JPS6126867A - Biochemical analysis instrument - Google Patents

Abstract

PURPOSE:To execute easily and exactly pipetting at a good timing by attaching a female block body which rubs with the outside peripheral surface of a male block body provided to a pipete to a partial injecting hole. CONSTITUTION:A disk 5 is rotated and stopped at every installation angle of engaging grooves 10 and measuring elements 11 are inserted successively through an insertion port 20. The sample to be analyzed is then taken into the pipete 23 from a test tube, etc. and is injected partially through the partial injecting port 22. Even if the chip 24 at the top end of the pipete 23 is roughly inserted into the inside hole of the female block body 26, the chip 24 at the top end of the pipete 23 is exactly positioned through the through-hole 12 on top of the groove 10 of the disk 5 toward the center of the measuring plane of the element 11 by the rubbing operation of a male block body 25 and the body 26. The manual pipetting is thus easily and exactly executed at the good timing within the stop time of the disk 5 without being affected by the shake, etc. of the hand.

Description

[Detailed Description of the Invention] [Industrial Application Field] The present invention relates to a biochemical analyzer, and more particularly to a device for chemically analyzing and measuring a liquid sample using a measuring element impregnated with a reaction reagent. .

[Conventional technology]

In general, in liquid samples such as blood and serum, it is often necessary to know whether or not a specific component is contained in the liquid sample, or the content thereof, and for this purpose, chemical analysis using reaction reagents is performed.

There are two methods for chemical analysis of liquid samples: the dry method and the wet method. Of these, the dry method uses a liquid sample measurement element consisting of a thin plate impregnated with a specific reagent sandwiched between mounts. A liquid sample to be analyzed is supplied dropwise to the element, placed in a thermostatic chamber for reaction, and the liquid sample and reagent are allowed to react, and the progress or results of the reaction are monitored.

For example, this method is a method of measuring and detecting a change in color density due to a reaction using an optical density meter or other means, and is very convenient in that a liquid sample can actually be treated as a solid.

However, it is difficult to drop a large number of samples onto a measuring element one by one and measure the change in color density caused by the nine reactions using an optical density meter. Using a disc that can be locked in position,
Biochemical analyzers have been developed in which the disk is installed so that it can be rotated at a constant angle, and the measuring elements are sequentially moved to measurement positions to perform photometry. When dispensing is performed manually, it is difficult to accurately and timely dispense a drop onto the center of the measuring surface of the measuring element due to tremors in the hand or the like.

It was taking time.

[Purpose of the invention]

This invention is intended to solve the above-mentioned problems, and aims to provide a biochemical analyzer that allows pipette dispensing to be performed easily, accurately, and in a timely manner.

[Structure of the invention]

This invention relates to a biochemical analyzer equipped with a disk for mooring and transporting measurement elements, in which an outer circumferential surface is attached to a tip of a pipette inserted through a pipette dispensing hole for a test sample provided on a top plate of a main body that covers the top surface of the disk. A male block body having a downward convergence gradient is attached, and a female block body having a common rubbing surface that rubs against the outer peripheral surface of the male block body is attached to the dispensing hole, so that the pipette can be guided during pipette dispensing. It is configured as follows.

〔Example〕

Next, the present invention will be described based on an embodiment shown in the accompanying drawings.

■ is the main body of the biochemical analyzer (hereinafter referred to as the "main body"),
A constant temperature plate 2 having a circular planar shape is installed on the bottom plate 1b of the main body 1 via leg members (not shown). The constant temperature plate 2 is hollow so as to accommodate a heat retaining medium a such as water, and an electric heater 3 is attached to the lower surface.

Reference numeral 4 denotes a rotating shaft whose upper and lower ends are supported by the upper plate 1a and the bottom plate 1b of the main body 1 through a hole 2a penetrating the center of the thermostatic plate 2. On the upper part of the rotating shaft 4 is a rotary shaft 2 which can be slid in the axial direction. A disk 5 is fitted so as to be fixed in the rotational direction. The disk 5 is kept at a constant temperature by the pressing force of a spring 7 fitted to the rotating shaft 5 and the pressing force of a spring member 8 containing a spring 8'' that presses the ball 8' so that it comes into contact with the upper surface of the peripheral edge of the disk 5. It is designed to be able to rotate closely or close to the top surface of the plate 2.The spring members 8 only need to be provided at several locations equidistributed around the circumference of the disk. A fan 9 is provided to stir the medium.

