JPS6420099A - Method for determining free fatty acid and reagent composition for determining free fatty acid useful in said determination - Google Patents
Method for determining free fatty acid and reagent composition for determining free fatty acid useful in said determinationInfo
- Publication number
- JPS6420099A JPS6420099A JP17492787A JP17492787A JPS6420099A JP S6420099 A JPS6420099 A JP S6420099A JP 17492787 A JP17492787 A JP 17492787A JP 17492787 A JP17492787 A JP 17492787A JP S6420099 A JPS6420099 A JP S6420099A
- Authority
- JP
- Japan
- Prior art keywords
- fatty acid
- free fatty
- determining free
- acylcoenzyme
- determination
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 235000021588 free fatty acids Nutrition 0.000 title abstract 4
- 239000003153 chemical reaction reagent Substances 0.000 title 1
- 102000004539 Acyl-CoA Oxidase Human genes 0.000 abstract 2
- 108020001558 Acyl-CoA oxidase Proteins 0.000 abstract 2
- RGJOEKWQDUBAIZ-IBOSZNHHSA-N CoASH Chemical compound O[C@@H]1[C@H](OP(O)(O)=O)[C@@H](COP(O)(=O)OP(O)(=O)OCC(C)(C)[C@@H](O)C(=O)NCCC(=O)NCCS)O[C@H]1N1C2=NC=NC(N)=C2N=C1 RGJOEKWQDUBAIZ-IBOSZNHHSA-N 0.000 abstract 2
- RGJOEKWQDUBAIZ-UHFFFAOYSA-N coenzime A Natural products OC1C(OP(O)(O)=O)C(COP(O)(=O)OP(O)(=O)OCC(C)(C)C(O)C(=O)NCCC(=O)NCCS)OC1N1C2=NC=NC(N)=C2N=C1 RGJOEKWQDUBAIZ-UHFFFAOYSA-N 0.000 abstract 2
- 239000005516 coenzyme A Substances 0.000 abstract 2
- 229940093530 coenzyme a Drugs 0.000 abstract 2
- KDTSHFARGAKYJN-UHFFFAOYSA-N dephosphocoenzyme A Natural products OC1C(O)C(COP(O)(=O)OP(O)(=O)OCC(C)(C)C(O)C(=O)NCCC(=O)NCCS)OC1N1C2=NC=NC(N)=C2N=C1 KDTSHFARGAKYJN-UHFFFAOYSA-N 0.000 abstract 2
- 125000005480 straight-chain fatty acid group Chemical group 0.000 abstract 2
- LNQVTSROQXJCDD-KQYNXXCUSA-N 3'-AMP Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](CO)[C@@H](OP(O)(O)=O)[C@H]1O LNQVTSROQXJCDD-KQYNXXCUSA-N 0.000 abstract 1
- 102000004190 Enzymes Human genes 0.000 abstract 1
- 108090000790 Enzymes Proteins 0.000 abstract 1
- 102000003992 Peroxidases Human genes 0.000 abstract 1
- 108040007629 peroxidase activity proteins Proteins 0.000 abstract 1
- 239000000049 pigment Substances 0.000 abstract 1
- 230000035945 sensitivity Effects 0.000 abstract 1
- 239000000126 substance Substances 0.000 abstract 1
Landscapes
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
PURPOSE:To measure a free fatty acid in high precise and sensitivity, by reacting a substance other than straight-chain fatty acid with coenzyme A and acylcoenzyme A oxidase and measuring the change after the reaction. CONSTITUTION:A free fatty acid other than a straight-chain fatty acid is reacted with an acylcoenzyme A synthetic enzyme in the presence of adenosine 3-phosphoric acid and coenzyme A to provide an acylcoenzyme A, which is reacted with an acylcoenzyme A oxidase at pH6.0-8.5 to oxidize the acylcoenzyme A and peroxidase, takarase, etc., is added to the resultant H2O2 to provide a pigment, which is then subjected to colorimetric determination.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP17492787A JP2704616B2 (en) | 1987-07-15 | 1987-07-15 | Free fatty acid determination method and reagent composition for free fatty acid determination useful for the determination method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP17492787A JP2704616B2 (en) | 1987-07-15 | 1987-07-15 | Free fatty acid determination method and reagent composition for free fatty acid determination useful for the determination method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS6420099A true JPS6420099A (en) | 1989-01-24 |
| JP2704616B2 JP2704616B2 (en) | 1998-01-26 |
Family
ID=15987151
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP17492787A Expired - Lifetime JP2704616B2 (en) | 1987-07-15 | 1987-07-15 | Free fatty acid determination method and reagent composition for free fatty acid determination useful for the determination method |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP2704616B2 (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0771760A2 (en) | 1995-11-02 | 1997-05-07 | Fuji Photo Film Co., Ltd. | Process for the purification of aluminum hydroxide and process for the surface treatment of aluminum plate utilizing the purification process |
| US6320124B1 (en) * | 1999-10-26 | 2001-11-20 | Veterans General Hospital - Taipei | Electrophysiological faraday box |
| JP2002051769A (en) * | 2000-08-11 | 2002-02-19 | Azwell Inc | Liquid reagent useful for measuring nonesterified fatty acid |
-
1987
- 1987-07-15 JP JP17492787A patent/JP2704616B2/en not_active Expired - Lifetime
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0771760A2 (en) | 1995-11-02 | 1997-05-07 | Fuji Photo Film Co., Ltd. | Process for the purification of aluminum hydroxide and process for the surface treatment of aluminum plate utilizing the purification process |
| US6320124B1 (en) * | 1999-10-26 | 2001-11-20 | Veterans General Hospital - Taipei | Electrophysiological faraday box |
| JP2002051769A (en) * | 2000-08-11 | 2002-02-19 | Azwell Inc | Liquid reagent useful for measuring nonesterified fatty acid |
Also Published As
| Publication number | Publication date |
|---|---|
| JP2704616B2 (en) | 1998-01-26 |
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