JPS6224745B2 - - Google Patents
Info
- Publication number
- JPS6224745B2 JPS6224745B2 JP11607076A JP11607076A JPS6224745B2 JP S6224745 B2 JPS6224745 B2 JP S6224745B2 JP 11607076 A JP11607076 A JP 11607076A JP 11607076 A JP11607076 A JP 11607076A JP S6224745 B2 JPS6224745 B2 JP S6224745B2
- Authority
- JP
- Japan
- Prior art keywords
- hepatitis
- antibody
- antibodies
- enzyme
- antigen
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 239000000427 antigen Substances 0.000 claims description 98
- 102000036639 antigens Human genes 0.000 claims description 97
- 108091007433 antigens Proteins 0.000 claims description 97
- 208000006454 hepatitis Diseases 0.000 claims description 96
- 231100000283 hepatitis Toxicity 0.000 claims description 96
- 102000004190 Enzymes Human genes 0.000 claims description 52
- 108090000790 Enzymes Proteins 0.000 claims description 52
- 238000000034 method Methods 0.000 claims description 50
- 239000007787 solid Substances 0.000 claims description 12
- 239000000126 substance Substances 0.000 claims description 12
- 239000007788 liquid Substances 0.000 claims description 9
- 239000011541 reaction mixture Substances 0.000 claims description 3
- 239000007790 solid phase Substances 0.000 claims description 3
- 230000002255 enzymatic effect Effects 0.000 claims description 2
- 238000012360 testing method Methods 0.000 description 91
- 229940088598 enzyme Drugs 0.000 description 59
- 239000000523 sample Substances 0.000 description 59
- 239000000243 solution Substances 0.000 description 49
- 210000002966 serum Anatomy 0.000 description 39
- 239000003153 chemical reaction reagent Substances 0.000 description 27
- 239000000758 substrate Substances 0.000 description 27
- 210000004369 blood Anatomy 0.000 description 25
- 239000008280 blood Substances 0.000 description 25
- 238000003018 immunoassay Methods 0.000 description 21
- 239000000872 buffer Substances 0.000 description 18
- 238000011534 incubation Methods 0.000 description 18
- 241000283073 Equus caballus Species 0.000 description 17
- 239000013641 positive control Substances 0.000 description 17
- 239000013642 negative control Substances 0.000 description 16
- 102000006395 Globulins Human genes 0.000 description 15
- 108010044091 Globulins Proteins 0.000 description 15
- 239000010410 layer Substances 0.000 description 15
- 102000004169 proteins and genes Human genes 0.000 description 15
- 108090000623 proteins and genes Proteins 0.000 description 15
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 15
- 239000006228 supernatant Substances 0.000 description 14
- FVAUCKIRQBBSSJ-UHFFFAOYSA-M sodium iodide Chemical compound [Na+].[I-] FVAUCKIRQBBSSJ-UHFFFAOYSA-M 0.000 description 13
- 238000002835 absorbance Methods 0.000 description 12
- 238000004519 manufacturing process Methods 0.000 description 12
- 238000003127 radioimmunoassay Methods 0.000 description 12
- 238000006243 chemical reaction Methods 0.000 description 10
- 238000001514 detection method Methods 0.000 description 10
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 9
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 9
- 208000002672 hepatitis B Diseases 0.000 description 9
- 239000002953 phosphate buffered saline Substances 0.000 description 9
- 210000002381 plasma Anatomy 0.000 description 9
- 238000002360 preparation method Methods 0.000 description 9
- PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 description 8
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 8
- 238000010521 absorption reaction Methods 0.000 description 8
- 239000012153 distilled water Substances 0.000 description 8
- 239000012528 membrane Substances 0.000 description 8
- XZKIHKMTEMTJQX-UHFFFAOYSA-N 4-Nitrophenyl Phosphate Chemical group OP(O)(=O)OC1=CC=C([N+]([O-])=O)C=C1 XZKIHKMTEMTJQX-UHFFFAOYSA-N 0.000 description 7
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 7
- 208000005252 hepatitis A Diseases 0.000 description 7
- 239000008188 pellet Substances 0.000 description 7
- 102000002260 Alkaline Phosphatase Human genes 0.000 description 6
- 108020004774 Alkaline Phosphatase Proteins 0.000 description 6
- 241001465754 Metazoa Species 0.000 description 6
- 239000003610 charcoal Substances 0.000 description 6
- 230000003053 immunization Effects 0.000 description 6
- 239000011777 magnesium Substances 0.000 description 6
- 239000000463 material Substances 0.000 description 6
- 239000011159 matrix material Substances 0.000 description 6
- 239000000203 mixture Substances 0.000 description 6
- 238000005406 washing Methods 0.000 description 6
- 238000002649 immunization Methods 0.000 description 5
- 238000002372 labelling Methods 0.000 description 5
- 230000035945 sensitivity Effects 0.000 description 5
- 239000011780 sodium chloride Substances 0.000 description 5
- 238000012546 transfer Methods 0.000 description 5
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 4
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 description 4
- 208000037319 Hepatitis infectious Diseases 0.000 description 4
- 239000004793 Polystyrene Substances 0.000 description 4
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 4
- 229940098773 bovine serum albumin Drugs 0.000 description 4
- 238000005119 centrifugation Methods 0.000 description 4
- 239000003085 diluting agent Substances 0.000 description 4
- 238000001914 filtration Methods 0.000 description 4
- 229920000642 polymer Polymers 0.000 description 4
- 229920002223 polystyrene Polymers 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 235000009518 sodium iodide Nutrition 0.000 description 4
- 238000010998 test method Methods 0.000 description 4
- 241000283086 Equidae Species 0.000 description 3
- 239000007864 aqueous solution Substances 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 210000001124 body fluid Anatomy 0.000 description 3
- 239000010839 body fluid Substances 0.000 description 3
- 239000007795 chemical reaction product Substances 0.000 description 3
- 230000000295 complement effect Effects 0.000 description 3
- 230000003247 decreasing effect Effects 0.000 description 3
- 238000000502 dialysis Methods 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 229940079593 drug Drugs 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 239000002244 precipitate Substances 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 238000000746 purification Methods 0.000 description 3
- 238000012216 screening Methods 0.000 description 3
- 239000000377 silicon dioxide Substances 0.000 description 3
- 238000003756 stirring Methods 0.000 description 3
- XZKIHKMTEMTJQX-UHFFFAOYSA-L 4-nitrophenyl phosphate(2-) Chemical compound [O-][N+](=O)C1=CC=C(OP([O-])([O-])=O)C=C1 XZKIHKMTEMTJQX-UHFFFAOYSA-L 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- 206010019799 Hepatitis viral Diseases 0.000 description 2
- 206010029719 Nonspecific reaction Diseases 0.000 description 2
- 102000004160 Phosphoric Monoester Hydrolases Human genes 0.000 description 2
- 108090000608 Phosphoric Monoester Hydrolases Proteins 0.000 description 2
- 206010036790 Productive cough Diseases 0.000 description 2
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 2
- 230000002776 aggregation Effects 0.000 description 2
- 238000004220 aggregation Methods 0.000 description 2
- 238000013019 agitation Methods 0.000 description 2
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 2
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 2
- 235000011130 ammonium sulphate Nutrition 0.000 description 2
- 210000000601 blood cell Anatomy 0.000 description 2
- 239000007853 buffer solution Substances 0.000 description 2
- 229910052799 carbon Inorganic materials 0.000 description 2
- 235000011089 carbon dioxide Nutrition 0.000 description 2
- 230000003197 catalytic effect Effects 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 238000012790 confirmation Methods 0.000 description 2
- 238000007796 conventional method Methods 0.000 description 2
- 238000001962 electrophoresis Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000012467 final product Substances 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 229920000578 graft copolymer Polymers 0.000 description 2
- 150000003278 haem Chemical class 0.000 description 2
- 230000001900 immune effect Effects 0.000 description 2
- 238000000760 immunoelectrophoresis Methods 0.000 description 2
- 230000001965 increasing effect Effects 0.000 description 2
- 230000001939 inductive effect Effects 0.000 description 2
- 208000015181 infectious disease Diseases 0.000 description 2
- 239000002198 insoluble material Substances 0.000 description 2
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 230000003287 optical effect Effects 0.000 description 2
- -1 polytetrafluoroethylene backbone Polymers 0.000 description 2
- 239000003755 preservative agent Substances 0.000 description 2
- 230000002335 preservative effect Effects 0.000 description 2
- 230000009257 reactivity Effects 0.000 description 2
- 230000002441 reversible effect Effects 0.000 description 2
- 229920006395 saturated elastomer Polymers 0.000 description 2
- 239000011734 sodium Substances 0.000 description 2
- 229910000029 sodium carbonate Inorganic materials 0.000 description 2
- 210000003802 sputum Anatomy 0.000 description 2
- 208000024794 sputum Diseases 0.000 description 2
- 201000001862 viral hepatitis Diseases 0.000 description 2
- YHWIVSWMIVTOGT-UHFFFAOYSA-N 2-isothiocyanatoethenylbenzene Chemical compound S=C=NC=CC1=CC=CC=C1 YHWIVSWMIVTOGT-UHFFFAOYSA-N 0.000 description 1
- 102100031126 6-phosphogluconolactonase Human genes 0.000 description 1
- 108010029731 6-phosphogluconolactonase Proteins 0.000 description 1
- 229910002019 Aerosil® 380 Inorganic materials 0.000 description 1
- 101710187573 Alcohol dehydrogenase 2 Proteins 0.000 description 1
- 101710133776 Alcohol dehydrogenase class-3 Proteins 0.000 description 1
- 108010024957 Ascorbate Oxidase Proteins 0.000 description 1
- 102100026189 Beta-galactosidase Human genes 0.000 description 1
- 241000283690 Bos taurus Species 0.000 description 1
- 108090000209 Carbonic anhydrases Proteins 0.000 description 1
- 102000003846 Carbonic anhydrases Human genes 0.000 description 1
- 102000000496 Carboxypeptidases A Human genes 0.000 description 1
- 108010080937 Carboxypeptidases A Proteins 0.000 description 1
- 102100035882 Catalase Human genes 0.000 description 1
- 108010053835 Catalase Proteins 0.000 description 1
- 102000030523 Catechol oxidase Human genes 0.000 description 1
- 108010031396 Catechol oxidase Proteins 0.000 description 1
- 102000003914 Cholinesterases Human genes 0.000 description 1
- 108090000322 Cholinesterases Proteins 0.000 description 1
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 1
- 102000004674 D-amino-acid oxidase Human genes 0.000 description 1
- 108010003989 D-amino-acid oxidase Proteins 0.000 description 1
- 101710088194 Dehydrogenase Proteins 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 238000002965 ELISA Methods 0.000 description 1
- 102000001390 Fructose-Bisphosphate Aldolase Human genes 0.000 description 1
- 108010068561 Fructose-Bisphosphate Aldolase Proteins 0.000 description 1
- 108010015133 Galactose oxidase Proteins 0.000 description 1
- 101000892220 Geobacillus thermodenitrificans (strain NG80-2) Long-chain-alcohol dehydrogenase 1 Proteins 0.000 description 1
- 108010018962 Glucosephosphate Dehydrogenase Proteins 0.000 description 1
- 102000053187 Glucuronidase Human genes 0.000 description 1
- 108010060309 Glucuronidase Proteins 0.000 description 1
- 108010000445 Glycerate dehydrogenase Proteins 0.000 description 1
- 108010038519 Glyoxylate reductase Proteins 0.000 description 1
- 102000001554 Hemoglobins Human genes 0.000 description 1
- 108010054147 Hemoglobins Proteins 0.000 description 1
- 208000005176 Hepatitis C Diseases 0.000 description 1
- 102000030789 Histidine Ammonia-Lyase Human genes 0.000 description 1
- 108700006308 Histidine ammonia-lyases Proteins 0.000 description 1
- 108091006905 Human Serum Albumin Proteins 0.000 description 1
- 102000008100 Human Serum Albumin Human genes 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 102000009617 Inorganic Pyrophosphatase Human genes 0.000 description 1
- 108010009595 Inorganic Pyrophosphatase Proteins 0.000 description 1
- 102000004877 Insulin Human genes 0.000 description 1
- 108090001061 Insulin Proteins 0.000 description 1
- 102100024319 Intestinal-type alkaline phosphatase Human genes 0.000 description 1
- 101710184243 Intestinal-type alkaline phosphatase Proteins 0.000 description 1
- 108010008292 L-Amino Acid Oxidase Proteins 0.000 description 1
- 102000007070 L-amino-acid oxidase Human genes 0.000 description 1
- 102000003855 L-lactate dehydrogenase Human genes 0.000 description 1
- 108700023483 L-lactate dehydrogenases Proteins 0.000 description 1
- 102000004895 Lipoproteins Human genes 0.000 description 1
- 108090001030 Lipoproteins Proteins 0.000 description 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
- 108010026217 Malate Dehydrogenase Proteins 0.000 description 1
- 102000013460 Malate Dehydrogenase Human genes 0.000 description 1
- BAVYZALUXZFZLV-UHFFFAOYSA-N Methylamine Chemical compound NC BAVYZALUXZFZLV-UHFFFAOYSA-N 0.000 description 1
- 102000016943 Muramidase Human genes 0.000 description 1
- 108010014251 Muramidase Proteins 0.000 description 1
- 108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 101710171235 Peroxidase 16 Proteins 0.000 description 1
- 229920001213 Polysorbate 20 Polymers 0.000 description 1
- 108010071390 Serum Albumin Proteins 0.000 description 1
- 102000007562 Serum Albumin Human genes 0.000 description 1
- PMZURENOXWZQFD-UHFFFAOYSA-L Sodium Sulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=O PMZURENOXWZQFD-UHFFFAOYSA-L 0.000 description 1
- 101000874347 Streptococcus agalactiae IgA FC receptor Proteins 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 1
- 239000007983 Tris buffer Substances 0.000 description 1
- 102000014384 Type C Phospholipases Human genes 0.000 description 1
- 108010079194 Type C Phospholipases Proteins 0.000 description 1
- 108010046334 Urease Proteins 0.000 description 1
- 239000002250 absorbent Substances 0.000 description 1
- 230000002745 absorbent Effects 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 102000004139 alpha-Amylases Human genes 0.000 description 1
- 108090000637 alpha-Amylases Proteins 0.000 description 1
- 229940024171 alpha-amylase Drugs 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 108010005774 beta-Galactosidase Proteins 0.000 description 1
- 210000000013 bile duct Anatomy 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- 239000007975 buffered saline Substances 0.000 description 1
- 244000309466 calf Species 0.000 description 1
- 239000003054 catalyst Substances 0.000 description 1
- 210000001175 cerebrospinal fluid Anatomy 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 229940048961 cholinesterase Drugs 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 230000009918 complex formation Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 230000001268 conjugating effect Effects 0.000 description 1
- 238000012864 cross contamination Methods 0.000 description 1
- 230000000593 degrading effect Effects 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000012470 diluted sample Substances 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000001493 electron microscopy Methods 0.000 description 1
- 239000000839 emulsion Substances 0.000 description 1
- 238000006911 enzymatic reaction Methods 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 238000011049 filling Methods 0.000 description 1
- 238000011010 flushing procedure Methods 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 231100000206 health hazard Toxicity 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 125000004029 hydroxymethyl group Chemical group [H]OC([H])([H])* 0.000 description 1
- 230000003100 immobilizing effect Effects 0.000 description 1
- 230000008105 immune reaction Effects 0.000 description 1
- 230000000951 immunodiffusion Effects 0.000 description 1
- 230000016784 immunoglobulin production Effects 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 229940125396 insulin Drugs 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 230000000155 isotopic effect Effects 0.000 description 1
- 238000011005 laboratory method Methods 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 229960000274 lysozyme Drugs 0.000 description 1
- 235000010335 lysozyme Nutrition 0.000 description 1
- 239000004325 lysozyme Substances 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- 235000010355 mannitol Nutrition 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 229930014626 natural product Natural products 0.000 description 1
- 238000006386 neutralization reaction Methods 0.000 description 1
- 229940127240 opiate Drugs 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 125000000636 p-nitrophenyl group Chemical group [H]C1=C([H])C(=C([H])C([H])=C1*)[N+]([O-])=O 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 150000003014 phosphoric acid esters Chemical class 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- 238000002616 plasmapheresis Methods 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 1
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 1
- 229920001343 polytetrafluoroethylene Polymers 0.000 description 1
- 239000004810 polytetrafluoroethylene Substances 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 230000002829 reductive effect Effects 0.000 description 1
- 230000000395 remineralizing effect Effects 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 238000012552 review Methods 0.000 description 1
- 210000003296 saliva Anatomy 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 235000017557 sodium bicarbonate Nutrition 0.000 description 1
- 229910000030 sodium bicarbonate Inorganic materials 0.000 description 1
- 239000001488 sodium phosphate Substances 0.000 description 1
- 229910000162 sodium phosphate Inorganic materials 0.000 description 1
- 229910052938 sodium sulfate Inorganic materials 0.000 description 1
- 235000011152 sodium sulphate Nutrition 0.000 description 1
- AKHNMLFCWUSKQB-UHFFFAOYSA-L sodium thiosulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=S AKHNMLFCWUSKQB-UHFFFAOYSA-L 0.000 description 1
- 235000019345 sodium thiosulphate Nutrition 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 238000000527 sonication Methods 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 239000008174 sterile solution Substances 0.000 description 1
- 150000003431 steroids Chemical class 0.000 description 1
- 239000002344 surface layer Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
- RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 description 1
- 238000005199 ultracentrifugation Methods 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
- 239000013598 vector Substances 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- 210000004885 white matter Anatomy 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/576—Immunoassay; Biospecific binding assay; Materials therefor for hepatitis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/544—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being organic
- G01N33/545—Synthetic resin
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Food Science & Technology (AREA)
- Biochemistry (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Communicable Diseases (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Peptides Or Proteins (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Description
æ¬çºæã¯æŠããèèçã«é¢é£ããã¢ã³ãã²ã³ã®
ååšãæ€åºããæ¹æ³ã«ä¿ããç¹å®ããã«ãæ¬çºæ
ã¯ãèèçã®ååšãæ€åºãã¹ãé µçŽ ã§æšèããã
æäœãçšããŠã¢ã³ãã²ã³ãšåå¿ãããããšãå å«
ããèèçã«é¢é£ããã¢ã³ãã²ã³ã®å ç«æž¬å®ã«ä¿
ãã ãèèã®ççããæå³ããèèçã¯ãè管ãæ
æãåã¯éå¡ããããšã«ãããããŒã«ã¹æ§èèç
ã«ã¯ïŒçš®é¡åšã€ãŠãäžæ¹ãä»ã®ãã®ããæœäŒæã
é·ããšèãããããåŸåãæ£è ãèèçã«ããã
æ¢ç¥ã®éçµå£çæŽé²ãæãããšãããã®èèçã¯
ãè¡æž æ§èçããšãããããããæ£è ãæ¢ç¥ã®é
çµå£çæŽé²ãæããçµå£çã«èèçã«ããã€ãŠã
ãã°ãããã¯ãäŒææ§èçããšåŒã°ãããããã
ãªãããæç®çã§æ å ±æäŸãããŠããããã«ãäž¡
è ã¯æœäŒæãéãªãåãããã®äžãäŒææ§èçã
ã§ãã€ãŠãéçµå£çã«ãããããšããããæè¬
ãè¡æž æ§ãèçã§ãã€ãŠãçµå£çã«ãããããšã
ãããããããŠãèèçã«ã¯å°ããšãïŒçš®ã®éã€
ãåªä»ã«ãã€ãŠåŒãèµ·ãããäºã€ã®ã¿ã€ãããã
ããã«æãããããããããèå¥ããã®ã«çšèª
ãè¡æž æ§èçãããã³ãäŒææ§èçããçšããã¹
ãã§ãªããåŸã€ãŠããäŒææ§èçãã«æãè¿äŒŒã
ãã¿ã€ãã®èçã«ã¯ãèïŒèïŒçããçšããã¹
ãã§ããããŸããè¡æž æ§èçãã«æãè¿äŒŒããŠã
ããã®ã«ã¯ïŒèïŒèïŒçããçšããã¹ãããšã
瀺åãããŠããã æ¬æçްæžã«èšèŒã®ãäŸãã¯èèçã«é¢é£ãã
ã¢ã³ãã²ã³ã®æ€åºã«æåãããèããŠãè¡æž æ§è
çã«æãè¿äŒŒããèèçã«ããã€ãŠããæ£è ã¯ã
ãããã©ããªçç¶ã§ããããã°ãã°è¡æ¶²äžã«äžèš
ã¢ã³ãã²ã³ãæããŠããããã®æç¹ã§æ³šæãã¹ã
ããšã¯ãèèçåã¯ä»®å®ãããèèçã«é¢é£
ããã¢ã³ãã²ã³ã®ååšã枬å®ããããã®ä¿¡é Œãã
ãæ¹æ³ããªããšããããšã§ãããããããŠãæ¬æ
现æžäžã®äŸã¯èèçã«é¢é£ããã¢ã³ãã²ã³ã®å
åšãæ€åºããããšã«æåãããããããªãããå¥
ã¿ã€ãã®èèçã«é¢é£ããã¢ã³ãã²ã³ã§ãã€ãŠã
ãããäžåºŠåå®ããããªããã®ã¢ã³ãã²ã³ã®ååš
ãæ€åºããã®ã«æ¬çºæã®æ¹æ³ã䜿çšããåŸãªãç
ç±ã¯ãªãã ãè¡æž æ§èçããªããèèçã«ããããšãç¡
èŠããããšã®ã§ããªãèé ·ãªèšåºåé¡ãåµåºã
ããèããŠãåé¡ãèé ·ãªããæ ã«ãèèçãæ€
åºããããã®è©Šé𿹿³ãçš®ã éçºããããããã
è©Šéšæ¹æ³ã«ããã¯ãã»ã¢ãŠãã¿ã©ããŒïŒMicroâ
OuchterlonyïŒãå ç«æ¡æ£ãè£äœçµåãå ç«é»æ°
æ»²éæ³ïŒImmunoelectroâosmophoresisïŒããã
åéããã³ãã åéæå¶ãé»åé¡åŸ®é¡ããªãã³ã«
åºçžæŸå°å ç«æž¬å®ãå å«ãããããããã®è©Šéšæ¹
æ³ã¯åã ãããªãã€ãã·ãŠã»ã¡ãžã«ã«ã»ããªãã³
ïŒBritish Medical BulletinïŒã1972ãVol.28ãNo.
