JPS6112601A - Sex attractant for melanotus okinawensis ohira - Google Patents

Abstract

PURPOSE:The titled sex attractant, containing dodecyl acetate as an active constituent, and capable of controlling injurious insects in an imago period as an object by attracting and killing a large number of males or disturbing the copulation behavior of females and males. CONSTITUTION:A sex attractant for Melanotus okinawensius Ohira containing dodecyl acetate which is an attractant substance released from virgin female of Melanotus okinawensis Ohira and expressed by the formula as an active constituent. The attractant is used by adsorbing the above-mentioned active constituent in an amount of 1-1,000mg based on 1g carrier on a suitable carrier, or sealing the active constituent in a molded article of a carrier material. The attractant is capable of attracting many male imagoes and a small number of female imagoes with an attractive force equal to that of the virgin female.

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a sex attractant for the Japanese white-spotted cricket.

Melanotus
okinawensis 0hira) is an important pest of sugarcane. The larvae feed on sugarcane buds, root bands, and stems underground, resulting in missing plants in sugarcane seedlings, withering or severely stunted growth in growing stems, and damage caused by feeding damage on harvested stems. Red rot causes a decline in quality and yield. In addition, this species is a type of wire beetle that has been cited as one of the causes of sugarcane failure to sprout, which has been a problem in sugarcane cultivation in Okinawa Prefecture for the past 10 years. For control, chemical treatment has sometimes been used during planting, but
Because eggs are laid by adults that emerge in early spring, no fundamental control method has been established. Chemical control methods for adult and growing sugarcane are being considered, but since it is a soil pest, it is difficult to predict the density of infestation, and it is important to accurately understand the amount of adult infestations in early spring.

On the other hand, the chemical structures of so-called B sex pheromones have recently been clarified for many pest insects, and this attracting sex pheromone can now be used to efficiently investigate the development and development of pest insects. A sex pheromone is generally a chemical substance secreted by female adult Ji, which exhibits a species-specific attracting effect on male adults of the same species. By clarifying the chemical structure of such attractive sex pheromones and using them as so-called sex attractants, it becomes possible to efficiently investigate their development and development. Furthermore, it is also possible to control pests targeting the adult stage by using this sex attractant to attract and kill a large number of males or to disrupt the mating behavior of males and females.

Under these circumstances, the results of a field experiment using virgin females of the Okinawa-kanshikushikometsuki as an attraction source revealed that the Xiong adults attract a large number of male adults and a small number of female adults.

Akira Kino and his colleagues conducted research on the substances released from these virgin females, extracted the components that have attracting activity, and determined their chemical structures. Furthermore, we learned that the compound exhibits an effective attracting effect on male and female adults of Okinawakanshakushikoplating. The present invention was completed based on these findings.

The compound according to the present invention has a structural formula: %Formula% When the above-mentioned compound according to the present invention is used as an active ingredient of an attractant for Okinawakanshakushiko plating, various usage forms are possible.

This metal compound is adsorbed onto a suitable carrier (such as various synthetic polymers, natural rubber, etc.), or encapsulated in a molded product of these carrier materials, and used in the form of a string, cylinder, or sphere.

The content of the active ingredient can be determined as appropriate, but when adsorbed to a carrier or encapsulated in a molded carrier material,
Approximately 1 to 1000 μg/g of carrier is preferable.

The carrier or carrier material molded product containing the active ingredient is placed on a suitable support, for example, on a container (such as a vat) containing water or other liquid, or on an object coated with a suitable adhesive substance. Or, by placing it near the container, it attracts the Okinawa Kashikushikmetsuki, which falls into the container (such as a hat) or gets caught in the sticky substance and dies.

The present invention will be described in detail below.

In order to elucidate the chemical structure of the attractant of the Okinawa-kanshakushikmetsuki, we first collected the attractant active substance emitted by the virgin female using a mouthpiece.

The presence or absence of activity was determined using the following assay method. A glass cylinder with a diameter of 8 cm and a length of 160 cm is used, and a speed of 40 c per second is used inside it.
A wind of m flowed. The sample was placed on the windward side and the male adult was released from the leeward side. When active, the male adult exhibits exciting behavior such as protruding copulatory organs, moving around, and flying.
This was determined by being attracted to the sample. The paper that had adsorbed the released material from about 2,500 virgins was washed with hexane, concentrated, and then fractionated using a column packed with Florisil. The active fraction was eluted with 5% ether/hexane.

Further, both fractions were purified using a column packed with silver nitrate silica gel, and the active fraction was eluted with 3% ether/petroleum ether. Next, the active fraction was purified by gas chromatography. When a 3% oV-101 column was used, activity was observed at a relative retention time of 0.83 to 1.18 with dodecyl acetate. When this portion was further purified using a 10% PEG-20M column, activity was observed only in a peak with a relative retention time of 0.92 to 1.0.

Next, the following analysis was performed to determine the chemical structure of this peak. When analyzed using a gas chromatograph mass spectrometer (equipment: JEOLD-300, column: 10% PE
G 20M200℃, ionization voltage near 0eV'),
The spectrum matched that of an acetate ester of a saturated linear alcohol with 12 carbon atoms. The first mass spectrometry spectrum
As shown in the figure.

From the above, the attractant for Okinawakan shakushiko plating is a compound with the following chemical structure, CH3 (CHz) +
It was concluded that +0CCHa.

Next, we compared the attraction activity of virgin females with a composite of ingredients that had been shown to have this activity in the field. Using a U-shaped adhesive 1-wrap, the attraction activity between a rubber cap impregnated with 1 to 10 mg of the synthetic product and virgin females was compared. The result is the first
Shown in the table.

Table 1 Dodecyl acetate synthetic product and attraction activity of virgin females under field conditions Dodecyl acetate Total number of animals attracted and killed - 1 mg 80 5 mg 351 10 mg 417 Virgin #4 313 (Note) Attraction period from March 19th From April 20th, it was revealed that a rubber cap impregnated with 5 mg of dodecyl acetate synthetic product has a strong attracting force comparable to that of four virgin females. In addition, the ratios of male and female individuals lured and killed by traps using virgin females and synthetic products as attraction sources were 95.5-4.5 and 95.8-4.2, which were almost the same for both.

[Brief explanation of drawings]

FIG. 1 is a mass spectrometry spectrum of a substance separated from vIL from virgin release material of Okinawakanshakushiko plating.

Claims (1)

[Claims] Okinawakan consisting of dodecyl acetate A sexual attractant for white-crowned crickets.