JPS63141907A - Cosmetic compounded with immobilized enzyme - Google Patents

Abstract

PURPOSE:To obtain a cosmetic safe to human body, by immobilizing an enzyme capable of decomposing saccharides, proteins and lipids causing the stain of skin, i.e. amylase, protease and lipase on a water-insoluble carrier and compounding the immobilized enzyme. CONSTITUTION:At least one kind of enzyme selected from amylase decomposing saccharides and glycogen (e.g. alpha-amylase), protease decomposing proteins (e.g. pepsin, papain, etc.) and lipase decomposing lipids (e.g. triacetin) is immobilized on an insoluble carrier such as inorganic carrier (e.g. titanium oxide, kaolinite, China clay, etc.) or an organic carrier (e.g. ion exchange resin, polyamino acid, cellulose, etc.) using a binder such as cyanogen bromide, glutaraldehyde, etc., and the immobilized enzyme is compounded to a cosmetic. Same effect can be attained by using microcapsules including the enzyme instead of immobilized enzyme.

Description

Detailed Description of the Invention (Field of Industrial Application) The present invention relates to cosmetics, and more particularly to cosmetics containing at least one immobilized enzyme among amylase, protease, and lipase immobilized on an insoluble carrier. It is something.

(Prior Art) The skin constantly secretes sebum from the sebaceous glands and sweat from the sweat glands, and renews the skin's dead skin through metabolism.

There are individual differences in the amount of sebum and sweat secreted and the amount of dead skin cells renewed. Excessive sebum secretion exhibits a so-called oily phenomenon, which not only makes cosmetics difficult to apply, but also tends to cause seborrhea, acne, and the like. Further, the hardening of skin keratin by ultraviolet rays causes the skin to lose its elasticity and reduce the water retention capacity of the skin. In this way, skin function requires that a proper skin condition be maintained at all times. Various cosmetics are used to keep the skin normal, to perform its functions smoothly, and to give it an aesthetic appearance. For example, an astringent lotion is used to suppress excess oil on the skin and astringent the skin, an alkaline lotion is used to wash away dirt and secreted oil, and a vanishing lotion is used to moisturize the skin and prevent roughness. Creams or emulsions are used for hardening of skin keratin due to poor sebum secretion, sunburn, etc. In this way, various chemicals are added to various cosmetics and used to remove sebum and treat hardened keratin. That is, keratolytic agents such as salicylic acid, sulfur, and urea are added to cosmetics to remove dead skin, and soap and alkaline substances are added to remove sebum. The cause of skin stains is secreted fat, protein, and carbohydrates.
The idea of using enzymes that break down these substances in cosmetics has existed since ancient times, but enzymes are used in various drugs, p++
It is unstable to temperature and other factors, so if it is mixed into cosmetics as it is, it will quickly lose its activity, and it will lose its activity during storage or manufacturing, making it difficult to expect its effects, so only a limited number of products are used. It wasn't.

(Problems to be Solved by the Invention) The present invention provides cosmetics by immobilizing enzymes amylase, protease, and lipase that decompose substances, proteins, and lipids that cause skin stains on a water-insoluble carrier. Also, instead of immobilizing these enzymes in an insoluble carrier, the enzymes are encapsulated in capsules and incorporated into cosmetics, which allows any commercially available enzyme to be incorporated into cosmetics, and can be used over a wide temperature and pH range. By providing enzyme-containing cosmetics that are stable over a wide range and can be stored for a long time,
This product provides cosmetics that are safe for the human body without the use of keratolytic agents, alkalis, or other chemicals conventionally used to remove sebum and exfoliation.