ïŒïŒãŠã€ã«ã¹æ§èçïŒãP138ã141ã«è©³è¿°ãããŠ
ããã®ã§å¿ èŠã«å¿ããããåç §ããããã å ç«é»æ°æ»²éæ³åã¯é黿°æ³³åæ³ïŒCEPïŒ
ã¯ãèèçã«é¢é£ããã¢ã³ãã²ã³ïŒä»¥äžææèç
é¢é£ã¢ã³ãã²ã³åã¯åã«èçã¢ã³ãã²ã³ãšåŒç§°ã
ãïŒãšãã®æäœïŒä»¥äžåã«èçæäœãšåŒã¶ïŒãæ€
åºããè¿ éäžã€ç°¡åãªæ¹æ³ãäŸäžãããããããª
ããããã®æè¡ã¯ãäŸãã°è£äœçµåã«æ¯ã¹è¥å¹²æ
床ã«ãããŠå£ããèããŠããã®äž»ãªå©ç¹ã¯ãïŒæ
éå ã§è©Šéšãå®äºããããšããããšã§ããããã
ãããªãããæåºŠã¬ãã«ãäœãããã«ãCEPã¯
é£åå»è¬ç®¡çå±ïŒthe Food and Drug
AdministrationïŒã«ãã€ãŠãã¯ãèªå¯ãããŠããª
ãã æŸå°å ç«æž¬å®æ³ïŒRIAïŒã¯ã詊éšãè¡ãã®ã«å¿
èŠãªè£ çœ®ãæ¯èŒçè€éãç¹æ®ãªé«äŸ¡è£ 眮ã§ããã°
ããã§ãªããæŸå°æ§åäœå çŽ ïŒã©ãžãªã¢ã€ãœããŒ
ãïŒãåæ±ãéå³éãªäºé²çãèŠæ±ãããã®ã§ã
ãã®æè¡ãæ¥åžžèšºæç®çã«é©çšããããšã¯è¥å¹²å¶
éããããã®ãšä¿¡ããããã®äžãã©ãžãªã¢ã€ãœã
ãŒãæšèã¯éå€§ãªæœåšçå¥åº·å±éºãåããç£èŠã
ãã³ååãšãã«ã®ãŒå§å¡äŒïŒAtomic Energy
CommissionïŒã®ïŒäœ¿çšããã³è£œé ã«å¯ŸããïŒå
èš±ãå¿ èŠãšããäžã€å»æ£ç©åŠçåé¡ãåãããã«
ããããããããã®æè¡ã¯ä»æ¥ãå ç«æž¬å®ããã®
ã«ããªãããå®çããŠããã ç¡è«ãå ç«åŠçæ¹æ³ã¯ãå šãŠã®æäœããã³ã¢ã³
ãã²ã³ã®äž»èŠç¹æ§ããªãã¡ç¹å®ã®è£äœã¢ã³ãã²ã³
åã¯æäœãšã®åå¿æ§ã«äŸæ ãããããããŠããã
æäœããã®ã¢ã³ãã²ã³ã嫿ããè¡æž ã«æ·»å ãã
ããªããæäœãšã¢ã³ãã²ã³ã¯é¯åœ¢æãèããŠæº¶æ¶²
ããæåºããããšãã§ãããã»ãšãã©ã®åèšè©Šéš
æ¹æ³ã«ãããŠããã®ç°¡åãªäºå®ãå©çšããããšã«
ãããäººè¡æž äžã®ã¢ã³ãã²ã³ã®ååšãæ€åºãã
ãã ãããŸã§ãæšèæäœãçš®ã ã®ã¢ã³ãã²ã³ãåå®
ããããã«çšããããŠããããããç¹å®ã®ã¢ã³ã
ã²ã³ã«å¯Ÿãç¹ç°æ§ã§ããããšãããã€ãŠããæäœ
ãã該æäœç£æ§ã®ããåºæ¿ãããå¯äž»åç©ã®è¡æž
åã¯è¡ãããã®ã°ãããªã³éšåããåé¢ãããª
ãããã®æäœã¯æ¢ç¥æ¹æ³ã«ãã€ãŠæšèããããšã
ã§ããããã®æäœããæšèå€äŸãã°äžè¿°ã®åŠãã©
ãžãªã¢ã€ãœããŒãåã¯è¢å è¬åã®ãããªç©ççã«
æ€åºã§ããç©è³ªãšçµåãããããšã«ãããæäœã®
ååšãæ€åºããããšãã§ãããããããŠãæšèæ
äœã蚺æã«çšãããšãã察å¿ããã¢ã³ãã²ã³ãæ
ã調補詊éšè©Šæäžã«ååšããã°ã該æšèæäœã¯ã
ã®ã¢ã³ãã²ã³ãšçµåããèããŠè©Šéšè©Šæäžã®è©²æš
èæäœãæ€åºããããšã«ããã¢ã³ãã²ã³ã®ååšã
確èªããããã ãã®èšºæçšæšèæäœã¯ããããæ€åºãææãã
ãã¢ã³ãã²ã³ãšã ãåå¿ã該ã¢ã³ãã²ã³ãšã¯å šã
ç°ãªãããã»ã©éèŠã§ãªãä»ã®è¿çžã¢ã³ãã²ã³ãš
çžäºåå¿ããªãããã«ååç¹ç°æ§ã§ããã¹ãã§ã
ããããããŠããããªãå ç«æž¬å®ã«ãããŠããæ
äœã®çµŠæºããã³æäœã®ç£çæ¹æ³ãããããå šãèš
ççã§ãããšããããšã¯æããã§ããã åè¿°ã®åŠããèèçã«é¢é£ããã¢ã³ãã²ã³ãæ€
åºããæ¹æ³ã®äžã€ã«åºçžæŸå°å ç«æž¬å®æ³ãå å«ã
ããããã®æé ã¯ç±³åœç¹èš±ç¬¬3867517å·ã«é瀺ã
ããŠããã該ç¹èš±ã«é瀺ãããåŠããæŸå°å ç«æž¬
å®ãè¡ãéã該枬å®ã«çšããããæåœ¢ããªã¹ãã¬
ã³ã®ããŠãŒããŠãšã«ãªããã€ã³ãµãŒããå ãæäœ
ã§è¢«èŠããããããã¯ã被èŠãã¹ãè©²éšæãæäœ
溶液ãšäžç·ã«æž©çœ®ããããšã«ãã€ãŠéæãããã
ãã®åŸãæªç¥è©Šæãããã®æäœè¢«èŠããããŠãŒã
ãŠãšã«ãªããã€ã³ãµãŒããšå ±ã«æž©çœ®ããŠæäœãšè©²
詊æäžã«ååšããã¢ã³ãã²ã³ãšãåå¿ããããã
ãŠãŒããŠãšã«ã¯æ¬¡ãã§æŽæµãããæŽã«æŸå°æ§åäœ
äœïŒ©â125ã§æšèãããæäœãšå ±ã«æž©çœ®ãããã
ååºŠã®æŽæµã«ãã€ãŠãæªçµåã®æšèæäœãå šãŠå
ãé€ããããããããŠã詊éšè©Šæäžã«ã¢ã³ãã²ã³
ãååšããå Žåãããªã¹ãã¬ã³ã®ããŠãŒããŠãšã«
ïŒãªããã€ã³ãµãŒãïŒãæäœãã¢ã³ãã²ã³ããã³ïŒ©
â125æšèæäœãããªããµã³ãã€ããã圢æãã
ããæ¬¡ãã§ããã®ïŒ©â125æšèæäœããåºãæŸå°
ç·ãèšæ°åšã«ãã€ãŠæž¬å®ããå¯Ÿç §ãšæ¯èŒãããã ãŸããæŸå°å ç«æž¬å®ãè¡ãéã眮æããªã¹ãã¬
ã³äŸãã°ã€ãœããªã·ã¢ãã¹ãã¬ã³ãã°ã©ãããã
ãããªããããã«ãªã«ãšãã¬ã³ã®åæ¿ãæçšã§ã
ãããããšãé瀺ãããŠããããã®ããªã¹ãã¬ã³
ã¯ãããçœè³ªãšå ±æçµåããŠçç©è©Šéšã«æçšãªè©Š
è¬ãããããããããçœè³ªåå¿æ§åºã®ç¹æ®èšèšè¡š
é¢ããã€äžæº¶æ§ç©è³ªã§ãããæè¿ãæäœãã©ãžãª
ã¢ã€ãœããŒãåã¯è¢å è¬åã§æšèããããšã«åã€
ãŠä»£ãéèŠãªæ¹æ³ãéçºããããããã¯ãæäœã
é µçŽ ã§æšèããæ¹æ³ã§ããããã®æé ã«ã€ããŠ
ã¯ãç±³åœç¹èš±ç¬¬3654090å·ããã³å第3791932å·ã«
èšèŒãããŠããããŸããã¯ãªãã«ã»ã±ãã«ã«ã»ã¢
ã¯ã¿ïŒClinika chemical ActaïŒã48ïŒ1973ïŒã15
ã18ã«ã¯ããµã³ãã€ããæ³åã¯ç«¶åããããã«ã
ãã¢ã«ãã¢ããšããããã€ã³ã®é µçŽ çµååŒå ç«æž¬
å®ãé瀺ãããŠããã é µçŽ æšèã¯ãæŸå°å ç«æž¬å®ã«æ¯èŒããŠããã€ã
ã®éèŠãªå©ç¹ãæããŠãããäŸãã°ãæçµæ··åç©
äžã®é µçŽ æšèååã¯å šãŠãèªã¿åãã«åäžããã
仿¹ãæçµæ··åç©äžã®ã¢ã€ãœããŒãååã¯ãèªã¿
åãã®éããå°å²åãã厩å£ããèããŠè©²å²åã®
ã¿ãæŸå°å ç«æž¬å®ã«åäžããã«ãããªãããŸãã
é µçŽ æšèã§ã¯ãæ€åºå¯èœãªæçµçæç©ã圢æãã¹
ãå€ãã®åºè³ªååãæ»æããããšã«ãããå šãŠã®
æšèãèªã¿åãã«ãäŸãã°100000åïŒminãŸã§å
埩åäžãããããããæåºŠã倧巟ã«é«ãããã
ãïŒã¢ã€ãœããŒãæšèååã¯èªã¿åãã®éïŒåºŠã
ã厩å£ãããã®åŸã¯åäžããªããªããé µçŽ æšè詊
è¬ã¯ä¿å寿åœãé·ããïŒã¢ã€ãœããŒãæšè詊è¬ã¯
çµ¶ãã厩å£ãã€ã€ããããã®ä¿å寿åœåé¡ã¯èé ·
ã§ããããŸããé µçŽ æšè詊è¬ã®äœ¿çšã«é¢é£ããå¥
康å±éºã¯æå°ã®ãã®ã§ããããã¢ã€ãœããŒãæšè
ã®å Žåããã¯é倧ã§ãããæŽã«ãŸããé µçŽ æšèã«
ããå ç«æž¬å®ã¯ãç°¡åã§äžã€æ¯èŒçå®äŸ¡ãªèªã¿å
ãè£ çœ®ãçšããããšãã§ãããïŒã¢ã€ãœããŒãã«
ããå ç«æž¬å®ã®éŠå°Ÿã¯ããããšèããçžéããŠåŽ©
å£ã®æ€åºå¹çã«äŸæ ãèããŠãŸããããããŠé«äŸ¡
ã«ã€ãæ€åºè£ çœ®ã®æ§èœã«äŸæ ããã 人ã®è¡æ¶²äžã«èçã¢ã³ãã²ã³ãèŠåºãããšã¯ã
èèçã®èšåºæŽãååŸããããšãšåçã§ã¯ãªãã
ããããªãããçš®ã ã®ç ç©¶ã«ãã€ãŠãèçã¢ã³ã
ã²ã³ã«é¢ãéœæ§ãšåºãè¡æ¶²ãæ£è ãå容ãããšã
èçææã®çºç ãé«ããšããã€ãããã°ãã°ãè¡
æ¶²ã»ã³ã¿ãŒããå ¥æãããç¹å®ã®è¡æ¶²ãŠãããã
䜿çšãããåŠãããæ¯èŒççæéã§æ±ºå®ããã°ãª
ããªãã®ã§ãèèçã®ã¹ã¯ãªãŒãã³ã°ãã¹ãïŒéž
å¥è©ŠéšïŒãé«äŸ¡ãªè£ 眮ãå¿ èŠãšããããšãªã髿
床ãè¿ éäžã€å®¹æã«è¡ãããšã¯ããããŠéèŠã§ã
ããèèçã«é¢é£ããã¢ã³ãã²ã³ã®æ€åºã«åŸæ¥çš
ããããçš®ã ã®è©Šéšã¯æãçšåºŠæºè¶³ãããŠããã
ãã©ãããããããã¯å šãŠäžã€åã¯äºã€ä»¥äžã®æ¬
ç¹ãæããã æ¯å¹Žèèçã«ãã€ãŠäœå人ãã®äººãæ»ã«äžã€å ¥
é¢ããŠãããé·ãéããã®ç æ°ãæå¶ããéµã¯ã
äžççã«æå¹ã§ãããŸã容æäžã€æ¥åžžçã«è¡ãã
ãè¡æ¶²éžå¥æè¡ã§ãããšèããããŠãããæ¢è¿°ã®
ããã«ãããã€ãã®æè¡ã仿¥å容ãããŠããã
ãã©ãããããã¯æ¯èŒçäœæåºŠã§ãããç¹éäžã€
æéã®ãããæé ãå¿ èŠãšããã»ãšãã©ã®ãã¢ã
ããžãŒã©ãïŒhemotology labïŒã«ãããŠå®¹æã«
ã¯å©çšãåŸãªãè€éãªè£ 眮ã䜿çšããã°ãªããã
æã¯ãéåžžã«äžå®å®ã§æè¿ãã«ã¯ä¿å šãåŸãªã詊
è¬ãçšããã°ãªããªããããæ ããããã®æè¡ã¯
çæ³çãªè©Šéšã®èŠæ±ãæºãããã®ã§ã¯ãªãã€ãã æŠæ¬ããã«ãæ¬çºæã¯ãããµã³ãã€ãããåç
ãçšããèçã¢ã³ãã²ã³ã®çŽæ¥çå ç«æž¬å®ãå å«
ããããã®æž¬å®ãè¡ãã«éããæ€åºãããšããã¢
ã³ãã²ã³ãããããšåå¿ããæäœå±€ã®éã«ã¯ã
ããäžæ¹ã®æäœå±€ã¯é µçŽ ã§æšèãããŠãããä»ã®
å±€ã¯äžæº¶æ§éšæã«å ±æçµåããŠãããé µçŽ ã¯ãå
åŠåºè³ªã«ããããããã該åºè³ªã¯é µçŽ è§Šåªã®ååš
ã§ååŠå€åãèµ·ãããŠåå¿çæç©ã圢æãããè
èçã®æç¡ã¯ããã®åå¿çæç©ãå®éããããšã«
ãã€ãŠæ±ºå®ãããã æ¬çºæã®å¥ã®æ§çžã«åŸãã°ãèèçã®æ€åºãã
ããªããã»ãããäŸäžãããããã®æ€åºã»ããã®
äžå€§è©Šè¬ã¯ãèèçã«é¢é£ããã¢ã³ãã²ã³ãšåå¿
ããæäœãçµåãããäžæº¶æ§éåäœåºäœãé µçŽ ã§
æšèãããèçæäœè©Šè¬ããã³ãé µçŽ ã®è§Šåªç圱
é¿äžååŠçã«å€åããŠæ€åºå¯èœãªæçµçæç©ã圢
æããããšã®ã§ããé µçŽ åºè³ªã§ãããéžã°ããå ·
äœåã§ã¯ãçµåäœã®é µçŽ ãã¢ã«ã«ãªæ§ãã¹ãã¢ã¿
ãŒãŒïŒããé žé µçŽ ïŒã§ãããé µçŽ åºè³ªãïœâãã
ãããšãã«ãã¹ããšãŒãã§ãããäžèšæ€åºã»ãã
ã«ã¯ãŸããèçã¢ã³ãã²ã³ã«å¯Ÿãé°æ§ãåŒ±éœæ§ã
ãã³åŒ·éœæ§ã®è¡æž ãå å«ããå¯Ÿç §è¡æž ã詊éšè©Šæ
âäžæº¶æ§éåäœåºäœéã®éç¹ç°æ§åå¿ã®é »åºŠãæ
å°éã«ãã詊éšè©ŠæçšéЬã°ãããªã³æ·»å å€ã®æº¶
æ¶²ãé µçŽ åºè³ªæº¶æ¶²ã®PHãåå¿ã«æé©ãªç¯å²ã«ä¿æ
ãã¹ãäŒå³ãããç·©è¡æ¶²ããªãã³ã«æž©çœ®ã§çšãã
ããå°éã®è©Šè¬âäžæº¶æ§éåäœåºäœéã®æ¥è§Šãé«
ããããã«èšç®ããã寞æ³ã®è€æ°åã®ãã€ã¢ã«
ïŒå°ã³ãïŒãå«ãŸãããé µçŽ âé µçŽ åºè³ªç³»ãšããŠ
ã¢ã«ã«ãªæ§ãã¹ãã¢ã¿ãŒãŒããã³ïœâãããããš
ãã«ãã¹ããšãŒããçšãããšããç·©è¡æ¶²ã¯
Na2CO30.028Mããã³Mgã0.001Mã®æ°Žæº¶æ¶²ã§ã
ãã æ¬çºæã®å¥ã®æ æ§ã«ãããŠãã¢ã«ã«ãªæ§ãã¹ã
ã¢ã¿ãŒãŒã®åŠãå®èœé µçŽ ãšååŠçã«çµåãããå
ç«åŠäžæŽ»æ§ãªç²Ÿè£œèçæäœãå ç«æž¬å®æ³ã«çšãã
ããã¹ãäŸäžãããã æ¬çºæã®æŽã«å¥ã®æ æ§ã«ãããŠãåæ¿æ§äžæº¶æ§
éšæãªãããããªãã¯ã¹ãå ç«æž¬å®æ³ã«çšããã
ãã¹ãäŸäžãããããã®ãããªãã¯ã¹ã¯ãã®è¡šé¢
å±€ã«ãããçœè³ªãšåå¿ããè€æ°åã®åºããäžæ§ã«
ã°ã©ãããããŠããã粟補ãããèçæäœã¯ãã®
åå¿åºã«å ±æçµåããŠèçæäœã®å€é¢å±€ãããã
ãã å ç«æž¬å®æ³ã«ãããŠãäžèšã®äžæº¶æ§éšæã¯å¹³åº
ã®ãã€ã¢ã«ã«å ¥ããããäžã€æ¶²äœè©Šéšè©Šæã§çšã
ãèŠãããã詊éšãåçŸæ§ã§ããããã«ã¯ã詊éš
詊æäžã®ã¢ã³ãã²ã³ãæäœã§è¢«èŠãããåæ¿ã®è¡š
é¢å šäœã«æŽé²ãããŠããããšãéèŠã§ãããå¹³ã
ãªè¡šé¢ããã€åæ¿ã®äœ¿çšã¯æåºŠæå€±ã«åž°ãããšã
çºèŠããããåŸã€ãŠãæ¬çºæã®åæ¿ã®äž¡é¢ã¯ãå
æ¿é¢âãã€ã¢ã«åºã®æ¥è§Šãå°ãããããã«æãäž
æŽåœ¢ç¶ã«å€åœ¢ãããŠãããéžã°ããäžã€ã®å ·äœå
ã§ã¯ãæäœãçµåãããã«å ç«ã€ãŠåæ¿ããã¬ã¹
ã«éãããšã«ããããã®äž¡é¢ãçŒãç¶²æ§ãªããæ Œ
å圢ã«ãããã ããæ ãæ¬çºæã®äž»èŠç®çã¯ãäœæ¶²äžã®èèç
ã«é¢é£ããã¢ã³ãã²ã³ã®æç¡ãè¿ éãç°¡åãæ£ç¢º
äžã€æ¥åžžçã«æ±ºå®ããããããªèèçã®æ€åºãã
ãããã³ãã®äœ¿ç𿹿³ãæç€ºããããšã§ããã æ¬çºæã®å¥ã®ç®çã¯ãæ¯èŒçæªçãªäººãèèç
ã«é¢é£ããã¢ã³ãã²ã³ã®é«æåºŠåçŸæ§å ç«æž¬å®æ³
ãæ¥åžžåºæºã§å®æœããã®ã«é©åããåäœå詊éšã»
ãããæäŸããããšã§ããã æ¬çºæã®å¥ã®ç®çã¯ãå ç«æž¬å®æ³ã宿œããã«
éãæé äžã®èª€ããæå°éã«ãã¹ãäŒå³ãããåŠ
äžã®è©Šéšã»ããã«ããŠãå¿ èŠãªè©Šè¬ãåå¿å®¹åšç
ãå šãŠã詊éšã®ç²ŸåºŠããã³æåºŠãæé©ã«ãã¹ãäŒ
å³ããã圢ã§å ¥ãã該詊éšã»ãããæäŸããããš
ã§ããã æ¬çºæã®å¥ã®ç®çã¯ã詊éšã®é宿œãããçš®ã
ã®å·¥çšãæšæºåããããã«äŒå³ããã詊éšã»ãã
å ¥ãå®éšå®€è£ 眮ãæäŸããããšã§ããã æ¬çºæã®æŽã«å¥ã®ç®çã¯ã詊éšè©Šæã®æ¯èŒåºæº
ãšããŠãèçé¢é£ã¢ã³ãã²ã³ã«å¯Ÿãé°æ§ãåŒ±éœæ§
ããã³åŒ·éœæ§ã®å¯Ÿç §è©Šæãå«ã詊éšã»ãããæäŸ
ããããšã§ããã æ¬çºæã®å¥ã®ç®çã¯ãå ç«æž¬å®æ³ã§çšãããã
èçæäœâé µçŽ çµåäœã«ããŠãæäœãšé µçŽ ãšãå
åã®ææãããååŠç¹æ§ãä¿æãããåŠã該çµå
äœãæäŸããããšã§ããã æ¬çºæã®ä»ã®ç®çã¯ãå ç«åŠçç¹æ§ãªããè§Šåª
ç¹æ§ãæå€±ããããšãªãé·æéä¿åããããšã®ã§
ããåŠäžã®çµåäœã«ããŠãèèçã«é¢é£ããã¢ã³
ãã²ã³ãšé«åºŠã«åå¿ããããã®ãæäŸããããšã§
ããã æ¬çºæã®æŽã«å¥ã®ç®çã¯ãåå¿ã«è§Šåªäœçšãå
ãŒããŠæçµçæç©ãé«ãåå¿é床ã§ããããããš
ã®ã§ããé µçŽ ãšçµåããèçæäœãæäŸããããš
ã§ãããèããŠãäžèšæçµçæç©ã®æ¿åºŠã¯ã枬å
忀åºåšãçšããŠæ£ç¢ºã«æ€åºããããã æ¬çºæã®å¥ã®ç®çã¯ãäžè¿°ã®åŠãçµåäœã«ã
ãŠãé«åºŠã«ç²Ÿè£œããèããŠãèèçã«é¢é£ããã¢
ã³ãã²ã³ã®é«æåºŠãé«ç²ŸåºŠå ç«æž¬å®ãä¿é²ããã®
ã«å€§ãã«è²¢ç®ãããšããã®è©²çµåäœãæäŸããã
ãšã§ããã æ¬çºæã®å¥ã®ç®çã¯ãæ¢è¿°ã®è©Šéšã§èšããåŠã
æçšãªäžæº¶æ§ãããªãã¯ã¹ã«ããŠããã®è¡šé¢å šäœ
ã«é«æ¿åºŠã®ç²Ÿè£œèçæäœãäžæ§ã«ååžããããã®
ãæäŸããããšã§ããã æ¬çºæã®ä»ã®ç®çã¯ãèçæäœãåºäœã«çµåã
ããããšã«ãã€ãŠãããã䜿çšäžåãããããã
ãªãæ©æ¢°åãªããã¯ååŠçåã«ãã€ãŠé€å»ãããª
ãããã«ããããšã§ããããã®ç¹ã«é¢ããŠçµ¶å¯Ÿã«
å¿ èŠãªããšã¯ãäžèšçµåãè¡ãéæäœãå®èœæ§ã®
ãŸãã§ããããã«ããããšããªãã¡æäœããã®å
ç«åå¿ã«åäžã§ãèããŠè©²çµåã«ãã€ãŠå ç«ååŠ
çã«å€æ§ãããªãããã«ããããšã§ããã æ¬çºæã®ä»ã®ç®çã¯ãå ç«æž¬å®ã®éæäœã§è¢«èŠ
ãããäžæº¶æ§åºäœãåæ±ããããããã«äžã€ãŸ
ããæž©çœ®ã®éäœ¿çšæº¶æ¶²ã«ãã®è¢«èŠè¡šé¢ã®ããå€ã
éšåãå®éæŽé²ãããããã«è©²äžæº¶æ§åºäœã®åœ¢ç¶
ãæ¹åããããšã§ããã åŠäžã®ç®çããã³ä»ã®ç®çãªãã³ã«æ¬çºæã®ç¹
城ã¯ãäžã€ã®éžå®ãããèããŠå¥œãŸããå ·äœåã
以äžèª¬ç€ºããããšã«ãã€ãŠåœæ¥è ã«ã¯æãããšãª
ããã ãã®åã«ãæ·»ä»å³é¢ãç°¡åã«èª¬æãããªãã 第ïŒå³ã¯ãæ¬çºæã®æ¹æ³ã®å·¥çšãèŠçŽããæµã
å³ã§ããã第ïŒå³ã¯ãæ¬çºæã®æ¹æ³ããã³è©Šéšã»
ããã«çšããããéžå®ãããäžæº¶æ§éšæã®éèŠå³
ã§ããã 第ïŒå³ïŒç¬¬ïŒå³ããã³ç¬¬ïŒå³ã¯ãæ¬çºæã®å ç«
æž¬å®æ³ã®äžã€ã®æž©çœ®æ®µéãå³ç€ºãã 第ïŒå³ã¯ãæ¬çºæã«åŸã€ãŠè©Šéšããã詊éšè©Šæ
ã®éœæ§å ç«æž¬å®ã®éçŸåºããããµã³ãã€ãããæ§
é ã®æŠç¥ç衚瀺ã§ããã 奜ãŸããå ·äœåã®èª¬æ å ããæ¬çºæããã®æãåºãå šæ§çžã«ãããŠè©³
è¿°ãããæ¬çºæã«åŸã€ãèèçã®æ€åºæ¹æ³ã¯ã第
ïŒå³ã«äŸç€ºããåŠãåã€ã®ç°ãªã段éã§å®æœãã
ãã 第ïŒã®æ®µéã¯ãäžæº¶æ§éšæïŒïŒïŒç¬¬ïŒå³åç §ïŒ
äžã«åºå®ãããæäœã第ïŒå³ã«å³ç€ºããåŠã詊éš
è¡æž äžã«ååšããã¢ã³ãã²ã³ãšåå¿ãããããšã
å å«ããããã®åå¿ã¯ã¢ã³ãã²ã³ãåºå®ããèã
ãŠè©²ã¢ã³ãã²ã³ã¯ã第ïŒå³ã«ç€ºãåŠã第ïŒã®å·¥çš
ã§é µçŽ æšèãããæäœãšåå¿ããŠãè©²é µçŽ æšèã
ããæäœããåºå®ããããã«ãªãã第ïŒå³ã«ç€ºã
åŠããé µçŽ ã¯é©åœãªåºè³ªã«ããããã該åºè³ªã¯é µ
çŽ ã®äœçšãåããŠè²ã®å€åããããïŒç¬¬ïŒã®æ®µ
éïŒããã®è²å€åã¯ãèèçã«é¢é£ããã¢ã³ãã²
ã³ã®ååšãç€ºãææšãšãªããæåŸã®ç¬¬ïŒæ®µéã¯ã
è²ã®åºŠåããèªã¿äžã€ãããå¯Ÿç §åºæºã§åŸããã
å€ãšæ¯èŒããããšãå å«ããã äžã«æŠèšãã詊éšã«å¿ èŠãšããããã®å šãŠãå«
ãæ¬çºæã®èçæ€åºã»ããã¯100åã®è©Šéšã«åã
ããäŒå³ããããæãããªåŠãã以äžã«é瀺ãã
詊è¬ã®éçãæ¯äŸçã«å æžããããšã«ããããã
ããã倧ããªåã¯å°ããªèŠæš¡ã®ã»ããã補é ãã
ããšãã§ããã詊è¬ããã³å¯Ÿç §ãå«ããããã¯äŸ
ãã°ç®±ã®äžã«é åãããŠãäœ¿çšæãŸã§ïŒãïŒâã§
ä¿åããããæŽã«ã100åã®è©Šéšãè¡ãã®ã«åå
ãªåæ°ã®ãã€ã¢ã«ãåãä»ããããã ãŸãã第ïŒå³ã«ç€ºãåŠãæ Œå圢ã«äŒŒã衚é¢ãæ
ãããã®å€åŽã«ã¯èçã¢ã³ãã²ã³ãšåå¿ããæäœ
å±€ãçµåãåçµä¹Ÿç¥ãããéåäœåæ¿ã100ã105
ååãä»ããããããããäžæº¶æ§åºäœåæ¿ã®æ£ç¢º
ãªæ§è³ªãšãã®è£œé æ³ã«ã€ããŠã¯åŸã§é瀺ããã 詊éšã»ããã®ç¬¬ïŒã®è©Šè¬ã¯ãèçé¢é£æäœâã¢
ã«ã«ãªæ§ãã¹ãã¢ã¿ãŒãŒçµåäœã®è©Šæãããªãã
ãã®è©Šè¬ã®æ£ç¢ºãªæ§è³ªããã®è£œé æ³ããã³æçšãª
代æ¿è©Šè¬ã«ã€ããŠã¯åŸã§é瀺ããããã®è©Šè¬ã¯ã
ãã®ãŸã䜿çšã«äŸãããæº¶æ¶²ãšããŠæ¯çµŠãããã 詊éšã»ããã®ç¬¬ïŒã®è©Šè¬ã¯ã400mgã®ïœâãã
ãããšãã«ãã¹ããšãŒãé µçŽ åºè³ªã§ããããã®å
åç©ã¯ãç²æ«åœ¢ã§å®å®ã§ãããããããç·©è¡æ¶²ã«
溶解ãããŠïŒmgïŒmlã®æº¶æ¶²ã«ãããšãã¯æ¯èŒçäž
å®å®ãšãªããåŸã€ãŠããããæº¶æ¶²ã¯äœ¿çšçŽåã«èª¿
補ããªããã°ãªããªãã 詊éšã®éã«çšããããä»ã®ç©è³ªã«ãæãéã®éЬ
ã°ãããªã³ã詊æåžéå€ãªãã³ã«çš®ã ã®ç·©è¡æ¶²ã
ãã³æŽæµæ¶²ãå å«ãããããããã®è©Šè¬ããã³ç©
è³ªã®æ£ç¢ºãªæ§è³ªããã³è£œé æ³ã«ã€ããŠã¯åŸã§è¿°ã¹
ãã  æäœã®ç£ç æ¬çºæã宿œããããã«ãèèçã«é¢é£ããã¢
ã³ãã²ã³ãšåå¿ããæäœãååŸããããšãå¿ èŠã§
ãããæ³šæãã¹ãã¯ããããæäœãååšãããšã
ãããšèããŠãæ¬çºæãç¹å®ã®æäœã®äœ¿çšã«éå®
ããã€ããã¯ãªããšããããšã§ããã åå¿æ§æäœã¯ãæ¢ç¥ã®ã¢ã³ãã²ã³ãªããæå詊
æãæ³šå ¥ãããå¯äž»åç©ããæ¡è¡ãããã®ã粟補
ããããšã«ãã€ãŠçšæããããšãã§ãããèèç
ã«é¢é£ããã¢ã³ãã²ã³ãšåå¿ããæäœã¯æŠããè±
åœç¹èš±ç¬¬1387625å·ã«é瀺ãããæ¹æ³ã«ãã€ãŠç£
çããããã èçæäœã®ç£çã¯å ããèçã«éé£ããã¢ã³ã
ã²ã³ã«ã€ããŠéœæ§ã§ããããšãããã€ãŠããè¡æ¶²
ã®ååŸã«äŸæ ãããåŸã€ãŠãçš®ã ã®çµŠæºããåŸã
ããè¡æ¶²ãŠãããã¯å ãããããæ¬çºæã®ç²Ÿè£œã
ããå ç«ç¹ç°æ§æäœãç£çããã®ã«é©ããŠããã
