JPS6345540A - Constant temperature holding device for chemical analysis - Google Patents
Constant temperature holding device for chemical analysisInfo
- Publication number
- JPS6345540A JPS6345540A JP16551387A JP16551387A JPS6345540A JP S6345540 A JPS6345540 A JP S6345540A JP 16551387 A JP16551387 A JP 16551387A JP 16551387 A JP16551387 A JP 16551387A JP S6345540 A JPS6345540 A JP S6345540A
- Authority
- JP
- Japan
- Prior art keywords
- chemical analysis
- constant temperature
- slide
- sealing
- hermetic sealing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000000126 substance Substances 0.000 title claims abstract description 98
- 238000004458 analytical method Methods 0.000 title claims abstract description 92
- 238000007789 sealing Methods 0.000 claims abstract description 46
- 239000000463 material Substances 0.000 claims abstract description 30
- 230000007246 mechanism Effects 0.000 claims abstract description 23
- 230000007723 transport mechanism Effects 0.000 claims description 16
- 239000007788 liquid Substances 0.000 claims description 14
- 238000012423 maintenance Methods 0.000 claims description 7
- 230000032258 transport Effects 0.000 claims 1
- 238000010438 heat treatment Methods 0.000 abstract description 11
- 238000001816 cooling Methods 0.000 abstract description 3
- 229910052751 metal Inorganic materials 0.000 abstract description 2
- 239000002184 metal Substances 0.000 abstract description 2
- 239000004033 plastic Substances 0.000 abstract description 2
- 239000000523 sample Substances 0.000 description 11
- 229920001343 polytetrafluoroethylene Polymers 0.000 description 7
- 239000004810 polytetrafluoroethylene Substances 0.000 description 7
- 239000003153 chemical reaction reagent Substances 0.000 description 6
- 230000000694 effects Effects 0.000 description 6
- 238000012360 testing method Methods 0.000 description 5
- 238000011161 development Methods 0.000 description 4
- 238000001704 evaporation Methods 0.000 description 4
- 238000003780 insertion Methods 0.000 description 4
- 230000037431 insertion Effects 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 230000003287 optical effect Effects 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 3
- 229910052802 copper Inorganic materials 0.000 description 3
- 239000010949 copper Substances 0.000 description 3
- 230000035484 reaction time Effects 0.000 description 3
- 239000012491 analyte Substances 0.000 description 2
- 239000011248 coating agent Substances 0.000 description 2
- 238000000576 coating method Methods 0.000 description 2
- 229920001971 elastomer Polymers 0.000 description 2
- 230000008020 evaporation Effects 0.000 description 2
- 239000012466 permeate Substances 0.000 description 2
- -1 polytetrafluoroethylene Polymers 0.000 description 2
- 238000003892 spreading Methods 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 238000012351 Integrated analysis Methods 0.000 description 1
- 230000005679 Peltier effect Effects 0.000 description 1
- PNNCWTXUWKENPE-UHFFFAOYSA-N [N].NC(N)=O Chemical compound [N].NC(N)=O PNNCWTXUWKENPE-UHFFFAOYSA-N 0.000 description 1
- 229910052782 aluminium Inorganic materials 0.000 description 1
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 238000004040 coloring Methods 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 239000003365 glass fiber Substances 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 230000008531 maintenance mechanism Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 238000005375 photometry Methods 0.000 description 1
- 239000012488 sample solution Substances 0.000 description 1
- 229920002379 silicone rubber Polymers 0.000 description 1
- 239000004945 silicone rubber Substances 0.000 description 1
- 238000004381 surface treatment Methods 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
- 239000011800 void material Substances 0.000 description 1
Landscapes
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
Abstract
Description
【発明の詳細な説明】
（産業上の利用分野）
本発明は、化学分析用恒温保持装置に関し、詳細には多
層化学分析スライド、特に多層化学分析スライドの試薬
層中の呈色試薬に被測定物質を作用させ、その呈色度合
を光学的手段等により高精度の定量又は判定を行う為、
上記化学分析スライドを密閉して能率：く所定時間恒温
保持することのできる化学分析用恒温保持装置に関する
ものである。DETAILED DESCRIPTION OF THE INVENTION (Field of Industrial Application) The present invention relates to a constant temperature holding device for chemical analysis. In order to quantify or judge the degree of coloration with high precision by applying a substance and using optical means, etc.
The present invention relates to a constant temperature holding device for chemical analysis, which can efficiently hold the chemical analysis slide at a constant temperature for a predetermined time while hermetically sealing it.
