JPS635005Y2 - - Google Patents
Info
- Publication number
- JPS635005Y2 JPS635005Y2 JP13124582U JP13124582U JPS635005Y2 JP S635005 Y2 JPS635005 Y2 JP S635005Y2 JP 13124582 U JP13124582 U JP 13124582U JP 13124582 U JP13124582 U JP 13124582U JP S635005 Y2 JPS635005 Y2 JP S635005Y2
- Authority
- JP
- Japan
- Prior art keywords
- groove
- gel
- cover plate
- electrically insulating
- capillary
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 239000000758 substrate Substances 0.000 claims description 8
- 238000005192 partition Methods 0.000 claims description 5
- 238000001962 electrophoresis Methods 0.000 claims description 4
- 238000002218 isotachophoresis Methods 0.000 claims description 3
- 150000002500 ions Chemical class 0.000 description 15
- 239000000499 gel Substances 0.000 description 12
- 230000035945 sensitivity Effects 0.000 description 5
- 239000000498 cooling water Substances 0.000 description 4
- 239000012491 analyte Substances 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 239000008151 electrolyte solution Substances 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 238000001649 capillary isotachophoresis Methods 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 239000012777 electrically insulating material Substances 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 229910052697 platinum Inorganic materials 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Landscapes
- Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
- Sampling And Sample Adjustment (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Description
【考案の詳細な説明】
本考案は、ゲルをキヤピラリーに封入した等速
電気泳動分析装置に関する。[Detailed Description of the Invention] The present invention relates to an isotachophoresis analyzer in which a gel is enclosed in a capillary.
従来、イオン物質の分析には細管式等速電気泳
動分析装置が使用されているが、この装置は、キ
ヤピラリーチユーブに溶液を満たすだけなので分
離したイオンの拡散が起こりやすく、シヤープな
境界面を得るためや、感度を高くするためにチユ
ーブを細くするには限界があるといつた問題があ
つた。他方、このような泳動中のイオンの拡散を
防止すべく、ゲルを使用する方法も提案されてい
るが、この方法ではゲルの粘度が高いこともあつ
て、管径を太くしなければならない関係上、各イ
オンのゾーン長が短かくなつて感度が低下すると
いう問題があつた。 Conventionally, a capillary isotachophoresis analyzer has been used to analyze ionic substances, but since this equipment simply fills a capillary tube with a solution, it is easy for separated ions to diffuse, and sharp interfaces are not possible. There was a problem that there was a limit to how thin the tube could be in order to increase the sensitivity or increase the sensitivity. On the other hand, in order to prevent such diffusion of ions during electrophoresis, a method using a gel has been proposed, but this method requires a thick tube diameter due to the high viscosity of the gel. Moreover, there was a problem in that the zone length of each ion became short, resulting in a decrease in sensitivity.
本考案は、このような問題に鑑み、溝を有する
基板と、これを覆うカバープレートとによつて可
及的に細い泳動路を形成し、もつて感度が高く、
しかもシヤープな境界面が得られる簡単な等速電
気泳動分析装置を提供することを目的とする。 In view of these problems, the present invention uses a substrate with grooves and a cover plate that covers the grooves to form an electrophoresis path as narrow as possible, resulting in high sensitivity.
Moreover, it is an object of the present invention to provide a simple isotachophoresis analyzer that can obtain a sharp boundary surface.
そこで、以下に本考案の詳細を図示した実施例
に基づいて説明する。 Therefore, the details of the present invention will be explained below based on illustrated embodiments.
図は本考案の一実施例を示す装置の外観図であ
つて、図中符号１は、プラスチツク等の電気絶縁
性基板２の表面に穿設されたキヤピラリーを形成
する細い溝で、その両端は白金電極３，４によつ
て閉止され、また、この溝１の一方の電極３寄り
には検出器５が、他方の電極４寄りには取りはず
し可能な仕切板６が設けられている。７はガラス
等の電気絶縁性材料によつて形成されたカバープ
レートで、溝１内にアクリルアミドや寒天等の電
気泳動用ゲル８を充填した上でこのカバープレー
ト７を基板２上に被覆して溝１を密閉するように
構成されている。なお、図中符号９は、溝１の下
方に配設した冷却水の通路を示している。 The figure is an external view of a device showing an embodiment of the present invention, and the reference numeral 1 in the figure is a thin groove forming a capillary drilled in the surface of an electrically insulating substrate 2 such as plastic, and both ends of the groove are The groove 1 is closed by platinum electrodes 3 and 4, and a detector 5 is provided near one electrode 3 of the groove 1, and a removable partition plate 6 is provided near the other electrode 4. Reference numeral 7 denotes a cover plate made of an electrically insulating material such as glass, and after filling the groove 1 with an electrophoresis gel 8 such as acrylamide or agar, the cover plate 7 is coated on the substrate 2. It is configured to seal the groove 1. Note that the reference numeral 9 in the figure indicates a cooling water passage provided below the groove 1.