ã©ãããæ±ºå®ãã¹ãè©äŸ¡ãããã°ãªããªãã èçã¢ã³ãã²ã³ã«é¢ãéœæ§ãšæãããè¡æ¶²è¢ã»
ã°ã¡ã³ããïŒãïŒâã§åçŽäœçœ®ã«ä¿ã¡è¡çãäžå
åã«èœã¡çãããã«ããã該è¡çããè¡ãããã
åé¢ããããèããŠããã®åžéåã®è¡ããããšã
ããŠççé£å¡©æ°Žã§ïŒïŒ16ã«åžéãã詊æã®ãåºæº
æäœã«å¯Ÿããå䟡ãåšç¥ã®é黿°æ³³åïŒCEPïŒ
æ³ã«ãã€ãŠè©ŠéšãããåžéããŠãªãè¡ããããš
ïŒïŒ16ã«åžéãã詊æãããããéœæ§ã§ãããšã
ã¯ããã®è¡æ¶²ãŠãããã¯ãåŸè¿°ã®åŠãæ¬çºæã®ç²Ÿ
補æäœã®èª¿è£œçšã«å容ããããšèªããããã è¡æ¶²ãŠãããããåé¢ãããã¢ã³ãã²ã³éšå
ã¯ãå¯äž»åç©äœå ã§ã®æäœç£çãåºæ¿ããã®ã«çš
ããããæã¯è©²åç©ã«ãã€ãŠç£çãããæäœã粟
補ããã®ã«çšãããããã¢ã³ãã²ã³ããããã«çš
ããããã«ããããã®åã«ãããååé¢ããã»ã¹
ã«ä»ããªããã°ãªããªãã è¡ãããã¯ç¡èã®æžå§å®¹åšã«ç§»ãããããã§ã
CaCl2ã®5M溶液ããè¡ããã200mlã«ã€ã
Cacl20.75mlã®åºæºã§å ããããšã«ããååºãã
ããæ¬¡ãã§ããã®æº¶æ¶²ã氎济äž37âã§ïŒæé枩眮
ãåã¯ãåå¡ã圢æãããŸã§æž©çœ®ãããå ãåå¡
ã圢æããåŸãè¡ããããâ20âã§åçµããäžã€
ïŒãïŒâã§èè§£ãããŠåå¡ãåçž®ãããããè¡æž
ãåå¡ããåé¢ããããããå¿ èŠã«å¿ãéãã
ãšããã®ãã®ã¯ãã®ãŸããå ç«åçšèçã¢ã³ãã²
ã³ãã¬ããã®è£œé ã«äŸãããæã¯å ç«åžåå¡ã§ã®
䜿çšã«äŸãããã (1) å ç«åçšã¢ã³ãã²ã³ãã¬ããã®è£œé äžèšããã»ã¹ã«ä»ããèçéœæ§è¡æž ãïŒâã§30
åé10000rpmã®é å¿åé¢ã«ãããããã®é å¿å
é¢ã®äžæŸã¿æ¶²ãè¶ é å¿ç®¡ã«é åããŠãäŸãã°ãã
ã¯ãã³ïŒBeckmanïŒL2â65Bæ©ã§ïŒâãïŒã20æ
é40000ã50000rpmã®è¶ é å¿ã«ä»ãããå管å ã®
äžæŸã¿æ¶²ãåãåºãã廿£ãããã¢ã³ãã²ã³ãå«
ããã¬ããã¯ãèŠå®ïŒ0.15MïŒé£å¡©é¡ã§åããã
ããã æ¬¡ãã§ãåé å¿ç®¡ã«å°å®¹éã®ççé£å¡©æ°Žãå
ãã管å ã®å 容ç©ãé³é¿æ¯åïŒsonicationïŒã«ä»
ããŠãããã厩å£ããããæ¬¡ãã§ãåãœãã±ãŒã¿
ããŠãŒãå ã®æžæ¿ç©ãäžç·ã«ãïŒããŒã«ããïŒã
ããå床ãççé£å¡©æ°Žã®å ¥ã€ãæž æµãªããŠãŒãã«
çé åããããã®æº¶æ¶²ãåã³ãäžèšã®åŠãããã
ã¯ãã³L2â65Bé å¿æ©ã§ïŒâãïŒã20æé40000
ã50000rpmã§é å¿ããã äžæ®µã«èšèŒã®æé ã¯ïŒåãªãããã以äžå埩ã
ãããšãã§ããã åé å¿ç®¡ããäžæŸã¿æ¶²ãåãé€ããåŸããã¬ã
ãç©è³ªãæå°å®¹éã®ççé£å¡©æ°Žäžã«ããŒã«ããã
詊æãåºæºèçæäœã«å¯ŸããŠåæããããããã¬
ãããCEPã«ããïŒïŒ25åã¯ãã以äžã®å䟡ã
瀺ããªãããããå ç«åã«çšããããšãã§ããã
ããŒã«ããã¢ã³ãã²ã³ãã¬ãããïŒmlãã€ã®åå
éã«åããŠå°æ¥ã®äœ¿çšã«åãâ20âã§åçµããã (2) èçæäœã®ç£çããã³å粟補 äžè¿°ã®åŠã調æŽããã¢ã³ãã²ã³ãã¬ããã®è©Šæ
ããå ç«åã®æãç容ã®ããã€ã³ãïŒFreundïŒ
å®å šè£å©æ¶²ã«æ·»å ããŠãåœæ¥è ã«åšç¥ã®æé ã«åŸ
ããšãã«ãžãšã³ã調補ãããæ¬¡ãã§ã該ã¢ã³ãã²
ã³ãå¯äž»åç©äŸãã°éЬã«ãããèªäœæ¢ç¥ã®æè¡ã«
åŸã泚å°ããŠèçæäœãçºçãããå ç«æ§ãä»äž
ãã銬ããæ¡è¡ããæã¯ãã®éŠ¬ãæ £çšæè¡ã«ãã
è¡ãããæ¬åºæ³ïŒplasmapheresisïŒã«ä»ããã
奿³ãšããŠåã¯è¿œå çã«ãä»ã®å ç«ä»äžã«ãŒãã§
è£å©æ¶²äžåšã®èª¿å€ãçšããããšãã§ããã æ¡åããè¡æ¶²ã¯ãåŸã®å ç«åžåâ粟補工çšãäº
æããŠèçæäœãåé¢ãã¹ãåŠçããã°ãªããª
ããæŠæ¬ããã«ããã®å粟補ã¯ïŒæ®µéã§éè¡ãã
ããå ããå¯äž»åç©ããã®è¡ããããåç³ç°åã
ããæ¬¡ãã§ãäžæº¶æ§ã¢ã³ãã²ã³âæäœã®é¯åœ¢æã
æ¹èµ·ããããšã«ããèçé¢é£æäœä»¥å€ã®æäœãæ²
éãããã®ã«ååéã®æ£åžžäººè¡ãããïŒNHPïŒ
ãšäžã§åŸãè¡æž ãšãæ··åãããåžåãããæè¡æž
ããCEPã䜿ã€ãŠèçæäœã«é¢ãåæãããæ
åŸã«ãèçã«é¢é£ããã¢ã³ãã²ã³ãšåå¿ããæäœ
ãç¡«é žã¢ã³ã¢ããŠã ã§æ²æ®¿ãããããã®ç©è³ªã¯äœ¿
çšæãŸã§åçµããããšãã§ããã (3) å ç«åžåå€ãã€ãŒã³ãŒã«å¡ã®è£œé æ¬çºæã®è©Šè¬ãçæããã®ã«çšãããã粟補æ
äœã®è£œé ã¯ãäžè¿°ã®åŠãç£çãããèçæäœãå
ç«ç¹ç°æ§æœåºããã»ã¹ã«ä»ãããšã«ããéæãã
ãããã®æé ã®ç·èª¬ãããã¢ã³ãã²ã³ãšæäœã®å
ç«åŸç°æ§åé¢ãïŒImmunospecific Separation of
Antigens and AutibodiesïŒããšé¡ãããã©ãã€
ãã·ãŠïŒBradishïŒçã®1975å¹ŽïŒæ19æ¥ä»ãè±åœ
ç¹èš±ç¬¬1387625å·ã«èšãããŠããã®ã§å¿ èŠã«å¿ã
ãããåç §ããããã äžè¬ã«ããã®ç²Ÿè£œããã»ã¹ãæ¬çºæã§å©çšãã
ãšããããã¯ãæäœãèçã¢ã³ãã²ã³ãšåå¿ããŠ
é¯åœ¢æãèããŠãæ¡è¡ããæœåºãããæäœè©Šæäž
ã«äžå¯é¿çã«ååšããä»ã®å€æ¥æäœããã³ããçœ
質ãé€å€ãããšããæäœã®èœåãå©çšããã åæŽæµããåžåå€ã«ãŒãã³ãã¬ã©ã¹ç®¡ãªããã
ã©ã¹ããã¯ç®¡ã«æ £çšæè¡ãçšããŠå ãŠãããããš
ã«ãããå¡ãçšæãããèçã¢ã³ãã²ã³ã®ç°ãªã
æ··æç©ãåŸãããã«ãå¥åã®è¡æž 詊æå°ããšãïŒ
åããã¢ã³ãã²ã³ãããŒã«ãããæ¬¡ãã§ãããŒã«
ããã¢ã³ãã²ã³ããæŽå€åžåãåºã«ããŠïŒãïŒ
mgïŒmlïŒæº¶åªïŒã®ããçœè³ªæ¿åºŠã«èª¿æŽããã ã¢ã³ãã²ã³ãã«ãŒãã³ã«ä»çãããã¹ãããã®
åžæº¶æ¶²ã300ã1000mlïŒhrç¯å²ã®æµéã§åžåå¡ã«
å°å ¥ããããã®éãåžåå¡å ã®ãã€ãŒã³ãŒã«ïŒïœ
åœã75mgã®ããçœè³ªãæ·»å ããããã«ãã¹ãã§ã
ããåžåå¡ããã®æµåºç©ã500mlã®ååéã§é
ãããã®åã ãããçœè³ªå«éã«ã€ããŠæ€æ»ããã
æµåºç©ãåºçºç©è³ªã®ããçœè³ªå«éã«ã»ãçãã該
å«éã瀺ããšããåžåå¡ã¯ã¢ã³ãã²ã³ã§é£œåãã
ããšèªããããããã€ãŒã³ãŒã«å±€ã¯ãæµåºç©ã
280nmã«ãããŠç®ç«ã€çšã®æ€åºå¯èœãªåžåã瀺ã
ãªããªããŸã§å¡ã«ç¡«é žå¡©ç·©è¡å€å ¥ãé£å¡©æ°Ž
ïŒPBSïŒãæµãããšã«ãã€ãŠããã€ãŒã³ãŒã«å±€ã
æŽæµããã ãããçµåããããçœè³ªã溶é¢ãã¹ãããã€ãŒ
ã³ãŒã«å±€ãæ°ãã調補ããããªç¡«é žãããªãŠã
200mgïŒå ¥ã5MããåãããªãŠã æ¶²ã§ãã©ãã·
ãŠïŒæŽãæµãïŒããããã®åŸãåžåå¡ã«ååéã®
PBSãæµãå ¥ããŠã該å¡ããäžèšã®ããåãããª
ãŠã æ¶²ãæµå»ãããèããŠãïŒmgïŒmlã®ãããªãŠ
ã ã¢ãžãïŒé²è å€ïŒã嫿ããPBCã§åžåå±€ãæ
çµçã«æŽæµããåŸãå¡ãïŒãïŒâã«ä¿åããããš
ã«ãããåŸã®äœ¿çšã«åããããšãã§ããã (4) é µçŽ çµåäœã«çšããããæäœããã³äžæº¶æ§åº
äœã®è£œé äžè¿°ã®åŠã粟補ããæäœã¯ç¡«é žã¢ã³ã¢ããŠã ã
ãªãããããPBSã«ãã€ãŠãæäœïŒéšå¯Ÿç·©è¡å€ïŒ
éšã®åºæºã§åžéãããå¡ãæŽåããç»åãéãã
ããããã«äœçœ®ã¥ããããã®å¡ã«ãäžèšã®æäœæº¶
æ¶²ãçŽ200mlïŒhrã®æµéã§é£ç¶çã«æ·»å ãããæµ
åºç©ãéãããããããçœè³ªããã³èçæäœå«é
ã«ã€ããŠè©Šéšããããšã«ãããå¡ããã€æäœã§é£œ
åãããããæ±ºå®ããã飜åãéããããåŸãå¡
ã®å±€ãPBSã§æŽæµããŠãããåžåãããããçœè³ª
ãé€å»ããã ãã®æç¹ã§ããã€ãŒã³ãŒã«å±€ã«åºå®ããã¢ã³ã
ã²ã³ã¯ããã®ãã®ãšåå¿ããæäœãšçµåããŠã
ãããŸããå¡ãéã€ãŠããä»ã®å€æ¥ããçœè³ªãã
ã³æäœã¯ããã®å±€ã«åºå®ããã¢ã³ãã²ã³ã«å¯ŸããŠ
éç¹ç°æ§ã§ãããèããŠäžèšæäœããåé¢ããŠã
ãã ãã®æäœâã¢ã³ãã²ã³çµåãç Žæãäžã€ç²Ÿè£œæ
äœã溶é¢ãã¹ãã䜿çšçŽåã«èª¿è£œããNaIã®5M溶
æ¶²ãå¡ã«å°å ¥ããããã®NaI溶液ã®äœ¿çšå®¹éã¯ã
å±€ã«çµåããæäœãå šãŠåãåºãã®ã«ååã§ãã
ã¹ãã§ããã塿µéãå°ããšã20mlïŒhrã«èšå®ã
ããšããæº¶åºæ¶²ãé©åœãªå®¹éã®ç»åã§éããã
ããéãŸã€ãåèšéã¯å°ããšããããåãããªãŠ
ã ïŒNAIïŒæº¶æ¶²ã®æ·»å 容éã«çããããã¹ãã§ã
ãã 粟補æäœã®åç»åã¯ãéãããããšäºåã®é
ã«ä»ããããããªãã¡ãäžåç®ã¯0.45ÎŒã®èã«é
ããäºåç®ã¯0.22ÎŒã®èã«éããæ¬¡ãã§ãïŒãïŒ
âã®èžçæ°ŽãçšããŠãåŸãããæ¶²ãïŒïŒïŒã«åž
éãããäŸãã°200mlã®æ¶²ã40mlã®èžçæ°Žã«æ·»
å ãããæ¬¡ãã§ãåžéãããããæäœç»åããäŸ
ãã°ãXMâ50ã®èãåããã¢ãã³ã³
ïŒAMICONïŒæ¿çž®æ©ã«å ¥ããŠæ¿çž®ããã æçµç²Ÿè£œå·¥çšãšããŠããã®æ¿çž®ããã粟補æäœ
ãéæãããéæã®åŸãæäœãåãåºããŠé å¿å
é¢ã«ããããäžæŸã¿æ¶²ãå°ããšã20æé0.01Mã®
ç¡«é žãããªãŠã 溶液ã«å¯ŸããŠéæãããããPBS
ã«å¯Ÿããåãã®éæãšæ¯èŒããããã®åŸã®éæã
çµäºããåŸãæäœã®ããçœè³ªæ¿åºŠã枬å®ããã æ¬¡ãã§ããã®æäœã«ã€ããŠãCEPæè¡ã«åŸã
åºæºã¢ã³ãã²ã³ã«å¯ŸããæŽ»æ§åºŠã枬å®ããŠæäœå«
éãæ±ºå®ãããŸãããåãå ¥ãããããšããã€ã
ãããããåçµä¹Ÿç¥ããŠäœ¿çšæãŸã§ä¿åããã  æäœâé µçŽ çµåäœã®è£œé åçã®è žå ã¢ã«ã«ãªæ§ãã¹ãã¢ã¿ãŒãŒãåæ§æ
ããæäœã®æº¶æ¶²ã«ãïŒïŒïŒã®é µçŽ ïŒæäœæ¯ã§æ··å
ããããšã«ãããæçµæ¿åºŠãBPSïŒPH7.4ïŒæº¶æ¶²
ïŒmlã«ã€ãå šããçœè³ª10mg以äžã«ãªãããã«ã
ãããã®æº¶æ¶²ãéæããŠNH4 +ã€ãªã³ãå®å šã«é€
å»ããã éæåŠçãããæäœâé µçŽ æ··åç©ã次ãã§é å¿
åé¢ã«ããäžæº¶æ§ç©è³ªãå šãŠé€ããPBSâMgã
æº¶æ¶²ã®æ·»å ã«ãããäžæŸã¿æ¶²ã®ããçœè³ªå«éã10
mgïŒmlã«èª¿æŽããããã®æº¶æ¶²ã«ïŒïŒ ã®ã°ã«ã¿ã«ã¢
ã«ãããæº¶æ¶²ããæäœâé µçŽ æº¶æ¶²10mlã«ã€ãã°ã«
ã¿ã«ã¢ã«ãããæº¶æ¶²ïŒmlã®åºæºã§æ·»å ããã3.5
ã20åéç·©åŸã«æ¹æãèããŠãã®éãæäœãšé µçŽ
ãã°ã«ã¿ã«ã¢ã«ãããã«ãã€ãŠååŠçµåããåŸã
ãã®çµåäœã®æº¶æ¶²ã0.001Mã®Mgãå ¥ãPBCã«å¯Ÿ
ããŠéæããŠã°ã«ã¿ã«ã¢ã«ããããé€å»ããã æ¬¡ãã§ãéæåŠçããç©è³ªãé å¿åé¢ã«ããã
åŸãããäžæŸã¿æ¶²ã«ããªã¹ïŒããããã·ã¡ãã«ïŒ
ã¢ããã¡ã¿ã³ïŒPHïŒïŒç·©è¡å€0.05Mãæ£åžžäººã¢ã«
ããã³ïŒçµæ¶è³ªïŒïŒïŒ ãNaN30.02ïŒ ããã³
MgCl20.001Mã®æ°Žæº¶æ¶²ãæ·»å ããŠãããåžéã
ãã ã¢ã«ã«ãªæ§ãã¹ãã¢ã¿ãŒãŒã«å ããŠãä»ã®é µçŽ
ã亊æ¬çºæã®æ¹æ³ã«çšããããšãã§ãããäºå®ã
æäœã«å ±æçµåãããé µçŽ ãšããŠã»ãšãã©ç¡éã®
ãã®ãæããããããããçš®ã ã®é µçŽ ã®ãã¡ä»¥äž
ã«åæãããã®ãç¹ã«æå©ã§ããã ïŒ ã¢ã«ã³ãŒã«è±æ°ŽçŽ é µçŽ ïŒ ã°ãªã»ããŒã«è±æ°ŽçŽ é µçŽ ïŒ ã°ãªãªãã·ã¬ãŒãéå é µçŽ ïŒ ïŒ¬âä¹³é žè±æ°ŽçŽ é µçŽ ïŒ ãããé žè±æ°ŽçŽ é µçŽ ïŒ ã°ã«ã³ãŒã¹ïŒâããé žè±æ°ŽçŽ é µçŽ ïŒ ãã³ãããïŒâããé žè±æ°ŽçŽ é µçŽ ïŒ ã°ã«ã³ãŒã«é žåé µçŽ ïŒ ã¬ã©ã¯ããŒã¹é žåé µçŽ 10 âã¢ããé žé žåé µçŽ 11 âã¢ããé žé žåé µçŽ 12 ããªããšããŒã«é žåé µçŽ 13 ã¢ã¹ã³ã«ãã³é žé žåé µçŽ 14 ã«ã¿ã©ãŒãŒ 15 ãã«ãªãã·ããŒãŒ 16 ã³ãªã³ãšã¹ãã©ãŒãŒ 17 ãã¹ããªãªããŒãŒïŒ£ 18 αâã¢ãã©ãŒãŒ 19 ãªãŸããŒã 20 βâã¬ã©ã¯ãã·ããŒãŒ 21 ã¢ããã°ã«ã·ããŒãŒ 22 βâã°ã«ã¯ããããŒãŒ 23 ã«ã«ããã·ãããããŒãŒïŒ¡ 24 ãŠã¬ã¢ãŒãŒ 25 ç¡æ©ãããã¹ãã¢ã¿ãŒãŒ 26 ã¢ã«ãã©ãŒãŒ 27 çé žè±æ°Žé µçŽ 28 ãã¹ãããŒãŒ æäœãæšèããã®ã«çšããããé µçŽ ã¯ãããã€
ãã®èæ ®ãã¹ãäºæã«çæããŠéžå®ãããããã
ãçæãã¹ãäºæã«ã¯ãé µçŽ ã®å®å®æ§é µçŽ ã®æž¬å®
å®¹ææ§ãæäœã®å ±æçµåã®æ¡ä»¶ã«å¯Ÿããé µçŽ ã®è
ä¹ æ§ãªãã³ã«é µçŽ ã®å ¥ææ§ããã³äŸ¡æ Œãå å«ãã
ãã  æäœã§è¢«èŠãããäžæº¶æ§éšæã®è£œé 詊è¬è£œé ã®æ¬¡ã®å·¥çšã¯ã粟補æäœã®äžéšåãäž
溶æ§éšæã«å ±æçµåãããããšã§ããããã®çµå
ãè¡ãããã«ãçšããããäžæº¶æ§éšæã¯ãåå¿åº
åã¯çç©è©Šéšã«çšããããç¹ç°æ§æäœãšåå¿ãã
ãéšäœãäžããããŠããªããã°ãªããªãã ãã°ã©ããã»ã³ããªããŒãºïŒGraft
CopolymersïŒããšé¡ããG.W.ãã¬ã®ã¢ãŒ
ïŒTregearïŒçã®ç±³åœç¹èš±ç¬¬3700609å·ã«ã¯ãæã
éåäœäž»éã«å¥ã®éåäœåã¯å ±éåäœã®åŽéãã°
ã©ãããããŠãªãäžæº¶æ§é£ç¶éåäœç©è³ªãé瀺ã
ããŠããããã®ã°ã©ãããããéåäœãé©å®éžå®
ããããšã«ãã€ãŠãçç©ç©è³ªãäžæº¶æ§ãããªãã¯
ã¹ã«ååŠçµåãããããšãã§ãããäžèšç¹èš±ã«é
瀺ãããçæç©ã¯ãã€ã³ããªã¢ã«ã»ã±ãã«ã«ã»ã€
ã³ãã¹ããªãŒãºã»ãªãã»ãªãŠã¹ãã¬ã€ãªã¢ã»ã¢ã³
ãã»ããŠãŒãžãŒã©ã³ãïŒICIANZïŒããåååã
ãã¿ããŒã«ïŒPROTAPOLïŒDIïŒïŒã®åæ¿åœ¢ã§åž
販ãããŠããã ããã¿ããŒã«DIïŒïŒã¯ãã€ãœããªã·ã¢ãããª
ã¹ãã¬ã³åºã衚é¢å šäœã«ã°ã©ãããããããªãã
ã©ãã«ãªããšãã¬ã³äž»éãããªããæŸå°å ç«æž¬å®
æ³ã«äœ¿çšãã¹ãäŒå³ãããŠãããçŸåšå ¥æããã
ããã®çš®ã®åæ¿ã¯ãåããçŽ0.01ã€ã³ããçŽåŸã
çŽ0.5ã€ã³ãã§ããã 第ïŒå³ã«ç€ºãåŠãæ¬çºæã®äžã€ã®éèŠãªå ·äœå
ã«åŸãã°ãååæ¿ïŒïŒã¯ãäžé£ã®çšç·ïŒïŒãšå¥ã®
äžé£ã®çšç·ïŒïŒãšã§æ§æãããæ Œå圢çŒã網暡æ§
ã®äž¡é¢ïŒïŒãä»äžãããŠãããçšç·ïŒïŒããã³ïŒ
ïŒã¯å¥œãŸããã¯äºãã«åçŽã§ããèããŠãããã¯
è€æ°åã®ç©åœ¢ããŒã¿ïŒïŒãç»æãããåçšç·ïŒïŒ
ããã³ïŒïŒã®åŽé¢ã¯ãããŒã¿ïŒïŒã®é£æ¥å¯Ÿããäž
æ¹åãžãšæ¬¡ç¬¬ã«ããŒããŒïŒæé¢åãæžå°ïŒããŠã
çšç·ã®é éšãç»æããç·ãšãªããæ³šæãã¹ãããš
ã¯ãå³é¢ã®çšç·ïŒïŒããã³ïŒïŒããæ¬çºæã®èª¬æ
ãç°¡ç¥åããããã«éåžžã«èªåŒµããŠæãããŠãã
ãšããããšã§ããã åæ¿ã®ææåœ¢ç¶ã¯ã該ææåœ¢ç¶ãåæ¿ã«ä»äžã
ã¹ãäŒå³ããããçªèµ·ã衚é¢ã«ãã€ããŒã©ãŒã«è©²
åæ¿ãéãããšã«ãã€ãŠéæããããæãããªã
ãã«ããã®ããŒã©ãŒã¯ãåæ¿ãäºå®äžç Žå£ããã
ãšãªããã®éåäœç©è³ªã®å€èгãå€åœ¢ããã«ååé
ã®å§åãããããããã«èšèšãããŠããããã
ã¯ãåæ¿ããã®è¡šé¢ã«åå¿æ§å±€ãæããŠããã®ã§
éèŠã§ãããããããŠãåæ¿ãå°ã¡æãããšã«ã
ããæäœãçµåããããšã®ã§ããªãåéšãæŽé²ã
ãããããªããã©ãã«ãªããšãã¬ã³å±€ã®æŽé²ã¯äº
å®äžãçµåèœåã®äœãåæ¿ãããããã§ãããã èæ ®ãã¹ãäž»ãªäºæã¯ãå¹³åºã®ãã€ã¢ã«ã«å ¥ã
ããšãå®è³ªäžè©Šéšè©Šæãšå®å šæ¥è§Šã®ç¶æ ã«ãªãã
ããªäž¡é¢ããã€åæ¿ãããããããšã§ãããããª
ãã¡ããããªãã¯ã¹ãšãã€ã¢ã«åºãšã®éã®é¢å士
ã®æ¥è§Šãæå°éã«ãã¹ãã§ãããæ¬çºæã®å¥ã®é
èŠãªå ·äœåã§ã¯ãåæ¿ã®äž¡é¢ã¯ãé«äœç¹åéãã
ã€ããã«åœ¢ã¥ããããŠããã æ¢è¿°ã®åŠããåçµä¹Ÿç¥åœ¢ã«è£œé ãããæäœã¯ã
æäœ5.0mgæ¯ã«0.1Mã®NaHCO3ïŒPH9.6ïŒã100ml
æ·»å ããããšã«ãã€ãŠåæ§æããããäžè¬ã«ãã
ã®çµåæ¹æ³ã¯ãïŒãïŒâã®è©²åžæº¶æ¶²ã«æ¹æäžãæ Œ
å圢ãªããçŒã網暡æ§ã®è¡šé¢ããã€åæ¿ãïŒã16
æéæ¥è§Šãããããšãå å«ãããæ¬¡ãã§ãæäœåž
溶液ã廿£ããåŸãåæ¿ããäºåºŠé£ç¶å®¹éã®
0.1M NaHCO3ïŒPH9.6ïŒããé žå¡©ç·©è¡å€å ¥ãé£å¡©
æ°Žããã³ã0.5ïŒ ã®ããŠã€ãŒã³ïŒTWEENïŒ20ã
嫿ããããé žå¡©ç·©è¡å€å ¥ãé£å¡©æ°Žäž0.3ïŒ ã®å·
ãïŒïŒãïŒâïŒçè¡æž ã¢ã«ããã³ã§æŽæµãããæŽ
ã«ãçµæ¶è³ªã®çè¡æž ã¢ã«ããã³ã§æŽæµãäžã€ãã©
ã€ã¢ã€ã¹äžã§åçµãããåŸãåçµä¹Ÿç¥ãè¡ããè
ããŠãã®åæ¿ã䜿çšã«äŸããŸã§ïŒãïŒâã§ä¿åã
ãã æ¬æçްæžäžã®èšèŒã¯ãèçæäœãçµåãããå
æ¿ã®è£œé ã«ä¿ãããã©ããæ¬çºæã®åæ¿ãã»ãšã
ã©ç¡éã®æ°ã®ããçœè³ªãåºå®ããã®ã«æçšã§ãã
ããšã¯åœæ¥è ã«æããã§ããããäŸãã°ã詊éšè©Š
æâåæ¿éã®æ¥è§Šå¢å ã«ãã€ãŠãäžèšããçœå®€ã
è©²åæ¿ãšã®çµåãå å«ãã詊éšã«çšããããšãã§
ãããããªãã¡ããããããçœè³ªã«ããžã³ãã·
ã³ãã¢ãã³å€ããã³ã¹ããã€ãã®åŠãè¬åã«å¯Ÿã
ãæäœïŒå€©ç¶ç£ç©äŸãã°ã€ã³ã·ãŠãªã³ããã³ä»ã®
ãã«ã¢ã³ã«å¯ŸããæäœïŒãªãã³ã«ãè¡æ¶²ãã®ä»ã®
äœæ¶²äžã«èŠåºããã代è¬ç£ç©ã«å¯Ÿããç¹ç°æ§é µçŽ
ãå å«ãããã äŸ ä»¥äžã®æé ãçšããŠåæ¿8000æã補é ãããå
åæ¿ãå ããæ¢è¿°ã®åŠãææåœ¢ç¶ã«ããããã«ã
ã¬ã¹åŠçãããèçæäœã¯åæ¿8000æã®ãããã«
40mgå¿ èŠã§ãã€ããããªãã¡ãåæ¿1000æã«ã€ã
ïŒmgã§ãããåæ§æããæäœã®ããçœè³ªå«éãã
0.1Mã®NaHCO3ïŒPH9.6ïŒ800mlã®æçµå®¹éã«ãã
ãŠ0.05mgïŒmlã«èª¿æŽãããæ¬¡ãã§ããã®å š800ml
ã®ç·©è¡å€å ¥ãæäœããæŽ©ãæ¢ãã©ã€ããŒãåãäž
ã«ã¯åæ¿8000æã®å ¥ã€ã1000mlã®ããèã³ãã«è£
å ¥ããããããŠããã®ã³ãã16æéäŸãã°äžå€ïŒ
ãïŒâã§å転ãããŠåå転ãµã€ã¯ã«ãéãåæ¿ã
ç·©åŸã«ã¿ã³ããªã³ã°ããããã«ããããã®åŸãæ¶²
ãã³ãããæµãåºã廿£ãããåæ¿ã¯ïŒã®åºå£
ãã©ã¹ã³ã«ç§»ããã åæ¿ãå·ãïŒïŒãïŒâïŒ0.1Mã®NAHCO3ç·©è¡
æ¶²ïŒPH9.6ïŒïŒã§äºåºŠç¶ããŠæŽæµãããã®åŸè©²
ç·©è¡æ¶²ãé€å»ãããæ¬¡ãã§ãåæ¿ãååºŠæŽæµã
ãããã®ãšãã¯ãå·ãç·©è¡å€ïŒ0.01Mã®ããé žã
ããªãŠã ã0.15Mã®NAClãPH7.4ïŒïŒãã€äºåºŠ
ã®é£ç¶æŽæµã§ãã€ããæ®çããç·©è¡å€ãé€å»ãã
åŸãåæ¿ãäžåºŠç®ã®æŽæµã«ä»ãããããã«ã¯ãå·
ããçè¡æž ã¢ã«ããã³æº¶æ¶²ïŒ0.3ïŒ ïŒïŒéãã€
äºåºŠã®é£ç¶æŽæµãçšããã æåŸã«ãåæ¿ããïŒmgïŒmlæ¿åºŠã®å·ãçµæ¶è³ªç
è¡æž ã¢ã«ããã³ïŒPHïŒïŒæº¶æ¶²ïŒéãã€äºåºŠã®é£
ç¶æŽæµã«ä»ããããã®å·¥çšã¯ãåæ¿äžã®ããçœè³ª
ã«ããçœè³ªç°å¢ãäžããç®çã§å®æœããããæ®ç
æŽæ¶²ãé€å»ããåŸãåæ¿ããäžæã®çŽã§å 匵ã
ããç¿ãªããã¯ãã¬ãŒïŒïŒÃ9inïŒã«ç§»ããã該
ãã¬ãŒã«ã¯å€«ã ã200mlã®çµæ¶è³ªçè¡æž ã¢ã«ãã
ã³æº¶æ¶²ãå ¥ããŠãã€ããç§»ãçµã€ãåŸãäžæã®
çŽã䜿ã€ãŠããããèŠã€ããç·©è¡å€ãå®å šã«é€å»
ããæ¬¡ãã§åæ¿ããã©ã€ã¢ã€ã¹äžã§30忥éã«å
çµãããã ãããåŸããã¬ãŒã®å 容ç©ãåçµä¹Ÿç¥ããã也
ç¥ããåæ¿ãåãåºãããããç®æ¢ããã容åšå