（従来の技術）
従来より、例えば血液中に含まれるグルコースｅ度、尿
素窒素濃度などの成分濃度を判定する試験においては、
最も簡便な分析手段として呈色試験紙を用い、その呈色
試験色の呈色度合を被測定物質の標準濃度における呈色
色見本と視覚的に対比を行い判定するものがあった。し
かし、上記の分析手段においては、呈色試験紙の呈色度
合の判定を人間の視覚的判断に依存するものであった為
、その精度において満足のゆくものではなかった。(Prior Art) Conventionally, in tests to determine the concentration of components contained in blood, such as glucose concentration and urea nitrogen concentration,
The simplest analytical means used was to use a color test paper and visually compare the degree of color development of the test color with a color sample at a standard concentration of the substance to be measured. However, in the above-mentioned analysis means, since the determination of the degree of coloration of the coloration test paper was dependent on human visual judgment, the accuracy thereof was not satisfactory.
近代医学の発展とともに、より精度の高い分析判定が要
求されるに至り、少なくとも展開層及び試薬層の二層を
有する多層化学分析材料（フィルム）をスライド枠に収
め念多層化学分析スライドを用い、光学的手段により自
動的に分析する方法及び装置が種々提案されている。こ
の分析方法は、被測定物質を含む水性液体試料を所定量
、上記多１１化学分析スライドに計量付着させ、所定時
間恒温保持した後に、被測定物質と上記試薬層て含まれ
る試薬との組み合せ１（より予め選定された波長を含む
測定用の照射光を該多層化学分析スライドに対し照射し
、その反射光学濃度又は透過光学濃度を測定するととく
より、被測定物質の濃度を測定しようとするものである
。With the development of modern medicine, more accurate analytical judgments are required, and a multilayer chemical analysis slide is used to house a multilayer chemical analysis material (film) that has at least two layers, a development layer and a reagent layer, in a slide frame. Various methods and devices for automatic analysis using optical means have been proposed. In this analysis method, a predetermined amount of an aqueous liquid sample containing a substance to be measured is weighed and deposited on the chemical analysis slide described above, and after being kept at a constant temperature for a predetermined period of time, a combination of the substance to be measured and a reagent contained in the reagent layer 1 is prepared. (Measurement irradiation light containing a wavelength selected in advance is irradiated onto the multilayer chemical analysis slide, and its reflected optical density or transmitted optical density is measured, and in particular, the concentration of the substance to be measured is to be measured. It is.
尚、多層分析材料の具体例としては、たとえば特開昭４
９−５３８８８号、特開昭５０−１３７１９２号、特開
昭５１−４０１９１号、ｆＦ開昭５２−３４８８号、特
開昭５２−１３１７８６号、特開昭５２−１４２５８４
号、ｆＦ間昭５５−３３６５１号、特開昭５５−１６４
３５６号、・￥ｆ開昭５６−８５４９号、特開昭５６−
９６２４５号、特開昭５６−９７８７２号などの各明細
書に記載されている多層一体型分析材料がある。これら
の多層分析材料は水不透過性支持体上に１層または複数
層の試薬層と多孔性展開層とを積層し一体化したフィル
ム状の構造のものである。このようなフィルム状分析材
料の最外層（多孔性展開層）に一定容量の液体試料が滴
下されると、溶液はほぼ一定面積に展開しながら、下層
へ浸透していき、そこで、被検体量に比例するように設
定された反応、例えば発色反応が進行する。一定時間後
の発色濃度の測光により液体試料中の被検物質の量を検
出、定量する。このようなフィルム状分析材料の特徴は
、その最外層が試料溶液をほぼ均一に展開する特性を有
する多孔性膜でおおわれていることであって、その特性
及び材料に関しては特開昭４９−５３８８８号あるいは
特開昭５５−１６４３５６号などに詳述されている通り
である。In addition, as a specific example of a multilayer analysis material, for example,
9-53888, JP 50-137192, JP 51-40191, fF JP 52-3488, JP 52-131786, JP 52-142584
No., fFma No. 55-33651, Japanese Patent Publication No. 55-164
No. 356, ¥f 8549/1983, JP-A-56-
There are multilayer integrated analysis materials described in specifications such as No. 96245 and Japanese Patent Application Laid-Open No. 56-97872. These multilayer analysis materials have a film-like structure in which one or more reagent layers and a porous development layer are laminated and integrated on a water-impermeable support. When a certain volume of liquid sample is dropped onto the outermost layer (porous spreading layer) of such a film-like analysis material, the solution spreads over a nearly constant area and permeates into the lower layer, where the amount of analyte increases. A reaction, such as a coloring reaction, proceeds in proportion to . The amount of the test substance in the liquid sample is detected and quantified by photometry of the color density after a certain period of time. A feature of such a film-like analytical material is that its outermost layer is covered with a porous membrane that has the property of spreading the sample solution almost uniformly. No. 55-164356 or the like.