Reference numeral 10 denotes engagement grooves for the measuring element 11 provided at equally distributed locations on the lower surface of the circumferential edge of the disk 5. The engaging grooves 1° are less than the thickness of the measuring element 11, and the lower surface of the engaged measuring element 10 is disk 5
As it rotates, it comes into sliding contact with the upper surface of the constant temperature plate 2, and is preheated during transportation. A through hole 12 for dispensing a test sample onto the measuring element 11 with a pipette is provided on the upper surface of the disk 5 corresponding to the engaging groove 1o.

Note that 13 is an elongated hole for discharging the measuring element 11 from the engagement groove 10, and 14 is a cross hole for making visible an identification symbol such as a person's name written on the surface of the measuring element 11.

Reference numeral 15 denotes an exposure hole for exposing a code display 11a for specifying the inspection item of the measuring element 11, and a detector 16 installed on the upper plate 1a of the main body 1 corresponds to the exposure hole 15. 1
7 is a code display for specifying the address of the engagement groove located, for example, with respect to the engagement groove 10 of -, and is provided radially on the upper surface of the center portion of the disk 5. Corresponding to the code display 17 is an address detector 18 installed on the upper plate 1a of the main body 1. The inspection items and addresses of the measurement elements read by the detector 16 and the address detector 18 are stored in a storage device (not shown).

19 is a drive source for driving the rotating shaft 4 of the disk 5;
The drive source 19 is connected to the rotating shaft 5 in a heald manner. The rotating shaft 4 is provided with a stopper means (not shown) that allows the locking groove 1o of the measuring element 11 provided on the periphery of the disk 5 to accurately stop at a photometry section and a dispensing section to be described later.

Reference numeral 20 denotes an insertion opening for the measuring element 11 to be inserted into the engagement groove 10 provided on the periphery of the disk 5. The insertion opening 20 is inserted into the side wall of the main body 1 at an appropriate position so as to fit into the engagement groove 10 when the disk 5 is stopped. It is provided. The insertion slot 20 is equipped with a device (not shown) that stacks and accommodates measuring elements for each inspection item and selectively supplies the necessary measuring elements. It is more preferable to allow automatic insertion by means of 21 (as shown).

Reference numeral 22 denotes a pipette dispensing port for dispensing the test sample into the measuring element 11 which is moored in the engagement groove lO of the disk 5 and transported on the thermostatic plate 2. The dispensing hole 22 is located at the stopping position of the measuring element 11. The upper plate 1a of the main body 1 corresponding to the upper plate 1a is provided in a penetrating shape. A pipette 23 is inserted through the dispensing hole 22, and a male block body 25 whose outer peripheral surface has a downward convergence gradient is attached to the distal tip 24 of the pipette 23, as shown in FIG. The male block body 25 can be satisfactorily constructed of a lightweight material such as plastic foam, and its size and cross-sectional shape may be determined arbitrarily. Reference numeral 26 denotes a female block body having a common rubbing surface 26' that rubs against the outer peripheral surface of the male block body 25, and the female block body 26 is attached to the dispensing hole 22. Although the female block body 26 does not necessarily have to be made of a lightweight material, it is preferably made of a material that has enough strength to properly support the fitted male block body 25 and has a slippery property that allows for easy insertion and removal.

Reference numeral 27 denotes a photometry section that optically measures the progress or result of the reaction between the sample to be tested after dispensing and the element of the measurement element 11, and changes in color density and change due to the reaction.

As shown in FIG. 7, the photometric section 27 converts a light beam generated from a light source 28 such as a halogen lamp into a photometric beam of a desired wavelength through a lens 29 and a filter 30, and bends the photometric beam through a mirror 31. The light is guided through an optical fiber 32 to a photometry head 33 installed close to the measurement surface (back surface of the element) of the measurement element 11 and irradiated thereon. This irradiation light is reflected from the measurement surface and transmitted to the light receiving element 34' through the optical fiber 34, and is calculated by a calculation device (not shown) such as a microcomputer, and the measured value is displayed on a display window provided at a suitable location on the main body 1. (not shown). This measured value may be printed on a roll of recording paper (not shown) if necessary.