ã§è²¯èµããã (1) å¯Ÿç §è¡æž ã®èª¿è£œ å ç«æž¬å®ããæå³æ·±ãè³æãåŸãããã«ãé°æ§
ãšéœæ§ã®å¯Ÿç §è¡æž ãçšæããŠæå®ã®è©Šéšè©Šæãšé©
宿¯èŒããå¿ èŠãããããããå¯Ÿç §ç©ã®èª¿è£œã以
äžã«è©³è¿°ããã é°æ§ã®å¯Ÿç §ã¯ãäŸãã°æŸå°å ç«æž¬å®æ³ã«ããã
èçã¢ã³ãã²ã³ã«ã€ããŠè©Šéšãé°æ§ãšããã€ã人
è¡ããããã補é ãããèããŠãèçã¢ã³ãã²ã³
ã«é¢ãæããã«é°æ§ã§ããåãŠãããã«ã5Mã®
CaCl2ãè¡ããã200mlã«ã€ãCaCl2溶液0.75mlã®
åºæºã§æ·»å ããŠåå¡ãèªåŒãããæ¬¡ãã§ãåå¡ã
圢æãããŸã§ãã®è¡ããããæ°ŽæµŽäž37âã§æž©çœ®ã
ããåå¡ããè¡ããããŠããããæ¬¡ãã§â20âã§
åçµããŠå°ããšã12æé貯èµãããæ¬¡ãã§ããã®
è¡ããããŠããããïŒãïŒâã§èè§£ãããè¡æž ã
éãããããè¡æž ãéåºŠã«æ¿ã€ãŠãããããã
ããäŸãã°ïŒãïŒâã§30åé9000rpmã®é å¿åé¢
ã«ããæž æŸåããããšãæãŸãããããããªãã ã·ãªã«äŸãã°ãšã¢ããžã«ïŒAEROSILïŒâ380
ãè¡æž ïŒåœã20ïœå ããïŒæéå®€æž©ã§æ··åããŠ
ãªããããã€ã³ãé€å»ãè¡æž ãå®å®åããããæ¬¡
ãã§ããã®æ··åç©ãé å¿åé¢ã«ãããæ²æ®¿ç©ãå»
æ£ãããããææãªããé©åœãªéææ®µã«ãã€ãŠ
éããŠã·ãªã«ãé€å»ããããšãã§ããã æ¬¡ãã§ãäžæŸã¿æ¶²ãæŽã«ãäŸãã°ããªãã¢
ïŒMILLIPOREïŒèãªããããŒã ïŒHORMïŒèã
ãã«å€åçãé£ç¶çã«äœäžãããããã§éåŠç
ããããããã¯0.45ãã¯ãã³ã®èã§ããããã®
0.45ãã¯ãã³ã®èã§éããåã«ããããªãŠã ã¢
ãžãïŒNaN3ïŒã0.1ééïŒ ã®æ¿åºŠã§äžèšäžæŸã¿æ¶²
ã«æ·»å ãããæ¯çã§åšç¥ã®åŠãããããªãŠã ã¢ãž
ãã¯é²è å€ãšããŠäœçšããã0.45ãã¯ãã³ãªãã
ãã以äžã®å€åçãã€ã«ã¿ãŒã«ããæåŸã®é
ã¯ãç¡èã®è£ 眮ããã³æè¡ãçšããå±€æµç°å¢ã§è¡
ãã¹ãã§ããã æ¬¡ãã§ããã®ç¡è溶液ã¯ãå ç«æž¬å®çšè©Šè¬ç®æ¢
ãæº¶åšã«å°åãããããŸã§ïŒãïŒâã§è²¯èµããã
ããæç€ºãã100åã®è©Šéšã»ããã«ã¯ã7.5mlã®é°
æ§å¯Ÿç §è¡æž ãäŸäžãããã éœæ§å¯Ÿç §è¡æž ã¯ãèçã¢ã³ãã²ã³è©Šéšã§éœæ§ãš
åºãè¡æ¶²ãŠãããããã®è¡ããããåç³ç°åãã
ããšã«ãã補é ãããåéœæ§ãŠãããããïŒïŒ 詊
æãæ¡åããããããäžç·ã«ããŒã«ããŠè©ŠéšããŒ
ã«ãšãããå ãããã®è©ŠéšããŒã«ã60âã§10æé
ç±åŠçããŠè©Šæäžã®èçèªçºç©è³ªãå šãŠäžæŽ»æ§ã«
ããã該ããŒã«ã宀枩ã«å·åŽãããšããã¢ã³ãã²
ã³æŽ»æ§ãä¿çãããŠããããšãæ€æ»ãã¹ãäžéšå
ãåãåºããCEPæè¡ã䜿ã€ãŠåºæºæäœã«å¯Ÿã
滎å®ãããæ¬¡ãã§ã詊éšããŒã«ã«ãè¡æž ïŒåœã
20ïœæ¿åºŠã®ã·ãªã«ãæ·»å ããããã®è¡æž ãæ¬¡ãã§
ïŒæéå®€æž©ã§æ¹æãããã®åŸãããïŒãïŒâã§30
åé9000rpmã®é å¿åé¢ã«ããããæ²æ®¿ç©ã¯å»æ£
ããã åŸãããäžæŸã¿æ¶²ããCEPã䜿ã€ãŠå¯Ÿç §åºæº
æäœã«å¯Ÿã滎å®ãããããå䟡ãç³åã®ãªãã¬ã
ã«ã®ãŸãã§ããã°ãå šè¡æž ãŠãããã®ç·éãäžç·
ã«ããŒã«ãäžã€ãããã詊éšããŒã«ã§è¿°ã¹ããšå
ãããã»ã¹ã«ä»ãããšãã§ããã äžç·ã«ãã詊éšããŒã«ãšäž»èŠããŒã«ã¯ãæ¬çºæ
ã®å ç«æž¬å®ã§éœæ§å¯Ÿç §è¡æž ã«é¢ãæé©çµæãåŸã
ããããã«ååéã®é°æ§å¯Ÿç §è¡æž ã§åžéããã奜
ãŸããã¯ãæ¬çºæè©Šéšã§ã®éœæ§å¯Ÿç §è¡æž ã®èªã¿ã
åžå 床åäœÃ1000ã§2000ããé«ãããã¹ãã§ã
ãããã®åžéããéœæ§å¯Ÿç §è¡æž ãæ¬¡ãã§ãæ¢è¿°ã®
åŠããé£ç¶ç¯å²ã®äœäžå€åçãçšããé©åœãªææ®µ
ã§éããã0.45ãã¯ãã³åã¯ãã以äžã®èã§æ
çµçã«éããåã«ã0.1ééïŒ ã®ãããªãŠã ã¢
ãžããæ·»å ãããé°æ§ããŒã«ã®ãšãã®ããã«ãã
ã®æåŸã®éã¯ãå±€æµç°å¢äžç¡èæ¡ä»¶äžã§è¡ãã¹
ãã§ããã åŒ±éœæ§å¯Ÿç §è¡æž ã¯ãåŒ·éœæ§å¯Ÿç §ãé°æ§è¡æž ã§åž
éããããšã«ãã補é ããããšãã§ãããåŒ±éœæ§
è¡æž ã®èªã¿ã¯ãæ¬çºæã®è©Šéšã§æž¬å®ãããšã600
ã1000ã§ããã¹ãã§ãããæ¬çºæã®å¥œãŸããå ·äœ
åã®100å詊éšã»ããã«ã¯åŒ·éœæ§å¯Ÿç §2.5mlãšåŒ±éœ
æ§å¯Ÿç §2.5mlãåãä»ããããã  ãã®ä»ã®è©Šè¬ããã³è£ 眮 0.5ã€ã³ããããããã«å€§ããªããªãã¡ãçŽåŸ
0.50ã€ã³ãã®åæ¿ã«ã€ãåã寞æ³ã®çŽåŸãæãã
200ãªãããã以äžã®ã¬ã©ã¹è£œå¹³åºãã€ã¢ã«ïŒäœ¿
ãæšãŠïŒãåãã€ãããããããããã€ã¢ã«ã®ã
ã¡100åã¯ã詊éšã®åãã®æž©çœ®ããã³æŽæµçšã«äœ¿
ãããæ®ã100åã¯ãåŸã®ãé µçŽ åºè³ªãšã®æž©çœ®ã«
çšãããããåŸã€ãŠããã®è©Šéšã»ããã«ããã
100åã®æž¬å®ãåæã«è¡ãããšãã§ããã 以äžã«è©³è¿°ããåŠããäžæº¶æ§åºäœåæ¿äžæãã
ãã100åã®ãã€ã¢ã«åã ã«å ¥ããåã«ã銬ã°ã
ããªã³è©Šéšè©Šæåžéå€ã0.05mlãã€åãã€ã¢ã«ã«
å ¥ããããã®å·¥çšã¯ã詊éšè©Šæãšäžæº¶æ§åæ¿ãšã®
åãã®æž©çœ®ã§éç¹ç°æ§åå¿ãæé€ããäºåææ®µãš
ãšãããåæ¿äžã«è¢«èŠãããæäœã¯ç²Ÿè£œãããŠã
ããèçã¢ã³ãã²ã³ãšé«åºŠã«åå¿ããããã©ãã
äººè¡æž åã¯è¡ãããäžã«ã¯ã銬ã°ãããªã³ãã®ã
ã®ãšåå¿ãåŸèããŠåæ¿ãšé µçŽ æšèçµåäœãšã®é
ã«æ©ã圢æããŠæ¬äŒŒéœæ§åå¿ãããããããç©è³ª
ãææååšãããå ã®å·¥çšã§æ·»å ãã銬ã°ãããª
ã³ã¯ãã®ç©è³ªãšçµåãããã®ããåæ¿ãšã®åå¿ã¯
èªç±ã§ãªããå ã«é瀺ããåŠããåæ¿äžã«è¢«èŠã
ããæäœã¯ãäººè¡æ¶²ããéããèçé¢é£ã¢ã³ãã²
ã³ã§éЬãå ç«åããããšã«ããç£çããããæ¬çº
æã®100å詊éšã»ããã«ã¯ãããé žå¡©ç·©è¡å€å ¥ã
é£å¡©æ°ŽïŒPBSïŒã«éЬã°ãããªã³330mgãæº¶è§£ããŠ
ãªã溶液5.5mlãåãã€ããããã  åºè³ªã®è£œé 詊éšã®é µçŽ åå¿çšã«éžãã åºè³ªã¯ïœâãããã
ãšãã«ãã¹ããšãŒãã§ããããã®ãã®ã¯ãçé žã
ããªãŠã ç·©è¡å€ã«ïŒmgïŒmlã®æ¿åºŠã§æº¶è§£ãããã
ç·©è¡å€ã®æ¿åºŠã¯0.028ã¢ã«ã®çé žãããªãŠã ãã
ã³0.001ã¢ã«ã®ãã°ãã·ãŠã ïŒPH9.8ïŒã§ãã€ãã ãã®æç¹ã§æ³šæãã¹ãããšã¯ãäžã®è¡šã«ç€ºã
åŠãä»ã®åºè³ªãé©åœãªPHç·©è¡å€ãšå ±ã«çšããããš
ãã§ãããšããããšã§ããã
ååšãæ€åºããæ¹æ³ã«ä¿ããç¹å®ããã«ãæ¬çºæ
ã¯ãèèçã®ååšãæ€åºãã¹ãé µçŽ ã§æšèããã
æäœãçšããŠã¢ã³ãã²ã³ãšåå¿ãããããšãå å«
ããèèçã«é¢é£ããã¢ã³ãã²ã³ã®å ç«æž¬å®ã«ä¿
ãã ãèèã®ççããæå³ããèèçã¯ãè管ãæ
æãåã¯éå¡ããããšã«ãããããŒã«ã¹æ§èèç
ã«ã¯ïŒçš®é¡åšã€ãŠãäžæ¹ãä»ã®ãã®ããæœäŒæã
é·ããšèãããããåŸåãæ£è ãèèçã«ããã
æ¢ç¥ã®éçµå£çæŽé²ãæãããšãããã®èèçã¯
ãè¡æž æ§èçããšãããããããæ£è ãæ¢ç¥ã®é
çµå£çæŽé²ãæããçµå£çã«èèçã«ããã€ãŠã
ãã°ãããã¯ãäŒææ§èçããšåŒã°ãããããã
ãªãããæç®çã§æ å ±æäŸãããŠããããã«ãäž¡
è ã¯æœäŒæãéãªãåãããã®äžãäŒææ§èçã
ã§ãã€ãŠãéçµå£çã«ãããããšããããæè¬
ãè¡æž æ§ãèçã§ãã€ãŠãçµå£çã«ãããããšã
ãããããããŠãèèçã«ã¯å°ããšãïŒçš®ã®éã€
ãåªä»ã«ãã€ãŠåŒãèµ·ãããäºã€ã®ã¿ã€ãããã
ããã«æãããããããããèå¥ããã®ã«çšèª
ãè¡æž æ§èçãããã³ãäŒææ§èçããçšããã¹
ãã§ãªããåŸã€ãŠããäŒææ§èçãã«æãè¿äŒŒã
ãã¿ã€ãã®èçã«ã¯ãèïŒèïŒçããçšããã¹
ãã§ããããŸããè¡æž æ§èçãã«æãè¿äŒŒããŠã
ããã®ã«ã¯ïŒèïŒèïŒçããçšããã¹ãããšã
瀺åãããŠããã æ¬æçްæžã«èšèŒã®ãäŸãã¯èèçã«é¢é£ãã
ã¢ã³ãã²ã³ã®æ€åºã«æåãããèããŠãè¡æž æ§è
çã«æãè¿äŒŒããèèçã«ããã€ãŠããæ£è ã¯ã
ãããã©ããªçç¶ã§ããããã°ãã°è¡æ¶²äžã«äžèš
ã¢ã³ãã²ã³ãæããŠããããã®æç¹ã§æ³šæãã¹ã
ããšã¯ãèèçåã¯ä»®å®ãããèèçã«é¢é£
ããã¢ã³ãã²ã³ã®ååšã枬å®ããããã®ä¿¡é Œãã
ãæ¹æ³ããªããšããããšã§ãããããããŠãæ¬æ
现æžäžã®äŸã¯èèçã«é¢é£ããã¢ã³ãã²ã³ã®å
åšãæ€åºããããšã«æåãããããããªãããå¥
ã¿ã€ãã®èèçã«é¢é£ããã¢ã³ãã²ã³ã§ãã€ãŠã
ãããäžåºŠåå®ããããªããã®ã¢ã³ãã²ã³ã®ååš
ãæ€åºããã®ã«æ¬çºæã®æ¹æ³ã䜿çšããåŸãªãç
ç±ã¯ãªãã ãè¡æž æ§èçããªããèèçã«ããããšãç¡
èŠããããšã®ã§ããªãèé ·ãªèšåºåé¡ãåµåºã
ããèããŠãåé¡ãèé ·ãªããæ ã«ãèèçãæ€
åºããããã®è©Šé𿹿³ãçš®ã éçºããããããã
è©Šéšæ¹æ³ã«ããã¯ãã»ã¢ãŠãã¿ã©ããŒïŒMicroâ
OuchterlonyïŒãå ç«æ¡æ£ãè£äœçµåãå ç«é»æ°
æ»²éæ³ïŒImmunoelectroâosmophoresisïŒããã
åéããã³ãã åéæå¶ãé»åé¡åŸ®é¡ããªãã³ã«
åºçžæŸå°å ç«æž¬å®ãå å«ãããããããã®è©Šéšæ¹
æ³ã¯åã ãããªãã€ãã·ãŠã»ã¡ãžã«ã«ã»ããªãã³
ïŒBritish Medical BulletinïŒã1972ãVol.28ãNo.
ïŒïŒãŠã€ã«ã¹æ§èçïŒãP138ã141ã«è©³è¿°ãããŠ
ããã®ã§å¿ èŠã«å¿ããããåç §ããããã å ç«é»æ°æ»²éæ³åã¯é黿°æ³³åæ³ïŒCEPïŒ
ã¯ãèèçã«é¢é£ããã¢ã³ãã²ã³ïŒä»¥äžææèç
é¢é£ã¢ã³ãã²ã³åã¯åã«èçã¢ã³ãã²ã³ãšåŒç§°ã
ãïŒãšãã®æäœïŒä»¥äžåã«èçæäœãšåŒã¶ïŒãæ€
åºããè¿ éäžã€ç°¡åãªæ¹æ³ãäŸäžãããããããª
ããããã®æè¡ã¯ãäŸãã°è£äœçµåã«æ¯ã¹è¥å¹²æ
床ã«ãããŠå£ããèããŠããã®äž»ãªå©ç¹ã¯ãïŒæ
éå ã§è©Šéšãå®äºããããšããããšã§ããããã
ãããªãããæåºŠã¬ãã«ãäœãããã«ãCEPã¯
é£åå»è¬ç®¡çå±ïŒthe Food and Drug
AdministrationïŒã«ãã€ãŠãã¯ãèªå¯ãããŠããª
ãã æŸå°å ç«æž¬å®æ³ïŒRIAïŒã¯ã詊éšãè¡ãã®ã«å¿
èŠãªè£ çœ®ãæ¯èŒçè€éãç¹æ®ãªé«äŸ¡è£ 眮ã§ããã°
ããã§ãªããæŸå°æ§åäœå çŽ ïŒã©ãžãªã¢ã€ãœããŒ
ãïŒãåæ±ãéå³éãªäºé²çãèŠæ±ãããã®ã§ã
ãã®æè¡ãæ¥åžžèšºæç®çã«é©çšããããšã¯è¥å¹²å¶
éããããã®ãšä¿¡ããããã®äžãã©ãžãªã¢ã€ãœã
ãŒãæšèã¯éå€§ãªæœåšçå¥åº·å±éºãåããç£èŠã
ãã³ååãšãã«ã®ãŒå§å¡äŒïŒAtomic Energy
CommissionïŒã®ïŒäœ¿çšããã³è£œé ã«å¯ŸããïŒå
èš±ãå¿ èŠãšããäžã€å»æ£ç©åŠçåé¡ãåãããã«
ããããããããã®æè¡ã¯ä»æ¥ãå ç«æž¬å®ããã®
ã«ããªãããå®çããŠããã ç¡è«ãå ç«åŠçæ¹æ³ã¯ãå šãŠã®æäœããã³ã¢ã³
ãã²ã³ã®äž»èŠç¹æ§ããªãã¡ç¹å®ã®è£äœã¢ã³ãã²ã³
åã¯æäœãšã®åå¿æ§ã«äŸæ ãããããããŠããã
æäœããã®ã¢ã³ãã²ã³ã嫿ããè¡æž ã«æ·»å ãã
ããªããæäœãšã¢ã³ãã²ã³ã¯é¯åœ¢æãèããŠæº¶æ¶²
ããæåºããããšãã§ãããã»ãšãã©ã®åèšè©Šéš
æ¹æ³ã«ãããŠããã®ç°¡åãªäºå®ãå©çšããããšã«
ãããäººè¡æž äžã®ã¢ã³ãã²ã³ã®ååšãæ€åºãã
ãã ãããŸã§ãæšèæäœãçš®ã ã®ã¢ã³ãã²ã³ãåå®
ããããã«çšããããŠããããããç¹å®ã®ã¢ã³ã
ã²ã³ã«å¯Ÿãç¹ç°æ§ã§ããããšãããã€ãŠããæäœ
ãã該æäœç£æ§ã®ããåºæ¿ãããå¯äž»åç©ã®è¡æž
åã¯è¡ãããã®ã°ãããªã³éšåããåé¢ãããª
ãããã®æäœã¯æ¢ç¥æ¹æ³ã«ãã€ãŠæšèããããšã
ã§ããããã®æäœããæšèå€äŸãã°äžè¿°ã®åŠãã©
ãžãªã¢ã€ãœããŒãåã¯è¢å è¬åã®ãããªç©ççã«
æ€åºã§ããç©è³ªãšçµåãããããšã«ãããæäœã®
ååšãæ€åºããããšãã§ãããããããŠãæšèæ
äœã蚺æã«çšãããšãã察å¿ããã¢ã³ãã²ã³ãæ
ã調補詊éšè©Šæäžã«ååšããã°ã該æšèæäœã¯ã
ã®ã¢ã³ãã²ã³ãšçµåããèããŠè©Šéšè©Šæäžã®è©²æš
èæäœãæ€åºããããšã«ããã¢ã³ãã²ã³ã®ååšã
確èªããããã ãã®èšºæçšæšèæäœã¯ããããæ€åºãææãã
ãã¢ã³ãã²ã³ãšã ãåå¿ã該ã¢ã³ãã²ã³ãšã¯å šã
ç°ãªãããã»ã©éèŠã§ãªãä»ã®è¿çžã¢ã³ãã²ã³ãš
çžäºåå¿ããªãããã«ååç¹ç°æ§ã§ããã¹ãã§ã
ããããããŠããããªãå ç«æž¬å®ã«ãããŠããæ
äœã®çµŠæºããã³æäœã®ç£çæ¹æ³ãããããå šãèš
ççã§ãããšããããšã¯æããã§ããã åè¿°ã®åŠããèèçã«é¢é£ããã¢ã³ãã²ã³ãæ€
åºããæ¹æ³ã®äžã€ã«åºçžæŸå°å ç«æž¬å®æ³ãå å«ã
ããããã®æé ã¯ç±³åœç¹èš±ç¬¬3867517å·ã«é瀺ã
ããŠããã該ç¹èš±ã«é瀺ãããåŠããæŸå°å ç«æž¬
å®ãè¡ãéã該枬å®ã«çšããããæåœ¢ããªã¹ãã¬
ã³ã®ããŠãŒããŠãšã«ãªããã€ã³ãµãŒããå ãæäœ
ã§è¢«èŠããããããã¯ã被èŠãã¹ãè©²éšæãæäœ
溶液ãšäžç·ã«æž©çœ®ããããšã«ãã€ãŠéæãããã
ãã®åŸãæªç¥è©Šæãããã®æäœè¢«èŠããããŠãŒã
ãŠãšã«ãªããã€ã³ãµãŒããšå ±ã«æž©çœ®ããŠæäœãšè©²
詊æäžã«ååšããã¢ã³ãã²ã³ãšãåå¿ããããã
ãŠãŒããŠãšã«ã¯æ¬¡ãã§æŽæµãããæŽã«æŸå°æ§åäœ
äœïŒ©â125ã§æšèãããæäœãšå ±ã«æž©çœ®ãããã
ååºŠã®æŽæµã«ãã€ãŠãæªçµåã®æšèæäœãå šãŠå
ãé€ããããããããŠã詊éšè©Šæäžã«ã¢ã³ãã²ã³
ãååšããå Žåãããªã¹ãã¬ã³ã®ããŠãŒããŠãšã«
ïŒãªããã€ã³ãµãŒãïŒãæäœãã¢ã³ãã²ã³ããã³ïŒ©
â125æšèæäœãããªããµã³ãã€ããã圢æãã
ããæ¬¡ãã§ããã®ïŒ©â125æšèæäœããåºãæŸå°
ç·ãèšæ°åšã«ãã€ãŠæž¬å®ããå¯Ÿç §ãšæ¯èŒãããã ãŸããæŸå°å ç«æž¬å®ãè¡ãéã眮æããªã¹ãã¬
ã³äŸãã°ã€ãœããªã·ã¢ãã¹ãã¬ã³ãã°ã©ãããã
ãããªããããã«ãªã«ãšãã¬ã³ã®åæ¿ãæçšã§ã
ãããããšãé瀺ãããŠããããã®ããªã¹ãã¬ã³
ã¯ãããçœè³ªãšå ±æçµåããŠçç©è©Šéšã«æçšãªè©Š
è¬ãããããããããçœè³ªåå¿æ§åºã®ç¹æ®èšèšè¡š
é¢ããã€äžæº¶æ§ç©è³ªã§ãããæè¿ãæäœãã©ãžãª
ã¢ã€ãœããŒãåã¯è¢å è¬åã§æšèããããšã«åã€
ãŠä»£ãéèŠãªæ¹æ³ãéçºããããããã¯ãæäœã
é µçŽ ã§æšèããæ¹æ³ã§ããããã®æé ã«ã€ããŠ
ã¯ãç±³åœç¹èš±ç¬¬3654090å·ããã³å第3791932å·ã«
èšèŒãããŠããããŸããã¯ãªãã«ã»ã±ãã«ã«ã»ã¢
ã¯ã¿ïŒClinika chemical ActaïŒã48ïŒ1973ïŒã15
ã18ã«ã¯ããµã³ãã€ããæ³åã¯ç«¶åããããã«ã
ãã¢ã«ãã¢ããšããããã€ã³ã®é µçŽ çµååŒå ç«æž¬
å®ãé瀺ãããŠããã é µçŽ æšèã¯ãæŸå°å ç«æž¬å®ã«æ¯èŒããŠããã€ã
ã®éèŠãªå©ç¹ãæããŠãããäŸãã°ãæçµæ··åç©
äžã®é µçŽ æšèååã¯å šãŠãèªã¿åãã«åäžããã
仿¹ãæçµæ··åç©äžã®ã¢ã€ãœããŒãååã¯ãèªã¿
åãã®éããå°å²åãã厩å£ããèããŠè©²å²åã®
ã¿ãæŸå°å ç«æž¬å®ã«åäžããã«ãããªãããŸãã
é µçŽ æšèã§ã¯ãæ€åºå¯èœãªæçµçæç©ã圢æãã¹
ãå€ãã®åºè³ªååãæ»æããããšã«ãããå šãŠã®
æšèãèªã¿åãã«ãäŸãã°100000åïŒminãŸã§å
埩åäžãããããããæåºŠã倧巟ã«é«ãããã
ãïŒã¢ã€ãœããŒãæšèååã¯èªã¿åãã®éïŒåºŠã
ã厩å£ãããã®åŸã¯åäžããªããªããé µçŽ æšè詊
è¬ã¯ä¿å寿åœãé·ããïŒã¢ã€ãœããŒãæšè詊è¬ã¯
çµ¶ãã厩å£ãã€ã€ããããã®ä¿å寿åœåé¡ã¯èé ·
ã§ããããŸããé µçŽ æšè詊è¬ã®äœ¿çšã«é¢é£ããå¥
康å±éºã¯æå°ã®ãã®ã§ããããã¢ã€ãœããŒãæšè
ã®å Žåããã¯é倧ã§ãããæŽã«ãŸããé µçŽ æšèã«
ããå ç«æž¬å®ã¯ãç°¡åã§äžã€æ¯èŒçå®äŸ¡ãªèªã¿å
ãè£ çœ®ãçšããããšãã§ãããïŒã¢ã€ãœããŒãã«
ããå ç«æž¬å®ã®éŠå°Ÿã¯ããããšèããçžéããŠåŽ©
å£ã®æ€åºå¹çã«äŸæ ãèããŠãŸããããããŠé«äŸ¡
ã«ã€ãæ€åºè£ çœ®ã®æ§èœã«äŸæ ããã 人ã®è¡æ¶²äžã«èçã¢ã³ãã²ã³ãèŠåºãããšã¯ã
èèçã®èšåºæŽãååŸããããšãšåçã§ã¯ãªãã
ããããªãããçš®ã ã®ç ç©¶ã«ãã€ãŠãèçã¢ã³ã
ã²ã³ã«é¢ãéœæ§ãšåºãè¡æ¶²ãæ£è ãå容ãããšã
èçææã®çºç ãé«ããšããã€ãããã°ãã°ãè¡
æ¶²ã»ã³ã¿ãŒããå ¥æãããç¹å®ã®è¡æ¶²ãŠãããã
䜿çšãããåŠãããæ¯èŒççæéã§æ±ºå®ããã°ãª
ããªãã®ã§ãèèçã®ã¹ã¯ãªãŒãã³ã°ãã¹ãïŒéž
å¥è©ŠéšïŒãé«äŸ¡ãªè£ 眮ãå¿ èŠãšããããšãªã髿
床ãè¿ éäžã€å®¹æã«è¡ãããšã¯ããããŠéèŠã§ã
ããèèçã«é¢é£ããã¢ã³ãã²ã³ã®æ€åºã«åŸæ¥çš
ããããçš®ã ã®è©Šéšã¯æãçšåºŠæºè¶³ãããŠããã
ãã©ãããããããã¯å šãŠäžã€åã¯äºã€ä»¥äžã®æ¬
ç¹ãæããã æ¯å¹Žèèçã«ãã€ãŠäœå人ãã®äººãæ»ã«äžã€å ¥
é¢ããŠãããé·ãéããã®ç æ°ãæå¶ããéµã¯ã
äžççã«æå¹ã§ãããŸã容æäžã€æ¥åžžçã«è¡ãã
ãè¡æ¶²éžå¥æè¡ã§ãããšèããããŠãããæ¢è¿°ã®
ããã«ãããã€ãã®æè¡ã仿¥å容ãããŠããã
ãã©ãããããã¯æ¯èŒçäœæåºŠã§ãããç¹éäžã€
æéã®ãããæé ãå¿ èŠãšããã»ãšãã©ã®ãã¢ã
ããžãŒã©ãïŒhemotology labïŒã«ãããŠå®¹æã«
ã¯å©çšãåŸãªãè€éãªè£ 眮ã䜿çšããã°ãªããã
æã¯ãéåžžã«äžå®å®ã§æè¿ãã«ã¯ä¿å šãåŸãªã詊
è¬ãçšããã°ãªããªããããæ ããããã®æè¡ã¯
çæ³çãªè©Šéšã®èŠæ±ãæºãããã®ã§ã¯ãªãã€ãã æŠæ¬ããã«ãæ¬çºæã¯ãããµã³ãã€ãããåç
ãçšããèçã¢ã³ãã²ã³ã®çŽæ¥çå ç«æž¬å®ãå å«
ããããã®æž¬å®ãè¡ãã«éããæ€åºãããšããã¢
ã³ãã²ã³ãããããšåå¿ããæäœå±€ã®éã«ã¯ã
ããäžæ¹ã®æäœå±€ã¯é µçŽ ã§æšèãããŠãããä»ã®
å±€ã¯äžæº¶æ§éšæã«å ±æçµåããŠãããé µçŽ ã¯ãå
åŠåºè³ªã«ããããããã該åºè³ªã¯é µçŽ è§Šåªã®ååš
ã§ååŠå€åãèµ·ãããŠåå¿çæç©ã圢æãããè
èçã®æç¡ã¯ããã®åå¿çæç©ãå®éããããšã«
ãã€ãŠæ±ºå®ãããã æ¬çºæã®å¥ã®æ§çžã«åŸãã°ãèèçã®æ€åºãã
ããªããã»ãããäŸäžãããããã®æ€åºã»ããã®
äžå€§è©Šè¬ã¯ãèèçã«é¢é£ããã¢ã³ãã²ã³ãšåå¿
ããæäœãçµåãããäžæº¶æ§éåäœåºäœãé µçŽ ã§
æšèãããèçæäœè©Šè¬ããã³ãé µçŽ ã®è§Šåªç圱
é¿äžååŠçã«å€åããŠæ€åºå¯èœãªæçµçæç©ã圢
æããããšã®ã§ããé µçŽ åºè³ªã§ãããéžã°ããå ·
äœåã§ã¯ãçµåäœã®é µçŽ ãã¢ã«ã«ãªæ§ãã¹ãã¢ã¿
ãŒãŒïŒããé žé µçŽ ïŒã§ãããé µçŽ åºè³ªãïœâãã
ãããšãã«ãã¹ããšãŒãã§ãããäžèšæ€åºã»ãã
ã«ã¯ãŸããèçã¢ã³ãã²ã³ã«å¯Ÿãé°æ§ãåŒ±éœæ§ã
ãã³åŒ·éœæ§ã®è¡æž ãå å«ããå¯Ÿç §è¡æž ã詊éšè©Šæ
âäžæº¶æ§éåäœåºäœéã®éç¹ç°æ§åå¿ã®é »åºŠãæ
å°éã«ãã詊éšè©ŠæçšéЬã°ãããªã³æ·»å å€ã®æº¶
æ¶²ãé µçŽ åºè³ªæº¶æ¶²ã®PHãåå¿ã«æé©ãªç¯å²ã«ä¿æ
ãã¹ãäŒå³ãããç·©è¡æ¶²ããªãã³ã«æž©çœ®ã§çšãã