（発明が解決しようとする問題点）
上述の多層化学分析スライドの恒温保持に際しては、従
来より恒温器等の手段が用いられてきたが、この場合、
恒温保持中に該多層化学分析スライドから水分が蒸発し
、被測定物質と試薬との反応時間中水分の量を一定に保
つことができなかった。その結果、判定誤差を招き、精
度の高い分析判定結果を得ることができないものであっ
た。そこで、上記従来袋＃における欠点を鑑みて、該多
層化学分析スライドの多層化学分析材料（フィルム）を
恒温保持処理を行う間外界と遮断する目的で、該多層分
析スライドに密閉用のカバーを設ける使用方法（特開昭
５６−１０１５３７号発明）も考えられ念が、使い捨て
である該多層化学分析スライドの構造が複雑となり、生
産コストの上昇を招くとともに１恒温保持処理における
手数が増え、分析判定における自動化の防げとなるもの
であり、更に１被測定物質が汚染されやすいう欠点もあ
った。(Problems to be Solved by the Invention) Conventionally, means such as a thermostat have been used to maintain the above-mentioned multilayer chemical analysis slide at a constant temperature.
Water evaporated from the multilayer chemical analysis slide during constant temperature maintenance, and the amount of water could not be kept constant during the reaction period between the analyte and the reagent. As a result, judgment errors occur, making it impossible to obtain highly accurate analysis and judgment results. Therefore, in view of the drawbacks of the conventional bag # mentioned above, an airtight cover is provided on the multilayer chemical analysis slide in order to isolate the multilayer chemical analysis material (film) of the multilayer chemical analysis slide from the outside world during constant temperature holding treatment. However, the structure of the disposable multilayer chemical analysis slide becomes complicated, which increases the production cost and increases the number of steps required for constant temperature maintenance, making it difficult to make analytical decisions. This prevents automation in the process, and also has the disadvantage that one of the substances to be measured is likely to be contaminated.
（問題点を解決するための手段）
本発明は、前述の化学分析における恒温保持方法及び装
置の欠点を鑑みて成されたもので、その目的は簡単な構
造を有し、恒温保持処理における、多量化学分析スライ
ドの被測定物質を含む水性液体試料が計量付着された多
層化学分析材料からの水分の蒸発を積極的に防止し、反
応時間における水分の量を一定に保つことができ、更；
て恒温保持時間も任意に設定することのできる化学分析
用恒温保持装置を提供することにある。(Means for Solving the Problems) The present invention was made in view of the drawbacks of the above-mentioned constant temperature maintenance method and apparatus for chemical analysis, and its purpose is to have a simple structure and to It is possible to actively prevent the evaporation of moisture from the multilayer chemical analysis material to which the aqueous liquid sample containing the substance to be measured of the multilayer chemical analysis slide is weighed and adhered, and to keep the amount of moisture constant during the reaction time, and further;
An object of the present invention is to provide a constant temperature holding device for chemical analysis in which the constant temperature holding time can also be arbitrarily set.
本発明は、■一対の搬送ローラ、及びその周囲に巻掛さ
れた搬送ベルトからなり、搬送ベルトが水性液体試料が
付着されて被測定物質が浸透した層を少なくとも一層含
む多層化学分析材料を収めた枠からなる化学分析スライ
ドを搬送する搬送機構、
■一対の密閉ガイドベルトローラー、及びその周囲に巻
掛された密閉ガイドベルトからなり、密閉ガイドベルト
が化学分析スライドの枠の表面に密着又は接触して多層
化学分析材料の水性液体試料が付着された側（密閉空間
を形成する密閉機構、■前記搬送機構を駆動する駆動機
構、及び■前記化学分析スライドを恒温保持する恒温保
持機構、
を有することを特徴とする化学分析用恒温保持装置であ
る。The present invention consists of: (1) a pair of conveyor rollers and a conveyor belt wound around them; A transport mechanism for transporting a chemical analysis slide consisting of a frame, ■ A pair of sealed guide belt rollers and a sealed guide belt wrapped around them, the sealed guide belt being in close contact with or in close contact with the surface of the frame of the chemical analysis slide. and a side of the multilayer chemical analysis material to which the aqueous liquid sample is attached (a sealing mechanism that forms a sealed space, a drive mechanism that drives the transport mechanism, and a constant temperature maintenance mechanism that maintains the chemical analysis slide at a constant temperature. This is a constant temperature holding device for chemical analysis.
