JPS6324886A - 菌体または酵素の固定化方法 - Google Patents

菌体または酵素の固定化方法

Info

Publication number: JPS6324886A
Authority: JP; Japan
Prior art keywords: organic solvent; immobilized; mixed; enzymes; tube
Prior art date: 1986-07-18
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Granted

Application number

JP61170247A

Other languages

English (en)

Japanese (ja)

Other versions

JPH0560355B2 (2

Inventor

Nobuyuki Machida

町田　信幸

Jun Kimura

純木村

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

NEC Corp

Original Assignee

NEC Corp

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1986-07-18

Filing date

1986-07-18

Publication date

1988-02-02

1986-07-18 Application filed by NEC Corp filed Critical NEC Corp

1986-07-18 Priority to JP61170247A priority Critical patent/JPS6324886A/ja

1988-02-02 Publication of JPS6324886A publication Critical patent/JPS6324886A/ja

1993-09-02 Publication of JPH0560355B2 publication Critical patent/JPH0560355B2/ja

Status Granted legal-status Critical Current

Links

108090000790 Enzymes Proteins 0.000 title claims abstract description 19
102000004190 Enzymes Human genes 0.000 title claims abstract description 19
230000000813 microbial effect Effects 0.000 title abstract description 9
239000003960 organic solvent Substances 0.000 claims abstract description 45
230000001580 bacterial effect Effects 0.000 claims description 28
238000000034 method Methods 0.000 claims description 19
229920001477 hydrophilic polymer Polymers 0.000 claims description 14
239000011259 mixed solution Substances 0.000 claims description 11
230000003100 immobilizing effect Effects 0.000 claims description 9
229920002401 polyacrylamide Polymers 0.000 claims 1
HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 abstract description 17
239000000203 mixture Substances 0.000 abstract description 12
239000003431 cross linking reagent Substances 0.000 abstract description 10
239000000463 material Substances 0.000 abstract description 9
229920000642 polymer Polymers 0.000 abstract description 7
239000000126 substance Substances 0.000 abstract description 7
UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical compound C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 abstract description 6
YXFVVABEGXRONW-UHFFFAOYSA-N Toluene Chemical compound CC1=CC=CC=C1 YXFVVABEGXRONW-UHFFFAOYSA-N 0.000 abstract description 6
ROOXNKNUYICQNP-UHFFFAOYSA-N ammonium persulfate Chemical compound [NH4+].[NH4+].[O-]S(=O)(=O)OOS([O-])(=O)=O ROOXNKNUYICQNP-UHFFFAOYSA-N 0.000 abstract description 6
238000006116 polymerization reaction Methods 0.000 abstract description 6
239000003505 polymerization initiator Substances 0.000 abstract description 5
KWYHDKDOAIKMQN-UHFFFAOYSA-N N,N,N',N'-tetramethylethylenediamine Chemical compound CN(C)CCN(C)C KWYHDKDOAIKMQN-UHFFFAOYSA-N 0.000 abstract description 3
229910001870 ammonium persulfate Inorganic materials 0.000 abstract description 3
ZIUHHBKFKCYYJD-UHFFFAOYSA-N n,n'-methylenebisacrylamide Chemical compound C=CC(=O)NCNC(=O)C=C ZIUHHBKFKCYYJD-UHFFFAOYSA-N 0.000 abstract description 3
230000000379 polymerizing effect Effects 0.000 abstract description 3
235000012424 soybean oil Nutrition 0.000 abstract description 3
239000003549 soybean oil Substances 0.000 abstract description 3
108010093096 Immobilized Enzymes Proteins 0.000 abstract description 2
240000008415 Lactuca sativa Species 0.000 abstract description 2
239000004809 Teflon Substances 0.000 abstract description 2
229920006362 Teflon® Polymers 0.000 abstract description 2
BZHJMEDXRYGGRV-UHFFFAOYSA-N Vinyl chloride Chemical compound ClC=C BZHJMEDXRYGGRV-UHFFFAOYSA-N 0.000 abstract description 2
150000002148 esters Chemical class 0.000 abstract description 2
230000002209 hydrophobic effect Effects 0.000 abstract description 2
UBTYFVJZTZYJHZ-UHFFFAOYSA-N n-[2-(prop-2-enoylamino)propyl]prop-2-enamide Chemical compound C=CC(=O)NC(C)CNC(=O)C=C UBTYFVJZTZYJHZ-UHFFFAOYSA-N 0.000 abstract description 2
239000003921 oil Substances 0.000 abstract description 2
235000019198 oils Nutrition 0.000 abstract description 2
229920001296 polysiloxane Polymers 0.000 abstract description 2
USHAGKDGDHPEEY-UHFFFAOYSA-L potassium persulfate Chemical compound [K+].[K+].[O-]S(=O)(=O)OOS([O-])(=O)=O USHAGKDGDHPEEY-UHFFFAOYSA-L 0.000 abstract description 2
