JPS6416581A - Frozen animal cell and production thereof - Google Patents

Frozen animal cell and production thereof

Info

Publication number
JPS6416581A
JPS6416581A JP62171772A JP17177287A JPS6416581A JP S6416581 A JPS6416581 A JP S6416581A JP 62171772 A JP62171772 A JP 62171772A JP 17177287 A JP17177287 A JP 17177287A JP S6416581 A JPS6416581 A JP S6416581A
Authority
JP
Japan
Prior art keywords
cell
culture
freezing
substrate
medium
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP62171772A
Other languages
Japanese (ja)
Other versions
JPH0577389B2 (en
Inventor
Tadao Ono
Shiyou Kurita
Naoko Emori
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
RIKEN
Original Assignee
RIKEN
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by RIKEN filed Critical RIKEN
Priority to JP62171772A priority Critical patent/JPS6416581A/en
Publication of JPS6416581A publication Critical patent/JPS6416581A/en
Publication of JPH0577389B2 publication Critical patent/JPH0577389B2/ja
Granted legal-status Critical Current

Links

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  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

PURPOSE:To obtain the titled cell preservable over a long period, without causing the loss of culture tools accompanied by the dissemination of cells after freezing and thawing, by culturing an anchorage-dependent cell on a culture substrate, exchanging the culture medium with a freezing medium and immediately freezing the cell together with the substrate. CONSTITUTION:An anchorage-dependent cell is cultured on a culture substrate, the culture medium is exchanged to a freezing medium and the cell is immediately frozen together with the culture substrate to obtain the objective cell. Preferably, the culture of the cell is carried out until the cell density reaches a confluent state and the surface of the culture substrate is coated with an adhesion- promoting substance (e.g. gelatin) prior to the dissemination of the cell.
JP62171772A 1987-07-09 1987-07-09 Frozen animal cell and production thereof Granted JPS6416581A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP62171772A JPS6416581A (en) 1987-07-09 1987-07-09 Frozen animal cell and production thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP62171772A JPS6416581A (en) 1987-07-09 1987-07-09 Frozen animal cell and production thereof

Publications (2)

Publication Number Publication Date
JPS6416581A true JPS6416581A (en) 1989-01-20
JPH0577389B2 JPH0577389B2 (en) 1993-10-26

Family

ID=15929394

Family Applications (1)

Application Number Title Priority Date Filing Date
JP62171772A Granted JPS6416581A (en) 1987-07-09 1987-07-09 Frozen animal cell and production thereof

Country Status (1)

Country Link
JP (1) JPS6416581A (en)

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0433436U (en) * 1990-07-17 1992-03-18
JP2008212062A (en) * 2007-03-05 2008-09-18 Niwa Masami Cell cryopreservation method and cell culture method
WO2009050965A1 (en) * 2007-10-19 2009-04-23 Japan As Represented By General Director Of Agency Of National Center For Child Health And Development Support for immobilizing frozen cells, tool for primary hepatocyte culture and method of producing tool for primary hepatocyte culture
US8742091B2 (en) 2001-06-20 2014-06-03 Dainippon Sumitomo Pharma Co., Ltd. Method of promoting nucleic acid transfer
JP2015500810A (en) * 2011-11-30 2015-01-08 アドバンスド セル テクノロジー、インコーポレイテッド Mesenchymal stromal cells and related applications
US12209255B2 (en) 2012-07-12 2025-01-28 Astellas Institute For Regenerative Medicine Mesenchymal-like stem cells derived from human embryonic stem cells, methods and uses thereof
US12465621B2 (en) 2011-11-30 2025-11-11 Astellas Institute For Regenerative Medicine Mesenchymal stromal cells and uses related thereto

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2018310000B2 (en) * 2017-07-31 2021-08-05 Two Cells Co., Ltd. Composition for cryopreservation, method for producing cryopreserved material, cell preparation, method for producing cell preparation, and kit for cryopreservation
WO2020067434A1 (en) * 2018-09-27 2020-04-02 テルモ株式会社 Method for cryopreserving cells

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0433436U (en) * 1990-07-17 1992-03-18
US8742091B2 (en) 2001-06-20 2014-06-03 Dainippon Sumitomo Pharma Co., Ltd. Method of promoting nucleic acid transfer
JP2008212062A (en) * 2007-03-05 2008-09-18 Niwa Masami Cell cryopreservation method and cell culture method
WO2009050965A1 (en) * 2007-10-19 2009-04-23 Japan As Represented By General Director Of Agency Of National Center For Child Health And Development Support for immobilizing frozen cells, tool for primary hepatocyte culture and method of producing tool for primary hepatocyte culture
JP2015500810A (en) * 2011-11-30 2015-01-08 アドバンスド セル テクノロジー、インコーポレイテッド Mesenchymal stromal cells and related applications
US12097223B2 (en) 2011-11-30 2024-09-24 Astellas Institute For Regenerative Medicine Mesenchymal stromal cells and uses related thereto
US12465621B2 (en) 2011-11-30 2025-11-11 Astellas Institute For Regenerative Medicine Mesenchymal stromal cells and uses related thereto
US12209255B2 (en) 2012-07-12 2025-01-28 Astellas Institute For Regenerative Medicine Mesenchymal-like stem cells derived from human embryonic stem cells, methods and uses thereof

Also Published As

Publication number Publication date
JPH0577389B2 (en) 1993-10-26

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