JPS61218501A - Apparatus for internal organ perfusion - Google Patents

Abstract

PURPOSE:The titled apparatus, having a liquidtight container with an air vent in the upper part for filling a perfusate and further netlike holding member for holding an internal organ in the central part of the above-mentioned container, having high thermostatic efficiency and capable of holding the internal organ without damaging the organ. CONSTITUTION:An apparatus having a preservation container 30 constituted of a container body 31 and a lid body 33 screwed liquidtight thereto by an O-ring 32 and further a netlike holding member 36 for holding an internal organ 7 almost horizontally in the central part of the body 31. The internal organ 7 is contained in a receiving part 37 of the holding member 36 and a pump 15 is slowly rotated while filling a perfusate from a perfusate filling and discharging port 34 therein to carry out the priming of a perfusion circuit 41. When the perfusate reaches the intermediate point of the receiving bottom of the body 31 and the receiving part 37, a blood vessel of the internal organ 7 is connected to an inlet 42 with a cannula 20. A lid part 38 is mounted, and a lid body is screwed to the container body 31. The perfusate is then filled in the container body 31 while venting air through an air vent (33a). The pump 15 is then rotated at an ordinary number of revolutions to flow the perfusate through a filter 14, the pump 15, heat exchanger 16 and artificial lung 43.

Description

DETAILED DESCRIPTION OF THE INVENTION "Field of Industrial Application" The present invention relates to an organ perfusion device that can preserve organs such as kidneys for transplantation in a fresh state for a predetermined period of time and that can be easily transported by deer.

"Prior Art" Conventionally, as an organ perfusion device, one having the structure shown in FIG. 4 is known. Reference numeral 1 in the figure indicates a storage container. The storage container 1 is composed of a container body 2 and a lid 3 detachably attached to the upper part of the container body 2.

The container body 2 is divided into two upper and lower chambers by a partition wall 4. A perforated plate 6 is horizontally provided approximately in the center of the upper chamber 5, and the organ 7 for preservation is placed on this perforated plate 6. The lower chamber 8 is for storing a required amount of perfusate (component similar to plasma) 9, and a perfusate outlet 10 and a perfusion outlet 11 are formed at the bottom. One end (C suction port) of a perfusion circuit 12 is connected to the perfusion outlet 11, and the other end C (outlet) of this perfusion circuit 12 is connected to the perfusion liquid inlet formed on the upper side of the container body 2. 13, this perfusion circuit 12 is equipped with a filter 14, a perfusion pump 15, a heat exchanger 16, a total of 17, and a pressure/temperature monitor 18 in order from the outlet 11 to the inlet 13. . The heat exchanger 1
6, a heat exchange liquid at a constant temperature of about 4° C. is supplied from a constant temperature bath 19. A cannula 20 connected to the organ 7 is connected to the injection port 13 . Therefore, the perfusion fluid supplied to the organ 7 by the perfusion circuit 12 is discharged from the organ 7 and passes through the perforated plate 6.
It accumulates at the bottom (partition wall) of the upper chamber 5. The partition wall 4 has a flow outlet 4a,
4b is formed, and this flow port 4a * 4b can be introduced into the oxygenator 21 provided at the upper part of the lower chamber 8,
The fluid is oxygenated by the artificial lung 21 and is temporarily stored at the bottom of the lower chamber 8.

"Problems to be Solved by the Invention" By the way, the conventional organ perfusion device described above has the following problems, and it is desired to solve these problems.

(b) As mentioned above, the optimum temperature for preserving organ 7 is C4°C.
This is done by perfusing the organ 7 with 1 lll of perfusate that has been adjusted to around 94°C using the heat exchanger 16, but with this method, heat dissipation from the protective surface (C) During transportation, the perfusate 9 in the lower chamber 8 of the container 1 bubbles due to its nature due to vibration, and the bubbles are perfused into the organ 7 via the perfusion circuit 12. blood vessels may become occluded.

(c) During storage, (the part of the container 7 that contacts the perforated plate 6 is the organ 7
damage due to its own weight.

B) For the same reason as above, the igniter 7 is susceptible to vibrations during transportation and is easily damaged.

(e) The storage container 1 has a double structure having an upper chamber 5 and a lower chamber 8t, and requires a complicated mold, which increases the molding cost and makes it impossible to reduce the structural cost.

"Means for Solving the Problems" The organ perfusion system Wt of the present invention eliminates the partition wall of the storage container, and interposes the internal oxygenator in the external perfusion circuit to make the storage container liquid-tight. The storage container has a structure with an air vent at the top, and a net-like holding member for holding the organs 11 in the slurry almost horizontally in the center of the storage container.

"Function" According to the above configuration, it is possible to fill the entire space of the container with the perfusion liquid and perfuse the organ with the organ immersed in the perfusion liquid. (a) Perfuse the inside and outside of the organ. A liquid can be present, and the constant temperature efficiency can be increased.

(b)! The flowing liquid fills the entire space of the container and there is no air in the container, so it does not bubble even when subjected to vibrations, and there is no risk of occluding blood vessels in organs.

(C) @ The device is suspended in the perfusion fluid, and is held by a net-like holding member that has a small contact area and contacts uniformly, so it is not susceptible to damage due to its own weight and vibration. Gui.

(+1) Since the container can have a t-2 layered structure, the mold cost is low and manufacturing costs can be reduced.

Hereinafter, this invention will be explained in more detail with reference to Examples.

