JPS6248398A - 化学発光法による酵素の定量法 - Google Patents

化学発光法による酵素の定量法

Info

Publication number: JPS6248398A
Authority: JP; Japan
Prior art keywords: fluorescent substance; enzyme; reaction; liberate; added
Prior art date: 1985-08-26
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Granted

Application number

JP18581785A

Other languages

English (en)

Japanese (ja)

Other versions

JPH0544280B2 (fr

Inventor

Haruo Watanabe

治夫渡辺

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Toyobo Co Ltd

Original Assignee

Toyobo Co Ltd

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1985-08-26

Filing date

1985-08-26

Publication date

1987-03-03

1985-08-26 Application filed by Toyobo Co Ltd filed Critical Toyobo Co Ltd

1985-08-26 Priority to JP18581785A priority Critical patent/JPS6248398A/ja

1987-03-03 Publication of JPS6248398A publication Critical patent/JPS6248398A/ja

1993-07-05 Publication of JPH0544280B2 publication Critical patent/JPH0544280B2/ja

Status Granted legal-status Critical Current

Links

102000004190 Enzymes Human genes 0.000 title claims abstract description 26
108090000790 Enzymes Proteins 0.000 title claims abstract description 26
238000000034 method Methods 0.000 title claims description 22
239000000126 substance Substances 0.000 claims abstract description 23
MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 claims abstract description 22
238000006243 chemical reaction Methods 0.000 claims abstract description 18
-1 4-methylumbelliferyl galactoside Chemical class 0.000 claims abstract description 10
150000001875 compounds Chemical class 0.000 claims abstract description 4
MUBZPKHOEPUJKR-UHFFFAOYSA-N Oxalic acid Natural products OC(=O)C(O)=O MUBZPKHOEPUJKR-UHFFFAOYSA-N 0.000 claims description 18
235000006408 oxalic acid Nutrition 0.000 claims description 8
238000004445 quantitative analysis Methods 0.000 claims description 3
CBZOGAWUNMFXFQ-UHFFFAOYSA-N bis(2,4-dinitrophenyl) oxalate Chemical compound [O-][N+](=O)C1=CC([N+](=O)[O-])=CC=C1OC(=O)C(=O)OC1=CC=C([N+]([O-])=O)C=C1[N+]([O-])=O CBZOGAWUNMFXFQ-UHFFFAOYSA-N 0.000 claims description 2
GEVPIWPYWJZSPR-UHFFFAOYSA-N tcpo Chemical group ClC1=CC(Cl)=CC(Cl)=C1OC(=O)C(=O)OC1=C(Cl)C=C(Cl)C=C1Cl GEVPIWPYWJZSPR-UHFFFAOYSA-N 0.000 claims 1
230000035945 sensitivity Effects 0.000 abstract description 7
239000003960 organic solvent Substances 0.000 abstract description 2
150000005690 diesters Chemical class 0.000 abstract 2
239000003054 catalyst Substances 0.000 abstract 1
230000002255 enzymatic effect Effects 0.000 abstract 1
229940088598 enzyme Drugs 0.000 description 19
239000003153 chemical reaction reagent Substances 0.000 description 18
238000004020 luminiscence type Methods 0.000 description 15
239000000758 substrate Substances 0.000 description 13
238000004458 analytical method Methods 0.000 description 10
239000000523 sample Substances 0.000 description 8
WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 description 6
XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 description 6
238000002795 fluorescence method Methods 0.000 description 6
238000005259 measurement Methods 0.000 description 6
239000000243 solution Substances 0.000 description 6
102000003914 Cholinesterases Human genes 0.000 description 5
108090000322 Cholinesterases Proteins 0.000 description 5
238000010521 absorption reaction Methods 0.000 description 5
229940048961 cholinesterase Drugs 0.000 description 5
238000002360 preparation method Methods 0.000 description 4
102000004882 Lipase Human genes 0.000 description 3
108090001060 Lipase Proteins 0.000 description 3
239000004367 Lipase Substances 0.000 description 3
239000000872 buffer Substances 0.000 description 3
230000000694 effects Effects 0.000 description 3
230000003301 hydrolyzing effect Effects 0.000 description 3
235000019421 lipase Nutrition 0.000 description 3
238000011002 quantification Methods 0.000 description 3
239000012488 sample solution Substances 0.000 description 3
239000011550 stock solution Substances 0.000 description 3
HSHNITRMYYLLCV-UHFFFAOYSA-N 4-methylumbelliferone Chemical compound C1=C(O)C=CC2=C1OC(=O)C=C2C HSHNITRMYYLLCV-UHFFFAOYSA-N 0.000 description 2
CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 2
CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
239000007864 aqueous solution Substances 0.000 description 2
ZYGHJZDHTFUPRJ-UHFFFAOYSA-N coumarin Chemical compound C1=CC=C2OC(=O)C=CC2=C1 ZYGHJZDHTFUPRJ-UHFFFAOYSA-N 0.000 description 2
238000001952 enzyme assay Methods 0.000 description 2
YQGOJNYOYNNSMM-UHFFFAOYSA-N eosin Chemical compound [Na+].OC(=O)C1=CC=CC=C1C1=C2C=C(Br)C(=O)C(Br)=C2OC2=C(Br)C(O)=C(Br)C=C21 YQGOJNYOYNNSMM-UHFFFAOYSA-N 0.000 description 2
150000002148 esters Chemical class 0.000 description 2
QRMZSPFSDQBLIX-UHFFFAOYSA-N homovanillic acid Chemical compound COC1=CC(CC(O)=O)=CC=C1O QRMZSPFSDQBLIX-UHFFFAOYSA-N 0.000 description 2
239000008363 phosphate buffer Substances 0.000 description 2
HSSLDCABUXLXKM-UHFFFAOYSA-N resorufin Chemical compound C1=CC(=O)C=C2OC3=CC(O)=CC=C3N=C21 HSSLDCABUXLXKM-UHFFFAOYSA-N 0.000 description 2
RGJTZDRUTUWWTD-UHFFFAOYSA-N (7-oxophenoxazin-3-yl) butanoate Chemical compound C1=CC(=O)C=C2OC3=CC(OC(=O)CCC)=CC=C3N=C21 RGJTZDRUTUWWTD-UHFFFAOYSA-N 0.000 description 1
FOSXAUKTMJXZDC-UHFFFAOYSA-N 2-oxo-2-(2,3,4-trichlorophenoxy)acetic acid Chemical compound OC(=O)C(=O)OC1=CC=C(Cl)C(Cl)=C1Cl FOSXAUKTMJXZDC-UHFFFAOYSA-N 0.000 description 1
241000589516 Pseudomonas Species 0.000 description 1
PLXBWHJQWKZRKG-UHFFFAOYSA-N Resazurin Chemical compound C1=CC(=O)C=C2OC3=CC(O)=CC=C3[N+]([O-])=C21 PLXBWHJQWKZRKG-UHFFFAOYSA-N 0.000 description 1
150000001413 amino acids Chemical class 0.000 description 1
239000000538 analytical sample Substances 0.000 description 1
238000000149 argon plasma sintering Methods 0.000 description 1
NWCHELUCVWSRRS-UHFFFAOYSA-N atrolactic acid Chemical compound OC(=O)C(O)(C)C1=CC=CC=C1 NWCHELUCVWSRRS-UHFFFAOYSA-N 0.000 description 1
238000011088 calibration curve Methods 0.000 description 1
229910002092 carbon dioxide Inorganic materials 0.000 description 1
239000001569 carbon dioxide Substances 0.000 description 1
239000007795 chemical reaction product Substances 0.000 description 1
238000007796 conventional method Methods 0.000 description 1
229960000956 coumarin Drugs 0.000 description 1
235000001671 coumarin Nutrition 0.000 description 1
238000001514 detection method Methods 0.000 description 1
239000012895 dilution Substances 0.000 description 1
238000010790 dilution Methods 0.000 description 1
MOTZDAYCYVMXPC-UHFFFAOYSA-N dodecyl hydrogen sulfate Chemical compound CCCCCCCCCCCCOS(O)(=O)=O MOTZDAYCYVMXPC-UHFFFAOYSA-N 0.000 description 1
229940043264 dodecyl sulfate Drugs 0.000 description 1
238000006911 enzymatic reaction Methods 0.000 description 1
230000005284 excitation Effects 0.000 description 1
150000008195 galaktosides Chemical class 0.000 description 1
239000007789 gas Substances 0.000 description 1
238000010438 heat treatment Methods 0.000 description 1
238000006460 hydrolysis reaction Methods 0.000 description 1
OSZNNLWOYWAHSS-UHFFFAOYSA-M neostigmine methyl sulfate Chemical compound COS([O-])(=O)=O.CN(C)C(=O)OC1=CC=CC([N+](C)(C)C)=C1 OSZNNLWOYWAHSS-UHFFFAOYSA-M 0.000 description 1
229960002253 neostigmine methylsulfate Drugs 0.000 description 1
BOPGDPNILDQYTO-NNYOXOHSSA-N nicotinamide-adenine dinucleotide Chemical compound C1=CCC(C(=O)N)=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]2[C@H]([C@@H](O)[C@@H](O2)N2C3=NC=NC(N)=C3N=C2)O)O1 BOPGDPNILDQYTO-NNYOXOHSSA-N 0.000 description 1
229930027945 nicotinamide-adenine dinucleotide Natural products 0.000 description 1
150000002942 palmitic acid derivatives Chemical class 0.000 description 1
125000001997 phenyl group Chemical class [H]C1=C([H])C([H])=C(*)C([H])=C1[H] 0.000 description 1
229920001184 polypeptide Polymers 0.000 description 1
102000004196 processed proteins & peptides Human genes 0.000 description 1
108090000765 processed proteins & peptides Proteins 0.000 description 1
239000011541 reaction mixture Substances 0.000 description 1
210000002966 serum Anatomy 0.000 description 1
239000012086 standard solution Substances 0.000 description 1
239000012089 stop solution Substances 0.000 description 1
238000012546 transfer Methods 0.000 description 1
UFTFJSFQGQCHQW-UHFFFAOYSA-N triformin Chemical compound O=COCC(OC=O)COC=O UFTFJSFQGQCHQW-UHFFFAOYSA-N 0.000 description 1
239000002023 wood Substances 0.000 description 1

