JPS63248321A - 組織培養による薬用人参の大量生産方法 - Google Patents
組織培養による薬用人参の大量生産方法Info
- Publication number
- JPS63248321A JPS63248321A JP62083641A JP8364187A JPS63248321A JP S63248321 A JPS63248321 A JP S63248321A JP 62083641 A JP62083641 A JP 62083641A JP 8364187 A JP8364187 A JP 8364187A JP S63248321 A JPS63248321 A JP S63248321A
- Authority
- JP
- Japan
- Prior art keywords
- medium
- ginseng
- acid
- plant growth
- tissue culture
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000004519 manufacturing process Methods 0.000 title claims description 14
- 244000000626 Daucus carota Species 0.000 title claims description 5
- 235000002767 Daucus carota Nutrition 0.000 title claims description 5
- 240000004371 Panax ginseng Species 0.000 claims description 86
- 235000008434 ginseng Nutrition 0.000 claims description 75
- 235000003140 Panax quinquefolius Nutrition 0.000 claims description 47
- 206010020649 Hyperkeratosis Diseases 0.000 claims description 46
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 claims description 44
- 239000005648 plant growth regulator Substances 0.000 claims description 37
- 230000000392 somatic effect Effects 0.000 claims description 37
- SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 claims description 32
- 235000002789 Panax ginseng Nutrition 0.000 claims description 31
- 241000196324 Embryophyta Species 0.000 claims description 26
- 210000002257 embryonic structure Anatomy 0.000 claims description 23
- 238000000034 method Methods 0.000 claims description 21
- 239000007787 solid Substances 0.000 claims description 20
- NWBJYWHLCVSVIJ-UHFFFAOYSA-N N-benzyladenine Chemical compound N=1C=NC=2NC=NC=2C=1NCC1=CC=CC=C1 NWBJYWHLCVSVIJ-UHFFFAOYSA-N 0.000 claims description 19
- PRPINYUDVPFIRX-UHFFFAOYSA-N 1-naphthaleneacetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CC=CC2=C1 PRPINYUDVPFIRX-UHFFFAOYSA-N 0.000 claims description 17
- 230000004069 differentiation Effects 0.000 claims description 17
- JTEDVYBZBROSJT-UHFFFAOYSA-N indole-3-butyric acid Chemical compound C1=CC=C2C(CCCC(=O)O)=CNC2=C1 JTEDVYBZBROSJT-UHFFFAOYSA-N 0.000 claims description 17
- 230000006698 induction Effects 0.000 claims description 17
- 239000003617 indole-3-acetic acid Substances 0.000 claims description 16
- 210000001161 mammalian embryo Anatomy 0.000 claims description 15
- IXORZMNAPKEEDV-OBDJNFEBSA-N gibberellin A3 Chemical compound C([C@@]1(O)C(=C)C[C@@]2(C1)[C@H]1C(O)=O)C[C@H]2[C@]2(C=C[C@@H]3O)[C@H]1[C@]3(C)C(=O)O2 IXORZMNAPKEEDV-OBDJNFEBSA-N 0.000 claims description 12
- 239000005980 Gibberellic acid Substances 0.000 claims description 11
- 238000012258 culturing Methods 0.000 claims description 11
- IXORZMNAPKEEDV-UHFFFAOYSA-N gibberellic acid GA3 Natural products OC(=O)C1C2(C3)CC(=C)C3(O)CCC2C2(C=CC3O)C1C3(C)C(=O)O2 IXORZMNAPKEEDV-UHFFFAOYSA-N 0.000 claims description 11
- 229910017053 inorganic salt Inorganic materials 0.000 claims description 8
- 230000015572 biosynthetic process Effects 0.000 claims description 6
- 235000002791 Panax Nutrition 0.000 claims description 4
- 241000208343 Panax Species 0.000 claims description 4
- 239000000203 mixture Substances 0.000 claims description 4
- 230000001172 regenerating effect Effects 0.000 claims description 4
- 241000511582 Actinomyces meyeri Species 0.000 claims description 3
- 240000007190 Daucus pusillus Species 0.000 claims description 3