"1! Ume example" Figure g1 shows an actual example of this invention 4, so I! 4
The same reference numerals are given to parts common to those in the figures to simplify the explanation. In the figure, reference numeral 30 indicates a storage container, and this storage container 30 is composed of a container body 31 and a lid 33 that is liquid-tightly screwed onto the container body 30 with an O-ring 32. A irrigation fluid filling and discharge port 34 is formed at the lower end and side of the container body 31, and an irrigation outlet 35 is formed at the other side of the lower end. A net-like holding member 36 for holding the organ 7 substantially horizontally is provided in the section. This net-like holding member 3
6 is composed of a receiving part 37 and a lid part 38, as shown in FIGS. 2 and 3. The receiving portion 37 and the lid portion 38 are made of, for example, plastic and are formed into a net shape and a cap shape, and seven flanges 39 and 40 for attachment are formed on the outer periphery of each. For example, the receiving portion 37 is fixed to the container body 31.

Hooks 39a and 39b are provided on the side of the brim-like flange 39 of the receiving portion 37 facing the lid portion 38, and the hook 39a is provided on the brim-like flange 40 of the lid portion 38.

391) are formed into fitting holes 40a and 40b. Therefore, by connecting the lid part 38 to the receiving part 37, the organ 7 can be held inside. Further, above the mesh holding member 36 on the side of the container body 31, a cannula 20 connected to the organ 7 and a perfusion circuit 4 are provided.
A perfusion inlet 42 is formed to connect the two channels, and the perfusion circuit 41 has a filter 14 and a flow pump 15 sequentially connected from the outlet 35 to the inlet 42.
, a heat exchanger 16 and an oxygenator 43 are interposed, and a pressure gauge 44 is installed between the oxygenator 43 and the injection port 42 .

On the other hand, the lid body 33 has a conical shape, as shown in FIG.

In FIG. 1, the reference numeral 46 indicates a thermometer. The organ perfusion device having the above structure is used in the following manner.

Fil The lid 33 is removed and the organ 7 is placed in the receiving part 37 of the mesh holding member 36.

(2) While filling the container 3o with perfusion liquid from the filling and discharge port 34, slowly turn the perfusion pump 15 to prime the a flow circuit 41.

(3) After priming the perfusate to the middle of the bottom of the container body 31 and the receiving part 37, connect the blood vessel of the organ 7 and the injection port 42 with the cannula 20, attach and fix the lid part 38, and close the lid body 33. Screw on.

(4) While removing the air from the upper part of the lid body 33 through the air vent 33L, purify the perfusion liquid through the filling port/discharge port 34. When filling is completed, attach the cap 45 and at the same time close the filling port/discharge port 34. close.

+51 Rotate the i'tl flow pump 15 at the normal rotation speed,
Perfusion is started. This organ perfusion device provides the following effects.

(1) Organs? while being immersed in fi perfusate;
Since the perfusion fluid is also perfused into the blood vessels, the temperature of the organ 7 can be easily and reliably kept constant and uniform.

(11) Since the entire space VC in the container 30 is filled with perfusion fluid and no air is present, the perfusion fluid does not bubble and will not occlude the blood vessels of the organ 7.

Qll) Since the organ 7 is stored in a suspended state in the perfusate, damage to the organ 7 due to its own weight can be prevented. Since the container 30 is surrounded all around by the net-like holding member 36, the organ will not be damaged even if the container 30 is subjected to vibration during transportation.

(V) The container 30 has a simple structure of a container body 31 and a lid 33.
The mold cost is low because it consists of
Moreover, molding becomes easy, and manufacturing costs can be reduced.

In the above embodiment, the lid 33 is conical, but the shape is not limited to a conical VC (other shapes may be adopted as long as air can be easily evacuated). AAlso, although the lid 33 is attached to the container body 31 by screwing, it is possible to maintain airtightness and liquidtightness.
Other attachment means may be used as long as they are removable.

"Effects of the Invention" As explained above, according to the organ perfusion device according to the present invention, it is possible to easily and reliably maintain the appropriate temperature for organ preservation, and the organ perfusion device can reliably maintain the proper temperature for preserving the organ. Can protect you! % without causing foaming of the SIi fluid, it is possible to reduce the manufacturing cost of the device.

[Brief explanation of the drawing]

Figures 1 to 3 (a) and (bl) show one embodiment of the present invention; Figure 1 is a block diagram of the organ perfusion device of the present invention; Figure 2 (a), (1:, ) and FIGS. 3(a) and 3(t)) show a net-like holding member,
FIG. 2(a) is a front view of the receiving part of the net-like holding member, FIG. 2 (CI)) is a side sectional view of the receiving part, and FIG. 3(a) is a front view of the lid of the mesh-like holding member. @ Figure 3 (b) is a side sectional view of the lid, and Figure 4 is a configuration diagram of a conventional organ single-flow device. 7...organ, 15...perfusion pump, 1
6... Heat exchanger, 31... Container body,
33...Lid body, 33a...Air vent, 41...Perfusion circuit, 42...Inlet C outlet connection part)% 43... ...Artificial lung.

Claims (1)

[Claims] The suction port of the perfusion circuit in which the perfusion pump, oxygenator, and heat exchanger are interposed is connected to the lower part of the container body that fills the perfusion fluid, and the outlet of the perfusion circuit is connected to the lower part of the container body that fills the perfusion fluid. A net-like holding member connected to the upper side of the container body for holding the organ is installed approximately horizontally below the outlet connection of the perfusion circuit in the upper part of the container body, and an air vent is provided in the upper part of the container body. An organ perfusion device characterized in that the provided lid body is liquid-tight and detachably attached.