また、本発明は、■円板状の搬送部材からなり、搬送部
材が水性液体試料が付着されて被測定物質が浸透した層
を少なくとも一層含む多層化学分析材料を収めた枠から
なる化学分析スライドを搬送する搬送機構、
■切りかき部を有する円板状密閉部材からなり、密閉部
材が化学分析スライドの枠の表面に密着又は接触して多
層化学分析材料の水性液体試料が付着された側に密閉空
間を形成し、前記切りかき部が化学分析スライドを出し
入れするための空所を形成する密閉機構、
■前記搬送機構を駆動する駆動機構、及び■前記化学分
析スライドを恒温保持する恒温保持機構、
を有することを特徴とする化学分析用恒温保持装置であ
る。In addition, the present invention provides: (1) A chemical analysis slide consisting of a disc-shaped transport member, the transport member comprising a frame containing a multilayer chemical analysis material including at least one layer to which an aqueous liquid sample is attached and a substance to be measured permeated; A transport mechanism for transporting the material, ■ a disc-shaped sealing member having a notch, the sealing member being in close contact with or in close contact with the surface of the frame of the chemical analysis slide, on the side of the multilayer chemical analysis material to which the aqueous liquid sample has been attached. a sealing mechanism that forms a sealed space, and the cut portion forms a void for loading and unloading the chemical analysis slide; (1) a drive mechanism that drives the transport mechanism; and (2) a constant temperature holding mechanism that maintains the chemical analysis slide at a constant temperature. A constant temperature holding device for chemical analysis is characterized by having the following.
（実施例） 以下本発明の実施例を図面に基づいて詳細に説明する。(Example) Embodiments of the present invention will be described in detail below based on the drawings.
第１図は、本発明の化学分析用恒温保持装置の一実施例
の主要部を示す櫃略斜視図であり、図中、１．１′は駆
動用電動機（図示せず）により駆動される一対の多層化
学分析用スライド搬送用の搬送ローラーであシ、その周
囲に巻掛された熱伝導率が高く、塑性変形しにくい耐久
性に富む材質から成る搬送ベルト３と共に多層化学分析
スライド搬送機構（以下、搬送機構とする。）を構成す
るものである。２．２′は、一対の密閉ガイドベルトロ
ーラーであり、その周囲にけ被測定物質が浸透した層を
密閉する密閉機構をなし、同時に多層化学分析スライド
のガイドをなす密閉用ガイドベルト４が巻掛されており
、多層化学分析スライド密閉機構（以下、密閉機構とす
る。）を構成している。FIG. 1 is a schematic perspective view showing the main parts of an embodiment of the constant temperature holding device for chemical analysis of the present invention. In the figure, 1.1' is driven by a driving electric motor (not shown). A pair of transport rollers for transporting multilayer chemical analysis slides, a transport belt 3 made of a highly durable material with high thermal conductivity and resistant to plastic deformation wrapped around the rollers, and a multilayer chemical analysis slide transport mechanism. (hereinafter referred to as the transport mechanism). 2.2' is a pair of sealing guide belt rollers, around which a sealing guide belt 4 is wound which forms a sealing mechanism for sealing the layer into which the substance to be measured has permeated, and at the same time serves as a guide for the multilayer chemical analysis slide. It forms a multilayer chemical analysis slide sealing mechanism (hereinafter referred to as the sealing mechanism).
５は、多層化学分析スライド８を所定時間恒温保持する
為建設けられた加熱用もしくは加熱・冷却用の電気素子
を内部に埋設したアルミニウム、銅等の熱伝導率のよい
金属からなる恒温保持手段である。この電気素子として
は、例えば、加熱用ヒーター、ペルチェ効果を利用した
冷却用素子等がある。そして、７は上記各部材を収納保
護する為のケースである。以下、上記電気素子の内、加
熱用ヒーターを多層化学分析スライド８の恒温保持手段
として用いた実施例について詳述する。5 is a constant temperature holding means made of a metal with good thermal conductivity such as aluminum or copper, which is constructed to hold the multilayer chemical analysis slide 8 at a constant temperature for a predetermined period of time and has an electric element for heating or heating/cooling embedded therein. It is. Examples of this electric element include a heating heater and a cooling element that utilizes the Peltier effect. And 7 is a case for storing and protecting each of the above-mentioned members. Hereinafter, an example in which a heating heater among the above-mentioned electric elements is used as a constant temperature maintenance means for the multilayer chemical analysis slide 8 will be described in detail.
第２図は本発明の搬送及び密閉機構の第１の実施例を示
す側面図である。第６図は第２図の要部拡大図であって
化学分析スライドの被測定物質が浸透した層が密閉され
つつ恒温保持され搬送される概念図である。８１はフィ
ルム状多層化学分析材料、８２はスライド枠、８４は空
間、８５は水性液体試料を示す。また、第３図及び第４
図は、それぞれ本発明の搬送機構の第２、第３の実施例
を示す側面図である。図中、第１図と同一部材を示すも
のは同一の参照番号で示されている。FIG. 2 is a side view showing a first embodiment of the conveyance and sealing mechanism of the present invention. FIG. 6 is an enlarged view of the main part of FIG. 2, and is a conceptual diagram in which the layer of the chemical analysis slide in which the substance to be measured permeates is sealed, maintained at a constant temperature, and transported. 81 is a film-like multilayer chemical analysis material, 82 is a slide frame, 84 is a space, and 85 is an aqueous liquid sample. Also, Figures 3 and 4
The figures are side views showing second and third embodiments of the transport mechanism of the present invention, respectively. In the drawings, the same members as in FIG. 1 are designated by the same reference numerals.
