JPS63102696A - ジヒドロ葉酸還元酵素―ロイシンエンケファリン融合タンパクおよびその製造方法 - Google Patents

ジヒドロ葉酸還元酵素―ロイシンエンケファリン融合タンパクおよびその製造方法

Info

Publication number
JPS63102696A
JPS63102696A JP61249260A JP24926086A JPS63102696A JP S63102696 A JPS63102696 A JP S63102696A JP 61249260 A JP61249260 A JP 61249260A JP 24926086 A JP24926086 A JP 24926086A JP S63102696 A JPS63102696 A JP S63102696A
Authority
JP
Japan
Prior art keywords
fusion protein
leucine enkephalin
dihydrofolate reductase
lek
dhfr
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP61249260A
Other languages
English (en)
Japanese (ja)
Other versions
JPH0364517B2 (2
Inventor
Masahiro Iwakura
正寛 巖倉
Tomokuni Kokubu
国分　友邦
Kiyotaka Furusawa
古澤　清孝
Shinichi Ohashi
信一 大箸
Keishiro Tsuda
津田　圭四郎
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
National Institute of Advanced Industrial Science and Technology AIST
Original Assignee
Agency of Industrial Science and Technology
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Agency of Industrial Science and Technology filed Critical Agency of Industrial Science and Technology
Priority to JP61249260A priority Critical patent/JPS63102696A/ja
Publication of JPS63102696A publication Critical patent/JPS63102696A/ja
Publication of JPH0364517B2 publication Critical patent/JPH0364517B2/ja
Granted legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0026—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on CH-NH groups of donors (1.5)
    • C12N9/0028—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on CH-NH groups of donors (1.5) with NAD or NADP as acceptor (1.5.1)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
JP61249260A 1986-10-20 1986-10-20 ジヒドロ葉酸還元酵素―ロイシンエンケファリン融合タンパクおよびその製造方法 Granted JPS63102696A (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP61249260A JPS63102696A (ja) 1986-10-20 1986-10-20 ジヒドロ葉酸還元酵素―ロイシンエンケファリン融合タンパクおよびその製造方法

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP61249260A JPS63102696A (ja) 1986-10-20 1986-10-20 ジヒドロ葉酸還元酵素―ロイシンエンケファリン融合タンパクおよびその製造方法

Publications (2)

Publication Number Publication Date
JPS63102696A true JPS63102696A (ja) 1988-05-07
JPH0364517B2 JPH0364517B2 (2) 1991-10-07

Family

ID=17190315

Family Applications (1)

Application Number Title Priority Date Filing Date
JP61249260A Granted JPS63102696A (ja) 1986-10-20 1986-10-20 ジヒドロ葉酸還元酵素―ロイシンエンケファリン融合タンパクおよびその製造方法

Country Status (1)

Country Link
JP (1) JPS63102696A (2)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH05339295A (ja) * 1991-12-05 1993-12-21 Agency Of Ind Science & Technol 抗体の作成方法
US6022953A (en) * 1989-04-19 2000-02-08 Chiron Corporation Multifunctional M-CSF proteins and genes encoding therefor

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6022953A (en) * 1989-04-19 2000-02-08 Chiron Corporation Multifunctional M-CSF proteins and genes encoding therefor
JPH05339295A (ja) * 1991-12-05 1993-12-21 Agency Of Ind Science & Technol 抗体の作成方法

Also Published As

Publication number Publication date
JPH0364517B2 (2) 1991-10-07

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Legal Events

Date Code Title Description
EXPY Cancellation because of completion of term