Landscapes

Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

JP18581785A 1985-08-26 1985-08-26 化学発光法による酵素の定量法 Granted JPS6248398A (ja)

Priority Applications (1)

Application Number	Priority Date	Filing Date	Title
JP18581785A JPS6248398A (ja)	1985-08-26	1985-08-26	化学発光法による酵素の定量法

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
JP18581785A JPS6248398A (ja)	1985-08-26	1985-08-26	化学発光法による酵素の定量法

Publications (2)

Publication Number	Publication Date
JPS6248398A true JPS6248398A (ja)	1987-03-03
JPH0544280B2 JPH0544280B2 (fr)	1993-07-05

Family

ID=16177399

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
JP18581785A Granted JPS6248398A (ja)	1985-08-26	1985-08-26	化学発光法による酵素の定量法

Country Status (1)

Country	Link
JP (1)	JPS6248398A (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US5736320A (en) *	1993-05-12	1998-04-07	Schlederer; Thomas	Method of detecting substances by chemiluminescence

1985
- 1985-08-26 JP JP18581785A patent/JPS6248398A/ja active Granted

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US5736320A (en) *	1993-05-12	1998-04-07	Schlederer; Thomas	Method of detecting substances by chemiluminescence

Also Published As

Publication number	Publication date
JPH0544280B2 (fr)	1993-07-05

Publication	Publication Date	Title
Kurfürst et al.	1984	Characterization and postulated structure of the primary emitter in the bacterial luciferase reaction
Lu et al.	2006	Reactive oxygen species and their chemiluminescence-detection methods
US4743561A (en)	1988-05-10	Luminescent assay with a reagent to alter transmitive properties of assay solution
JP4274291B2 (ja)	2009-06-03	マルチレポータ遺伝子検定
Paul	1978	Recent analytical developments using chemiluminescence in solution
JPH0354304B2 (fr)	1991-08-19
DE69028591D1 (de)	1996-10-24	Haloperoxidase-Säure-Optimum Chemilumineszenztestsystem
JPH09511581A (ja)	1997-11-18	化学アッセイ較正方法
JPH0630628B2 (ja)	1994-04-27	生細胞数及び活力の測定方法
EP1521843B1 (fr)	2007-09-05	Procede de detection chimioluminiscente
Tsuji et al.	1989	Chemiluminescent Enzyme Immunoassay A Review
WO2014116727A1 (fr)	2014-07-31	Dosage rapide en un temps pour la détection d'inhibiteurs d'enzymes
US6099760A (en)	2000-08-08	Hydrogen peroxide based ECL
JPS6248398A (ja)	1987-03-03	化学発光法による酵素の定量法
CN112179877A (zh)	2021-01-05	一种基于催化反应原位荧光检测无机焦磷酸酶的方法
EP0408463A1 (fr)	1991-01-16	Compositions chimioluminescentes, procédés chimioluminescents et leur utilisation dans des essais analytiques
JPS63219398A (ja)	1988-09-13	過酸化水素の定量法
JP2004514460A (ja)	2004-05-20	ルシフェラーゼ生物発光に関する光活性型インビトロアッセイ法
Sato et al.	1990	Determination of acetaldehyde by a chemiluminescence method
JP3005238B2 (ja)	2000-01-31	化学発光検定法
SU1041568A1 (ru)	1983-09-15	Реагент дл определени аденозин-5-трифосфата
JPH066070B2 (ja)	1994-01-26	化学発光法によるｎａｄｈ又はｎａｄｐｈの定量法
US20020132364A1 (en)	2002-09-19	Quant-screentm chemiluminescent assays
JP2837414B2 (ja)	1998-12-16	細胞内の物質測定方法
JPH0698030B2 (ja)	1994-12-07	Ｎａｄｐｈの化学発光法による定量法

JPS6248398A - 化学発光法による酵素の定量法 - Google Patents

Info

Links

Landscapes

Priority Applications (1)

Applications Claiming Priority (1)

Publications (2)

Family

ID=16177399

Family Applications (1)

Country Status (1)

Cited By (1)

Cited By (1)

Also Published As

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