第２図からも明らかなように１加熱用ヒーター５は搬送
用ベルト３の近傍に設けられており、多層化学分析スラ
イド８は該＾送ベルト３を介し加熱される。次に、密閉
用ガイドベルトロー２２゜２′は、その周囲に巻掛され
た密閉用ガイドベルト４が、多層化学分析スライド搬送
時に常に多層化学分析スライド８のスライド枠の表面と
図示の如く密着して密閉するようにケース７に回転自在
に軸着されている。密閉用ガイドベルトの素材としては
、ポリテトラフルオロエチレン（ＰＴＦＥ）シート、ガ
ラス繊維等のシートにＰＴＦＥを含浸または積層したシ
ート、シリコーンゴムシート等を用いることができる。As is clear from FIG. 2, the heating heater 5 is provided near the conveyor belt 3, and the multilayer chemical analysis slide 8 is heated via the conveyor belt 3. Next, the sealing guide belt row 22゜2' is such that the sealing guide belt 4 wound around it is always in close contact with the surface of the slide frame of the multilayer chemical analysis slide 8 as shown in the figure when the multilayer chemical analysis slide is transported. It is rotatably attached to the case 7 so as to be hermetically sealed. As a material for the sealing guide belt, a polytetrafluoroethylene (PTFE) sheet, a sheet of glass fiber impregnated with or laminated with PTFE, a silicone rubber sheet, etc. can be used.
第６図（第２図の要部拡大図）から明らかなように、多
層化学分析材料（フィルム）は密閉用ガイドベルトによ
って密閉され、スライド枠と密閉用ガイドベルトによっ
て多層化学分析材料の水性液体試料が付着された側に密
閉された空間８４が形成されている。密閉された空間の
作用効果は特開昭５ｆｉ−１０１５３７号明細書に詳細
に説明されている。図中、矢印は各ローラー及びベルト
の回転方向及び多層化学分析スライド８の搬送方向を示
すものである。第２図で示す第１の実施例では、上述の
密閉用ガイドベルト４と多層化学分析スライド８のスラ
イド枠の表面との密閉効果をよシ一層高める為にガイド
部材６が設けられている。As is clear from Figure 6 (an enlarged view of the main part of Figure 2), the multilayer chemical analysis material (film) is sealed by the sealing guide belt, and the aqueous liquid of the multilayer chemical analysis material is sealed by the slide frame and the sealing guide belt. A sealed space 84 is formed on the side to which the sample is attached. The effects of a closed space are explained in detail in Japanese Patent Application Laid-open No. 5FI-101537. In the figure, arrows indicate the direction of rotation of each roller and belt and the direction of conveyance of the multilayer chemical analysis slide 8. In the first embodiment shown in FIG. 2, a guide member 6 is provided in order to further enhance the sealing effect between the above-mentioned sealing guide belt 4 and the surface of the slide frame of the multilayer chemical analysis slide 8.
このガイド部材６及び前記加熱用ヒーター５は相方とも
に密閉用ガイドベルト４及び搬送ベルト３と面接触する
為、その接触摩擦抵抗を小さくするようにＰＴＦＥコー
ティング加工等の表面処理を施したものを用いることが
できる。尚、該ガイド部材６に、加熱効果を高める為、
内部に加熱用ヒーターが埋設されたものを使用し上下両
方から加熱し、きめ細かな温度制御を行ってもよいこと
は言うまでもない。Since both the guide member 6 and the heating heater 5 make surface contact with the sealing guide belt 4 and the conveyor belt 3, they should be surface-treated with PTFE coating to reduce the frictional resistance of the contact. be able to. In addition, in order to enhance the heating effect on the guide member 6,
Needless to say, a device with a heating heater embedded inside may be used to heat from both the top and bottom and perform fine temperature control.
上記第１の実施例は、密閉用ガイドベルト及びガイド部
材６の両方を具備しているが、恒温搬送時に、多層化学
分析材料からの水分の蒸発を防止するように多層化学分
析スライド８のスライド枠の表面を密閉できるように構
成されるならば、必ずしも密閉用ガイドベルト及びガイ
ド部材６の両方を必要とするものではない。また、必要
に応じて、該ガイド部材６を多層化学分析スライド８の
方向に、密閉効果を高める為適当な付勢手段により付勢
してもよい。The first embodiment is equipped with both the sealing guide belt and the guide member 6, but the slide of the multilayer chemical analysis slide 8 is designed to prevent moisture from evaporating from the multilayer chemical analysis material during constant temperature transport. As long as the surface of the frame is configured to be sealed, both the sealing guide belt and the guide member 6 are not necessarily required. Further, if necessary, the guide member 6 may be biased toward the multilayer chemical analysis slide 8 by suitable biasing means to enhance the sealing effect.