ããå°éã®è©Šè¬âäžæº¶æ§éåäœåºäœéã®æ¥è§Šãé«
ããããã«èšç®ããã寞æ³ã®è€æ°åã®ãã€ã¢ã«
ïŒå°ã³ãïŒãå«ãŸãããé µçŽ âé µçŽ åºè³ªç³»ãšããŠ
ã¢ã«ã«ãªæ§ãã¹ãã¢ã¿ãŒãŒããã³ïœâãããããš
ãã«ãã¹ããšãŒããçšãããšããç·©è¡æ¶²ã¯
Na2CO30.028Mããã³Mgã0.001Mã®æ°Žæº¶æ¶²ã§ã
ãã æ¬çºæã®å¥ã®æ æ§ã«ãããŠãã¢ã«ã«ãªæ§ãã¹ã
ã¢ã¿ãŒãŒã®åŠãå®èœé µçŽ ãšååŠçã«çµåãããå
ç«åŠäžæŽ»æ§ãªç²Ÿè£œèçæäœãå ç«æž¬å®æ³ã«çšãã
ããã¹ãäŸäžãããã æ¬çºæã®æŽã«å¥ã®æ æ§ã«ãããŠãåæ¿æ§äžæº¶æ§
éšæãªãããããªãã¯ã¹ãå ç«æž¬å®æ³ã«çšããã
ãã¹ãäŸäžãããããã®ãããªãã¯ã¹ã¯ãã®è¡šé¢
å±€ã«ãããçœè³ªãšåå¿ããè€æ°åã®åºããäžæ§ã«
ã°ã©ãããããŠããã粟補ãããèçæäœã¯ãã®
åå¿åºã«å ±æçµåããŠèçæäœã®å€é¢å±€ãããã
ãã å ç«æž¬å®æ³ã«ãããŠãäžèšã®äžæº¶æ§éšæã¯å¹³åº
ã®ãã€ã¢ã«ã«å ¥ããããäžã€æ¶²äœè©Šéšè©Šæã§çšã
ãèŠãããã詊éšãåçŸæ§ã§ããããã«ã¯ã詊éš
詊æäžã®ã¢ã³ãã²ã³ãæäœã§è¢«èŠãããåæ¿ã®è¡š
é¢å šäœã«æŽé²ãããŠããããšãéèŠã§ãããå¹³ã
ãªè¡šé¢ããã€åæ¿ã®äœ¿çšã¯æåºŠæå€±ã«åž°ãããšã
çºèŠããããåŸã€ãŠãæ¬çºæã®åæ¿ã®äž¡é¢ã¯ãå
æ¿é¢âãã€ã¢ã«åºã®æ¥è§Šãå°ãããããã«æãäž
æŽåœ¢ç¶ã«å€åœ¢ãããŠãããéžã°ããäžã€ã®å ·äœå
ã§ã¯ãæäœãçµåãããã«å ç«ã€ãŠåæ¿ããã¬ã¹
ã«éãããšã«ããããã®äž¡é¢ãçŒãç¶²æ§ãªããæ Œ
å圢ã«ãããã ããæ ãæ¬çºæã®äž»èŠç®çã¯ãäœæ¶²äžã®èèç
ã«é¢é£ããã¢ã³ãã²ã³ã®æç¡ãè¿ éãç°¡åãæ£ç¢º
äžã€æ¥åžžçã«æ±ºå®ããããããªèèçã®æ€åºãã
ãããã³ãã®äœ¿ç𿹿³ãæç€ºããããšã§ããã æ¬çºæã®å¥ã®ç®çã¯ãæ¯èŒçæªçãªäººãèèç
ã«é¢é£ããã¢ã³ãã²ã³ã®é«æåºŠåçŸæ§å ç«æž¬å®æ³
ãæ¥åžžåºæºã§å®æœããã®ã«é©åããåäœå詊éšã»
ãããæäŸããããšã§ããã æ¬çºæã®å¥ã®ç®çã¯ãå ç«æž¬å®æ³ã宿œããã«
éãæé äžã®èª€ããæå°éã«ãã¹ãäŒå³ãããåŠ
äžã®è©Šéšã»ããã«ããŠãå¿ èŠãªè©Šè¬ãåå¿å®¹åšç
ãå šãŠã詊éšã®ç²ŸåºŠããã³æåºŠãæé©ã«ãã¹ãäŒ
å³ããã圢ã§å ¥ãã該詊éšã»ãããæäŸããããš
ã§ããã æ¬çºæã®å¥ã®ç®çã¯ã詊éšã®é宿œãããçš®ã
ã®å·¥çšãæšæºåããããã«äŒå³ããã詊éšã»ãã
å ¥ãå®éšå®€è£ 眮ãæäŸããããšã§ããã æ¬çºæã®æŽã«å¥ã®ç®çã¯ã詊éšè©Šæã®æ¯èŒåºæº
ãšããŠãèçé¢é£ã¢ã³ãã²ã³ã«å¯Ÿãé°æ§ãåŒ±éœæ§
ããã³åŒ·éœæ§ã®å¯Ÿç §è©Šæãå«ã詊éšã»ãããæäŸ
ããããšã§ããã æ¬çºæã®å¥ã®ç®çã¯ãå ç«æž¬å®æ³ã§çšãããã
èçæäœâé µçŽ çµåäœã«ããŠãæäœãšé µçŽ ãšãå
åã®ææãããååŠç¹æ§ãä¿æãããåŠã該çµå
äœãæäŸããããšã§ããã æ¬çºæã®ä»ã®ç®çã¯ãå ç«åŠçç¹æ§ãªããè§Šåª
ç¹æ§ãæå€±ããããšãªãé·æéä¿åããããšã®ã§
ããåŠäžã®çµåäœã«ããŠãèèçã«é¢é£ããã¢ã³
ãã²ã³ãšé«åºŠã«åå¿ããããã®ãæäŸããããšã§
ããã æ¬çºæã®æŽã«å¥ã®ç®çã¯ãåå¿ã«è§Šåªäœçšãå
ãŒããŠæçµçæç©ãé«ãåå¿é床ã§ããããããš
ã®ã§ããé µçŽ ãšçµåããèçæäœãæäŸããããš
ã§ãããèããŠãäžèšæçµçæç©ã®æ¿åºŠã¯ã枬å
忀åºåšãçšããŠæ£ç¢ºã«æ€åºããããã æ¬çºæã®å¥ã®ç®çã¯ãäžè¿°ã®åŠãçµåäœã«ã
ãŠãé«åºŠã«ç²Ÿè£œããèããŠãèèçã«é¢é£ããã¢
ã³ãã²ã³ã®é«æåºŠãé«ç²ŸåºŠå ç«æž¬å®ãä¿é²ããã®
ã«å€§ãã«è²¢ç®ãããšããã®è©²çµåäœãæäŸããã
ãšã§ããã æ¬çºæã®å¥ã®ç®çã¯ãæ¢è¿°ã®è©Šéšã§èšããåŠã
æçšãªäžæº¶æ§ãããªãã¯ã¹ã«ããŠããã®è¡šé¢å šäœ
ã«é«æ¿åºŠã®ç²Ÿè£œèçæäœãäžæ§ã«ååžããããã®
ãæäŸããããšã§ããã æ¬çºæã®ä»ã®ç®çã¯ãèçæäœãåºäœã«çµåã
ããããšã«ãã€ãŠãããã䜿çšäžåãããããã
ãªãæ©æ¢°åãªããã¯ååŠçåã«ãã€ãŠé€å»ãããª
ãããã«ããããšã§ããããã®ç¹ã«é¢ããŠçµ¶å¯Ÿã«
å¿ èŠãªããšã¯ãäžèšçµåãè¡ãéæäœãå®èœæ§ã®
ãŸãã§ããããã«ããããšããªãã¡æäœããã®å
ç«åå¿ã«åäžã§ãèããŠè©²çµåã«ãã€ãŠå ç«ååŠ
çã«å€æ§ãããªãããã«ããããšã§ããã æ¬çºæã®ä»ã®ç®çã¯ãå ç«æž¬å®ã®éæäœã§è¢«èŠ
ãããäžæº¶æ§åºäœãåæ±ããããããã«äžã€ãŸ
ããæž©çœ®ã®éäœ¿çšæº¶æ¶²ã«ãã®è¢«èŠè¡šé¢ã®ããå€ã
éšåãå®éæŽé²ãããããã«è©²äžæº¶æ§åºäœã®åœ¢ç¶
ãæ¹åããããšã§ããã åŠäžã®ç®çããã³ä»ã®ç®çãªãã³ã«æ¬çºæã®ç¹
城ã¯ãäžã€ã®éžå®ãããèããŠå¥œãŸããå ·äœåã
以äžèª¬ç€ºããããšã«ãã€ãŠåœæ¥è ã«ã¯æãããšãª
ããã ãã®åã«ãæ·»ä»å³é¢ãç°¡åã«èª¬æãããªãã 第ïŒå³ã¯ãæ¬çºæã®æ¹æ³ã®å·¥çšãèŠçŽããæµã
å³ã§ããã第ïŒå³ã¯ãæ¬çºæã®æ¹æ³ããã³è©Šéšã»
ããã«çšããããéžå®ãããäžæº¶æ§éšæã®éèŠå³
ã§ããã 第ïŒå³ïŒç¬¬ïŒå³ããã³ç¬¬ïŒå³ã¯ãæ¬çºæã®å ç«
æž¬å®æ³ã®äžã€ã®æž©çœ®æ®µéãå³ç€ºãã 第ïŒå³ã¯ãæ¬çºæã«åŸã€ãŠè©Šéšããã詊éšè©Šæ
ã®éœæ§å ç«æž¬å®ã®éçŸåºããããµã³ãã€ãããæ§
é ã®æŠç¥ç衚瀺ã§ããã 奜ãŸããå ·äœåã®èª¬æ å ããæ¬çºæããã®æãåºãå šæ§çžã«ãããŠè©³
è¿°ãããæ¬çºæã«åŸã€ãèèçã®æ€åºæ¹æ³ã¯ã第
ïŒå³ã«äŸç€ºããåŠãåã€ã®ç°ãªã段éã§å®æœãã
ãã 第ïŒã®æ®µéã¯ãäžæº¶æ§éšæïŒïŒïŒç¬¬ïŒå³åç §ïŒ
äžã«åºå®ãããæäœã第ïŒå³ã«å³ç€ºããåŠã詊éš
è¡æž äžã«ååšããã¢ã³ãã²ã³ãšåå¿ãããããšã
å å«ããããã®åå¿ã¯ã¢ã³ãã²ã³ãåºå®ããèã
ãŠè©²ã¢ã³ãã²ã³ã¯ã第ïŒå³ã«ç€ºãåŠã第ïŒã®å·¥çš
ã§é µçŽ æšèãããæäœãšåå¿ããŠãè©²é µçŽ æšèã
ããæäœããåºå®ããããã«ãªãã第ïŒå³ã«ç€ºã
åŠããé µçŽ ã¯é©åœãªåºè³ªã«ããããã該åºè³ªã¯é µ
çŽ ã®äœçšãåããŠè²ã®å€åããããïŒç¬¬ïŒã®æ®µ
éïŒããã®è²å€åã¯ãèèçã«é¢é£ããã¢ã³ãã²
ã³ã®ååšãç€ºãææšãšãªããæåŸã®ç¬¬ïŒæ®µéã¯ã
è²ã®åºŠåããèªã¿äžã€ãããå¯Ÿç §åºæºã§åŸããã
å€ãšæ¯èŒããããšãå å«ããã äžã«æŠèšãã詊éšã«å¿ èŠãšããããã®å šãŠãå«
ãæ¬çºæã®èçæ€åºã»ããã¯100åã®è©Šéšã«åã
ããäŒå³ããããæãããªåŠãã以äžã«é瀺ãã
詊è¬ã®éçãæ¯äŸçã«å æžããããšã«ããããã
ããã倧ããªåã¯å°ããªèŠæš¡ã®ã»ããã補é ãã
ããšãã§ããã詊è¬ããã³å¯Ÿç §ãå«ããããã¯äŸ
ãã°ç®±ã®äžã«é åãããŠãäœ¿çšæãŸã§ïŒãïŒâã§
ä¿åããããæŽã«ã100åã®è©Šéšãè¡ãã®ã«åå
ãªåæ°ã®ãã€ã¢ã«ãåãä»ããããã ãŸãã第ïŒå³ã«ç€ºãåŠãæ Œå圢ã«äŒŒã衚é¢ãæ
ãããã®å€åŽã«ã¯èçã¢ã³ãã²ã³ãšåå¿ããæäœ
å±€ãçµåãåçµä¹Ÿç¥ãããéåäœåæ¿ã100ã105
ååãä»ããããããããäžæº¶æ§åºäœåæ¿ã®æ£ç¢º
ãªæ§è³ªãšãã®è£œé æ³ã«ã€ããŠã¯åŸã§é瀺ããã 詊éšã»ããã®ç¬¬ïŒã®è©Šè¬ã¯ãèçé¢é£æäœâã¢
ã«ã«ãªæ§ãã¹ãã¢ã¿ãŒãŒçµåäœã®è©Šæãããªãã
ãã®è©Šè¬ã®æ£ç¢ºãªæ§è³ªããã®è£œé æ³ããã³æçšãª
代æ¿è©Šè¬ã«ã€ããŠã¯åŸã§é瀺ããããã®è©Šè¬ã¯ã
ãã®ãŸã䜿çšã«äŸãããæº¶æ¶²ãšããŠæ¯çµŠãããã 詊éšã»ããã®ç¬¬ïŒã®è©Šè¬ã¯ã400mgã®ïœâãã
ãããšãã«ãã¹ããšãŒãé µçŽ åºè³ªã§ããããã®å
åç©ã¯ãç²æ«åœ¢ã§å®å®ã§ãããããããç·©è¡æ¶²ã«
溶解ãããŠïŒmgïŒmlã®æº¶æ¶²ã«ãããšãã¯æ¯èŒçäž
å®å®ãšãªããåŸã€ãŠããããæº¶æ¶²ã¯äœ¿çšçŽåã«èª¿
補ããªããã°ãªããªãã 詊éšã®éã«çšããããä»ã®ç©è³ªã«ãæãéã®éЬ
ã°ãããªã³ã詊æåžéå€ãªãã³ã«çš®ã ã®ç·©è¡æ¶²ã
ãã³æŽæµæ¶²ãå å«ãããããããã®è©Šè¬ããã³ç©
è³ªã®æ£ç¢ºãªæ§è³ªããã³è£œé æ³ã«ã€ããŠã¯åŸã§è¿°ã¹
ãã  æäœã®ç£ç æ¬çºæã宿œããããã«ãèèçã«é¢é£ããã¢
ã³ãã²ã³ãšåå¿ããæäœãååŸããããšãå¿ èŠã§
ãããæ³šæãã¹ãã¯ããããæäœãååšãããšã
ãããšèããŠãæ¬çºæãç¹å®ã®æäœã®äœ¿çšã«éå®
ããã€ããã¯ãªããšããããšã§ããã åå¿æ§æäœã¯ãæ¢ç¥ã®ã¢ã³ãã²ã³ãªããæå詊
æãæ³šå ¥ãããå¯äž»åç©ããæ¡è¡ãããã®ã粟補
ããããšã«ãã€ãŠçšæããããšãã§ãããèèç
ã«é¢é£ããã¢ã³ãã²ã³ãšåå¿ããæäœã¯æŠããè±
åœç¹èš±ç¬¬1387625å·ã«é瀺ãããæ¹æ³ã«ãã€ãŠç£
çããããã èçæäœã®ç£çã¯å ããèçã«éé£ããã¢ã³ã
ã²ã³ã«ã€ããŠéœæ§ã§ããããšãããã€ãŠããè¡æ¶²
ã®ååŸã«äŸæ ãããåŸã€ãŠãçš®ã ã®çµŠæºããåŸã
ããè¡æ¶²ãŠãããã¯å ãããããæ¬çºæã®ç²Ÿè£œã
ããå ç«ç¹ç°æ§æäœãç£çããã®ã«é©ããŠããã
ã©ãããæ±ºå®ãã¹ãè©äŸ¡ãããã°ãªããªãã èçã¢ã³ãã²ã³ã«é¢ãéœæ§ãšæãããè¡æ¶²è¢ã»
ã°ã¡ã³ããïŒãïŒâã§åçŽäœçœ®ã«ä¿ã¡è¡çãäžå
åã«èœã¡çãããã«ããã該è¡çããè¡ãããã
åé¢ããããèããŠããã®åžéåã®è¡ããããšã
ããŠççé£å¡©æ°Žã§ïŒïŒ16ã«åžéãã詊æã®ãåºæº
æäœã«å¯Ÿããå䟡ãåšç¥ã®é黿°æ³³åïŒCEPïŒ
æ³ã«ãã€ãŠè©ŠéšãããåžéããŠãªãè¡ããããš
ïŒïŒ16ã«åžéãã詊æãããããéœæ§ã§ãããšã
ã¯ããã®è¡æ¶²ãŠãããã¯ãåŸè¿°ã®åŠãæ¬çºæã®ç²Ÿ
補æäœã®èª¿è£œçšã«å容ããããšèªããããã è¡æ¶²ãŠãããããåé¢ãããã¢ã³ãã²ã³éšå
ã¯ãå¯äž»åç©äœå ã§ã®æäœç£çãåºæ¿ããã®ã«çš
ããããæã¯è©²åç©ã«ãã€ãŠç£çãããæäœã粟
補ããã®ã«çšãããããã¢ã³ãã²ã³ããããã«çš
ããããã«ããããã®åã«ãããååé¢ããã»ã¹
ã«ä»ããªããã°ãªããªãã è¡ãããã¯ç¡èã®æžå§å®¹åšã«ç§»ãããããã§ã
CaCl2ã®5M溶液ããè¡ããã200mlã«ã€ã
Cacl20.75mlã®åºæºã§å ããããšã«ããååºãã
ããæ¬¡ãã§ããã®æº¶æ¶²ã氎济äž37âã§ïŒæé枩眮
ãåã¯ãåå¡ã圢æãããŸã§æž©çœ®ãããå ãåå¡
ã圢æããåŸãè¡ããããâ20âã§åçµããäžã€
ïŒãïŒâã§èè§£ãããŠåå¡ãåçž®ãããããè¡æž
ãåå¡ããåé¢ããããããå¿ èŠã«å¿ãéãã
ãšããã®ãã®ã¯ãã®ãŸããå ç«åçšèçã¢ã³ãã²
ã³ãã¬ããã®è£œé ã«äŸãããæã¯å ç«åžåå¡ã§ã®
䜿çšã«äŸãããã (1) å ç«åçšã¢ã³ãã²ã³ãã¬ããã®è£œé äžèšããã»ã¹ã«ä»ããèçéœæ§è¡æž ãïŒâã§30
åé10000rpmã®é å¿åé¢ã«ãããããã®é å¿å
é¢ã®äžæŸã¿æ¶²ãè¶ é å¿ç®¡ã«é åããŠãäŸãã°ãã
ã¯ãã³ïŒBeckmanïŒL2â65Bæ©ã§ïŒâãïŒã20æ
é40000ã50000rpmã®è¶ é å¿ã«ä»ãããå管å ã®
äžæŸã¿æ¶²ãåãåºãã廿£ãããã¢ã³ãã²ã³ãå«
ããã¬ããã¯ãèŠå®ïŒ0.15MïŒé£å¡©é¡ã§åããã
ããã æ¬¡ãã§ãåé å¿ç®¡ã«å°å®¹éã®ççé£å¡©æ°Žãå
ãã管å ã®å 容ç©ãé³é¿æ¯åïŒsonicationïŒã«ä»
ããŠãããã厩å£ããããæ¬¡ãã§ãåãœãã±ãŒã¿
ããŠãŒãå ã®æžæ¿ç©ãäžç·ã«ãïŒããŒã«ããïŒã
ããå床ãççé£å¡©æ°Žã®å ¥ã€ãæž æµãªããŠãŒãã«
çé åããããã®æº¶æ¶²ãåã³ãäžèšã®åŠãããã
ã¯ãã³L2â65Bé å¿æ©ã§ïŒâãïŒã20æé40000
ã50000rpmã§é å¿ããã äžæ®µã«èšèŒã®æé ã¯ïŒåãªãããã以äžå埩ã
ãããšãã§ããã åé å¿ç®¡ããäžæŸã¿æ¶²ãåãé€ããåŸããã¬ã
ãç©è³ªãæå°å®¹éã®ççé£å¡©æ°Žäžã«ããŒã«ããã
詊æãåºæºèçæäœã«å¯ŸããŠåæããããããã¬
ãããCEPã«ããïŒïŒ25åã¯ãã以äžã®å䟡ã
瀺ããªãããããå ç«åã«çšããããšãã§ããã
ããŒã«ããã¢ã³ãã²ã³ãã¬ãããïŒmlãã€ã®åå
éã«åããŠå°æ¥ã®äœ¿çšã«åãâ20âã§åçµããã (2) èçæäœã®ç£çããã³å粟補 äžè¿°ã®åŠã調æŽããã¢ã³ãã²ã³ãã¬ããã®è©Šæ
ããå ç«åã®æãç容ã®ããã€ã³ãïŒFreundïŒ
å®å šè£å©æ¶²ã«æ·»å ããŠãåœæ¥è ã«åšç¥ã®æé ã«åŸ
ããšãã«ãžãšã³ã調補ãããæ¬¡ãã§ã該ã¢ã³ãã²
ã³ãå¯äž»åç©äŸãã°éЬã«ãããèªäœæ¢ç¥ã®æè¡ã«
åŸã泚å°ããŠèçæäœãçºçãããå ç«æ§ãä»äž
ãã銬ããæ¡è¡ããæã¯ãã®éŠ¬ãæ £çšæè¡ã«ãã
è¡ãããæ¬åºæ³ïŒplasmapheresisïŒã«ä»ããã
奿³ãšããŠåã¯è¿œå çã«ãä»ã®å ç«ä»äžã«ãŒãã§
è£å©æ¶²äžåšã®èª¿å€ãçšããããšãã§ããã æ¡åããè¡æ¶²ã¯ãåŸã®å ç«åžåâ粟補工çšãäº
æããŠèçæäœãåé¢ãã¹ãåŠçããã°ãªããª
ããæŠæ¬ããã«ããã®å粟補ã¯ïŒæ®µéã§éè¡ãã
ããå ããå¯äž»åç©ããã®è¡ããããåç³ç°åã
ããæ¬¡ãã§ãäžæº¶æ§ã¢ã³ãã²ã³âæäœã®é¯åœ¢æã
æ¹èµ·ããããšã«ããèçé¢é£æäœä»¥å€ã®æäœãæ²
éãããã®ã«ååéã®æ£åžžäººè¡ãããïŒNHPïŒ
ãšäžã§åŸãè¡æž ãšãæ··åãããåžåãããæè¡æž
ããCEPã䜿ã€ãŠèçæäœã«é¢ãåæãããæ
åŸã«ãèçã«é¢é£ããã¢ã³ãã²ã³ãšåå¿ããæäœ
ãç¡«é žã¢ã³ã¢ããŠã ã§æ²æ®¿ãããããã®ç©è³ªã¯äœ¿
çšæãŸã§åçµããããšãã§ããã (3) å ç«åžåå€ãã€ãŒã³ãŒã«å¡ã®è£œé æ¬çºæã®è©Šè¬ãçæããã®ã«çšãããã粟補æ
äœã®è£œé ã¯ãäžè¿°ã®åŠãç£çãããèçæäœãå
ç«ç¹ç°æ§æœåºããã»ã¹ã«ä»ãããšã«ããéæãã
ãããã®æé ã®ç·èª¬ãããã¢ã³ãã²ã³ãšæäœã®å
ç«åŸç°æ§åé¢ãïŒImmunospecific Separation of
Antigens and AutibodiesïŒããšé¡ãããã©ãã€
ãã·ãŠïŒBradishïŒçã®1975å¹ŽïŒæ19æ¥ä»ãè±åœ
ç¹èš±ç¬¬1387625å·ã«èšãããŠããã®ã§å¿ èŠã«å¿ã
ãããåç §ããããã äžè¬ã«ããã®ç²Ÿè£œããã»ã¹ãæ¬çºæã§å©çšãã
ãšããããã¯ãæäœãèçã¢ã³ãã²ã³ãšåå¿ããŠ
é¯åœ¢æãèããŠãæ¡è¡ããæœåºãããæäœè©Šæäž
ã«äžå¯é¿çã«ååšããä»ã®å€æ¥æäœããã³ããçœ
質ãé€å€ãããšããæäœã®èœåãå©çšããã åæŽæµããåžåå€ã«ãŒãã³ãã¬ã©ã¹ç®¡ãªããã
ã©ã¹ããã¯ç®¡ã«æ £çšæè¡ãçšããŠå ãŠãããããš
ã«ãããå¡ãçšæãããèçã¢ã³ãã²ã³ã®ç°ãªã
æ··æç©ãåŸãããã«ãå¥åã®è¡æž 詊æå°ããšãïŒ
åããã¢ã³ãã²ã³ãããŒã«ãããæ¬¡ãã§ãããŒã«
ããã¢ã³ãã²ã³ããæŽå€åžåãåºã«ããŠïŒãïŒ
mgïŒmlïŒæº¶åªïŒã®ããçœè³ªæ¿åºŠã«èª¿æŽããã ã¢ã³ãã²ã³ãã«ãŒãã³ã«ä»çãããã¹ãããã®
åžæº¶æ¶²ã300ã1000mlïŒhrç¯å²ã®æµéã§åžåå¡ã«
å°å ¥ããããã®éãåžåå¡å ã®ãã€ãŒã³ãŒã«ïŒïœ
åœã75mgã®ããçœè³ªãæ·»å ããããã«ãã¹ãã§ã
ããåžåå¡ããã®æµåºç©ã500mlã®ååéã§é
ãããã®åã ãããçœè³ªå«éã«ã€ããŠæ€æ»ããã
æµåºç©ãåºçºç©è³ªã®ããçœè³ªå«éã«ã»ãçãã該
å«éã瀺ããšããåžåå¡ã¯ã¢ã³ãã²ã³ã§é£œåãã
ããšèªããããããã€ãŒã³ãŒã«å±€ã¯ãæµåºç©ã
280nmã«ãããŠç®ç«ã€çšã®æ€åºå¯èœãªåžåã瀺ã
ãªããªããŸã§å¡ã«ç¡«é žå¡©ç·©è¡å€å ¥ãé£å¡©æ°Ž
ïŒPBSïŒãæµãããšã«ãã€ãŠããã€ãŒã³ãŒã«å±€ã
æŽæµããã ãããçµåããããçœè³ªã溶é¢ãã¹ãããã€ãŒ
ã³ãŒã«å±€ãæ°ãã調補ããããªç¡«é žãããªãŠã
200mgïŒå ¥ã5MããåãããªãŠã æ¶²ã§ãã©ãã·
ãŠïŒæŽãæµãïŒããããã®åŸãåžåå¡ã«ååéã®
PBSãæµãå ¥ããŠã該å¡ããäžèšã®ããåãããª
ãŠã æ¶²ãæµå»ãããèããŠãïŒmgïŒmlã®ãããªãŠ
ã ã¢ãžãïŒé²è å€ïŒã嫿ããPBCã§åžåå±€ãæ
çµçã«æŽæµããåŸãå¡ãïŒãïŒâã«ä¿åããããš
ã«ãããåŸã®äœ¿çšã«åããããšãã§ããã (4) é µçŽ çµåäœã«çšããããæäœããã³äžæº¶æ§åº
äœã®è£œé äžè¿°ã®åŠã粟補ããæäœã¯ç¡«é žã¢ã³ã¢ããŠã ã
ãªãããããPBSã«ãã€ãŠãæäœïŒéšå¯Ÿç·©è¡å€ïŒ
éšã®åºæºã§åžéãããå¡ãæŽåããç»åãéãã
ããããã«äœçœ®ã¥ããããã®å¡ã«ãäžèšã®æäœæº¶
æ¶²ãçŽ200mlïŒhrã®æµéã§é£ç¶çã«æ·»å ãããæµ
åºç©ãéãããããããçœè³ªããã³èçæäœå«é
ã«ã€ããŠè©Šéšããããšã«ãããå¡ããã€æäœã§é£œ
åãããããæ±ºå®ããã飜åãéããããåŸãå¡
ã®å±€ãPBSã§æŽæµããŠãããåžåãããããçœè³ª
ãé€å»ããã ãã®æç¹ã§ããã€ãŒã³ãŒã«å±€ã«åºå®ããã¢ã³ã
ã²ã³ã¯ããã®ãã®ãšåå¿ããæäœãšçµåããŠã
ãããŸããå¡ãéã€ãŠããä»ã®å€æ¥ããçœè³ªãã
ã³æäœã¯ããã®å±€ã«åºå®ããã¢ã³ãã²ã³ã«å¯ŸããŠ
éç¹ç°æ§ã§ãããèããŠäžèšæäœããåé¢ããŠã
ãã ãã®æäœâã¢ã³ãã²ã³çµåãç Žæãäžã€ç²Ÿè£œæ
äœã溶é¢ãã¹ãã䜿çšçŽåã«èª¿è£œããNaIã®5M溶
æ¶²ãå¡ã«å°å ¥ããããã®NaI溶液ã®äœ¿çšå®¹éã¯ã
å±€ã«çµåããæäœãå šãŠåãåºãã®ã«ååã§ãã
ã¹ãã§ããã塿µéãå°ããšã20mlïŒhrã«èšå®ã
ããšããæº¶åºæ¶²ãé©åœãªå®¹éã®ç»åã§éããã
ããéãŸã€ãåèšéã¯å°ããšããããåãããªãŠ
ã ïŒNAIïŒæº¶æ¶²ã®æ·»å 容éã«çããããã¹ãã§ã
ãã 粟補æäœã®åç»åã¯ãéãããããšäºåã®é
ã«ä»ããããããªãã¡ãäžåç®ã¯0.45ÎŒã®èã«é
ããäºåç®ã¯0.22ÎŒã®èã«éããæ¬¡ãã§ãïŒãïŒ
âã®èžçæ°ŽãçšããŠãåŸãããæ¶²ãïŒïŒïŒã«åž
éãããäŸãã°200mlã®æ¶²ã40mlã®èžçæ°Žã«æ·»
å ãããæ¬¡ãã§ãåžéãããããæäœç»åããäŸ
ãã°ãXMâ50ã®èãåããã¢ãã³ã³
ïŒAMICONïŒæ¿çž®æ©ã«å ¥ããŠæ¿çž®ããã æçµç²Ÿè£œå·¥çšãšããŠããã®æ¿çž®ããã粟補æäœ
ãéæãããéæã®åŸãæäœãåãåºããŠé å¿å
é¢ã«ããããäžæŸã¿æ¶²ãå°ããšã20æé0.01Mã®
ç¡«é žãããªãŠã 溶液ã«å¯ŸããŠéæãããããPBS
ã«å¯Ÿããåãã®éæãšæ¯èŒããããã®åŸã®éæã
çµäºããåŸãæäœã®ããçœè³ªæ¿åºŠã枬å®ããã æ¬¡ãã§ããã®æäœã«ã€ããŠãCEPæè¡ã«åŸã
åºæºã¢ã³ãã²ã³ã«å¯ŸããæŽ»æ§åºŠã枬å®ããŠæäœå«
éãæ±ºå®ãããŸãããåãå ¥ãããããšããã€ã
ãããããåçµä¹Ÿç¥ããŠäœ¿çšæãŸã§ä¿åããã  æäœâé µçŽ çµåäœã®è£œé åçã®è žå ã¢ã«ã«ãªæ§ãã¹ãã¢ã¿ãŒãŒãåæ§æ
ããæäœã®æº¶æ¶²ã«ãïŒïŒïŒã®é µçŽ ïŒæäœæ¯ã§æ··å
ããããšã«ãããæçµæ¿åºŠãBPSïŒPH7.4ïŒæº¶æ¶²
ïŒmlã«ã€ãå šããçœè³ª10mg以äžã«ãªãããã«ã
ãããã®æº¶æ¶²ãéæããŠNH4 +ã€ãªã³ãå®å šã«é€
å»ããã éæåŠçãããæäœâé µçŽ æ··åç©ã次ãã§é å¿
åé¢ã«ããäžæº¶æ§ç©è³ªãå šãŠé€ããPBSâMgã
æº¶æ¶²ã®æ·»å ã«ãããäžæŸã¿æ¶²ã®ããçœè³ªå«éã10
mgïŒmlã«èª¿æŽããããã®æº¶æ¶²ã«ïŒïŒ ã®ã°ã«ã¿ã«ã¢
