JPS6429765A - Diagnosing device for number and activity of cells - Google Patents
Diagnosing device for number and activity of cellsInfo
- Publication number
- JPS6429765A JPS6429765A JP18536887A JP18536887A JPS6429765A JP S6429765 A JPS6429765 A JP S6429765A JP 18536887 A JP18536887 A JP 18536887A JP 18536887 A JP18536887 A JP 18536887A JP S6429765 A JPS6429765 A JP S6429765A
- Authority
- JP
- Japan
- Prior art keywords
- cells
- image
- cell
- activity
- luminance
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000007788 liquid Substances 0.000 abstract 3
- 238000004043 dyeing Methods 0.000 abstract 2
- 238000006243 chemical reaction Methods 0.000 abstract 1
- 238000002347 injection Methods 0.000 abstract 1
- 239000007924 injection Substances 0.000 abstract 1
- 239000006194 liquid suspension Substances 0.000 abstract 1
- 244000005700 microbiome Species 0.000 abstract 1
- 239000000203 mixture Substances 0.000 abstract 1
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
Abstract
PURPOSE:To enable the counting of cells including the activity thereof and whether the cells are live or dead and to facilitate the recognition of the cells by injection of a dyeing liquid by updating the magnified image of the cells or microorganisms in a medium, then subjecting the difference in the luminance of each cell of every one image screen to image processing. CONSTITUTION:A liquid suspension 11 contg. the cells in a culture vessel 10 is sampled 20 and is mixed 23 with the dyeing liquid 31. The liquid mixture is transported to a flow cell 41. The image of the cells is magnified by an image magnifying device 42 in the cell 41 and since the cell 41 is moved in directions X and Y by an X-Y stage 42, the image is successively updated. The images are picked up by a TV camera 44, are subjected to A/D conversion 51 and are stored in an image memory 51M. The many-valued images stored therein are sent to a live cell image recognizing deice 60 where the number, size, shape, and luminance, etc., of all the cells are calculated. The images are also sent to a dead cell image recognizing device 70 where the calculations are similarly made with the dead cells and low-activity cells. The respective results of the calculations are sent to an activity diagnosing device 80 where the number, size, shape and luminance, etc., of the cells are calculated. The active condition of the cell groups is diagnosed overall from the results thereof.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP62185368A JPH0782008B2 (en) | 1987-07-27 | 1987-07-27 | Device for diagnosing cell number and activity |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP62185368A JPH0782008B2 (en) | 1987-07-27 | 1987-07-27 | Device for diagnosing cell number and activity |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS6429765A true JPS6429765A (en) | 1989-01-31 |
| JPH0782008B2 JPH0782008B2 (en) | 1995-09-06 |
Family
ID=16169578
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP62185368A Expired - Lifetime JPH0782008B2 (en) | 1987-07-27 | 1987-07-27 | Device for diagnosing cell number and activity |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH0782008B2 (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0489595A (en) * | 1990-07-31 | 1992-03-23 | Ntn Corp | Controlling circuit of x-y stage |
| JP2013517460A (en) * | 2010-01-12 | 2013-05-16 | バイオ−ラド ラボラトリーズ インコーポレイテッド | Cell characterization using multiple focal planes |
| JP2014161266A (en) * | 2013-02-25 | 2014-09-08 | Dainippon Printing Co Ltd | Culture bag |
| WO2015004917A1 (en) * | 2013-07-10 | 2015-01-15 | 株式会社ニコン | Observation method, observation device, method for producing cell sheet and device for producing cell sheet |
| JP2016116461A (en) * | 2014-12-19 | 2016-06-30 | パナソニック株式会社 | Cell determination method, and cell number measurement method |
| JPWO2022186237A1 (en) * | 2021-03-03 | 2022-09-09 |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2026017218A (en) | 2024-07-23 | 2026-02-04 | 富士フイルム株式会社 | Particle detection device and particle detection method |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5250558A (en) * | 1975-10-20 | 1977-04-22 | Mitsubishi Marorii Yakin Kougi | Silverrnickellmetallic oxide electric contacts |
| JPS5698655A (en) * | 1980-01-11 | 1981-08-08 | Omron Tateisi Electronics Co | Cell analyzer |
| JPS5752859A (en) * | 1980-09-16 | 1982-03-29 | Omron Tateisi Electronics Co | Cell analyzer |
| JPS6031887A (en) * | 1983-07-28 | 1985-02-18 | Hitachi Ltd | Microorganism phase discriminating apparatus |
| JPS61247962A (en) * | 1985-04-26 | 1986-11-05 | Hamamatsu Photonics Kk | Cell diagnosing apparatus |
| JPS6253791A (en) * | 1985-08-30 | 1987-03-09 | Hitachi Ltd | Apparatus for detecting microorganism |
| JPS63282653A (en) * | 1987-05-14 | 1988-11-18 | Kachiku Jiyuseiran Ishiyoku Gijutsu Kenkyu Kumiai | Method for measuring activity of cell |
-
1987
- 1987-07-27 JP JP62185368A patent/JPH0782008B2/en not_active Expired - Lifetime
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5250558A (en) * | 1975-10-20 | 1977-04-22 | Mitsubishi Marorii Yakin Kougi | Silverrnickellmetallic oxide electric contacts |
| JPS5698655A (en) * | 1980-01-11 | 1981-08-08 | Omron Tateisi Electronics Co | Cell analyzer |
| JPS5752859A (en) * | 1980-09-16 | 1982-03-29 | Omron Tateisi Electronics Co | Cell analyzer |
| JPS6031887A (en) * | 1983-07-28 | 1985-02-18 | Hitachi Ltd | Microorganism phase discriminating apparatus |
| JPS61247962A (en) * | 1985-04-26 | 1986-11-05 | Hamamatsu Photonics Kk | Cell diagnosing apparatus |
| JPS6253791A (en) * | 1985-08-30 | 1987-03-09 | Hitachi Ltd | Apparatus for detecting microorganism |
| JPS63282653A (en) * | 1987-05-14 | 1988-11-18 | Kachiku Jiyuseiran Ishiyoku Gijutsu Kenkyu Kumiai | Method for measuring activity of cell |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0489595A (en) * | 1990-07-31 | 1992-03-23 | Ntn Corp | Controlling circuit of x-y stage |
| JP2013517460A (en) * | 2010-01-12 | 2013-05-16 | バイオ−ラド ラボラトリーズ インコーポレイテッド | Cell characterization using multiple focal planes |
| US9001200B2 (en) | 2010-01-12 | 2015-04-07 | Bio-Rad Laboratories, Inc. | Cell characterization using multiple focus planes |
| JP2014161266A (en) * | 2013-02-25 | 2014-09-08 | Dainippon Printing Co Ltd | Culture bag |
| WO2015004917A1 (en) * | 2013-07-10 | 2015-01-15 | 株式会社ニコン | Observation method, observation device, method for producing cell sheet and device for producing cell sheet |
| JPWO2015004917A1 (en) * | 2013-07-10 | 2017-03-02 | 株式会社ニコン | Observation method, cell sheet manufacturing method, cell sheet manufacturing apparatus, and cell sheet observation apparatus |
| JP2016116461A (en) * | 2014-12-19 | 2016-06-30 | パナソニック株式会社 | Cell determination method, and cell number measurement method |
| JPWO2022186237A1 (en) * | 2021-03-03 | 2022-09-09 | ||
| WO2022186237A1 (en) * | 2021-03-03 | 2022-09-09 | テルモ株式会社 | Sampling device and cell culture system |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0782008B2 (en) | 1995-09-06 |
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