JPS6413986A - Microcapsule for cultivating cell and production thereof - Google Patents
Microcapsule for cultivating cell and production thereofInfo
- Publication number
- JPS6413986A JPS6413986A JP16862487A JP16862487A JPS6413986A JP S6413986 A JPS6413986 A JP S6413986A JP 16862487 A JP16862487 A JP 16862487A JP 16862487 A JP16862487 A JP 16862487A JP S6413986 A JPS6413986 A JP S6413986A
- Authority
- JP
- Japan
- Prior art keywords
- aqueous solution
- cells
- alginate
- cultivating
- polyethyleneimine
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000003094 microcapsule Substances 0.000 title abstract 2
- 239000007864 aqueous solution Substances 0.000 abstract 4
- FHVDTGUDJYJELY-UHFFFAOYSA-N 6-{[2-carboxy-4,5-dihydroxy-6-(phosphanyloxy)oxan-3-yl]oxy}-4,5-dihydroxy-3-phosphanyloxane-2-carboxylic acid Chemical compound O1C(C(O)=O)C(P)C(O)C(O)C1OC1C(C(O)=O)OC(OP)C(O)C1O FHVDTGUDJYJELY-UHFFFAOYSA-N 0.000 abstract 3
- 229940072056 alginate Drugs 0.000 abstract 3
- 235000010443 alginic acid Nutrition 0.000 abstract 3
- 229920000615 alginic acid Polymers 0.000 abstract 3
- 229920002873 Polyethylenimine Polymers 0.000 abstract 2
- 239000012736 aqueous medium Substances 0.000 abstract 2
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 abstract 1
- 230000002378 acidificating effect Effects 0.000 abstract 1
- 239000002775 capsule Substances 0.000 abstract 1
- 239000006143 cell culture medium Substances 0.000 abstract 1
- 239000006285 cell suspension Substances 0.000 abstract 1
- 239000010408 film Substances 0.000 abstract 1
- 239000001963 growth medium Substances 0.000 abstract 1
- 238000002347 injection Methods 0.000 abstract 1
- 239000007924 injection Substances 0.000 abstract 1
- 235000010413 sodium alginate Nutrition 0.000 abstract 1
- 229940005550 sodium alginate Drugs 0.000 abstract 1
- -1 sodium alginate Chemical compound 0.000 abstract 1
- 239000000661 sodium alginate Substances 0.000 abstract 1
- 238000003756 stirring Methods 0.000 abstract 1
- 239000010409 thin film Substances 0.000 abstract 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
PURPOSE:To produce microcapsules for cultivating cells capable of sustaining a cultivation environment in a good state for a long period without damaging cultivated cells, by entrapping an aqueous medium for cultivating the cells in organic thin film capsules. CONSTITUTION:A plant cell suspension and culture medium are added to an aqueous solution of a water-soluble alginate, such as sodium alginate, in a suitable concentration to afford a mixed aqueous solution of the alginate culture medium, containing suspended plant cells and adjusted so as to finally provide about 0.5wt.% alginate concentration. The above-mentioned aqueous solution is then passed through a capillary, such as injection needle, and dripped into an aqueous solution of about 0.5wt.% polyethyleneimine, e.g. kept under acidic condition, while gently stirring to entrap the aqueous medium containing the plant cells in films of the resultant alginic acid-polyethyleneimine adduct.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP16862487A JPS6413986A (en) | 1987-07-08 | 1987-07-08 | Microcapsule for cultivating cell and production thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP16862487A JPS6413986A (en) | 1987-07-08 | 1987-07-08 | Microcapsule for cultivating cell and production thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPS6413986A true JPS6413986A (en) | 1989-01-18 |
Family
ID=15871507
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP16862487A Pending JPS6413986A (en) | 1987-07-08 | 1987-07-08 | Microcapsule for cultivating cell and production thereof |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS6413986A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101803401A (en) * | 2008-06-16 | 2010-08-11 | 株式会社特瑞君思半导体 | Digital Speaker Driver |
| EP3702442A1 (en) * | 2011-12-22 | 2020-09-02 | Life Technologies Corporation | Cell culture media and methods |
-
1987
- 1987-07-08 JP JP16862487A patent/JPS6413986A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101803401A (en) * | 2008-06-16 | 2010-08-11 | 株式会社特瑞君思半导体 | Digital Speaker Driver |
| EP3702442A1 (en) * | 2011-12-22 | 2020-09-02 | Life Technologies Corporation | Cell culture media and methods |
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