- 235000002196 Daucus pusillus Nutrition 0.000 claims description 3
- 241000180649 Panax notoginseng Species 0.000 claims description 2
- 235000003143 Panax notoginseng Nutrition 0.000 claims description 2
- 230000001939 inductive effect Effects 0.000 claims description 2
- 150000001875 compounds Chemical class 0.000 claims 3
- OVSKIKFHRZPJSS-DOMIDYPGSA-N 2-(2,4-dichlorophenoxy)acetic acid Chemical compound OC(=O)[14CH2]OC1=CC=C(Cl)C=C1Cl OVSKIKFHRZPJSS-DOMIDYPGSA-N 0.000 claims 2
- 241001530105 Anax Species 0.000 claims 1
- 241000784863 Stomatium Species 0.000 claims 1
- 239000010907 stover Substances 0.000 claims 1
- 239000002609 medium Substances 0.000 description 81
- 239000006870 ms-medium Substances 0.000 description 11
- FAIXYKHYOGVFKA-UHFFFAOYSA-N Kinetin Natural products N=1C=NC=2N=CNC=2C=1N(C)C1=CC=CO1 FAIXYKHYOGVFKA-UHFFFAOYSA-N 0.000 description 8
- QANMHLXAZMSUEX-UHFFFAOYSA-N kinetin Chemical compound N=1C=NC=2N=CNC=2C=1NCC1=CC=CO1 QANMHLXAZMSUEX-UHFFFAOYSA-N 0.000 description 8
- 229960001669 kinetin Drugs 0.000 description 8
- 239000007788 liquid Substances 0.000 description 4
- 240000005373 Panax quinquefolius Species 0.000 description 3
- 239000004480 active ingredient Substances 0.000 description 3
- 210000004748 cultured cell Anatomy 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 239000001963 growth medium Substances 0.000 description 3
- 229930000044 secondary metabolite Natural products 0.000 description 3
- 239000002689 soil Substances 0.000 description 3
- UZKQTCBAMSWPJD-UQCOIBPSSA-N trans-Zeatin Natural products OCC(/C)=C\CNC1=NC=NC2=C1N=CN2 UZKQTCBAMSWPJD-UQCOIBPSSA-N 0.000 description 3
- UZKQTCBAMSWPJD-FARCUNLSSA-N trans-zeatin Chemical compound OCC(/C)=C/CNC1=NC=NC2=C1N=CN2 UZKQTCBAMSWPJD-FARCUNLSSA-N 0.000 description 3
- 229940023877 zeatin Drugs 0.000 description 3
- 229920001817 Agar Polymers 0.000 description 2
- 240000002769 Morchella esculenta Species 0.000 description 2
- 235000002779 Morchella esculenta Nutrition 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- UQHKFADEQIVWID-UHFFFAOYSA-N cytokinin Natural products C1=NC=2C(NCC=C(CO)C)=NC=NC=2N1C1CC(O)C(CO)O1 UQHKFADEQIVWID-UHFFFAOYSA-N 0.000 description 2
- 239000004062 cytokinin Substances 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- -1 etc. are suitable Substances 0.000 description 2
- 239000012467 final product Substances 0.000 description 2
- 230000008635 plant growth Effects 0.000 description 2
- 239000003375 plant hormone Substances 0.000 description 2
- 230000035755 proliferation Effects 0.000 description 2
- 230000001902 propagating effect Effects 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- 238000009331 sowing Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- COVMVPHACFXMAX-OYNOKLRGSA-N (-)-morellic acid Chemical compound C1=CC(C)(C)OC2=C1C(O)=C1C(=O)C3=C[C@@H](C(=O)[C@]4(C\C=C(\C)C(O)=O)OC5(C)C)C[C@@H]5[C@]34OC1=C2CC=C(C)C COVMVPHACFXMAX-OYNOKLRGSA-N 0.000 description 1
- 239000005631 2,4-Dichlorophenoxyacetic acid Substances 0.000 description 1
- 229930192334 Auxin Natural products 0.000 description 1
- 244000025254 Cannabis sativa Species 0.000 description 1
- XNIZIBPYBUCVEU-UHFFFAOYSA-N Morellic acid Natural products CC1Oc2c(CC=C(C)C)c3OC45C6CC(C=C4C(=O)c3c(O)c2C=C1)C(=O)C5(CC=C(C)/C(=O)O)OC6(C)C XNIZIBPYBUCVEU-UHFFFAOYSA-N 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 239000002363 auxin Substances 0.000 description 1