ã«ãããæº¶æ¶²ããæäœâé µçŽ æº¶æ¶²10mlã«ã€ãã°ã«
ã¿ã«ã¢ã«ãããæº¶æ¶²ïŒmlã®åºæºã§æ·»å ããã3.5
ã20åéç·©åŸã«æ¹æãèããŠãã®éãæäœãšé µçŽ
ãã°ã«ã¿ã«ã¢ã«ãããã«ãã€ãŠååŠçµåããåŸã
ãã®çµåäœã®æº¶æ¶²ã0.001Mã®Mgãå ¥ãPBCã«å¯Ÿ
ããŠéæããŠã°ã«ã¿ã«ã¢ã«ããããé€å»ããã æ¬¡ãã§ãéæåŠçããç©è³ªãé å¿åé¢ã«ããã
åŸãããäžæŸã¿æ¶²ã«ããªã¹ïŒããããã·ã¡ãã«ïŒ
ã¢ããã¡ã¿ã³ïŒPHïŒïŒç·©è¡å€0.05Mãæ£åžžäººã¢ã«
ããã³ïŒçµæ¶è³ªïŒïŒïŒ ãNaN30.02ïŒ ããã³
MgCl20.001Mã®æ°Žæº¶æ¶²ãæ·»å ããŠãããåžéã
ãã ã¢ã«ã«ãªæ§ãã¹ãã¢ã¿ãŒãŒã«å ããŠãä»ã®é µçŽ
ã亊æ¬çºæã®æ¹æ³ã«çšããããšãã§ãããäºå®ã
æäœã«å ±æçµåãããé µçŽ ãšããŠã»ãšãã©ç¡éã®
ãã®ãæããããããããçš®ã ã®é µçŽ ã®ãã¡ä»¥äž
ã«åæãããã®ãç¹ã«æå©ã§ããã ïŒ ã¢ã«ã³ãŒã«è±æ°ŽçŽ é µçŽ ïŒ ã°ãªã»ããŒã«è±æ°ŽçŽ é µçŽ ïŒ ã°ãªãªãã·ã¬ãŒãéå é µçŽ ïŒ ïŒ¬âä¹³é žè±æ°ŽçŽ é µçŽ ïŒ ãããé žè±æ°ŽçŽ é µçŽ ïŒ ã°ã«ã³ãŒã¹ïŒâããé žè±æ°ŽçŽ é µçŽ ïŒ ãã³ãããïŒâããé žè±æ°ŽçŽ é µçŽ ïŒ ã°ã«ã³ãŒã«é žåé µçŽ ïŒ ã¬ã©ã¯ããŒã¹é žåé µçŽ 10 âã¢ããé žé žåé µçŽ 11 âã¢ããé žé žåé µçŽ 12 ããªããšããŒã«é žåé µçŽ 13 ã¢ã¹ã³ã«ãã³é žé žåé µçŽ 14 ã«ã¿ã©ãŒãŒ 15 ãã«ãªãã·ããŒãŒ 16 ã³ãªã³ãšã¹ãã©ãŒãŒ 17 ãã¹ããªãªããŒãŒïŒ£ 18 αâã¢ãã©ãŒãŒ 19 ãªãŸããŒã 20 βâã¬ã©ã¯ãã·ããŒãŒ 21 ã¢ããã°ã«ã·ããŒãŒ 22 βâã°ã«ã¯ããããŒãŒ 23 ã«ã«ããã·ãããããŒãŒïŒ¡ 24 ãŠã¬ã¢ãŒãŒ 25 ç¡æ©ãããã¹ãã¢ã¿ãŒãŒ 26 ã¢ã«ãã©ãŒãŒ 27 çé žè±æ°Žé µçŽ 28 ãã¹ãããŒãŒ æäœãæšèããã®ã«çšããããé µçŽ ã¯ãããã€
ãã®èæ ®ãã¹ãäºæã«çæããŠéžå®ãããããã
ãçæãã¹ãäºæã«ã¯ãé µçŽ ã®å®å®æ§é µçŽ ã®æž¬å®
å®¹ææ§ãæäœã®å ±æçµåã®æ¡ä»¶ã«å¯Ÿããé µçŽ ã®è
ä¹ æ§ãªãã³ã«é µçŽ ã®å ¥ææ§ããã³äŸ¡æ Œãå å«ãã
ãã  æäœã§è¢«èŠãããäžæº¶æ§éšæã®è£œé 詊è¬è£œé ã®æ¬¡ã®å·¥çšã¯ã粟補æäœã®äžéšåãäž
溶æ§éšæã«å ±æçµåãããããšã§ããããã®çµå
ãè¡ãããã«ãçšããããäžæº¶æ§éšæã¯ãåå¿åº
åã¯çç©è©Šéšã«çšããããç¹ç°æ§æäœãšåå¿ãã
ãéšäœãäžããããŠããªããã°ãªããªãã ãã°ã©ããã»ã³ããªããŒãºïŒGraft
CopolymersïŒããšé¡ããG.W.ãã¬ã®ã¢ãŒ
ïŒTregearïŒçã®ç±³åœç¹èš±ç¬¬3700609å·ã«ã¯ãæã
éåäœäž»éã«å¥ã®éåäœåã¯å ±éåäœã®åŽéãã°
ã©ãããããŠãªãäžæº¶æ§é£ç¶éåäœç©è³ªãé瀺ã
ããŠããããã®ã°ã©ãããããéåäœãé©å®éžå®
ããããšã«ãã€ãŠãçç©ç©è³ªãäžæº¶æ§ãããªãã¯
ã¹ã«ååŠçµåãããããšãã§ãããäžèšç¹èš±ã«é
瀺ãããçæç©ã¯ãã€ã³ããªã¢ã«ã»ã±ãã«ã«ã»ã€
ã³ãã¹ããªãŒãºã»ãªãã»ãªãŠã¹ãã¬ã€ãªã¢ã»ã¢ã³
ãã»ããŠãŒãžãŒã©ã³ãïŒICIANZïŒããåååã
ãã¿ããŒã«ïŒPROTAPOLïŒDIïŒïŒã®åæ¿åœ¢ã§åž
販ãããŠããã ããã¿ããŒã«DIïŒïŒã¯ãã€ãœããªã·ã¢ãããª
ã¹ãã¬ã³åºã衚é¢å šäœã«ã°ã©ãããããããªãã
ã©ãã«ãªããšãã¬ã³äž»éãããªããæŸå°å ç«æž¬å®
æ³ã«äœ¿çšãã¹ãäŒå³ãããŠãããçŸåšå ¥æããã
ããã®çš®ã®åæ¿ã¯ãåããçŽ0.01ã€ã³ããçŽåŸã
çŽ0.5ã€ã³ãã§ããã 第ïŒå³ã«ç€ºãåŠãæ¬çºæã®äžã€ã®éèŠãªå ·äœå
ã«åŸãã°ãååæ¿ïŒïŒã¯ãäžé£ã®çšç·ïŒïŒãšå¥ã®
äžé£ã®çšç·ïŒïŒãšã§æ§æãããæ Œå圢çŒã網暡æ§
ã®äž¡é¢ïŒïŒãä»äžãããŠãããçšç·ïŒïŒããã³ïŒ
ïŒã¯å¥œãŸããã¯äºãã«åçŽã§ããèããŠãããã¯
è€æ°åã®ç©åœ¢ããŒã¿ïŒïŒãç»æãããåçšç·ïŒïŒ
ããã³ïŒïŒã®åŽé¢ã¯ãããŒã¿ïŒïŒã®é£æ¥å¯Ÿããäž
æ¹åãžãšæ¬¡ç¬¬ã«ããŒããŒïŒæé¢åãæžå°ïŒããŠã
çšç·ã®é éšãç»æããç·ãšãªããæ³šæãã¹ãããš
ã¯ãå³é¢ã®çšç·ïŒïŒããã³ïŒïŒããæ¬çºæã®èª¬æ
ãç°¡ç¥åããããã«éåžžã«èªåŒµããŠæãããŠãã
ãšããããšã§ããã åæ¿ã®ææåœ¢ç¶ã¯ã該ææåœ¢ç¶ãåæ¿ã«ä»äžã
ã¹ãäŒå³ããããçªèµ·ã衚é¢ã«ãã€ããŒã©ãŒã«è©²
åæ¿ãéãããšã«ãã€ãŠéæããããæãããªã
ãã«ããã®ããŒã©ãŒã¯ãåæ¿ãäºå®äžç Žå£ããã
ãšãªããã®éåäœç©è³ªã®å€èгãå€åœ¢ããã«ååé
ã®å§åãããããããã«èšèšãããŠããããã
ã¯ãåæ¿ããã®è¡šé¢ã«åå¿æ§å±€ãæããŠããã®ã§
éèŠã§ãããããããŠãåæ¿ãå°ã¡æãããšã«ã
ããæäœãçµåããããšã®ã§ããªãåéšãæŽé²ã
ãããããªããã©ãã«ãªããšãã¬ã³å±€ã®æŽé²ã¯äº
å®äžãçµåèœåã®äœãåæ¿ãããããã§ãããã èæ ®ãã¹ãäž»ãªäºæã¯ãå¹³åºã®ãã€ã¢ã«ã«å ¥ã
ããšãå®è³ªäžè©Šéšè©Šæãšå®å šæ¥è§Šã®ç¶æ ã«ãªãã
ããªäž¡é¢ããã€åæ¿ãããããããšã§ãããããª
ãã¡ããããªãã¯ã¹ãšãã€ã¢ã«åºãšã®éã®é¢å士
ã®æ¥è§Šãæå°éã«ãã¹ãã§ãããæ¬çºæã®å¥ã®é
èŠãªå ·äœåã§ã¯ãåæ¿ã®äž¡é¢ã¯ãé«äœç¹åéãã
ã€ããã«åœ¢ã¥ããããŠããã æ¢è¿°ã®åŠããåçµä¹Ÿç¥åœ¢ã«è£œé ãããæäœã¯ã
æäœ5.0mgæ¯ã«0.1Mã®NaHCO3ïŒPH9.6ïŒã100ml
æ·»å ããããšã«ãã€ãŠåæ§æããããäžè¬ã«ãã
ã®çµåæ¹æ³ã¯ãïŒãïŒâã®è©²åžæº¶æ¶²ã«æ¹æäžãæ Œ
å圢ãªããçŒã網暡æ§ã®è¡šé¢ããã€åæ¿ãïŒã16
æéæ¥è§Šãããããšãå å«ãããæ¬¡ãã§ãæäœåž
溶液ã廿£ããåŸãåæ¿ããäºåºŠé£ç¶å®¹éã®
0.1M NaHCO3ïŒPH9.6ïŒããé žå¡©ç·©è¡å€å ¥ãé£å¡©
æ°Žããã³ã0.5ïŒ ã®ããŠã€ãŒã³ïŒTWEENïŒ20ã
嫿ããããé žå¡©ç·©è¡å€å ¥ãé£å¡©æ°Žäž0.3ïŒ ã®å·
ãïŒïŒãïŒâïŒçè¡æž ã¢ã«ããã³ã§æŽæµãããæŽ
ã«ãçµæ¶è³ªã®çè¡æž ã¢ã«ããã³ã§æŽæµãäžã€ãã©
ã€ã¢ã€ã¹äžã§åçµãããåŸãåçµä¹Ÿç¥ãè¡ããè
ããŠãã®åæ¿ã䜿çšã«äŸããŸã§ïŒãïŒâã§ä¿åã
ãã æ¬æçްæžäžã®èšèŒã¯ãèçæäœãçµåãããå
æ¿ã®è£œé ã«ä¿ãããã©ããæ¬çºæã®åæ¿ãã»ãšã
ã©ç¡éã®æ°ã®ããçœè³ªãåºå®ããã®ã«æçšã§ãã
ããšã¯åœæ¥è ã«æããã§ããããäŸãã°ã詊éšè©Š
æâåæ¿éã®æ¥è§Šå¢å ã«ãã€ãŠãäžèšããçœå®€ã
è©²åæ¿ãšã®çµåãå å«ãã詊éšã«çšããããšãã§
ãããããªãã¡ããããããçœè³ªã«ããžã³ãã·
ã³ãã¢ãã³å€ããã³ã¹ããã€ãã®åŠãè¬åã«å¯Ÿã
ãæäœïŒå€©ç¶ç£ç©äŸãã°ã€ã³ã·ãŠãªã³ããã³ä»ã®
ãã«ã¢ã³ã«å¯ŸããæäœïŒãªãã³ã«ãè¡æ¶²ãã®ä»ã®
äœæ¶²äžã«èŠåºããã代è¬ç£ç©ã«å¯Ÿããç¹ç°æ§é µçŽ
ãå å«ãããã äŸ ä»¥äžã®æé ãçšããŠåæ¿8000æã補é ãããå
åæ¿ãå ããæ¢è¿°ã®åŠãææåœ¢ç¶ã«ããããã«ã
ã¬ã¹åŠçãããèçæäœã¯åæ¿8000æã®ãããã«
40mgå¿ èŠã§ãã€ããããªãã¡ãåæ¿1000æã«ã€ã
ïŒmgã§ãããåæ§æããæäœã®ããçœè³ªå«éãã
0.1Mã®NaHCO3ïŒPH9.6ïŒ800mlã®æçµå®¹éã«ãã
ãŠ0.05mgïŒmlã«èª¿æŽãããæ¬¡ãã§ããã®å š800ml
ã®ç·©è¡å€å ¥ãæäœããæŽ©ãæ¢ãã©ã€ããŒãåãäž
ã«ã¯åæ¿8000æã®å ¥ã€ã1000mlã®ããèã³ãã«è£
å ¥ããããããŠããã®ã³ãã16æéäŸãã°äžå€ïŒ
ãïŒâã§å転ãããŠåå転ãµã€ã¯ã«ãéãåæ¿ã
ç·©åŸã«ã¿ã³ããªã³ã°ããããã«ããããã®åŸãæ¶²
ãã³ãããæµãåºã廿£ãããåæ¿ã¯ïŒã®åºå£
ãã©ã¹ã³ã«ç§»ããã åæ¿ãå·ãïŒïŒãïŒâïŒ0.1Mã®NAHCO3ç·©è¡
æ¶²ïŒPH9.6ïŒïŒã§äºåºŠç¶ããŠæŽæµãããã®åŸè©²
ç·©è¡æ¶²ãé€å»ãããæ¬¡ãã§ãåæ¿ãååºŠæŽæµã
ãããã®ãšãã¯ãå·ãç·©è¡å€ïŒ0.01Mã®ããé žã
ããªãŠã ã0.15Mã®NAClãPH7.4ïŒïŒãã€äºåºŠ
ã®é£ç¶æŽæµã§ãã€ããæ®çããç·©è¡å€ãé€å»ãã
åŸãåæ¿ãäžåºŠç®ã®æŽæµã«ä»ãããããã«ã¯ãå·
ããçè¡æž ã¢ã«ããã³æº¶æ¶²ïŒ0.3ïŒ ïŒïŒéãã€
äºåºŠã®é£ç¶æŽæµãçšããã æåŸã«ãåæ¿ããïŒmgïŒmlæ¿åºŠã®å·ãçµæ¶è³ªç
è¡æž ã¢ã«ããã³ïŒPHïŒïŒæº¶æ¶²ïŒéãã€äºåºŠã®é£
ç¶æŽæµã«ä»ããããã®å·¥çšã¯ãåæ¿äžã®ããçœè³ª
ã«ããçœè³ªç°å¢ãäžããç®çã§å®æœããããæ®ç
æŽæ¶²ãé€å»ããåŸãåæ¿ããäžæã®çŽã§å 匵ã
ããç¿ãªããã¯ãã¬ãŒïŒïŒÃ9inïŒã«ç§»ããã該
ãã¬ãŒã«ã¯å€«ã ã200mlã®çµæ¶è³ªçè¡æž ã¢ã«ãã
ã³æº¶æ¶²ãå ¥ããŠãã€ããç§»ãçµã€ãåŸãäžæã®
çŽã䜿ã€ãŠããããèŠã€ããç·©è¡å€ãå®å šã«é€å»
ããæ¬¡ãã§åæ¿ããã©ã€ã¢ã€ã¹äžã§30忥éã«å
çµãããã ãããåŸããã¬ãŒã®å 容ç©ãåçµä¹Ÿç¥ããã也
ç¥ããåæ¿ãåãåºãããããç®æ¢ããã容åšå
ã§è²¯èµããã (1) å¯Ÿç §è¡æž ã®èª¿è£œ å ç«æž¬å®ããæå³æ·±ãè³æãåŸãããã«ãé°æ§
ãšéœæ§ã®å¯Ÿç §è¡æž ãçšæããŠæå®ã®è©Šéšè©Šæãšé©
宿¯èŒããå¿ èŠãããããããå¯Ÿç §ç©ã®èª¿è£œã以
äžã«è©³è¿°ããã é°æ§ã®å¯Ÿç §ã¯ãäŸãã°æŸå°å ç«æž¬å®æ³ã«ããã
èçã¢ã³ãã²ã³ã«ã€ããŠè©Šéšãé°æ§ãšããã€ã人
è¡ããããã補é ãããèããŠãèçã¢ã³ãã²ã³
ã«é¢ãæããã«é°æ§ã§ããåãŠãããã«ã5Mã®
CaCl2ãè¡ããã200mlã«ã€ãCaCl2溶液0.75mlã®
åºæºã§æ·»å ããŠåå¡ãèªåŒãããæ¬¡ãã§ãåå¡ã
圢æãããŸã§ãã®è¡ããããæ°ŽæµŽäž37âã§æž©çœ®ã
ããåå¡ããè¡ããããŠããããæ¬¡ãã§â20âã§
åçµããŠå°ããšã12æé貯èµãããæ¬¡ãã§ããã®
è¡ããããŠããããïŒãïŒâã§èè§£ãããè¡æž ã
éãããããè¡æž ãéåºŠã«æ¿ã€ãŠãããããã
ããäŸãã°ïŒãïŒâã§30åé9000rpmã®é å¿åé¢
ã«ããæž æŸåããããšãæãŸãããããããªãã ã·ãªã«äŸãã°ãšã¢ããžã«ïŒAEROSILïŒâ380
ãè¡æž ïŒåœã20ïœå ããïŒæéå®€æž©ã§æ··åããŠ
ãªããããã€ã³ãé€å»ãè¡æž ãå®å®åããããæ¬¡
ãã§ããã®æ··åç©ãé å¿åé¢ã«ãããæ²æ®¿ç©ãå»
æ£ãããããææãªããé©åœãªéææ®µã«ãã€ãŠ
éããŠã·ãªã«ãé€å»ããããšãã§ããã æ¬¡ãã§ãäžæŸã¿æ¶²ãæŽã«ãäŸãã°ããªãã¢
ïŒMILLIPOREïŒèãªããããŒã ïŒHORMïŒèã
ãã«å€åçãé£ç¶çã«äœäžãããããã§éåŠç
ããããããã¯0.45ãã¯ãã³ã®èã§ããããã®
0.45ãã¯ãã³ã®èã§éããåã«ããããªãŠã ã¢
ãžãïŒNaN3ïŒã0.1ééïŒ ã®æ¿åºŠã§äžèšäžæŸã¿æ¶²
ã«æ·»å ãããæ¯çã§åšç¥ã®åŠãããããªãŠã ã¢ãž
ãã¯é²è å€ãšããŠäœçšããã0.45ãã¯ãã³ãªãã
ãã以äžã®å€åçãã€ã«ã¿ãŒã«ããæåŸã®é
ã¯ãç¡èã®è£ 眮ããã³æè¡ãçšããå±€æµç°å¢ã§è¡
ãã¹ãã§ããã æ¬¡ãã§ããã®ç¡è溶液ã¯ãå ç«æž¬å®çšè©Šè¬ç®æ¢
ãæº¶åšã«å°åãããããŸã§ïŒãïŒâã§è²¯èµããã
ããæç€ºãã100åã®è©Šéšã»ããã«ã¯ã7.5mlã®é°
æ§å¯Ÿç §è¡æž ãäŸäžãããã éœæ§å¯Ÿç §è¡æž ã¯ãèçã¢ã³ãã²ã³è©Šéšã§éœæ§ãš
åºãè¡æ¶²ãŠãããããã®è¡ããããåç³ç°åãã
ããšã«ãã補é ãããåéœæ§ãŠãããããïŒïŒ 詊
æãæ¡åããããããäžç·ã«ããŒã«ããŠè©ŠéšããŒ
ã«ãšãããå ãããã®è©ŠéšããŒã«ã60âã§10æé
ç±åŠçããŠè©Šæäžã®èçèªçºç©è³ªãå šãŠäžæŽ»æ§ã«
ããã該ããŒã«ã宀枩ã«å·åŽãããšããã¢ã³ãã²
ã³æŽ»æ§ãä¿çãããŠããããšãæ€æ»ãã¹ãäžéšå
ãåãåºããCEPæè¡ã䜿ã€ãŠåºæºæäœã«å¯Ÿã
滎å®ãããæ¬¡ãã§ã詊éšããŒã«ã«ãè¡æž ïŒåœã
20ïœæ¿åºŠã®ã·ãªã«ãæ·»å ããããã®è¡æž ãæ¬¡ãã§
ïŒæéå®€æž©ã§æ¹æãããã®åŸãããïŒãïŒâã§30
åé9000rpmã®é å¿åé¢ã«ããããæ²æ®¿ç©ã¯å»æ£
ããã åŸãããäžæŸã¿æ¶²ããCEPã䜿ã€ãŠå¯Ÿç §åºæº
æäœã«å¯Ÿã滎å®ãããããå䟡ãç³åã®ãªãã¬ã
ã«ã®ãŸãã§ããã°ãå šè¡æž ãŠãããã®ç·éãäžç·
ã«ããŒã«ãäžã€ãããã詊éšããŒã«ã§è¿°ã¹ããšå
ãããã»ã¹ã«ä»ãããšãã§ããã äžç·ã«ãã詊éšããŒã«ãšäž»èŠããŒã«ã¯ãæ¬çºæ
ã®å ç«æž¬å®ã§éœæ§å¯Ÿç §è¡æž ã«é¢ãæé©çµæãåŸã
ããããã«ååéã®é°æ§å¯Ÿç §è¡æž ã§åžéããã奜
ãŸããã¯ãæ¬çºæè©Šéšã§ã®éœæ§å¯Ÿç §è¡æž ã®èªã¿ã
åžå 床åäœÃ1000ã§2000ããé«ãããã¹ãã§ã
ãããã®åžéããéœæ§å¯Ÿç §è¡æž ãæ¬¡ãã§ãæ¢è¿°ã®
åŠããé£ç¶ç¯å²ã®äœäžå€åçãçšããé©åœãªææ®µ
ã§éããã0.45ãã¯ãã³åã¯ãã以äžã®èã§æ
çµçã«éããåã«ã0.1ééïŒ ã®ãããªãŠã ã¢
ãžããæ·»å ãããé°æ§ããŒã«ã®ãšãã®ããã«ãã
ã®æåŸã®éã¯ãå±€æµç°å¢äžç¡èæ¡ä»¶äžã§è¡ãã¹
ãã§ããã åŒ±éœæ§å¯Ÿç §è¡æž ã¯ãåŒ·éœæ§å¯Ÿç §ãé°æ§è¡æž ã§åž
éããããšã«ãã補é ããããšãã§ãããåŒ±éœæ§
è¡æž ã®èªã¿ã¯ãæ¬çºæã®è©Šéšã§æž¬å®ãããšã600
ã1000ã§ããã¹ãã§ãããæ¬çºæã®å¥œãŸããå ·äœ
åã®100å詊éšã»ããã«ã¯åŒ·éœæ§å¯Ÿç §2.5mlãšåŒ±éœ
æ§å¯Ÿç §2.5mlãåãä»ããããã  ãã®ä»ã®è©Šè¬ããã³è£ 眮 0.5ã€ã³ããããããã«å€§ããªããªãã¡ãçŽåŸ
0.50ã€ã³ãã®åæ¿ã«ã€ãåã寞æ³ã®çŽåŸãæãã
200ãªãããã以äžã®ã¬ã©ã¹è£œå¹³åºãã€ã¢ã«ïŒäœ¿
ãæšãŠïŒãåãã€ãããããããããã€ã¢ã«ã®ã
ã¡100åã¯ã詊éšã®åãã®æž©çœ®ããã³æŽæµçšã«äœ¿
ãããæ®ã100åã¯ãåŸã®ãé µçŽ åºè³ªãšã®æž©çœ®ã«
çšãããããåŸã€ãŠããã®è©Šéšã»ããã«ããã
100åã®æž¬å®ãåæã«è¡ãããšãã§ããã 以äžã«è©³è¿°ããåŠããäžæº¶æ§åºäœåæ¿äžæãã
ãã100åã®ãã€ã¢ã«åã ã«å ¥ããåã«ã銬ã°ã
ããªã³è©Šéšè©Šæåžéå€ã0.05mlãã€åãã€ã¢ã«ã«
å ¥ããããã®å·¥çšã¯ã詊éšè©Šæãšäžæº¶æ§åæ¿ãšã®
åãã®æž©çœ®ã§éç¹ç°æ§åå¿ãæé€ããäºåææ®µãš
ãšãããåæ¿äžã«è¢«èŠãããæäœã¯ç²Ÿè£œãããŠã
ããèçã¢ã³ãã²ã³ãšé«åºŠã«åå¿ããããã©ãã
äººè¡æž åã¯è¡ãããäžã«ã¯ã銬ã°ãããªã³ãã®ã
ã®ãšåå¿ãåŸèããŠåæ¿ãšé µçŽ æšèçµåäœãšã®é
ã«æ©ã圢æããŠæ¬äŒŒéœæ§åå¿ãããããããç©è³ª
ãææååšãããå ã®å·¥çšã§æ·»å ãã銬ã°ãããª
ã³ã¯ãã®ç©è³ªãšçµåãããã®ããåæ¿ãšã®åå¿ã¯
èªç±ã§ãªããå ã«é瀺ããåŠããåæ¿äžã«è¢«èŠã
ããæäœã¯ãäººè¡æ¶²ããéããèçé¢é£ã¢ã³ãã²
ã³ã§éЬãå ç«åããããšã«ããç£çããããæ¬çº
æã®100å詊éšã»ããã«ã¯ãããé žå¡©ç·©è¡å€å ¥ã
é£å¡©æ°ŽïŒPBSïŒã«éЬã°ãããªã³330mgãæº¶è§£ããŠ
ãªã溶液5.5mlãåãã€ããããã  åºè³ªã®è£œé 詊éšã®é µçŽ åå¿çšã«éžãã åºè³ªã¯ïœâãããã
ãšãã«ãã¹ããšãŒãã§ããããã®ãã®ã¯ãçé žã
ããªãŠã ç·©è¡å€ã«ïŒmgïŒmlã®æ¿åºŠã§æº¶è§£ãããã
ç·©è¡å€ã®æ¿åºŠã¯0.028ã¢ã«ã®çé žãããªãŠã ãã
ã³0.001ã¢ã«ã®ãã°ãã·ãŠã ïŒPH9.8ïŒã§ãã€ãã ãã®æç¹ã§æ³šæãã¹ãããšã¯ãäžã®è¡šã«ç€ºã
åŠãä»ã®åºè³ªãé©åœãªPHç·©è¡å€ãšå ±ã«çšããããš
ãã§ãããšããããšã§ããã
ã衚ã
ããšãŒã
ã衚ã
ãªã«ãã¹ããšãŒã
äžè¡šã«ç€ºããåºè³ªã¯å šãŠææ©ããé žãšã¹ãã«ã§
ããã奜ãŸããé µçŽ ã¢ã«ã«ãªæ§ãã¹ãã¢ã¿ãŒãŒã«
åã€ãåºè³ªãšããŠä»ã®ææ©ããé žãšã¹ãã«ã䜿çš
ããããããšã¯æããã§ãããæŽã«ã泚æãã¹ã
ããšã¯ãã¢ã«ã«ãªæ§ãã¹ãã¢ã¿ãŒãŒä»¥å€ã®é µçŽ ã
æäœâé µçŽ çµåäœã«çšãããšãããé©åœãªåºè³ªã
éžå®ããããšã¯åœæ¥è ã«ãšã€ãŠã»ãšãã©å°é£ã§ãª
ããšããããšã§ããã  æ¹ æ³ è©Šéšãæ¬çºæã«åŸã€ãŠè¡ãã¹ãããã€ã¢ã«100
åãæ¶ã§åºåãããçš®ã ã®è©Šæã«å¯Ÿå¿ããããã«
ããããèå¥ãããåãã€ã¢ã«ã«éЬã°ãããªã³æº¶
æ¶²0.05mlãå ¥ããæ¬¡ãã§95åã®ãã€ã¢ã«ã«è©Šéšè©Š
æ0.5mlãæ·»å ãããåæã«ãä»ã®ãã€ã¢ã«ïŒå
ã«å€«ã ãé°æ§å¯Ÿç §è¡æž ã®è©Šæ0.5mlãæ·»å ããä»
ã®ïŒåã«åŒ·éœæ§å¯Ÿç §è¡æž ã®è©Šæ0.5mlãå ¥ããã
ããŠæ®ãïŒåã«ã¯åŒ±éœæ§å¯Ÿç §è¡æž ã®è©Šæ0.5mlã
å ¥ãããããã銬ã°ãããªã³ãšå詊æãå ¥ãã
100åã®ãã€ã¢ã«ã«ã次ãã§ãæäœã§è¢«èŠããå
æ¿ãäžæãã€è£ å ¥ããããããã®ãã€ã¢ã«ããäŸ
ãã°æ¯ãšãäžã®æ°ŽæµŽã§0.5æé43âã§æž©çœ®ããã
ãã®æž©çœ®ã®éãå¯Ÿç §åã¯è©Šéšè©Šæäžã«ååšããè
çã¢ã³ãã²ã³ã¯åæ¿äžã®æäœãšçµåããã äžæº¶æ§åæ¿éšæã®å ¥ã€ããã€ã¢ã«ã«é µçŽ æšèã
ããæäœè©Šè¬ãæ·»å ããåã«ãåãã®æž©çœ®ã§åœ¢æ
ããäžå±€æ¶²ãåãé€ããã°ãªããããŸãäžæº¶æ§éš
æãæŽæµããŠæªçµåã¢ã³ãã²ã³ãé€å»ããã°ãªã
ãªãããã®æŽæµæº¶æ¶²ã¯å¥œãŸããã¯ãå¡©åãããªãŠ
ã ã®0.85ïŒ æº¶æ¶²ïŒPH6.5ã7.5ïŒã§ããã該溶液ã
2.5mlãã€çšããŠäºåæŽæµããåŸãåãã€ã¢ã«ã«
æäœâé µçŽ çµåäœ0.3mlãæ·»å ãããããŠå床ã
ãã€ã¢ã«ãæ¯ãšãããªãã43âã§ïŒæé枩眮ã
ããèããŠããã®éãé µçŽ æšèãããæäœã¯ãå
ãã®æž©çœ®ã§æäœè¢«èŠããåæ¿ã«åºå®ãããèçã¢
ã³ãã²ã³ãšåå¿ããã é µçŽ æšèãããæäœè©Šè¬ã®æ·»å ããã³ç¬¬ïŒã®æž©
眮ã®åŸã圢æããäžå±€æ¶²ãªããäžæŸã¿æ¶²ãã¢ã¹ã
ã¬ãŒã¿ãŒã§é€å»ããåãã€ã¢ã«å ã®åæ¿ãæŽæµæº¶
æ¶²2.5mlã®ååéã§ïŒåæŽæµããããã®æŽæµã«ã
ã€ãŠãæªåå¿ã®æäœâé µçŽ çµåäœãé€å»ãããã
次ãã§ãåäžæº¶æ§éšæãæž æµãªãã€ã¢ã«ã«ç§»ãã
ããã«2.5mlã®ïœâãããããšãã«ãã¹ããšãŒã
ïŒpNPPïŒé µçŽ åºè³ªâç·©è¡å€æº¶æ¶²ãæ·»å ããïŒïŒ
mgpNPPïŒmlïŒãïœâãããããšãã«ãã¹ããšãŒ
ãâã¢ã«ã«ãªæ§ãã¹ãã¢ã¿ãŒãŒç³»ã®æé©ãªäœæ¥PH
ã9.8ã§ããã®ã§ãé µçŽ åºè³ªã¯çé žå¡©âMgãç·©è¡
æ¶²ïŒPH9.8±0.1ïŒã«æº¶è§£ãããããã®ç·©è¡æ¶²ãçš
ãããšããããã¯Na2CO30.028Mããã³Mgã
0.001Mã®æ°Žæº¶æ¶²ãããªãã詊éšã»ããã«ã¯ã40
mlã®æ¿åç©ãåãä»ããããããèããŠãããã
èžçæ°Žã§åžéãããšãã¯ã400mgã®pNPPã«çŽæ¥
æ·»å ããããããã®ç·©è¡å€å ¥ãåºè³ªãå ¥ããåŸã
ãã€ã¢ã«ã¯ç¬¬ïŒã®æž©çœ®ã«ä»ããããããã¯ãæ¯ãš
ãããªãã43âã§ïŒæéè¡ããããããå¥ã®æäœ