次に、第３図、第４図に従って、本発明の搬送機構の第
２、第３の実施例、特に搬送ベルト３の構造について説
明する。Next, second and third embodiments of the conveying mechanism of the present invention, particularly the structure of the conveying belt 3, will be described with reference to FIGS. 3 and 4.
第２図で示された第１の実施例では、搬送ベルト３は、
通常用いられる平型の搬送ベルトであったが、第３図で
示す第２の実施例においては、図示の如く、多層化学分
析スライド８を確実に搬送するようにベルト表面に複数
の多層化学分析スライド挿入凹部９を設けた熱伝導性の
高いゴム、ＰＴＦＥ、銅などの金銅にＰＴＦＥやゴムな
どを積層したシート等のベルトが搬送ベルト３として用
いられている。この場合、搬送が確実になる。In the first embodiment shown in FIG. 2, the conveyor belt 3 is
Although this was a commonly used flat conveyor belt, in the second embodiment shown in FIG. The conveyor belt 3 is made of highly thermally conductive rubber, PTFE, or a sheet made of gilt copper such as copper and laminated with PTFE, rubber, or the like, provided with a slide insertion recess 9 . In this case, transportation becomes reliable.
第４図に示す第３の実施例は、第２の実施例の凹部９の
かわシに、多層化学分析スライド８を搬送方向へ押す島
状もしくは畝状の凸部１０を有する搬送ベルト３を用い
るものである。この効果としては、四角形の外形を有す
る多層化学分析スライド搬送時には、はぼ第２の実施例
と同様のものである。A third embodiment shown in FIG. 4 includes a conveyor belt 3 having an island-like or ridge-like convex portion 10 for pushing the multilayer chemical analysis slide 8 in the conveyance direction in place of the concave portion 9 of the second embodiment. It is used. This effect is similar to that of the second embodiment when transporting a multilayer chemical analysis slide having a rectangular outer shape.
以上説明してきた第１〜第３の実施例において、多層化
学分析材料８の恒温保持時間については、搬送機構駆動
用の電動機の回転速度を制御することによシ、被測定物
質と試薬との必要反応時間に従って容易に決定できるも
のであり、また、その恒温保持時間は常に一定のものが
得られる。In the first to third embodiments described above, the constant temperature holding time of the multilayer chemical analysis material 8 can be controlled by controlling the rotational speed of the motor for driving the transport mechanism. It can be easily determined according to the required reaction time, and the constant temperature holding time can always be obtained.
更に他の搬送機構の実施例としては、第５図に示すよう
な複数の多層化学分析スライド挿入凹部を有する円板状
の搬送部材２０がある。この搬送部材２０は円板状の加
熱用ヒーター３０の上に回転自在に軸着され、上述の実
施例同様図示しない電動機によシ駆動されるものであり
、恒温保持時間は、この電動機の速度制御を行うことで
、任意に、一定時間に設定することができる。該円板状
の搬送部材２０の上には、搬送される多層化学分析スラ
イド８の上部と接触し、多層化学分析材料からの水分の
蒸発を防止する目的で、密閉部材４０が設けられている
。該密閉１部材４０には、多層化学分析スライド８を出
し入れする為の切りかき部が設けられている。この実施
例においても、多層化学分析スライドのスライド枠の表
面と接触する密閉部材４０の表面は、接触摩擦抵抗の減
小のために、表面処理（例、ポリテトラフルオロエチレ
ンのコーティング）を施した方が好ましい。また、密閉
部材４０にも加熱用ヒーターを設は両面よシ多層化学分
析スライドを加熱してもよいものである。Still another example of the transport mechanism is a disc-shaped transport member 20 having a plurality of multilayer chemical analysis slide insertion recesses as shown in FIG. This conveying member 20 is rotatably mounted on a disk-shaped heater 30, and is driven by an electric motor (not shown) as in the above-mentioned embodiment, and the constant temperature maintenance time is determined by the speed of this electric motor. By performing control, it is possible to arbitrarily set a certain period of time. A sealing member 40 is provided on the disc-shaped transport member 20 for the purpose of contacting the upper part of the multilayer chemical analysis slide 8 being transported and preventing moisture from evaporating from the multilayer chemical analysis material. . The sealing member 40 is provided with a cutout for inserting and removing the multilayer chemical analysis slide 8. In this embodiment as well, the surface of the sealing member 40 that comes into contact with the surface of the slide frame of the multilayer chemical analysis slide is subjected to surface treatment (e.g., polytetrafluoroethylene coating) to reduce contact friction resistance. is preferable. Further, a heating heater may be provided in the sealing member 40 to heat the multilayer chemical analysis slide on both sides.