- 210000003323 beak Anatomy 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000012790 confirmation Methods 0.000 description 1
- 239000007799 cork Substances 0.000 description 1
- 238000012364 cultivation method Methods 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 229940107131 ginseng root Drugs 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- COVMVPHACFXMAX-ZVKSWBPMSA-N isomorellic acid Natural products O=C(O)/C(=C\C[C@]12C(=O)[C@H]3C=C4C(=O)c5c(O)c6c(c(C/C=C(\C)/C)c5O[C@]14[C@@H](C(C)(C)O2)C3)OC(C)(C)C=C6)/C COVMVPHACFXMAX-ZVKSWBPMSA-N 0.000 description 1
- HRRDCWDFRIJIQZ-UHFFFAOYSA-N naphthalene-1,8-dicarboxylic acid Chemical compound C1=CC(C(O)=O)=C2C(C(=O)O)=CC=CC2=C1 HRRDCWDFRIJIQZ-UHFFFAOYSA-N 0.000 description 1
- 238000011017 operating method Methods 0.000 description 1
- 230000002062 proliferating effect Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 229930182490 saponin Natural products 0.000 description 1
- 150000007949 saponins Chemical class 0.000 description 1
- 235000017709 saponins Nutrition 0.000 description 1
- 230000009105 vegetative growth Effects 0.000 description 1
Landscapes
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP62083641A JPS63248321A (ja) | 1987-04-03 | 1987-04-03 | 組織培養による薬用人参の大量生産方法 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP62083641A JPS63248321A (ja) | 1987-04-03 | 1987-04-03 | 組織培養による薬用人参の大量生産方法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS63248321A true JPS63248321A (ja) | 1988-10-14 |
| JPH0430809B2 JPH0430809B2 (de) | 1992-05-22 |
Family
ID=13808075
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP62083641A Granted JPS63248321A (ja) | 1987-04-03 | 1987-04-03 | 組織培養による薬用人参の大量生産方法 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS63248321A (de) |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6416582A (en) * | 1987-07-11 | 1989-01-20 | Wakunaga Pharma Co Ltd | Culture and production of root-like tissue of plant of panax genus |
| CN102067849A (zh) * | 2011-02-11 | 2011-05-25 | 吉林农业大学 | 氯化胆碱、苄氨基嘌呤和吲哚丁酸混用促进人参根生长的方法 |
| CN104126394A (zh) * | 2014-07-25 | 2014-11-05 | 普定县银丰农业科技发展有限公司 | 一种三七的种植方法 |
| WO2017020769A1 (zh) * | 2015-08-05 | 2017-02-09 | 广西壮族自治区药用植物园 | 一种提高三七中三七皂苷r1含量的方法 |
| CN107409690A (zh) * | 2017-06-20 | 2017-12-01 | 合肥卓畅农业科技有限公司 | 一种三七的高产种植方法 |
| CN108029491A (zh) * | 2018-02-12 | 2018-05-15 | 丽江滇西本草药业有限公司 | 一种高海拔三七种植方法 |
| CN109964771A (zh) * | 2019-03-21 | 2019-07-05 | 文山高田三七中药饮片有限公司 | 一种三七的种植方法 |
| CN109964770A (zh) * | 2019-03-21 | 2019-07-05 | 文山高田三七中药饮片有限公司 | 一种改善三七连作障碍的种植方法 |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS62151117A (ja) * | 1985-08-29 | 1987-07-06 | 武田薬品工業株式会社 | オタネニンジンの再生植物作出方法 |
| JPS63133922A (ja) * | 1986-11-27 | 1988-06-06 | 日本鉱業株式会社 | オタネニンジンの組織培養方法 |
-
1987
- 1987-04-03 JP JP62083641A patent/JPS63248321A/ja active Granted
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS62151117A (ja) * | 1985-08-29 | 1987-07-06 | 武田薬品工業株式会社 | オタネニンジンの再生植物作出方法 |
| JPS63133922A (ja) * | 1986-11-27 | 1988-06-06 | 日本鉱業株式会社 | オタネニンジンの組織培養方法 |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6416582A (en) * | 1987-07-11 | 1989-01-20 | Wakunaga Pharma Co Ltd | Culture and production of root-like tissue of plant of panax genus |
| CN102067849A (zh) * | 2011-02-11 | 2011-05-25 | 吉林农业大学 | 氯化胆碱、苄氨基嘌呤和吲哚丁酸混用促进人参根生长的方法 |
| CN104126394A (zh) * | 2014-07-25 | 2014-11-05 | 普定县银丰农业科技发展有限公司 | 一种三七的种植方法 |