235000012045 salad Nutrition 0.000 abstract description 2
239000010802 sludge Substances 0.000 abstract description 2
RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 abstract 2
239000007788 liquid Substances 0.000 description 19
238000000926 separation method Methods 0.000 description 8
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 7
239000000654 additive Substances 0.000 description 5
230000000996 additive effect Effects 0.000 description 5
239000007864 aqueous solution Substances 0.000 description 5
238000006243 chemical reaction Methods 0.000 description 5
235000010443 alginic acid Nutrition 0.000 description 4
229920000615 alginic acid Polymers 0.000 description 4
239000000783 alginic acid Substances 0.000 description 3
229960001126 alginic acid Drugs 0.000 description 3
150000004781 alginic acids Chemical class 0.000 description 3
XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 description 3
239000000969 carrier Substances 0.000 description 2
239000003795 chemical substances by application Substances 0.000 description 2
238000000354 decomposition reaction Methods 0.000 description 2
238000010586 diagram Methods 0.000 description 2
230000000694 effects Effects 0.000 description 2
239000012530 fluid Substances 0.000 description 2
229910052751 metal Inorganic materials 0.000 description 2
239000002184 metal Substances 0.000 description 2
244000005700 microbiome Species 0.000 description 2
229920005615 natural polymer Polymers 0.000 description 2
229910052757 nitrogen Inorganic materials 0.000 description 2
239000007787 solid Substances 0.000 description 2
239000002904 solvent Substances 0.000 description 2
238000003756 stirring Methods 0.000 description 2
229920001059 synthetic polymer Polymers 0.000 description 2
238000004065 wastewater treatment Methods 0.000 description 2
FHVDTGUDJYJELY-UHFFFAOYSA-N 6-{[2-carboxy-4,5-dihydroxy-6-(phosphanyloxy)oxan-3-yl]oxy}-4,5-dihydroxy-3-phosphanyloxane-2-carboxylic acid Chemical compound O1C(C(O)=O)C(P)C(O)C(O)C1OC1C(C(O)=O)OC(OP)C(O)C1O FHVDTGUDJYJELY-UHFFFAOYSA-N 0.000 description 1
229920001817 Agar Polymers 0.000 description 1
239000004215 Carbon black (E152) Substances 0.000 description 1
JOYRKODLDBILNP-UHFFFAOYSA-N Ethyl urethane Chemical compound CCOC(N)=O JOYRKODLDBILNP-UHFFFAOYSA-N 0.000 description 1
241000605121 Nitrosomonas europaea Species 0.000 description 1
239000004372 Polyvinyl alcohol Substances 0.000 description 1
238000005273 aeration Methods 0.000 description 1
239000008272 agar Substances 0.000 description 1
229940072056 alginate Drugs 0.000 description 1
239000000679 carrageenan Substances 0.000 description 1
235000010418 carrageenan Nutrition 0.000 description 1
229920001525 carrageenan Polymers 0.000 description 1
229940113118 carrageenan Drugs 0.000 description 1
HGAZMNJKRQFZKS-UHFFFAOYSA-N chloroethene;ethenyl acetate Chemical compound ClC=C.CC(=O)OC=C HGAZMNJKRQFZKS-UHFFFAOYSA-N 0.000 description 1
238000004132 cross linking Methods 0.000 description 1
150000002170 ethers Chemical class 0.000 description 1
235000013305 food Nutrition 0.000 description 1
239000012634 fragment Substances 0.000 description 1
239000011521 glass Substances 0.000 description 1
229930195733 hydrocarbon Natural products 0.000 description 1
150000002430 hydrocarbons Chemical class 0.000 description 1
239000000178 monomer Substances 0.000 description 1
238000000465 moulding Methods 0.000 description 1
230000001546 nitrifying effect Effects 0.000 description 1
229920002451 polyvinyl alcohol Polymers 0.000 description 1
FVSKHRXBFJPNKK-UHFFFAOYSA-N propionitrile Chemical compound CCC#N FVSKHRXBFJPNKK-UHFFFAOYSA-N 0.000 description 1
230000035484 reaction time Effects 0.000 description 1
238000011084 recovery Methods 0.000 description 1
239000012266 salt solution Substances 0.000 description 1
150000003839 salts Chemical class 0.000 description 1
238000005406 washing Methods 0.000 description 1
239000002351 wastewater Substances 0.000 description 1
UHVMMEOXYDMDKI-JKYCWFKZSA-L zinc;1-(5-cyanopyridin-2-yl)-3-[(1s,2s)-2-(6-fluoro-2-hydroxy-3-propanoylphenyl)cyclopropyl]urea;diacetate Chemical compound [Zn+2].CC([O-])=O.CC([O-])=O.CCC(=O)C1=CC=C(F)C([C@H]2[C@H](C2)NC(=O)NC=2N=CC(=CC=2)C#N)=C1O UHVMMEOXYDMDKI-JKYCWFKZSA-L 0.000 description 1

