JPS63732B2 - - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a method for measuring electrolytes using a flame photometer incorporated into an automatic biochemical analyzer, and more particularly to a calibration method for measuring electrolytes using a flame photometer.

Automated biochemical analyzers, particularly multi-item automatic biochemical analyzers, incorporate a flame photometer, and measure electrolytes in specimens using the flame photometer. When measuring electrolytes in a sample using a flame photometer, calibration (zero point (adjustment, scale inspection, scale calibration, etc.) needed to be performed frequently. Therefore, when a multi-item automatic biochemical analyzer and a flame photometer are connected, conventionally, the operator manually performs calibration operations at will, or manually performs calibration operations each time each sample is measured. was going there. This method has drawbacks such as complicated operations by the operator and too much time required to process multiple samples. In addition, even when using an automatic analyzer for processing multiple samples, calibration is performed simultaneously for colorimetric analysis items and electrolytes, so sufficient drift correction must be performed for electrolyte measurements, which have a larger drift than colorimetric analysis. It had the disadvantage that it could not be used.

When the flame photometer is incorporated into an automatic biochemical analyzer to measure electrolytes in a sample, the present invention automatically calibrates the flame photometer without reducing the sample throughput. The purpose of the present invention is to provide a method for measuring electrolytes that can be carried out.

The present invention incorporates a flame photometer into an automatic biochemical analyzer that automatically analyzes the biochemical components of a specimen stored in a sample cup on a sample cup line and transferred sequentially at a fixed period. When selectively supplying the sample to the analyzer and measuring its electrolyte, if the sample has been ordered for electrolyte measurement, the sample is dispensed into a reaction tube on a reaction tube line that is sequentially transferred at a fixed period. If the sample is not ordered for electrolyte measurement or the sample cup is a calibration cup, the calibration sample is supplied to the reaction tube on the reaction tube line. The present invention is characterized in that the flame photometer is supplied with the flame photometer.

The invention will now be explained in detail with reference to the drawings. The figure is a process diagram showing an outline of an automatic biochemical analyzer incorporating a flame photometer. Reference numeral 1 denotes a sample cup line, which includes a cup into which a sample to be measured is placed. This cup can be used for calibration if no specimen is included. Reference numeral 2 denotes a reaction tube line, which includes reaction tubes into which reagent liquids from reagent tanks 4-A and 4-B are selectively dispensed by reagent liquid dispensers 3-A and 3-B. This reaction tube line 2 and the sample coupling line 1 can correspondingly move at a constant speed in the direction of the arrow. 5 is a sample dispenser, 6 is a flame photometer, and 7 is a stirring device. The sample dispenser 5 and the stirring device 7 are configured to operate when a sample for which an electrolyte measurement is ordered is moved to the sample dispensing position, but not to operate for a sample for which there is no order.

Automatic biochemical analyzer sample coupling line 1
The test solution in the test solution tank 4-A is dispensed by the test solution dispenser 3-A into the reaction tube on the reaction tube line 2 corresponding to the upper calibration cup. The test solution in this test solution tank 4-A is stored in the test solution tank 4-B.
The test solution (internal standard solution) is Na, K standard solution, which is an electrolyte standard solution of known concentration (for example, Na is 145 mEg/
, K is 5mEg/). The dilution ratio of this test solution is the same as the serum dilution ratio. For example, when dispensing 20μ of serum for 2.0ml of reagent in reagent tank 4-B, the serum dilution ratio is 1/101, so the reagent in reagent tank 4-A is the reagent in reagent tank 4-B. 1 volume of Na, K standard solution is added to 100 volumes. Next, the reaction tube into which the reagent in the reagent tank 4-A has been dispensed is moved to the sample dispensing position. However, the sample dispenser 5 does not perform a sample dispensing operation on the calibration cup. Therefore, the reaction tube corresponding to the calibration cup is moved to the measurement position with only the test solution in the test solution tank 4-A contained therein, and the solution is transferred to the flame photometer 6, where the concentration of the solution is measured. . in this case,
The liquid concentration may be measured as a value different from the original concentration due to the drift of the flame photometer. At this time, the concentration of the original electrolyte standard solution/the concentration of the test solution measured with the flame photometer is automatically calculated and used as a correction coefficient, and in subsequent sample measurements, this correction is applied to the value measured with the flame photometer. Multiply by a coefficient and output as the true measured value. For example, in the Na and K standard solutions with the above concentrations, the concentrations of Na and K are 145 mEq/ and 5 mEq/, but if the concentrations of the test solution measured with a flame photometer are measured as 148 mEq/ and 5.4 mEq/, respectively, the correction will be made. The coefficients are 145/148≒0.9797 for Na and 5.0/ for K.
5.4≒0.9259. In subsequent sample measurements, this value will be multiplied by the value measured by the flame photometer and output as the true measured value.

For the reaction tube corresponding to the cup containing the sample to be measured on sample cup line 1,
Unlike the above, the reagent liquid in the reagent tank 4-B is dispensed by the reagent dispenser 3-B. Next, the reaction tube into which the reagent in the reagent tank 4-B has been dispensed is moved to the sample dispensing position, and the sample is dispensed by the sample dispenser 5 from the cup containing the sample to be measured. It is stirred by a stirring device 7. This reaction tube is moved to a measurement position, and the diluted serum, for example, in the reaction tube is transferred to a flame photometer, where the concentration of the liquid is measured. The above-mentioned correction is performed on the measured value by this flame photometer and output.