(Means for solving the problem) The enzyme amylase that decomposes carbohydrates and glycogen includes α-amylase, β-amylase, and saccharified amylase.
Pig pancreas, barley germ, sweet potato, barley, bacillus,
Enzymes extracted from Aspergillus bacteria are commercially available, and enzymes that decompose proteins include animal-based enzymes such as pepsin, trypsin, chymotrypsin, and kallikren, plant-based enzymes such as papain, promelain, and ficin, Streptomyces, Bacterial proteases such as molds are commercially available, and lipases that decompose lipids include animal-based proteases such as triacetin, tristearin, and triolein, plant-based proteases such as extracts from wheat germ, castor seeds, and nata seeds, and lipases that break down lipids. Fungal and bacterial lipases such as extracts from Candida and Arachnidium are commercially available, and any of these commercially available enzymes can be used in the present invention. Insoluble carriers include inorganic materials such as titanium oxide, kaolinite, clay, calcium phosphate, zinc oxide, hydroxyapatite, and zeolite, ion exchange resins, polyamino acids, carboxymethyl cellulose, p-aminobenzene cellulose, cellulose, and granular silicon aminosilane derivatives. Organic substances can be used, but the size of the carrier particles is 9
It is desirable that the thickness is greater than or equal to μ and less than or equal to 50 μ. This is because fine particles of 9 μm or less are penetrated through the skin, and particles of 50 μm or more give a rough appearance to the skin. Binding agents for immobilizing enzymes include cyanogen bromide, glutaraldehyde, N
, N'-ethylene bismaleimide, hexamethylene diamine, hexamethylene diisocyanate, bisdiazobenzidine, etc. are used. The enzyme, insoluble carrier, and binder to be used can be arbitrarily selected depending on the type of cosmetic product to be used, and their immobilization can be performed by existing immobilization methods. That is, the enzyme, binder, and insoluble carrier are
Add to a buffer solution near H7 and stir at low temperature (about 5°C) for several hours to obtain a composite. The obtained insoluble enzyme is added to a buffer solution,
It is then thoroughly washed with cold water and dried by lyophilization.

The same effect can be obtained by using microcapsules containing enzymes instead of enzyme immobilization. Microencapsulation of enzymes can also be performed using existing methods. That is, an enzyme solution is added to an organic solution such as benzene or ether containing nylon, polystyrene, polyisocyanate, etc. to completely emulsify it, and this emulsion is dispersed in a protective colloid solution containing a nonionic surfactant or gelatin. It depends on the situation.

The immobilized enzymes or microcapsules obtained in this way have long-term enzymatic activity and are safe for the human body when incorporated into lotions, creams, lotions, and other cosmetic raw materials according to the purpose. It is possible to provide cosmetics that safely and efficiently decompose keratin, lipids, and carbohydrates and promote skin metabolism.

(Action) Because the enzyme is immobilized or microencapsulated, it exhibits stable enzymatic activity over a wide range of temperature and pl+, and the immobilization increases the Michaeli constant, resulting in a faster decomposition rate. It breaks down dead skin cells in a short time and is safe for the human body.

The present invention will be specifically explained below with reference to Examples.

The enzymes used in this experiment were proteolytic biobrase, wheat germ lipase, and barley amylase, and their immobilization was performed as follows. Finely divided hydroxyapatite was suspended in an ice-cooled buffer solution with a pH of 7.5, and the same buffer solution containing xamethylenediamine was added to the mixture under stirring.After a few minutes, an enzyme dissolved in the same buffer solution was added, and the mixture was incubated at 4°C. After stirring overnight, the solid obtained by centrifugation was thoroughly washed with a buffer solution and cold water, and lyophilized. In each experiment, the immobilized enzyme or an aqueous solution in which the immobilized enzyme was suspended was added to the resulting dispersion in which components other than the immobilized enzyme were added to the surfactant heated to 50°C with vigorous stirring, and the mixture was stirred. While cooling to room temperature.

Example 1. Oil-based ointment type foundation Weight% Immobilized protease 10 Isopropyl valmitate 15 Lanolin alcohol
5 acetic acid lanolin
8 micro crystal wax
7 Liquid paraffin 25 Candelilla wax 0.5 Powdered hydroxyapatite 3゜Preservative, antioxidant
Appropriate amount example 2. Oil-based stick type foundation Weight% Immobilized lipase, immobilized protease, immobilized amylase 15 Zinc white
3゜Liquid paraffin
18 Isopropyl myristate 15
Liquid Lanolin 4.5 Microcrystalline Wax 4.5 Ceresin
10 carnauba wax
2 Sorbitan sesquioleate
Preservatives, antioxidants Appropriate amount Flavoring Example 3. Emulsion foundation weight % Immobilized protease, immobilized lipase 12 Stearic acid 2.4 Propylene glycol monostearate 2 Cetostearyl alcohol 0.2 Liquid lanolin
2 liquid paraffin
3 Isopropyl myristate 8.5 Adjust to 100 with water.

(Effects of the invention) Since the enzyme is immobilized, it exhibits enzyme activity over a wide temperature and pH range, shortens the enzyme's action time, effectively removes impurities from the skin, and hides the insoluble carrier itself. The concealing power of the cosmetics itself can be seen to improve.

Claims (1)

[Claims] A cosmetic containing an immobilized enzyme characterized by containing at least one of amylase, protease, and lipase immobilized on an insoluble carrier.