âé µçŽ çµåäœãçšãããããªããå¥ã®åºè³ªã䜿çš
ããã°ãªããªãã æ¬¡ãã§ãåãã€ã¢ã«ã«2Mã®NaOHæº¶æ¶²ïŒæ»Ž
ïŒ0.1mlïŒãæ·»å ããŠåå¿ã忢ãããããã®ç®ç
ã®ããã«ãå詊éšã»ããã«ã¯3Mã®æ°Žé žåãããª
ãŠã 15mlãåãã€ããããã åæ¿äžã«é µçŽ ãååšããå Žåãããªãã¡ãèè
çã«é¢é£ããã¢ã³ãã²ã³ã«ã€ããŠéœæ§ã®è©Šæãå ¥
ãããã€ã¢ã«å ã§ãäžã«é瀺ããé µçŽ åºè³ªæº¶æ¶²ã¯
ç¡è²ã®æ¶²äœããé»è²ã®æ¶²äœãžãšå€åããã é°æ§å¯Ÿç §ããåŸãããäžæŸã¿æ¶²ãé©åœãªãã€ã¢
ã«å ã«ããŒã«ãããèžçæ°Žããã©ã³ã¯ãšããåå
å 床èšã«ããããã®405nmã§ã®åžåãèªãããã®
é°æ§å¯Ÿç §ã®èªã¿ã600ïŒåžå 床åäœÃ1000ïŒãã
ãå°ãããšããããã¯ã詊éšè©Šæã®çµæãšæ¯èŒã
ãã®ã«é©åœãªåºæºãšèªãããããæ¬¡ãã§ããã®ã
ãŒã«ããé°æ§å¯Ÿç §è©Šæããã©ã³ã¯ãšããŠã詊éšè©Š
æããã³éœæ§å¯Ÿç §ã®å€ãèªã¿ããã®çµæãåžå 床
åäœÃ1000ãšããŠèšé²ãããæãåå å 床èšãçš
ãããšããè©²è£ çœ®ã«é°æ§å¯Ÿç §ãæ¿å ¥ããèªã¿ãé¶
ã«èª¿æŽãäžã€è©Šéšè©Šæã®å€ãçŽæ¥èªãããšãå¯èœ
ã§ãããäžèšããŒã«ããé°æ§å¯Ÿç §è©Šæããã©ã³ã¯
ãšããŠçšãããæªç¥è©Šéšè©Šæã®å åŠå¯åºŠïŒåžå
床ïŒÃ1000ã®å€ã100ãã倧ãããšãã該詊æã¯ã
èèçã«é¢é£ããã¢ã³ãã²ã³ã«ã€ããŠéœæ§ãšèªã
ãããããã®å€ã¯ãéå埩æ§ã®éœæ§ãååšãããª
ãæŠãå®éšå®€æè¡ã®èª€å·®ããçãããšããã®è©²é
å埩æ§éœæ§ãå¶éããããã«éžå®ãããã ãŸãã詊éšè©Šæã®èªã¿ã¯ãåŒ±éœæ§å¯Ÿç §è©Šæãã
ã³åŒ·éœæ§å¯Ÿç §è©Šæãšãæ¯èŒããããšãã§ãããã
ãããŠã詊æäžã®èçã¢ã³ãã²ã³ã®æç¡ã®ã¿ãªã
ããã®æ¿åºŠãç¥ãããšãã§ããã äŸ æªç¥è©Šæã®å矀ã«é¢ããŠäºã€ã®å¯Ÿç §ããªãã¡é°
æ§å¯Ÿç §ïŒãåŒ·éœæ§å¯Ÿç §ïŒãããã³åŒ±éœæ§å¯Ÿç §ïŒã
詊éšãããã¹ãã§ããããããã®å¯Ÿç §ã¯ã詊éšè©Š
æãšåãããã»ã¹ããã³æž©çœ®æéã«ä»ãã¹ãã§ã
ãã æ³šæïŒåç§»åã«ã¯æž æµãªããããåã¯äœ¿ãæšãŠæ«
å£ãçšããŠçžäºæ±æïŒcrossâcontaminationïŒã
é¿ããããšã ïŒ æ°ŽæµŽã43âã«äºãèšå®ããã ïŒ è©Šéšè©Šæããã³å¯Ÿç §ã«å¯Ÿå¿ãããã¹ãïŒçµã®
ãã€ã¢ã«ã«çªå·ãä»ãããããã®ãã€ã¢ã«ãã
ã€ã¢ã«ä¿æåšã«å ¥ããã第ïŒçµã®ãã€ã¢ã«ã¯ã
詊éšè©Šæããã³å¯Ÿç §ãæäœè¢«èŠããåæ¿ããã³
æäœâé µçŽ æšè詊è¬ãšå ±ã«æž©çœ®ããã®ã«çšãã
第ïŒçµã®ãã€ã¢ã«ã¯ãå·¥çš12ã§åºè³ªåå¿ã«çšã
ãã ïŒ ç¬¬ïŒçµã®ãã€ã¢ã«åºã«éЬã°ãããªã³è©Šè¬0.05
mlïŒïŒæ»ŽïŒãããããã§å ¥ãããæå©ãªããš
ã«ã銬ã°ãããªã³ã詊éšè©Šæåžéå€äžã«çšãã
ããšã«ãã€ãŠãæãäººè¡æž äžã«ååšããæäœãš
銬ã°ãããªã³ãšãåå¿ããèããŠè©²æäœããç
ãããšããéç¹ç°æ§æ¬äŒŒéœæ§ãæ¬è³ªäžæé€ãã
ãã ïŒ ãã®ç¬¬ïŒçµã®ãã€ã¢ã«ã«ãå詊éšè©Šæ0.5ml
ãããããã§å ¥ããããã®é該詊æã¯ã察å¿è©Š
æè¡šç€ºããã€ãã€ã¢ã«ã®åºéšã«æ·»å ããããå
æã«ãéœæ§ããã³é°æ§ã®è©Šæ0.5mlã察å¿ãã
åãã€ã¢ã«ã®åºéšã«ããããã§å ¥ããã ïŒ è©²ç¬¬ïŒçµã®åãã€ã¢ã«ã«æäœè¢«èŠããåæ¿äž
æãç§»ãå ¥ãããåæ¿ã®è¡šé¢ã¯æž æµã«ä¿ã€ãè
ããŠãåæ¿ã®ç§»åã¯ãæž æµãªãã³ã»ããåã¯ãµ
ã¯ã·ãšã³ã»ããããã»ã«ããŠãŒã¬ãçšããŠè¡ã
ã¹ãã§ãããåæ¿ãæã§åãæ±ãã¹ãã§ãªãã ïŒ äžèšãã€ã¢ã«ããç©åãªæ¹æãè¡ãã¹ãæ¯ãš
ãä»å±è£ 眮ãåããæ°ŽæµŽã§30åé43âã§æž©çœ®ã
ãã ïŒ å詊æãæäœè¢«èŠããåæ¿ãšå ±ã«æž©çœ®ãã
åŸãåãã€ã¢ã«ããäžæŸã¿æ¶²ãå šãŠå®å šã«ã¢ã¹
ãã¬ãŒãããããã€ã¢ã«å šéšã«ç匵é£å¡©æ°Ž2.5
mlãæ·»å ããŠåæ¿ãæŽæµããããã®æé ãå埩
ããŠååæ¿ãäºåæŽæµãããå šæµäœã®é€å»ãå©
æãã¹ãããã€ã¢ã«ä¿æåšãåŸããªããã¢ã¹ã
ã¬ãŒããããåæŽæµæ¶²ãå ããåŸããã€ã¢ã«ä¿
æåšãæ¯ãšããããã¢ã¹ãã¬ãŒã¿ã«çµåãã容
åšå ã«éãããã廿¶²ã¯ãææ£åã«é«å§èžæ°æ»
èïŒ121âã§æäœïŒæéïŒã«ä»ãã¹ãã§ããã ïŒ æåŸã®æŽæµããã³ã¢ã¹ãã¬ãŒã·ãšã³ã®åŸãå
ãã€ã¢ã«ã«é µçŽ æšèããæäœã®æº¶æ¶²0.3mlãæ·»
å ããã ïŒ ãããã®ãã€ã¢ã«ããç©åã«æ¹æãã¹ãæ¯ãš
ãä»å±è£ 眮ãåããæ°ŽæµŽå ã§ïŒæé43âã§æž©çœ®
ããã 10 ïœâãããããšãã«ãã¹ããšãŒãïŒpNPPïŒ
ïŒ100mgïŒã®ãã€ã¢ã«ïŒåã®å 容ç©ã100mlã®åž
åºè³ªç·©è¡æ¶²ã«ãããå ¥ããããšã«ãããpNPP
åºè³ªã調補ãããïŒãªããåžåºè³ªç·©è¡æ¶²ã¯ãèž
çæ°Ž90mlã«æ¿éçé žãããªãŠã ç·©è¡å€10mlãæ·»
å ããããšã«ãã€ãŠèª¿è£œãããïŒãéãã«å転
ãããŠæ··åãããèããŠã溶液åã¯çŽã¡ã«çèµ·
ãã¹ãã§ããã æ³šæïŒãã®åºè³ªæº¶æ¶²ã¯ã䜿çšãããã®æ¥ã«èª¿è£œã
ã¹ãã§ãããèããŠãäžäœ¿çšæã¯å·åããŠããã¹
ãã§ããã24æéçµéããæº¶æ¶²ã¯å»æ£ãã¹ãã§ã
ãã 11 äžæŸã¿æ¶²ããå·¥çšïŒã®ããã«ã¢ã¹ãã¬ãŒãã
äžã€äžåæŽæµããã 12 å·¥çšïŒã§çšæããçªå·ä»ãã®ã第ïŒçµã®æž æµ
ãªãã€ã¢ã«ã«åæ¿ãç§»ãå ¥ããã 13 åæ¿ãå ¥ããåãã€ã¢ã«ã«ãå·¥çš10ã§èª¿è£œã
ãïœâãããããšãã«ãã¹ããšãŒãåºè³ªæº¶æ¶²ã
2.5mlæ·»å ããã 14 ãããã®ãã€ã¢ã«ããç©åãªæ¹æã®ããæ¯ãš
ãä»å±è£ 眮ãåããæ°ŽæµŽã§ïŒæé43âã§æž©çœ®ã
ãã 15 該ãã€ã¢ã«å šéšã«3Mã®æ°Žé žåãããªãŠã 溶
æ¶²ïŒæ»ŽïŒçŽ0.1mlïŒãæ·»å ããŠåå¿ã忢ãã
ãããã€ã¢ã«ãä¿æåšå ã§æ¯ãšãããŠè©Šè¬ãã
ãæ··åãããåžå 床ã®èªã¿åãã¯ãåå¿åæ¢åŸ
ïŒæéå ã§è¡ãã¹ãã§ããã 16 äžã€ã®é°æ§å¯Ÿç §ãããŒã«ããŠãå 床èšã«ã
ããèžçæ°Žããã©ã³ã¯ãšãã405nmã§ã®åžå 床
ãèªããè©²çµæãåžå 床åäœÃ1000ãšããŠèšé²
ãããããé°æ§å¯Ÿç §ã600ããé«ãå€ã瀺ããª
ãã詊éšã¯äžååã ã€ãããšã«ãªããèããŠè©Š
éšãç¹°è¿ãããªããã°ãªããªãã貫æµåŒå 床èš
ãçšãäžã€åŒ·éœæ§è©Šæã«ééãããšãã¯ã次ã®
詊æã®åžå 床ãèªãåã«ããŠããããªããå¹³ç¿
ãèžçæ°Žã§ãããã¹ãã§ãããäœ¿ãæšãŠã§ãªã
ããŠããããçšãããšãã¯ãéœæ§ã®èªã¿ã«ç¶ã
ãŠè©²ããŠããããèžçæ°Žã§ããããã°ãªããª
ãã 17 ããŒã«ããé°æ§å¯Ÿç §ã®å Žåãè£ çœ®ãé¶ã®åžå
床ã«èª¿æŽãããååå¿æ··åç©ã®åžå åºŠãæž¬å®
ãããã®çµæãåžå 床åäœÃ1000ãšããŠèšé²ã
ããããããªãããæãå 床èšãçšãããšãã
ãã®è£ 眮ãé¶ã«èª¿æŽããããšãã§ããªãããã®
å Žåãé°æ§å¯Ÿç §ã®èªã¿ãå詊æã®èªã¿ããå·®ã
åŒããã°ãªããªãã çµæã®è©äŸ¡ æªç¥ã®è©Šéšè©Šæã®åžå 床åäœÃ1000ã®å€ããã
ãŒã«ããé°æ§å¯Ÿç §ããã©ã³ã¯ãšããŠã100ããé«
ããšãã該詊éšè©Šæã¯åå¿æ§ã§ãããšèªããã
ããåå¿æ§ãšèªãããã詊æã«ã€ããŠã¯è©Šéšãå
埩ããããšãæãŸãããããããªããåå¿æ§è¡æž
ããèèçã«é¢é£ããã¢ã³ãã²ã³ã«ã€ããŠéœæ§ãš
åé¡ããåã«ãã³ãŒãã€ã¹ã»ã³ã³ãã¢ãŒãããªã»
ãã¹ãã»ã»ããïŒCordis Confirmatory Test
SetïŒã§è©ŠéšããŠç¢ºèªãåŸãå¿ èŠãããããã®è©Š
éšã¯åå¿æ§è©Šæå šãŠã«ã€ããŠè¡ããªããã°ãªããª
ãã銬ã°ãããªã³ãšã®äžåã«ãã€ãŠç¢ºèªãããå
å¿æ§è¡æž ã¯ãèèçã¢ã³ãã²ã³ã«ã€ããŠéœæ§ã§
ãããšèªããªããã°ãªããªããäžè¿°ã®åŠãã確èª
詊éšã¯ãçµæãè©äŸ¡ããããã«å¿ èŠã§ãããããŸ
ãããã¯ãé£åå»è¬ç®¡çå±ãå€ãã®å·ã®æ³åŸã§èŠ
æ±ãããŠããããæŠæ¬ããã«ãæ¬çºæã«åŸã€ã確
èªè©Šéšã¯æ¬¡ã®ããã«ããŠéè¡ãããã ããªãã¡ãéœæ§è©Šéšè©Šæã¯äºåºŠè©Šéšãããåã
ã€ã¢ã«å ã®åæ¿ã«è©²è©Šæããããç¬¬ïŒæ®µéã®åŸã
æŽæµã«åŒç¶ããŠãäžæ¹ã®åæ¿ãèçã¢ã³ãã²ã³ã«
察ãç¹ç°æ§ã®æäœã«æŽé²ãããŸãä»ã®åæ¿ãæ£åžž
éŠ¬è¡æž ã«æŽé²ããã30åéæž©çœ®ããããã®åŸã®æ
é ã¯ãæ¥åžžéžå¥è©Šéšã§è¡ã€ãŠããã®ãšåãã§ã
ãããã詊æãèèçã«ã€ããŠéœæ§ã§ãããªãã
銬æäœã«æŽé²ããã詊æã¯ããããŠäœãå€ã瀺ã
ã®ã«ïŒæ£åžžéŠ¬è¡æž ã«æŽé²ãããåæ¿ã¯ãæ¥åžžéžå¥
詊éšã§èŠåºãããã®ãšåçã®é«ãå€ã瀺ãã äœæ¿åºŠã®èçã¢ã³ãã²ã³ãæãã詊æã¯æçµèª
ã¿åãå€ãäœããªããã¡ã§ããã髿¿åºŠã®èçã¢
ã³ãã²ã³ãæãã詊æã¯æå€§å€ã瀺ããæ¿åºŠå·®ã
çãç¯å²ã§ããã°ãè©²çµæèªã¿åãããå åŠå¯åºŠ
ã¯ã詊æäžã«ååšããèçã¢ã³ãã²ã³ã®æ¿åºŠã«é¢
ããå®éæ å ±ãäžããåŸåãããã æ¹æ³ã®éç ïŒ éå埩æ§åå¿æ§ïŒããæãåå¿æ§è©Šæã«é¢ã
ãå埩詊éšã100åãåãå€ããå°ããªå€ã瀺
ããªãããã®è©Šéšã¯éå埩æ§åå¿æ§ã§ãããšæš
å®ããäžã€èèçã«é¢é£ããã¢ã³ãã²ã³ã«ã€ã
ãŠé°æ§ãšèªãããããæåã®çµæã¯ãäžååãª
æŽæµã®åŠãæè¡äžã®èª€å·®ã®ãããããããªãã ïŒ éç¹ç°æ§éœæ§ïŒæãäººè¡æž äžã«ååšããã銬
ã°ãããªã³ã«å¯Ÿãåå¿æ§ã®æäœããçããéç¹
ç°æ§éœæ§ã¯ã詊éšè©Šæåžéå€äžã®éЬã°ãããªã³
ãçšããããšã«ãã€ãŠæ¬è³ªäžæé€ãããã ïŒ EDTAäžã«éããããè¡æ¶²ããã®è¡ãããã¯
䜿çšãã¹ãã§ãªãã æ³šç®ãã¹ãããšã¯ãå皮工çšã§ã®æž©çœ®æéãã
ã³æž©çœ®æž©åºŠãåºãç¯å²ã«ããããããšããããšã§
ãããããããŠãæ¬çºæã¯ãæž©çœ®ã®æéããã³æž©
床ã«é¢ããããªãæ æ§ã«ãéå®ãããã€ããã¯ãª
ããäŸãã°ãäžæº¶æ§éšæã詊æãšå ±ã«æž©çœ®ããæ
éã¯10åã24æéã®ç¯å²ã§ããåŸããŸããã®æž©åºŠ
ã¯ïŒâã50âã®ç¯å²ã§ããããã仿¹ãäžæº¶æ§éš
æãé µçŽ æšèãããçµåçžæãšå ±ã«æž©çœ®ããæé
ã¯30åã24æéã§ããããã®æž©åºŠã¯ïŒâã50âã§
ããã ãŸããæ³šç®ãã¹ãããšã¯ãæ¬çºæã®æ¹æ³ãèè
çã«é¢é£ããã¢ã³ãã²ã³ã®ååšããã©ããªäœæ¶²ã«
ãçšããããŠè©²ã¢ã³ãã²ã³ã®ååšãæ€åºããããš
ãã§ãããšããããšã§ãããããããŠãæ¬æ¹æ³
ã¯ãè¡æž ãè¡ããããè¡ãããã®æåãè¡æž ã®æ
åãå°¿ãåŸæ¶²ããã³è³è髿¶²äžã®èçã¢ã³ãã²ã³
ã®æç¡ã決å®ããã®ã«çšããããšãã§ããã çµ æ æ¬çºæã«åŸã€ãŠå®æœãã詊éšçµæã次衚ã«ç€º
ãã衚ã«ç€ºããçµæã¯ãCEPæ¹æ³ã«ããèç
ã«ã€ããŠéœæ§ãšåºã詊æã«é¢ããæ¬çºæã«åŸã€ã
詊éšçµæã§ããã衚ã«ç€ºããçµæã¯ãæŸå°å ç«
詊éšã§ã¯èçã«ã€ããŠéœæ§ã§ãã€ããCEPæ¹æ³
ã§ã¯é°æ§ãšåºã詊éšè©Šæã«é¢ããå€ã§ããã衚
ã«ç€ºããå€ã¯ãæŸå°å ç«è©ŠéšãCEPäž¡æ¹æ³ã§è
çã«ã€ããŠé°æ§ãšåºã詊éšè©Šæã®çµæã§ããã衚
ïŒïŒ¢ããã³ïŒ£ã«ç€ºããçš®ã ã®è©Šæã«é¢ããæ°å€
ã¯ãæ¬çºæã®æ¹æ³ã§è©ŠéšããŠåŸãããå€ã§ããã
äžè¡šã«ç€ºããåºè³ªã¯å šãŠææ©ããé žãšã¹ãã«ã§
ããã奜ãŸããé µçŽ ã¢ã«ã«ãªæ§ãã¹ãã¢ã¿ãŒãŒã«
åã€ãåºè³ªãšããŠä»ã®ææ©ããé žãšã¹ãã«ã䜿çš
ããããããšã¯æããã§ãããæŽã«ã泚æãã¹ã
ããšã¯ãã¢ã«ã«ãªæ§ãã¹ãã¢ã¿ãŒãŒä»¥å€ã®é µçŽ ã
æäœâé µçŽ çµåäœã«çšãããšãããé©åœãªåºè³ªã
éžå®ããããšã¯åœæ¥è ã«ãšã€ãŠã»ãšãã©å°é£ã§ãª
ããšããããšã§ããã  æ¹ æ³ è©Šéšãæ¬çºæã«åŸã€ãŠè¡ãã¹ãããã€ã¢ã«100
åãæ¶ã§åºåãããçš®ã ã®è©Šæã«å¯Ÿå¿ããããã«
ããããèå¥ãããåãã€ã¢ã«ã«éЬã°ãããªã³æº¶
æ¶²0.05mlãå ¥ããæ¬¡ãã§95åã®ãã€ã¢ã«ã«è©Šéšè©Š
æ0.5mlãæ·»å ãããåæã«ãä»ã®ãã€ã¢ã«ïŒå
ã«å€«ã ãé°æ§å¯Ÿç §è¡æž ã®è©Šæ0.5mlãæ·»å ããä»
ã®ïŒåã«åŒ·éœæ§å¯Ÿç §è¡æž ã®è©Šæ0.5mlãå ¥ããã
ããŠæ®ãïŒåã«ã¯åŒ±éœæ§å¯Ÿç §è¡æž ã®è©Šæ0.5mlã
å ¥ãããããã銬ã°ãããªã³ãšå詊æãå ¥ãã
100åã®ãã€ã¢ã«ã«ã次ãã§ãæäœã§è¢«èŠããå
æ¿ãäžæãã€è£ å ¥ããããããã®ãã€ã¢ã«ããäŸ
ãã°æ¯ãšãäžã®æ°ŽæµŽã§0.5æé43âã§æž©çœ®ããã
ãã®æž©çœ®ã®éãå¯Ÿç §åã¯è©Šéšè©Šæäžã«ååšããè
çã¢ã³ãã²ã³ã¯åæ¿äžã®æäœãšçµåããã äžæº¶æ§åæ¿éšæã®å ¥ã€ããã€ã¢ã«ã«é µçŽ æšèã
ããæäœè©Šè¬ãæ·»å ããåã«ãåãã®æž©çœ®ã§åœ¢æ
ããäžå±€æ¶²ãåãé€ããã°ãªããããŸãäžæº¶æ§éš
æãæŽæµããŠæªçµåã¢ã³ãã²ã³ãé€å»ããã°ãªã
ãªãããã®æŽæµæº¶æ¶²ã¯å¥œãŸããã¯ãå¡©åãããªãŠ
ã ã®0.85ïŒ æº¶æ¶²ïŒPH6.5ã7.5ïŒã§ããã該溶液ã
2.5mlãã€çšããŠäºåæŽæµããåŸãåãã€ã¢ã«ã«
æäœâé µçŽ çµåäœ0.3mlãæ·»å ãããããŠå床ã
ãã€ã¢ã«ãæ¯ãšãããªãã43âã§ïŒæé枩眮ã
ããèããŠããã®éãé µçŽ æšèãããæäœã¯ãå
ãã®æž©çœ®ã§æäœè¢«èŠããåæ¿ã«åºå®ãããèçã¢
ã³ãã²ã³ãšåå¿ããã é µçŽ æšèãããæäœè©Šè¬ã®æ·»å ããã³ç¬¬ïŒã®æž©
眮ã®åŸã圢æããäžå±€æ¶²ãªããäžæŸã¿æ¶²ãã¢ã¹ã
ã¬ãŒã¿ãŒã§é€å»ããåãã€ã¢ã«å ã®åæ¿ãæŽæµæº¶
æ¶²2.5mlã®ååéã§ïŒåæŽæµããããã®æŽæµã«ã
ã€ãŠãæªåå¿ã®æäœâé µçŽ çµåäœãé€å»ãããã
次ãã§ãåäžæº¶æ§éšæãæž æµãªãã€ã¢ã«ã«ç§»ãã
ããã«2.5mlã®ïœâãããããšãã«ãã¹ããšãŒã
ïŒpNPPïŒé µçŽ åºè³ªâç·©è¡å€æº¶æ¶²ãæ·»å ããïŒïŒ
mgpNPPïŒmlïŒãïœâãããããšãã«ãã¹ããšãŒ
ãâã¢ã«ã«ãªæ§ãã¹ãã¢ã¿ãŒãŒç³»ã®æé©ãªäœæ¥PH
ã9.8ã§ããã®ã§ãé µçŽ åºè³ªã¯çé žå¡©âMgãç·©è¡
æ¶²ïŒPH9.8±0.1ïŒã«æº¶è§£ãããããã®ç·©è¡æ¶²ãçš
ãããšããããã¯Na2CO30.028Mããã³Mgã
0.001Mã®æ°Žæº¶æ¶²ãããªãã詊éšã»ããã«ã¯ã40
mlã®æ¿åç©ãåãä»ããããããèããŠãããã
èžçæ°Žã§åžéãããšãã¯ã400mgã®pNPPã«çŽæ¥
æ·»å ããããããã®ç·©è¡å€å ¥ãåºè³ªãå ¥ããåŸã
ãã€ã¢ã«ã¯ç¬¬ïŒã®æž©çœ®ã«ä»ããããããã¯ãæ¯ãš
ãããªãã43âã§ïŒæéè¡ããããããå¥ã®æäœ
âé µçŽ çµåäœãçšãããããªããå¥ã®åºè³ªã䜿çš
ããã°ãªããªãã æ¬¡ãã§ãåãã€ã¢ã«ã«2Mã®NaOHæº¶æ¶²ïŒæ»Ž
ïŒ0.1mlïŒãæ·»å ããŠåå¿ã忢ãããããã®ç®ç
ã®ããã«ãå詊éšã»ããã«ã¯3Mã®æ°Žé žåãããª
ãŠã 15mlãåãã€ããããã åæ¿äžã«é µçŽ ãååšããå Žåãããªãã¡ãèè
çã«é¢é£ããã¢ã³ãã²ã³ã«ã€ããŠéœæ§ã®è©Šæãå ¥
ãããã€ã¢ã«å ã§ãäžã«é瀺ããé µçŽ åºè³ªæº¶æ¶²ã¯
ç¡è²ã®æ¶²äœããé»è²ã®æ¶²äœãžãšå€åããã é°æ§å¯Ÿç §ããåŸãããäžæŸã¿æ¶²ãé©åœãªãã€ã¢
ã«å ã«ããŒã«ãããèžçæ°Žããã©ã³ã¯ãšããåå
å 床èšã«ããããã®405nmã§ã®åžåãèªãããã®
é°æ§å¯Ÿç §ã®èªã¿ã600ïŒåžå 床åäœÃ1000ïŒãã
ãå°ãããšããããã¯ã詊éšè©Šæã®çµæãšæ¯èŒã
ãã®ã«é©åœãªåºæºãšèªãããããæ¬¡ãã§ããã®ã
ãŒã«ããé°æ§å¯Ÿç §è©Šæããã©ã³ã¯ãšããŠã詊éšè©Š
æããã³éœæ§å¯Ÿç §ã®å€ãèªã¿ããã®çµæãåžå 床
åäœÃ1000ãšããŠèšé²ãããæãåå å 床èšãçš
ãããšããè©²è£ çœ®ã«é°æ§å¯Ÿç §ãæ¿å ¥ããèªã¿ãé¶
ã«èª¿æŽãäžã€è©Šéšè©Šæã®å€ãçŽæ¥èªãããšãå¯èœ
ã§ãããäžèšããŒã«ããé°æ§å¯Ÿç §è©Šæããã©ã³ã¯
ãšããŠçšãããæªç¥è©Šéšè©Šæã®å åŠå¯åºŠïŒåžå
床ïŒÃ1000ã®å€ã100ãã倧ãããšãã該詊æã¯ã
èèçã«é¢é£ããã¢ã³ãã²ã³ã«ã€ããŠéœæ§ãšèªã
ãããããã®å€ã¯ãéå埩æ§ã®éœæ§ãååšãããª
ãæŠãå®éšå®€æè¡ã®èª€å·®ããçãããšããã®è©²é
å埩æ§éœæ§ãå¶éããããã«éžå®ãããã ãŸãã詊éšè©Šæã®èªã¿ã¯ãåŒ±éœæ§å¯Ÿç §è©Šæãã
ã³åŒ·éœæ§å¯Ÿç §è©Šæãšãæ¯èŒããããšãã§ãããã
ãããŠã詊æäžã®èçã¢ã³ãã²ã³ã®æç¡ã®ã¿ãªã
ããã®æ¿åºŠãç¥ãããšãã§ããã äŸ æªç¥è©Šæã®å矀ã«é¢ããŠäºã€ã®å¯Ÿç §ããªãã¡é°
æ§å¯Ÿç §ïŒãåŒ·éœæ§å¯Ÿç §ïŒãããã³åŒ±éœæ§å¯Ÿç §ïŒã
詊éšãããã¹ãã§ããããããã®å¯Ÿç §ã¯ã詊éšè©Š
æãšåãããã»ã¹ããã³æž©çœ®æéã«ä»ãã¹ãã§ã
ãã æ³šæïŒåç§»åã«ã¯æž æµãªããããåã¯äœ¿ãæšãŠæ«
å£ãçšããŠçžäºæ±æïŒcrossâcontaminationïŒã
é¿ããããšã ïŒ æ°ŽæµŽã43âã«äºãèšå®ããã ïŒ è©Šéšè©Šæããã³å¯Ÿç §ã«å¯Ÿå¿ãããã¹ãïŒçµã®
ãã€ã¢ã«ã«çªå·ãä»ãããããã®ãã€ã¢ã«ãã
ã€ã¢ã«ä¿æåšã«å ¥ããã第ïŒçµã®ãã€ã¢ã«ã¯ã
詊éšè©Šæããã³å¯Ÿç §ãæäœè¢«èŠããåæ¿ããã³
æäœâé µçŽ æšè詊è¬ãšå ±ã«æž©çœ®ããã®ã«çšãã
第ïŒçµã®ãã€ã¢ã«ã¯ãå·¥çš12ã§åºè³ªåå¿ã«çšã
ãã ïŒ ç¬¬ïŒçµã®ãã€ã¢ã«åºã«éЬã°ãããªã³è©Šè¬0.05
mlïŒïŒæ»ŽïŒãããããã§å ¥ãããæå©ãªããš
ã«ã銬ã°ãããªã³ã詊éšè©Šæåžéå€äžã«çšãã
ããšã«ãã€ãŠãæãäººè¡æž äžã«ååšããæäœãš
銬ã°ãããªã³ãšãåå¿ããèããŠè©²æäœããç
ãããšããéç¹ç°æ§æ¬äŒŒéœæ§ãæ¬è³ªäžæé€ãã
ãã ïŒ ãã®ç¬¬ïŒçµã®ãã€ã¢ã«ã«ãå詊éšè©Šæ0.5ml
ãããããã§å ¥ããããã®é該詊æã¯ã察å¿è©Š
æè¡šç€ºããã€ãã€ã¢ã«ã®åºéšã«æ·»å ããããå
æã«ãéœæ§ããã³é°æ§ã®è©Šæ0.5mlã察å¿ãã
åãã€ã¢ã«ã®åºéšã«ããããã§å ¥ããã ïŒ è©²ç¬¬ïŒçµã®åãã€ã¢ã«ã«æäœè¢«èŠããåæ¿äž
æãç§»ãå ¥ãããåæ¿ã®è¡šé¢ã¯æž æµã«ä¿ã€ãè
ããŠãåæ¿ã®ç§»åã¯ãæž æµãªãã³ã»ããåã¯ãµ
ã¯ã·ãšã³ã»ããããã»ã«ããŠãŒã¬ãçšããŠè¡ã
ã¹ãã§ãããåæ¿ãæã§åãæ±ãã¹ãã§ãªãã ïŒ äžèšãã€ã¢ã«ããç©åãªæ¹æãè¡ãã¹ãæ¯ãš
ãä»å±è£ 眮ãåããæ°ŽæµŽã§30åé43âã§æž©çœ®ã
ãã ïŒ å詊æãæäœè¢«èŠããåæ¿ãšå ±ã«æž©çœ®ãã
åŸãåãã€ã¢ã«ããäžæŸã¿æ¶²ãå šãŠå®å šã«ã¢ã¹
ãã¬ãŒãããããã€ã¢ã«å šéšã«ç匵é£å¡©æ°Ž2.5
mlãæ·»å ããŠåæ¿ãæŽæµããããã®æé ãå埩
ããŠååæ¿ãäºåæŽæµãããå šæµäœã®é€å»ãå©
æãã¹ãããã€ã¢ã«ä¿æåšãåŸããªããã¢ã¹ã
ã¬ãŒããããåæŽæµæ¶²ãå ããåŸããã€ã¢ã«ä¿
æåšãæ¯ãšããããã¢ã¹ãã¬ãŒã¿ã«çµåãã容
åšå ã«éãããã廿¶²ã¯ãææ£åã«é«å§èžæ°æ»
èïŒ121âã§æäœïŒæéïŒã«ä»ãã¹ãã§ããã ïŒ æåŸã®æŽæµããã³ã¢ã¹ãã¬ãŒã·ãšã³ã®åŸãå
ãã€ã¢ã«ã«é µçŽ æšèããæäœã®æº¶æ¶²0.3mlãæ·»
