needle, 14 ... Spring, 15 ... Plate, 16 ...
...Panel, 17...Control board, 18...Knob, 1
9... Machine base, 2... Support frame, 3... Pressing member,
31... Push plate, 32... Threaded tube, 33... Threaded rod, 34, 35... Sprocket, 36... Conduction chain, 37... Disc, 38... Notch hole, 39
...Axis, 4...Test tube stand, 40...Test tube, 44
...Antibody-attached beads, 5...Plate stand, 51...
...Rail, 52...Dolly, 52a...Underframe, 53
... Rod, 54 ... Pulley, 56 ... Transmission belt, 57 ... Gear, 58 ... Rack, 59 ... Guide, 6 ... Pedestal, 60 ... Liquid plate, 61 ... Gear, 62 ... Rack , 62a... underframe, 63...
Guide, 7... Suction needle, 71... Room, 72...
Floor plate member, 72a... Connection port, 73... Pump device, 73a... Suction port, 74... Pipe, 8...
Control button, 9... Control switch, ,,... Actuation device.

Claims (1)

[Scope of utility model registration request] A large number of dispensing needles 13 with the needle tips facing downward are arranged in a fixed pitch on the machine A, and below the dispensing needles 13, an actuator is used to move the dispensing needles 13 downward. A plate stand 5 is provided so as to be able to reciprocate between a position directly below and a position laterally removed, and the plate stand 5 and the dispensing needle 13
In a dispensing device in which the relative distance in the vertical direction between the dispensing needle 13 and the tip of the dispensing needle 13 can be varied by another actuating device, the plate stand is positioned at the end of the stroke of the reciprocating stroke of the dispensing needle 13 toward the position directly below the dispensing needle 13 by the actuating device. 5 reaches the stroke end of the operation of reducing the vertical relative distance between the dispensing needle 13 and the tip of the dispensing needle 13 by another actuating device from that position, and the aforementioned actuating device moves the plate stand 5 slightly laterally. A dispensing device characterized in that a control switch 9 for operating the machine body A is installed.