| WO2017020769A1 (zh) * | 2015-08-05 | 2017-02-09 | 广西壮族自治区药用植物园 | 一种提高三七中三七皂苷r1含量的方法 |
| CN107409690A (zh) * | 2017-06-20 | 2017-12-01 | 合肥卓畅农业科技有限公司 | 一种三七的高产种植方法 |
| CN108029491A (zh) * | 2018-02-12 | 2018-05-15 | 丽江滇西本草药业有限公司 | 一种高海拔三七种植方法 |
| CN109964771A (zh) * | 2019-03-21 | 2019-07-05 | 文山高田三七中药饮片有限公司 | 一种三七的种植方法 |
| CN109964770A (zh) * | 2019-03-21 | 2019-07-05 | 文山高田三七中药饮片有限公司 | 一种改善三七连作障碍的种植方法 |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0430809B2 (de) | 1992-05-22 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Skirvin et al. | In vitro propagation of ‘forerer yours’ rose1 | |
| Agrawal et al. | Effective protocol for in vitro shoot production through nodal explants of Simmondsia chinensis | |
| Barra-Jiménez et al. | Cloning mature holm oak trees by somatic embryogenesis | |
| Purkayastha et al. | Rapid in vitro multiplication and plant regeneration from nodal explants of Andrographis paniculata: a valuable medicinal plant | |
| Samosir et al. | Improving acclimatization through the photoautotrophic culture of coconut (Cocos nucifera) seedlings: an in vitro system for the efficient exchange of germplasm | |
| Chen et al. | High-frequency somatic embryogenesis from germinated zygotic embryos of Schisandra chinensis and evaluation of the effects of medium strength, sucrose, GA3, and BA on somatic embryo development | |
| Wen et al. | Efficient protocols for the micropropagation of tree peony (Paeonia suffruticosa ‘Jin Pao Hong’, P. suffruticosa ‘Wu Long Peng Sheng’, and P.× lemoinei ‘High Noon’) and application of arbuscular mycorrhizal fungi to improve plantlet establishment | |
| Benmahioul et al. | In vitro embryo germination and proliferation of pistachio (Pistacia vera L.) | |
| JPS63248321A (ja) | 組織培養による薬用人参の大量生産方法 | |
| Singh et al. | Somatic embryogenesis and plant regeneration from suspension cultures of Sapindus trifoliatus | |
| Sankar-Thomas et al. | Plant regeneration via somatic embryogenesis of Camptotheca acuminata in temporary immersion system (TIS) | |
| EP0317512B1 (de) | Eine leistungsfähige Methode, Baumwolle aus kultivierten Zellen zu regenerieren | |
| Yang et al. | Callus induction and high-efficiency plant regeneration via somatic embryogenesis in Papaver nudicaule L., an ornamental medicinal plant | |
| CN112021180A (zh) | 板栗体细胞胚发育的同步化方法及组培苗生根方法 | |
| Xu et al. | Efficient in vitro plant regeneration of Pinellia ternata (Thunb) Breit | |
| KR102602790B1 (ko) | 조직배양을 통한 일천궁의 대량 증식 방법 | |
| Ranjan Deb et al. | Callus mediated indirect somatic embryogenesis and plant regeneration of Saurauia punduana Wallich (Actinidiaceae) from in vitro cotyledonary leaves | |
| Ohki et al. | The effects of inorganic salts and growth regulators on in vitro shoot proliferation and leaf chlorophyll content of Delphinium cardinale | |
| Deb et al. | Rapid multiplication and induction of early in vitro flowering in Dendrobium primulinum Lindl | |
| JPH0463527A (ja) | クローン増殖による種苗の生産方法 | |
| CA1270647A (en) | Process for regenerating soybeans | |
| US10028505B2 (en) | Micropropagation of alexandrian laurel (Danae racemosa L) | |
| HISAJIMA et al. | Microplant propagation of rice plant in vitro | |
| Khanna et al. | Regeneration via organogenesis in callus cultures of Argyrolobium roseum | |
| Gopi et al. | In vitro plant regeneration through somatic embryogenesis in medicinally important leaf explants of Coleus forskohlii Briq |