尚、前述の実施例における搬送機構のベルト幅、多層化
学分析スライド挿入凹凸部の個数及び形状、そして材質
等は実施例において開示したものに限られるものではな
く、用途に応じて変化することのできるものである。It should be noted that the belt width of the conveyance mechanism, the number and shape of the multilayer chemical analysis slide insertion irregularities, the material, etc. in the above-mentioned embodiments are not limited to those disclosed in the embodiments, and may vary depending on the application. It is possible.
（発明の効果）
以上の説明からも明らかなように、本発明の化学分析用
恒温保持装置においては、恒温保持処理中、多層化学分
析スライドの多層化学分析材料が、密閉用ガイドベルト
、密閉用ガイド部材又は密閉部材等により常に密閉され
ている為、被測定物質と試薬の反応時間における水分の
蒸発が積極的に防止され、その結果、水分の量を一定に
保つことができ、精度の高い分析・判定を行うことがで
きる。更Ｋ、恒温保持時間についても、搬送機構駆動用
の電動機の回転速度制御により、簡単かつ正確に設定す
ることができる。(Effects of the Invention) As is clear from the above explanation, in the constant temperature holding device for chemical analysis of the present invention, during the constant temperature holding process, the multilayer chemical analysis material of the multilayer chemical analysis slide is Since it is always sealed with a guide member or sealing member, evaporation of water during the reaction time of the substance to be measured and the reagent is actively prevented, and as a result, the amount of water can be kept constant, resulting in high accuracy. Able to analyze and judge. Additionally, the constant temperature holding time can be easily and accurately set by controlling the rotational speed of the electric motor for driving the transport mechanism.
第１図は、本発明の化学分析用恒温保持装置の一実施例
の主要部を示す概略斜視図、第２図は、本発明の搬送及
び密閉機構の第１の実施例を示す側面図、第３図は、本
発明の搬送機構の第２の実施例を示す側面図、第４図は
、本発明の搬送機構の第３の実施例を示す側面図、そし
て、第５図は、本発明の化学分析用恒温保持装置の他の
実施例の主要部を示す概略斜視図、第６図は第２図に示
す搬送及び密閉機構の要部拡大図。
１．１′・・・搬送ローラー、２．２′・・・密閉用ガ
イドベルトローラー、３・・−搬送ベルト、４・・・密
閉用ガイドベルト、５・・・恒温保持手段、６・・・ガ
イド部材、７・・・ケース、８・−多層化学分析スライ
ド、９・・・多層化学分析スライド挿入凹部、１０・−
多層化学分析スライド搬送用凸部、２０・・・円板状搬
送部材、３０・−円板状加熱用ヒーター、４０・・・密
閉部材、８］−フィルム状多層化学分析材料、８２・・
・スライド枠、８４・−・空間、８５・・・水性液体試
料。
第 ２［］
第 ４（２Ｉ
第５図FIG. 1 is a schematic perspective view showing the main parts of an embodiment of the constant temperature holding device for chemical analysis of the present invention, and FIG. 2 is a side view showing the first embodiment of the conveying and sealing mechanism of the present invention. FIG. 3 is a side view showing a second embodiment of the transport mechanism of the present invention, FIG. 4 is a side view showing a third embodiment of the transport mechanism of the invention, and FIG. FIG. 6 is a schematic perspective view showing the main parts of another embodiment of the constant temperature holding device for chemical analysis of the invention, and FIG. 6 is an enlarged view of the main parts of the conveyance and sealing mechanism shown in FIG. 2. 1.1'... Conveyance roller, 2.2'... Sealing guide belt roller, 3...-Transporting belt, 4... Sealing guide belt, 5... Constant temperature maintenance means, 6...・Guide member, 7... Case, 8... Multilayer chemical analysis slide, 9... Multilayer chemical analysis slide insertion recess, 10...
Convex portion for transporting a multilayer chemical analysis slide, 20... Disc-shaped transport member, 30... Disc-shaped heater, 40... Sealing member, 8] - Film-shaped multilayer chemical analysis material, 82...
