JPS6320031A - Chemical reaction device - Google Patents

Abstract

PURPOSE:To easily set a reacting condition and to improve the yield by constituting a chemical reaction device with a floating body which keeps a chemical reaction material sticking and a tapered pipe the inner diameter of which are smaller at its lower end and larger at its top end than the outer diameter of the floating body. CONSTITUTION:The protein in a starting material is adsorbed on the surface of the floating body 2 made of glass, but the starting material sticking to the inner wall of the tapered pipe 1 made of 'Teflon(R)', falls down in the shape of water drops with the protein contained in said material. The fallen material is stored in the lower of a neplizer 9 and recovered to enable to be used again by injecting from a liquid injecting port 7. When the high temp. mixed gas of trifluoroacetic acid, hydrogen chloride, and water vapor are injected from a gas injecting port 6, the protein sticking to the surface of the floating body 2 is hydrolyzed. The, the mixed gas still existent is removed by the gaseous nitrgen injected from the gas injecting port 6, and the hydrolysis product of the protein on the surface of the floating body 2 is dissolved in the water injected from the liquid injecting port 7.

Description

DETAILED DESCRIPTION OF THE INVENTION [Field of Industrial Application] The present invention relates to a chemical reaction apparatus used for chemical reactions such as trace amounts of chemical synthesis or chemical analysis.

[Conventional technology]

Conventionally, in chemical reaction devices used for chemical reactions such as small amounts of chemical synthesis or chemical analysis, the parts that fix and hold chemical reactants have used columns filled with fine particles or rotating cups. .

For example, a commercially available DNA synthesizer is an example of the former, and a commercially available peptide analyzer is an example of the latter.

[Problem that the invention seeks to solve]

Conventional column-type chemical reaction devices are excellent for performing small amounts of chemical reactions in the liquid phase, but the liquid that gets between the particles permeates through the capillary phenomenon, so it is difficult to remove the liquid. << Conversely, when a liquid was injected into a column that had been dried once, bubbles were generated, and in the areas where the bubbles existed, the reaction did not proceed or impurities were generated due to side reactions.

On the other hand, in the method using a rotary force 7, a small amount of the chemically reacting material is concentrated above the cup, and a large amount of the chemically reacting material is concentrated below the cup due to gravity, especially when the reactant is a polymeric substance. This had the disadvantage that the reaction rate was different between the upper and lower parts of the cup, making it difficult to set the reaction time and reducing the yield.

[Means for solving problems]

In order to solve the above-mentioned problems, the present invention installs a tapered pipe with the thick end on top and the thin end on the bottom, and inside the tapered pipe, the inside diameter is larger than the lower end of the tapered pipe, and the upper end of the tapered pipe has a larger inner diameter than the lower end of the tapered pipe. By fixing and retaining the chemical reactant on the surface of the floating body, which has an outer diameter smaller than the inner diameter, a homogeneous film is formed, and a chemical reaction occurs with the reactant passed through the tapered tube, resulting in a gas phase and a liquid phase. The purpose is to provide a chemical reaction device that can control any of the following reactions.

[Effect]

FIG. 1 is a sectional view showing the operation of the chemical reaction apparatus according to the present invention, in which a starting sample supplied from a supply port 3 is fixed and retained on a floating body 2 inserted into a tapered tube 1. There are two ways to supply the starting sample: one is to supply a liquid in which the starting sample is dissolved or suspended in a liquid, and the other is to supply the starting sample to a liquid in which the starting sample is dissolved or suspended.
The dissolved or suspended gas may be supplied as a gas or a mist to absorb or adsorb chemically reactive substances in the starting sample.
After being fixed and held on the surface of the floating body through chemical bonding,
A cleaning gas or liquid is supplied from the supply port, excess starting samples other than the starting samples fixed and held on the surface of the floating body are discharged into the tapered tube, and reactants and cleaning agents are sequentially added to the gas, mist, or Alternatively, by supplying it as a liquid, a predetermined chemical reaction can proceed.

〔Example〕

FIG. 2 is a sectional view showing an embodiment of the chemical reaction apparatus according to the present invention, in which a glass spiral floating body is placed in a Teflon tapered tube, and a cap with a discharge port is covered. This directs the waste to the waste tank. On the other hand, a nebulizer 9 equipped with a liquid injection lower, a gas injection port 6, and a liquid drain port 8 is connected to the lower end of the tapered tube, and the liquid drain port 8 is equipped with a stopper so as to be opened only when draining the liquid. There is.

For example, when performing protein hydrolysis using the chemical reaction apparatus described above, first inject an aqueous solution of protein, which is a starting sample, from the liquid injection port, and at the same time inject nitrogen gas from the gas injection port. The sample becomes atomized and rises inside the tapered tube, adhering to the inner wall of the tapered tube and the surface of the floating body.The floating body is pushed up by the gas pressure and rotates at the same time, but it does not touch the surface of the glass floating body. The protein in the starting sample is adsorbed, but the starting sample adhering to the inner wall of the tapered Teflon tube falls as water droplets along with the proteins contained inside, collects at the bottom of the nebulizer, and is collected and injected again from the liquid inlet. Second, when a high-temperature mixed gas of trifluoroacetic acid, hydrogen chloride, and water vapor is injected from the gas inlet, the proteins attached to the surface of the floating body are hydrolyzed, and then the proteins attached to the surface of the floating body are hydrolyzed. The remaining mixed gas is removed by the injected nitrogen gas, and finally, the protein hydrolyzate on the surface of the floating body is dissolved in the water injected from the liquid injection port, and can be taken out from the drain port. ] According to the present invention, since a uniform reaction can be carried out,
It is possible to easily set reaction conditions and to obtain a high yield, which is a remarkable effect.

[Brief explanation of the drawing]

FIG. 1 is a sectional view showing the operation of a chemical reaction apparatus according to the present invention, and FIG. 2 is a sectional view showing an embodiment of the present invention. 1... Tapered tube 2... Floating body 3... Above the supply port Figure 1 Figure 2

Claims (1)

[Claims] 1) A floating body for fixing and holding a chemically reactive substance, and a tapered tube whose inner diameter at the lower end is thinner than the outer diameter of the floating body and whose inner diameter at the upper end is thicker than the front floating body. Chemical reactor. 2) The chemical reaction device according to claim 1, characterized in that a nebulizer is connected to the lower end of the tapered tube. 3) The chemical reaction apparatus according to claims 1 and 2, further comprising means for supplying and discharging liquid or gas to the upper end or lower end of the tapered tube. 4) The chemical reaction device according to Claims 1, 2, and 3, wherein the floating body has a spiral shape.