Furthermore, for a sample for which there is no measurement order, the reagent solution in the reagent tank 4-A is dispensed into the corresponding reaction tube by the reagent dispenser 3-A. However, even if the reaction tube moves to the sample dispensing position, the sample dispenser 5
does not carry out a fractional dispensing operation, so the reaction tube moves to the measurement position and supplies only the reagent in the reagent tank 4-A to the flame photometer. A flame photometer measures this. From this measured value, a correction coefficient is calculated as described above, and subsequent measured values of the measurement specimen with a flame photometer are corrected.

In other embodiments, instead of directly using the correction coefficient calculated from the measurement value of the flame photometer as described above for subsequent corrections, the concentration of the test solution measured with the flame photometer or the correction coefficient calculated from this measurement value may be used. Compare the correction coefficient obtained with the previous measurement value or correction coefficient, and if the difference is smaller than a predetermined percentage or value, use the correction coefficient obtained from the latter measurement value, and if it is larger, use the correction coefficient obtained from the former measurement value. The correction coefficient obtained from the value is used to correct the subsequent sample measurement value. This is performed every time there is a specimen for which there is no measurement order. In addition, when there are multiple consecutive samples without a measurement order, compare the average value of multiple measured values with the previously used measured value or the average value of the measured values, and if the difference between the two is large, the former The correction coefficient calculated from the average value of can be used for subsequent specimen measurements, and if the difference is small, the latter measurement value or the correction coefficient obtained from the average value of the measurement values can be used subsequently. moreover,
In a biochemical analyzer that incorporates not only a flame photometer but also other items, the calibration of the flame photometer and the calibration of other items are performed at the same time, and drift between calibrations is avoided. Also correct.

As described above, according to the method of the present invention, when the sample or sample cup for which electrolyte measurement has not been ordered is a calibration cup, the calibration reagent is supplied to the flame photometer. Calibration of the meter can be performed automatically without reducing sample throughput. Furthermore, even in a biochemical analyzer that incorporates other items in addition to a flame photometer, it is possible to take into account the mutual calibration, so drifts between calibrations can be corrected, and the analysis Accuracy can be increased. Furthermore, since the standard solution with a known concentration was diluted with the internal standard solution for calibration, there would be fluctuations in measurement values due to errors and variations in sampling for calibration, and evaporation of serum for calibration. It is possible to prevent fluctuations in the measured values caused by this, and to perform stable calibration with small day-to-day and day-to-day fluctuations.

In addition, for specimens for which measurement has been ordered, a specimen sample diluted with reagent is supplied to the flame photometer, and for specimens for which measurement has not been ordered or calibration cups, only reagent is supplied to the flame photometer. Since the sample liquid is always supplied to the flame photometer, the temperature of the combustion chamber in the flame photometer, for example, will not rise too much and will maintain a constant temperature. temperature can be maintained.
Even if there are consecutive samples without a measurement order, subsequent sample measurement values can be corrected.
Electrolyte measurements can be performed accurately in a stable state.

This makes it possible to improve the accuracy of an automatic biochemical analyzer incorporating a flame photometer and perform rapid sample processing.

[Brief explanation of the drawing]

The figure is a process diagram schematically showing an automatic biochemical analyzer incorporating a flame photometer according to an embodiment of the present invention. 1... Sample coupling line, 2... Reaction tube line, 3-A, 3-B... Test liquid dispenser, 4-A, 4-
B... Test liquid tank, 5... Sample dispenser, 6... Flame photometer, 7... Stirring device.

Claims (1)

[Claims] 1. A flame photometer is incorporated into an automatic biochemical analyzer that automatically analyzes the biochemical components of a specimen stored in a sample cup on a sample cup line and transferred sequentially at a fixed period. When the sample is selectively supplied to the flame photometer and its electrolyte is measured, if the sample is ordered for electrolyte measurement, the sample is transferred to a reaction tube on the reaction tube line that is sequentially transferred at a fixed period. If the sample is not ordered for electrolyte measurement or the sample cup is a calibration cup, the sample cup is dispensed into the reaction tube on the reaction tube line and supplied to the flame photometer. 1. A method for measuring electrolytes using a flame photometer incorporated into an automatic biochemical analyzer, characterized in that a sample solution for the test is dispensed and supplied to the flame photometer. 2. As the test solution for calibration, an electrolyte standard solution of a known concentration is diluted with the internal standard solution at a dilution ratio that is the same as the dilution ratio at which the sample is diluted with the internal standard solution during electrolyte measurement. The test solution was measured using the flame photometer, and the concentration of the electrolyte standard solution and the concentration of the test solution measured using the flame photometer were determined by the following formula: concentration of electrolyte standard solution/measured using the flame photometer. Claim 1, characterized in that calibration is automatically performed by determining a correction coefficient expressed by the concentration of the test solution = correction coefficient, and multiplying the electrolyte measurement value of each sample by this correction coefficient. An electrolyte measurement method using a flame photometer incorporated into the automatic biochemical analyzer described above. 3 Compare the concentration of the test solution measured with the flame photometer or the correction coefficient obtained from this measurement value with the previous measurement value or correction coefficient, and only if the difference is within a predetermined range, change the correction coefficient to the previous one. An electrolyte measurement method using a flame photometer incorporated in an automatic biochemical analyzer according to claim 2, characterized in that the correction coefficient value is changed to a new current correction coefficient value. 4. The automatic method according to claim 2 or 3, characterized in that when the measurement of the test liquid is performed continuously, a plurality of consecutive constant measured values are calculated and a correction coefficient is determined using the average value. Electrolyte measurement method using a flame photometer built into a biochemical analyzer.