In addition, 35 is a transparent glass tilted at 45° installed in the optical path of the photometric light beam in order to eliminate as much as possible errors in measurement values due to changes in the light intensity of the photometric light beam over time. A portion of the reflected light is referred to a correction circuit (not shown) via the light receiving element 36 so that the measured value of the photometric light beam reflected from the measuring element can be corrected to a correct value.

37 is a discharge mechanism for discharging the measuring element 11 after being measured, and 38 is a container for storing the discharged measuring element outside the main body.

Next, the effect will be explained.

First, the disk 5 is rotated for each installation angle of the engagement groove 10,
After stopping, the measuring elements 11 are sequentially inserted through the insertion openings 22. The code display on the surface of the inserted measuring element is read by the detector for the analysis item, and the code display on the disc surface is detected by the address detector at which address of the engagement groove of the disk 5 the measuring element for that item is inserted. read and memorized. Thereafter, the operator takes the test sample to be analyzed from a test tube or the like into the pipette 23 and dispenses it from the two-dispense port. In this case, even if the end tip 24 of the Pibeno 1-23 is casually inserted into the inner hole of the female block body 26, the end tip will not move as shown in FIG. The disc 5 is accurately positioned from the through hole in the upper surface of the engagement groove 10 toward the center P of the measurement surface of the measurement element.

Next, the operator operates an operation panel (not shown) to specify an analysis item, or starts the apparatus without specifying, and the disk rotates by the required angle and a stopper mechanism (not shown) moves the specified measuring element. Stop at the top of the photometering head of the photometer. Here, a photometric light beam from a light source is irradiated onto the measurement surface, and the reflected light is processed by a calculation device such as a microcomputer via a light receiving element, and the measured value is displayed on a display window of the main body. At the same time, it is recorded on a roll of recording paper as required.

Then, the measured measurement element is discharged through the discharge mechanism into a storage container provided outside the main body.

〔Effect of the invention〕

As described above, according to the present invention, in a biochemical analyzer equipped with a disk for mooring and transporting measurement elements, a pipette inserted through a pipette dispensing hole for a test sample provided on the upper plate of the main body that covers the upper surface of the disk is provided. A male block body having an outer peripheral surface having a downward convergence gradient is attached to the distal tip, and a female block body having a common rubbing surface that rubs against the outer circumferential surface of the male block body is attached to the dispensing hole. However, when manually dispensing the sample with a pipette during the disk stop time, the distal tip does not deviate significantly from the center of the measurement surface of the measuring element due to hand tremors, etc., making pipette dispensing possible. It has the excellent effect of being simple, accurate, and timely.

[Brief explanation of the drawing]

The figures show one embodiment of the present invention, in which Fig. 1 is a partially cutaway perspective view of the entire device, Fig. 2 is a partially cutaway plan view of the same, and Fig. 3 is the relationship between the measuring element and its locking groove. FIG. 4 is a cross-sectional view of the insertion port of the measuring element, FIG. 5 is an enlarged perspective view of the main part, FIG. 6 is a cross-sectional view during dispensing, and FIG. 7 is a schematic diagram of the photometry section. FIG. 1 - Main body 2 - Constant temperature plate 4 - Rotating shaft 5 - Disc 22 - Pipette dispensing hole 23 - Pipette 24 - Tip 25 - Male block body 26 - Female block body Patent applicant Konishiroku Photo Industry Co., Ltd. Figure 3 Figure 5

Claims (1)

[Claims] In a biochemical analyzer equipped with a disk for mooring and transporting measurement elements, a pipette with the outer peripheral surface facing downward is attached to the tip of the pipette inserted through the pipette dispensing hole for the test sample provided on the upper plate of the main body that covers the upper surface of the disk. A biochemical analysis device characterized in that a male block body having a focusing gradient is attached, and a female block body having a common rubbing surface that rubs against the outer peripheral surface of the male block body is attached to the dispensing hole.