Classifications

- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/10—Biological treatment of water, waste water, or sewage

Landscapes

Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Biological Treatment Of Waste Water (AREA)

JP61170247A 1986-07-18 1986-07-18 菌体または酵素の固定化方法 Granted JPS6324886A (ja)

Priority Applications (1)

Application Number	Priority Date	Filing Date	Title
JP61170247A JPS6324886A (ja)	1986-07-18	1986-07-18	菌体または酵素の固定化方法

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
JP61170247A JPS6324886A (ja)	1986-07-18	1986-07-18	菌体または酵素の固定化方法

Publications (2)

Publication Number	Publication Date
JPS6324886A true JPS6324886A (ja)	1988-02-02
JPH0560355B2 JPH0560355B2 (2)	1993-09-02

Family

ID=15901399

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
JP61170247A Granted JPS6324886A (ja)	1986-07-18	1986-07-18	菌体または酵素の固定化方法

Country Status (1)

Country	Link
JP (1)	JPS6324886A (2)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
JP2018504137A (ja) *	2015-02-03	2018-02-15	上▲海▼▲凱▼▲賽▼生物技▲術▼研▲發▼中心有限公司	固定化細胞及びその製造方法

1986
- 1986-07-18 JP JP61170247A patent/JPS6324886A/ja active Granted

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
JP2018504137A (ja) *	2015-02-03	2018-02-15	上▲海▼▲凱▼▲賽▼生物技▲術▼研▲發▼中心有限公司	固定化細胞及びその製造方法
US10619148B2 (en)	2015-02-03	2020-04-14	Cathay Biotech Inc.	Immobilized cell and preparation method thereof

Also Published As

Publication number	Publication date
JPH0560355B2 (2)	1993-09-02

Publication	Publication Date	Title
US4110529A (en)	1978-08-29	Method of manufacturing spherical polymer particles from polymer solutions
Kim et al.	2000	Removal of ammonium-N from a recirculation aquacultural system using an immobilized nitrifier
JP3839136B2 (ja)	2006-11-01	微生物固定化磁性担体、その製造方法及び廃水処理方法
CN103642787B (zh)	2017-03-15	光合细菌微胶囊的制备方法
DE3718934A1 (de)	1987-12-17	Verfahren und vorrichtung zur herstellung von polymer-perlen
JPS59154999A (ja)	1984-09-04	生物化学反応方法および生物化学反応装置
JPH10136980A (ja)	1998-05-26	バイオリアクタ−用担体及び製造方法
JPS6329997B2 (2)	1988-06-16
JPS6324886A (ja)	1988-02-02	菌体または酵素の固定化方法
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JPH0210716B2 (2)	1990-03-09
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JPH0618582Y2 (ja)	1994-05-18	凝固装置