- Slide frame, 84... Space, 85... Aqueous liquid sample. 2nd [] 4th (2I Fig. 5
Claims (1)
れた搬送ベルトからなり、搬送ベルトが水性液体試料が
付着されて被測定物質が浸透した層を少なくとも一層含
む多層化学分析材料を収めた枠からなる化学分析スライ
ドを搬送する搬送機構、（２）一対の密閉ガイドベルト
ローラー、及びその周囲に巻掛された密閉ガイドベルト
からなり、密閉ガイドベルトが化学分析スライドの枠の
表面に密着又は接触して多層化学分析材料の水性液体試
料が付着された側に密閉空間を形成する密閉機構、 （３）前記搬送機構を駆動する駆動機構、及び（４）前
記化学分析スライドを恒温保持する恒温保持機構、 を有することを特徴とする化学分析用恒温保持装置。 ２ （１）円板状の搬送部材からなり、搬送部材が水性
液体試料が付着されて被測定物質が浸透した層を少なく
とも一層含む多層化学分析材料を収めた枠からなる化学
分析スライドを搬送する搬送機構、 （２）切りかき部を有する円板状密閉部材からなり、密
閉部材が化学分析スライドの枠の表面に密着又は接触し
て多層化学分析材料の水性液体試料が付着された側に密
閉空間を形成し、前記切りかき部が化学分析スライドを
出し入れするための空所を形成する密閉機構、 （３）前記搬送機構を駆動する駆動機構、及び（４）前
記化学分析スライドを恒温保持する恒温保持機構、 を有することを特徴とする化学分析恒温保持装置。[Scope of Claims] 1 (1) A multilayer structure consisting of a pair of conveyance rollers and a conveyance belt wound around the conveyance belt, the conveyance belt including at least one layer to which an aqueous liquid sample is attached and a substance to be measured is permeated therein. A transport mechanism that transports a chemical analysis slide consisting of a frame containing chemical analysis materials; (2) a pair of sealed guide belt rollers; and a sealed guide belt wrapped around the rollers; a sealing mechanism that forms a sealed space on the side of the multilayer chemical analysis material to which the aqueous liquid sample is attached by closely or in contact with the surface of the frame; (3) a drive mechanism that drives the transport mechanism; and (4) a drive mechanism that drives the chemical analysis. A constant temperature holding device for chemical analysis, comprising: a constant temperature holding mechanism for keeping a slide at a constant temperature. 2 (1) A chemical analysis slide consisting of a disk-shaped conveying member, which is a frame containing a multilayer chemical analysis material including at least one layer to which an aqueous liquid sample is attached and in which the substance to be measured is permeated. A transport mechanism, (2) consisting of a disc-shaped sealing member having a notch, the sealing member being in close contact with or in contact with the surface of the frame of the chemical analysis slide, and sealing the side of the multilayer chemical analysis material to which the aqueous liquid sample is attached. a sealing mechanism that forms a space and the cut portion forms a cavity for loading and unloading the chemical analysis slide; (3) a drive mechanism that drives the transport mechanism; and (4) a constant temperature maintenance of the chemical analysis slide. A chemical analysis constant temperature holding device characterized by having a constant temperature holding mechanism.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP16551387A JPS6345540A (en) | 1987-07-03 | 1987-07-03 | Constant temperature holding device for chemical analysis |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP16551387A JPS6345540A (en) | 1987-07-03 | 1987-07-03 | Constant temperature holding device for chemical analysis |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS6345540A true JPS6345540A (en) | 1988-02-26 |
| JPS644143B2 JPS644143B2 (en) | 1989-01-24 |
Family
ID=15813824
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP16551387A Granted JPS6345540A (en) | 1987-07-03 | 1987-07-03 | Constant temperature holding device for chemical analysis |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS6345540A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0269761U (en) * | 1988-11-15 | 1990-05-28 | ||
| JPH02143166A (en) * | 1988-11-25 | 1990-06-01 | Olympus Optical Co Ltd | Automatic transporting device for biosample |
| US5122441A (en) * | 1990-10-29 | 1992-06-16 | E. I. Du Pont De Nemours And Company | Method for fabricating an integral three-dimensional object from layers of a photoformable composition |
| US5266993A (en) * | 1991-10-31 | 1993-11-30 | Nikon Corporation | Focal-plane shutter apparatus |
| JP2012168038A (en) * | 2011-02-15 | 2012-09-06 | Techno Medica Co Ltd | Disposable test plate and analyzer |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU5534194A (en) * | 1992-11-20 | 1994-06-22 | Dainabot Co., Ltd. | Multi-channel automatic immunoassay system |
-
1987
- 1987-07-03 JP JP16551387A patent/JPS6345540A/en active Granted
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0269761U (en) * | 1988-11-15 | 1990-05-28 | ||
| JPH02143166A (en) * | 1988-11-25 | 1990-06-01 | Olympus Optical Co Ltd | Automatic transporting device for biosample |
| US5122441A (en) * | 1990-10-29 | 1992-06-16 | E. I. Du Pont De Nemours And Company | Method for fabricating an integral three-dimensional object from layers of a photoformable composition |
| US5266993A (en) * | 1991-10-31 | 1993-11-30 | Nikon Corporation | Focal-plane shutter apparatus |
| JP2012168038A (en) * | 2011-02-15 | 2012-09-06 | Techno Medica Co Ltd | Disposable test plate and analyzer |
Also Published As
| Publication number | Publication date |
|---|---|
| JPS644143B2 (en) | 1989-01-24 |
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