上述した実施例において、まずカバープレート
７を取外し、仕切板６を境にして検出器５側にリ
ーデイング液、つまり分析対象イオンよりも高易
動度の同符号イオンを含む電解液を、またこれと
反対側にターミナル液、つまり低易動度の同符号
イオンを含む電解液を満たす。ついで、仕切板６
を取り外し、その空所に試料を注入し、上からカ
バープレート７をかぶして冷却水通路９に水を流
す。このような準備を終えた段階で電極３，４間
に電源を接続して定電流を流すと、分析対象イオ
ンは、リーデイングイオンとターミナルイオンに
前後をはさまれて泳動が開始される。この際に発
生するジユール熱は、冷却水通路９を流れる水に
より持ち去られゲル８の温度は上昇しない。 In the embodiment described above, first, the cover plate 7 is removed, and a leading liquid, that is, an electrolytic solution containing ions of the same sign with higher mobility than the analyte ions, is placed on the detector 5 side with the partition plate 6 as a boundary. The opposite side is filled with a terminal solution, that is, an electrolytic solution containing ions of the same sign with low mobility. Next, divider plate 6
is removed, a sample is injected into the space, a cover plate 7 is placed on top, and water is allowed to flow into the cooling water passage 9. After completing such preparations, when a power source is connected between the electrodes 3 and 4 and a constant current is applied, the ions to be analyzed are sandwiched between the leading ion and the terminal ion and begin to migrate. The Joule heat generated at this time is carried away by the water flowing through the cooling water passage 9, and the temperature of the gel 8 does not rise.
このようにして分析対象イオンは、ゲルの間を
泳動中にその移動度の違いにより分離され、分離
が完了すると対象イオンは、リーデイングイオン
の後に易動度の大きさの順に配列されて各々がシ
ヤープな界面で接するゾーンを形成しながらゲル
の間を等速度で検出器５に向けて移動し、検出さ
れる。 In this way, the analyte ions are separated based on their mobility while they migrate through the gel, and once the separation is complete, the analyte ions are arranged in order of mobility after the leading ion, and each It moves toward the detector 5 at a constant speed between the gels while forming a zone in contact with a sharp interface, and is detected.
分析を終了した後は、カバープレート７をはず
して溝１内のゲルを箆等で掻出して洗浄した後、
この溝１に新らたなゲルを充填すれば、次の分析
に備えることができる。 After finishing the analysis, remove the cover plate 7 and scrape out the gel in the groove 1 with a spatula or the like and wash it.
By filling this groove 1 with new gel, preparation can be made for the next analysis.
なお、上述した実施例においては、基板２に１
本の溝を設けられているが、複数本の溝を設け
て、これらにゲルを充填し、複数の試料を同時に
分析するようにすることもできる。 In addition, in the above-mentioned embodiment, the substrate 2 has 1
Although a book groove is provided, it is also possible to provide a plurality of grooves and fill them with gel so that a plurality of samples can be analyzed simultaneously.
以上、説明したように本考案によれば、基板の
表面に設けた溝とカバープレートでキヤピラリー
を構成するようにしたので、ゲルの充填、及び除
去が簡単であるばかりでなく、基板上に穿設する
溝の如何によりキヤピラリーの径を細くして可及
的に分離したイオンのゾーン長を大きくして感度
を高めることができ、さらには溝内に仕切板を設
けるようにしたので、リーデイングイオン液とタ
ーミナルイオン液、あるいは試料の注入領域を明
確に画分できて正確な分析を行なわせることがで
きる。 As explained above, according to the present invention, the capillary is constructed from the groove provided on the surface of the substrate and the cover plate, which not only makes it easy to fill and remove gel, but also makes it possible to make holes on the substrate. The diameter of the capillary can be made as narrow as possible to increase the zone length of the separated ions, depending on the type of groove provided, and the sensitivity can be increased.Furthermore, since a partition plate is provided in the groove, the leading ion The liquid and terminal ion liquid or sample injection area can be clearly separated and accurate analysis can be performed.
図は、本考案の一実施例を示す装置の斜視図で
ある。
１……溝、２……基板、３，４……電極、５…
…検出器、６……仕切板、７……カバープレー
ト、８……ゲル、９……冷却水流路。
The figure is a perspective view of an apparatus showing an embodiment of the present invention. 1... Groove, 2... Substrate, 3, 4... Electrode, 5...
...detector, 6 ... partition plate, 7 ... cover plate, 8 ... gel, 9 ... cooling water channel.
Claims (1)
電気絶縁性基板の表面に電気泳動用ゲルの収容溝
を形成するとともに、該溝の両端、一端寄り及び
他端寄りにそれぞれ定電流源に接続する電極、検
出器及び取外し可能な仕切板を配設してなるキヤ
ピラリーゲル等速電気泳動分析装置。 A groove for storing an electrophoresis gel is formed on the surface of an electrically insulating substrate covered with an electrically insulating cover plate, and electrodes are connected to constant current sources at both ends of the groove, near one end, and near the other end. , a capillary gel isotachophoresis analyzer equipped with a detector and a removable partition plate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP13124582U JPS5937557U (en) | 1982-09-01 | 1982-09-01 | Capillary gel isotachophoresis analyzer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP13124582U JPS5937557U (en) | 1982-09-01 | 1982-09-01 | Capillary gel isotachophoresis analyzer |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS5937557U JPS5937557U (en) | 1984-03-09 |
| JPS635005Y2 true JPS635005Y2 (en) | 1988-02-10 |
Family
ID=30296779
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP13124582U Granted JPS5937557U (en) | 1982-09-01 | 1982-09-01 | Capillary gel isotachophoresis analyzer |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS5937557U (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6147549A (en) * | 1984-08-13 | 1986-03-08 | Hitachi Ltd | gel electrophoresis device |
| CN111656179B (en) * | 2017-11-13 | 2023-11-03 | 豪夫迈·罗氏有限公司 | Device for sample analysis using epitope electrophoresis |
-
1982
- 1982-09-01 JP JP13124582U patent/JPS5937557U/en active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| JPS5937557U (en) | 1984-03-09 |
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