å ããã ïŒ ãããã®ãã€ã¢ã«ããç©åã«æ¹æãã¹ãæ¯ãš
ãä»å±è£ 眮ãåããæ°ŽæµŽå ã§ïŒæé43âã§æž©çœ®
ããã 10 ïœâãããããšãã«ãã¹ããšãŒãïŒpNPPïŒ
ïŒ100mgïŒã®ãã€ã¢ã«ïŒåã®å 容ç©ã100mlã®åž
åºè³ªç·©è¡æ¶²ã«ãããå ¥ããããšã«ãããpNPP
åºè³ªã調補ãããïŒãªããåžåºè³ªç·©è¡æ¶²ã¯ãèž
çæ°Ž90mlã«æ¿éçé žãããªãŠã ç·©è¡å€10mlãæ·»
å ããããšã«ãã€ãŠèª¿è£œãããïŒãéãã«å転
ãããŠæ··åãããèããŠã溶液åã¯çŽã¡ã«çèµ·
ãã¹ãã§ããã æ³šæïŒãã®åºè³ªæº¶æ¶²ã¯ã䜿çšãããã®æ¥ã«èª¿è£œã
ã¹ãã§ãããèããŠãäžäœ¿çšæã¯å·åããŠããã¹
ãã§ããã24æéçµéããæº¶æ¶²ã¯å»æ£ãã¹ãã§ã
ãã 11 äžæŸã¿æ¶²ããå·¥çšïŒã®ããã«ã¢ã¹ãã¬ãŒãã
äžã€äžåæŽæµããã 12 å·¥çšïŒã§çšæããçªå·ä»ãã®ã第ïŒçµã®æž æµ
ãªãã€ã¢ã«ã«åæ¿ãç§»ãå ¥ããã 13 åæ¿ãå ¥ããåãã€ã¢ã«ã«ãå·¥çš10ã§èª¿è£œã
ãïœâãããããšãã«ãã¹ããšãŒãåºè³ªæº¶æ¶²ã
2.5mlæ·»å ããã 14 ãããã®ãã€ã¢ã«ããç©åãªæ¹æã®ããæ¯ãš
ãä»å±è£ 眮ãåããæ°ŽæµŽã§ïŒæé43âã§æž©çœ®ã
ãã 15 該ãã€ã¢ã«å šéšã«3Mã®æ°Žé žåãããªãŠã 溶
æ¶²ïŒæ»ŽïŒçŽ0.1mlïŒãæ·»å ããŠåå¿ã忢ãã
ãããã€ã¢ã«ãä¿æåšå ã§æ¯ãšãããŠè©Šè¬ãã
ãæ··åãããåžå 床ã®èªã¿åãã¯ãåå¿åæ¢åŸ
ïŒæéå ã§è¡ãã¹ãã§ããã 16 äžã€ã®é°æ§å¯Ÿç §ãããŒã«ããŠãå 床èšã«ã
ããèžçæ°Žããã©ã³ã¯ãšãã405nmã§ã®åžå 床
ãèªããè©²çµæãåžå 床åäœÃ1000ãšããŠèšé²
ãããããé°æ§å¯Ÿç §ã600ããé«ãå€ã瀺ããª
ãã詊éšã¯äžååã ã€ãããšã«ãªããèããŠè©Š
éšãç¹°è¿ãããªããã°ãªããªãã貫æµåŒå 床èš
ãçšãäžã€åŒ·éœæ§è©Šæã«ééãããšãã¯ã次ã®
詊æã®åžå 床ãèªãåã«ããŠããããªããå¹³ç¿
ãèžçæ°Žã§ãããã¹ãã§ãããäœ¿ãæšãŠã§ãªã
ããŠããããçšãããšãã¯ãéœæ§ã®èªã¿ã«ç¶ã
ãŠè©²ããŠããããèžçæ°Žã§ããããã°ãªããª
ãã 17 ããŒã«ããé°æ§å¯Ÿç §ã®å Žåãè£ çœ®ãé¶ã®åžå
床ã«èª¿æŽãããååå¿æ··åç©ã®åžå åºŠãæž¬å®
ãããã®çµæãåžå 床åäœÃ1000ãšããŠèšé²ã
ããããããªãããæãå 床èšãçšãããšãã
ãã®è£ 眮ãé¶ã«èª¿æŽããããšãã§ããªãããã®
å Žåãé°æ§å¯Ÿç §ã®èªã¿ãå詊æã®èªã¿ããå·®ã
åŒããã°ãªããªãã çµæã®è©äŸ¡ æªç¥ã®è©Šéšè©Šæã®åžå 床åäœÃ1000ã®å€ããã
ãŒã«ããé°æ§å¯Ÿç §ããã©ã³ã¯ãšããŠã100ããé«
ããšãã該詊éšè©Šæã¯åå¿æ§ã§ãããšèªããã
ããåå¿æ§ãšèªãããã詊æã«ã€ããŠã¯è©Šéšãå
埩ããããšãæãŸãããããããªããåå¿æ§è¡æž
ããèèçã«é¢é£ããã¢ã³ãã²ã³ã«ã€ããŠéœæ§ãš
åé¡ããåã«ãã³ãŒãã€ã¹ã»ã³ã³ãã¢ãŒãããªã»
ãã¹ãã»ã»ããïŒCordis Confirmatory Test
SetïŒã§è©ŠéšããŠç¢ºèªãåŸãå¿ èŠãããããã®è©Š
éšã¯åå¿æ§è©Šæå šãŠã«ã€ããŠè¡ããªããã°ãªããª
ãã銬ã°ãããªã³ãšã®äžåã«ãã€ãŠç¢ºèªãããå
å¿æ§è¡æž ã¯ãèèçã¢ã³ãã²ã³ã«ã€ããŠéœæ§ã§
ãããšèªããªããã°ãªããªããäžè¿°ã®åŠãã確èª
詊éšã¯ãçµæãè©äŸ¡ããããã«å¿ èŠã§ãããããŸ
ãããã¯ãé£åå»è¬ç®¡çå±ãå€ãã®å·ã®æ³åŸã§èŠ
æ±ãããŠããããæŠæ¬ããã«ãæ¬çºæã«åŸã€ã確
èªè©Šéšã¯æ¬¡ã®ããã«ããŠéè¡ãããã ããªãã¡ãéœæ§è©Šéšè©Šæã¯äºåºŠè©Šéšãããåã
ã€ã¢ã«å ã®åæ¿ã«è©²è©Šæããããç¬¬ïŒæ®µéã®åŸã
æŽæµã«åŒç¶ããŠãäžæ¹ã®åæ¿ãèçã¢ã³ãã²ã³ã«
察ãç¹ç°æ§ã®æäœã«æŽé²ãããŸãä»ã®åæ¿ãæ£åžž
éŠ¬è¡æž ã«æŽé²ããã30åéæž©çœ®ããããã®åŸã®æ
é ã¯ãæ¥åžžéžå¥è©Šéšã§è¡ã€ãŠããã®ãšåãã§ã
ãããã詊æãèèçã«ã€ããŠéœæ§ã§ãããªãã
銬æäœã«æŽé²ããã詊æã¯ããããŠäœãå€ã瀺ã
ã®ã«ïŒæ£åžžéŠ¬è¡æž ã«æŽé²ãããåæ¿ã¯ãæ¥åžžéžå¥
詊éšã§èŠåºãããã®ãšåçã®é«ãå€ã瀺ãã äœæ¿åºŠã®èçã¢ã³ãã²ã³ãæãã詊æã¯æçµèª
ã¿åãå€ãäœããªããã¡ã§ããã髿¿åºŠã®èçã¢
ã³ãã²ã³ãæãã詊æã¯æå€§å€ã瀺ããæ¿åºŠå·®ã
çãç¯å²ã§ããã°ãè©²çµæèªã¿åãããå åŠå¯åºŠ
ã¯ã詊æäžã«ååšããèçã¢ã³ãã²ã³ã®æ¿åºŠã«é¢
ããå®éæ å ±ãäžããåŸåãããã æ¹æ³ã®éç ïŒ éå埩æ§åå¿æ§ïŒããæãåå¿æ§è©Šæã«é¢ã
ãå埩詊éšã100åãåãå€ããå°ããªå€ã瀺
ããªãããã®è©Šéšã¯éå埩æ§åå¿æ§ã§ãããšæš
å®ããäžã€èèçã«é¢é£ããã¢ã³ãã²ã³ã«ã€ã
ãŠé°æ§ãšèªãããããæåã®çµæã¯ãäžååãª
æŽæµã®åŠãæè¡äžã®èª€å·®ã®ãããããããªãã ïŒ éç¹ç°æ§éœæ§ïŒæãäººè¡æž äžã«ååšããã銬
ã°ãããªã³ã«å¯Ÿãåå¿æ§ã®æäœããçããéç¹
ç°æ§éœæ§ã¯ã詊éšè©Šæåžéå€äžã®éЬã°ãããªã³
ãçšããããšã«ãã€ãŠæ¬è³ªäžæé€ãããã ïŒ EDTAäžã«éããããè¡æ¶²ããã®è¡ãããã¯
䜿çšãã¹ãã§ãªãã æ³šç®ãã¹ãããšã¯ãå皮工çšã§ã®æž©çœ®æéãã
ã³æž©çœ®æž©åºŠãåºãç¯å²ã«ããããããšããããšã§
ãããããããŠãæ¬çºæã¯ãæž©çœ®ã®æéããã³æž©
床ã«é¢ããããªãæ æ§ã«ãéå®ãããã€ããã¯ãª
ããäŸãã°ãäžæº¶æ§éšæã詊æãšå ±ã«æž©çœ®ããæ
éã¯10åã24æéã®ç¯å²ã§ããåŸããŸããã®æž©åºŠ
ã¯ïŒâã50âã®ç¯å²ã§ããããã仿¹ãäžæº¶æ§éš
æãé µçŽ æšèãããçµåçžæãšå ±ã«æž©çœ®ããæé
ã¯30åã24æéã§ããããã®æž©åºŠã¯ïŒâã50âã§
ããã ãŸããæ³šç®ãã¹ãããšã¯ãæ¬çºæã®æ¹æ³ãèè
çã«é¢é£ããã¢ã³ãã²ã³ã®ååšããã©ããªäœæ¶²ã«
ãçšããããŠè©²ã¢ã³ãã²ã³ã®ååšãæ€åºããããš
ãã§ãããšããããšã§ãããããããŠãæ¬æ¹æ³
ã¯ãè¡æž ãè¡ããããè¡ãããã®æåãè¡æž ã®æ
åãå°¿ãåŸæ¶²ããã³è³è髿¶²äžã®èçã¢ã³ãã²ã³
ã®æç¡ã決å®ããã®ã«çšããããšãã§ããã çµ æ æ¬çºæã«åŸã€ãŠå®æœãã詊éšçµæã次衚ã«ç€º
ãã衚ã«ç€ºããçµæã¯ãCEPæ¹æ³ã«ããèç
ã«ã€ããŠéœæ§ãšåºã詊æã«é¢ããæ¬çºæã«åŸã€ã
詊éšçµæã§ããã衚ã«ç€ºããçµæã¯ãæŸå°å ç«
詊éšã§ã¯èçã«ã€ããŠéœæ§ã§ãã€ããCEPæ¹æ³
ã§ã¯é°æ§ãšåºã詊éšè©Šæã«é¢ããå€ã§ããã衚
ã«ç€ºããå€ã¯ãæŸå°å ç«è©ŠéšãCEPäž¡æ¹æ³ã§è
çã«ã€ããŠé°æ§ãšåºã詊éšè©Šæã®çµæã§ããã衚
ïŒïŒ¢ããã³ïŒ£ã«ç€ºããçš®ã ã®è©Šæã«é¢ããæ°å€
ã¯ãæ¬çºæã®æ¹æ³ã§è©ŠéšããŠåŸãããå€ã§ããã
ã衚ã
æ¬çºæã¯ããã®ç²Ÿç¥ãªããäžå¯æ¬ ãªç¹åŸŽãéžè±
ããããšãªãä»ã®ç¹å®æ æ§ã§å ·äœåããããšãã§
ãããããæ ãæ€åŠã«èšèŒããå ·äœåã¯ãäžã®èª¬
æããã¯ããããç¹èš±è«æ±ã®ç¯å²ãã«ãã€ãŠç€ºã
ããæ¬çºæã®ç¯å²ããå šãŠã®ç¹ã§ãäŸç€ºãããã®
ã§ããéå®ãããã®ã§ã¯ãªããšèªããããã¹ãã§
ããã
ããããšãªãä»ã®ç¹å®æ æ§ã§å ·äœåããããšãã§
ãããããæ ãæ€åŠã«èšèŒããå ·äœåã¯ãäžã®èª¬
æããã¯ããããç¹èš±è«æ±ã®ç¯å²ãã«ãã€ãŠç€ºã
ããæ¬çºæã®ç¯å²ããå šãŠã®ç¹ã§ãäŸç€ºãããã®
ã§ããéå®ãããã®ã§ã¯ãªããšèªããããã¹ãã§
ããã
第ïŒå³ã¯ãæ¬çºæã®æ¹æ³ã®å·¥çšãèŠçŽããæµã
å³ã§ããã第ïŒå³ã¯ãæ¬çºæã®æ¹æ³ããã³è©Šéšã»
ããã«çšããããéžå®ãããäžæº¶æ§éšæã®éèŠå³
ã§ããã第ïŒå³ïŒç¬¬ïŒå³ããã³ç¬¬ïŒå³ã¯ãæ¬çºæ
ã®å ç«æž¬å®æ³ã®äžã€ã®æž©çœ®æ®µéãå³ç€ºããã第ïŒ
å³ã¯ãæ¬çºæã«åŸã€ãŠè©Šéšããã詊éšè©Šæã®éœæ§
å ç«æž¬å®ã®éã«çŸåºããããµã³ãã€ãããæ§é ã®
æŠç¥ç衚瀺ã§ããã å³äžäž»èŠéšåã瀺ãèšå·ã®èª¬æã¯ä»¥äžã®éãã§
ãããïŒïŒïŒäžæº¶æ§ãããªãã¯ã¹ãïŒïŒïŒæ Œå圢
æš¡æ§ã®è¡šé¢ãïŒïŒïŒãã€ã¢ã«ã
å³ã§ããã第ïŒå³ã¯ãæ¬çºæã®æ¹æ³ããã³è©Šéšã»
ããã«çšããããéžå®ãããäžæº¶æ§éšæã®éèŠå³
ã§ããã第ïŒå³ïŒç¬¬ïŒå³ããã³ç¬¬ïŒå³ã¯ãæ¬çºæ
ã®å ç«æž¬å®æ³ã®äžã€ã®æž©çœ®æ®µéãå³ç€ºããã第ïŒ
å³ã¯ãæ¬çºæã«åŸã€ãŠè©Šéšããã詊éšè©Šæã®éœæ§
å ç«æž¬å®ã®éã«çŸåºããããµã³ãã€ãããæ§é ã®
æŠç¥ç衚瀺ã§ããã å³äžäž»èŠéšåã瀺ãèšå·ã®èª¬æã¯ä»¥äžã®éãã§
ãããïŒïŒïŒäžæº¶æ§ãããªãã¯ã¹ãïŒïŒïŒæ Œå圢
æš¡æ§ã®è¡šé¢ãïŒïŒïŒãã€ã¢ã«ã
Claims (1)
- ãç¹èš±è«æ±ã®ç¯å²ã ïŒ èçã¢ã³ãã²ã³ãå«ãæ¶²äœè©Šæãã該ã¢ã³ã
ã²ã³ãæ€åºããã«åœãã (a) åèšã¢ã³ãã²ã³ãšé¢é£ãã第äžéã®æäœã«ã
ãŠãæ°Žäžæº¶æ§æ°Žéæžæ¿æ§åºäœãã€ãªã¢ãŒã«çµå
ããæäœãçšæãã (b) åèšæ¶²äœè©Šæãåèšå·¥çš(a)ã®åºäœãã€ãªã¢ãŒ
ãšæ¥è§Šæž©çœ®ããŠåå¿æ··åç©ã圢æãã (c) åèšã¢ã³ãã²ã³ãšé¢é£ãã第äºéã®æäœã«ã
ãŠãé µçŽ ãšå ±æçµåããæäœã®æº¶æ¶²ãçšæãã (d) åèšå·¥çš(b)ããååŸããåå¿æ··åç©ãåèšå·¥
çš(c)ã®é µçŽ çµåæäœãšæ¥è§Šæž©çœ®ããé µçŽ çµåæ
äœã®æº¶æ¶²ããåºäœãã€ãªã¢ãŒãåé¢ãã (e) åºäœçžã«çµåããç©è³ªã®é µçŽ æŽ»æ§ããæ€åºã
ã¹ãèçã¢ã³ãã²ã³ã®ååšã«çžé¢ããã諞工çš
ãããªãæ¹æ³ã
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US61774675A | 1975-09-29 | 1975-09-29 | |
| US61774475A | 1975-09-29 | 1975-09-29 | |
| US05/617,745 US4157280A (en) | 1975-09-29 | 1975-09-29 | Test set for detecting the presence of antigens associated with hepatitis |
| US06/617,743 US4474878A (en) | 1975-09-29 | 1975-09-29 | Sandwich EIA for antigen associated with hepatitis |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS5257316A JPS5257316A (en) | 1977-05-11 |
| JPS6224745B2 true JPS6224745B2 (ja) | 1987-05-29 |
Family
ID=27505141
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP11607076A Granted JPS5257316A (en) | 1975-09-29 | 1976-09-29 | Determination of presence of antigen related to hepatisis |
Country Status (7)
| Country | Link |
|---|---|
| JP (1) | JPS5257316A (ja) |
| CA (1) | CA1106281A (ja) |
| DE (1) | DE2643829A1 (ja) |
| FR (1) | FR2325934A1 (ja) |
| GB (1) | GB1571196A (ja) |
| NL (1) | NL7610801A (ja) |
| SE (1) | SE7610683L (ja) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0318011U (ja) * | 1989-07-05 | 1991-02-22 |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2016687B (en) * | 1978-03-20 | 1982-09-08 | Abbott Lab | Sugar coated reagents for solid phase immunoassay |
| US4241175A (en) * | 1978-12-18 | 1980-12-23 | Merck & Co., Inc. | Assay for hepatitis B core antibody |
| CA1148859A (en) * | 1979-06-14 | 1983-06-28 | Lacy R. Overby | Simultaneous assay of two hepatitis viruses using a solid phase |
| ATE42002T1 (de) * | 1983-09-14 | 1989-04-15 | Akzo Nv | Verfahren zur immunochemischen bestimmung von hepatitis-b-core-antigenen. |
| JP3058673B2 (ja) * | 1989-11-10 | 2000-07-04 | æ ªåŒäŒç€Ÿæé»è | ãµã€ãã«ã€ã³ã®æž¬å®æ¹æ³åã³ãã®æž¬å®çšããã |
| KR101799826B1 (ko) * | 2016-09-19 | 2017-11-21 | ë°ìŽì€ë± í¬ ì£Œìíì¬ | ìííê²ì¬ì 멎ìë°ìê²ì¬ë¥Œ ìííë ë©í°ì ë ë° ìŽë¥Œ ìŽì©í ê²ì¬ë°©ë² |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3654090A (en) * | 1968-09-24 | 1972-04-04 | Organon | Method for the determination of antigens and antibodies |
| NL154600B (nl) * | 1971-02-10 | 1977-09-15 | Organon Nv | Werkwijze voor het aantonen en bepalen van specifiek bindende eiwitten en hun corresponderende bindbare stoffen. |
| NL154599B (nl) * | 1970-12-28 | 1977-09-15 | Organon Nv | Werkwijze voor het aantonen en bepalen van specifiek bindende eiwitten en hun corresponderende bindbare stoffen, alsmede testverpakking. |
| US3826619A (en) * | 1971-12-21 | 1974-07-30 | Abbott Lab | Test apparatus for direct radioimmuno-assay for antigens and their antibodies |
| US3867517A (en) * | 1971-12-21 | 1975-02-18 | Abbott Lab | Direct radioimmunoassay for antigens and their antibodies |
| US4039652A (en) * | 1973-10-11 | 1977-08-02 | Miles Laboratories, Inc. | Column method of immunoassay employing an immobilized binding partner |
| US4034072A (en) * | 1975-07-21 | 1977-07-05 | Corning Glass Works | Serum hepatitis test |
-
1976
- 1976-09-27 SE SE7610683A patent/SE7610683L/ unknown
- 1976-09-28 FR FR7629155A patent/FR2325934A1/fr active Granted
- 1976-09-29 NL NL7610801A patent/NL7610801A/xx not_active Application Discontinuation
- 1976-09-29 GB GB4037676A patent/GB1571196A/en not_active Expired
- 1976-09-29 JP JP11607076A patent/JPS5257316A/ja active Granted
- 1976-09-29 CA CA262,333A patent/CA1106281A/en not_active Expired
- 1976-09-29 DE DE19762643829 patent/DE2643829A1/de not_active Ceased
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0318011U (ja) * | 1989-07-05 | 1991-02-22 |
Also Published As
| Publication number | Publication date |
|---|---|
| GB1571196A (en) | 1980-07-09 |
| JPS5257316A (en) | 1977-05-11 |
| CA1106281A (en) | 1981-08-04 |
| FR2325934B1 (ja) | 1982-08-20 |
| NL7610801A (nl) | 1977-03-31 |
| SE7610683L (sv) | 1977-06-10 |
| FR2325934A1 (fr) | 1977-04-22 |
| DE2643829A1 (de) | 1977-04-14 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US4474878A (en) | Sandwich EIA for antigen associated with hepatitis | |
| US4642285A (en) | Sandwich EIA for antigen | |
| US4347311A (en) | Enzyme immunoassay for determining antigen specific antibodies and test kit for carrying out this assay | |
| US4332783A (en) | Process for immunologic determination tests | |
| US4338094A (en) | Macroencapsulated immunosorbent assay technique | |
| EP0217403A2 (en) | Solid-phase analytical device and method for using same | |
| US4157280A (en) | Test set for detecting the presence of antigens associated with hepatitis | |
| EP0468585B1 (en) | Biologically active reagent, analytical element and methods for use of the reagent | |
| US4280816A (en) | Macroencapsulated immunosorbent assay technique and element therefor | |
| CA1148859A (en) | Simultaneous assay of two hepatitis viruses using a solid phase | |
| JP2636331B2 (ja) | æåç¹ç°çãªæäœã®äžæ®µéæž¬å®æ³ããã³ããã«é©ããè©Šè¬ | |
| JPH03502241A (ja) | ããè¡æž ã«ãããïœïœïœæåãæ€åºããé µçŽ å ç«æ€å®æ³ | |
| US4317810A (en) | Waffle-like matrix for immunoassay and preparation thereof | |
| JP3715027B2 (ja) | åèçãŠã€ã«ã¹ææç蚺æè¬ | |
| JP4086266B2 (ja) | å ç«æž¬å®æ¹æ³ | |
| JPS6224745B2 (ja) | ||
| WO1990015328A1 (en) | Improved immunoassay | |
| JPH11248706A (ja) | éç¹ç°çåžç鲿¢å€ | |
| US4242322A (en) | Methods and materials for detecting antigens and antibodies | |
| JPH02124464A (ja) | ç£æ§ããŒã«ãŒç²åãçšããå ç«åŠçæž¬å®æ¹æ³ | |
| JP3547729B2 (ja) | ã¢ãã»ã€ | |
| RU2376604C2 (ru) | СпПÑПб вÑÑÐ²Ð»ÐµÐœÐžÑ Ð°ÐœÑОгеМа ОлО аМÑОÑела в ОЌЌÑМПÑеÑЌеМÑМПЌ аМалОзе | |
| JPH11352127A (ja) | éç¹ç°çåžç鲿¢å€ | |
| JPH09304386A (ja) | å ç«èšºæè¬ã®è£œé æ¹æ³ããã³åŸãããå ç«èšºæè¬ | |
| CA1111764A (en) | Test set for the detection of antigen associated with hepatitis by "sandwich" method |