JPS643552A - Enzyme sensor - Google Patents
Enzyme sensorInfo
- Publication number
- JPS643552A JPS643552A JP62157851A JP15785187A JPS643552A JP S643552 A JPS643552 A JP S643552A JP 62157851 A JP62157851 A JP 62157851A JP 15785187 A JP15785187 A JP 15785187A JP S643552 A JPS643552 A JP S643552A
- Authority
- JP
- Japan
- Prior art keywords
- electrode
- film
- oxygen reduction
- cobalt
- base body
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 108090000790 Enzymes Proteins 0.000 title abstract 3
- 102000004190 Enzymes Human genes 0.000 title abstract 3
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 abstract 5
- 229910052760 oxygen Inorganic materials 0.000 abstract 5
- 239000001301 oxygen Substances 0.000 abstract 5
- 229910017052 cobalt Inorganic materials 0.000 abstract 3
- 239000010941 cobalt Substances 0.000 abstract 3
- GUTLYIVDDKVIGB-UHFFFAOYSA-N cobalt atom Chemical compound [Co] GUTLYIVDDKVIGB-UHFFFAOYSA-N 0.000 abstract 3
- 239000000853 adhesive Substances 0.000 abstract 2
- 239000003054 catalyst Substances 0.000 abstract 2
- 239000011248 coating agent Substances 0.000 abstract 2
- 238000000576 coating method Methods 0.000 abstract 2
- 229940088598 enzyme Drugs 0.000 abstract 2
- 238000005259 measurement Methods 0.000 abstract 2
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 abstract 2
- 150000004032 porphyrins Chemical class 0.000 abstract 2
- 230000035945 sensitivity Effects 0.000 abstract 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 abstract 1
- 229920000049 Carbon (fiber) Polymers 0.000 abstract 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 abstract 1
- 108010015776 Glucose oxidase Proteins 0.000 abstract 1
- 239000004366 Glucose oxidase Substances 0.000 abstract 1
- 229920006362 Teflon® Polymers 0.000 abstract 1
- 239000004917 carbon fiber Substances 0.000 abstract 1
- 238000006911 enzymatic reaction Methods 0.000 abstract 1
- 229920006332 epoxy adhesive Polymers 0.000 abstract 1
- 239000008103 glucose Substances 0.000 abstract 1
- 229940116332 glucose oxidase Drugs 0.000 abstract 1
- 235000019420 glucose oxidase Nutrition 0.000 abstract 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 abstract 1
- 239000010931 gold Substances 0.000 abstract 1
- 229910052737 gold Inorganic materials 0.000 abstract 1
- 229910002804 graphite Inorganic materials 0.000 abstract 1
- 239000010439 graphite Substances 0.000 abstract 1
- WABPQHHGFIMREM-UHFFFAOYSA-N lead(0) Chemical compound [Pb] WABPQHHGFIMREM-UHFFFAOYSA-N 0.000 abstract 1
- 239000000463 material Substances 0.000 abstract 1
- 229910052751 metal Inorganic materials 0.000 abstract 1
- 239000002184 metal Substances 0.000 abstract 1
- 150000002739 metals Chemical class 0.000 abstract 1
- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical compound C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 abstract 1
- IEQIEDJGQAUEQZ-UHFFFAOYSA-N phthalocyanine Chemical compound N1C(N=C2C3=CC=CC=C3C(N=C3C4=CC=CC=C4C(=N4)N3)=N2)=C(C=CC=C2)C2=C1N=C1C2=CC=CC=C2C4=N1 IEQIEDJGQAUEQZ-UHFFFAOYSA-N 0.000 abstract 1
- 229910052697 platinum Inorganic materials 0.000 abstract 1
- 229920006254 polymer film Polymers 0.000 abstract 1
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Abstract
PURPOSE:To improve measurement sensitivity by coating a conductive base body, except a part thereof, with an oxygen reduction catalyst layer, coating the remaining electrode part with an enzyme layer and measuring the oxygen concn. consumed by the enzyme reaction by oxygen reduction current. CONSTITUTION:The conductive base body 1 is formed by adhering a lead wire 3 by means of a conductive adhesive agent 2 to the terminal of, for example, a carbon fiber CF and insulating the circumference with an epoxy adhesive agent 4 and a 'Teflon(R)' tube 5 to constitute the CF electrode in such a manner that only the section of the CF 1 serves as an electrode surface. The material for the base body 1 is selected from graphite or metals such as platinum and gold in addition to CF. A 3-electrode cell is used for the CF electrode and an electrolytically polymerized film 6 of, for example, cobalt/porphyrin is deposited thereon. The polymer film consisting of a cobalt/phthalocyanine complex or cobalt/porphyrin complex, etc., is selected for the film 6 to form the oxygen reduction catalyst layer having an oxygen reduction function. An enzyme film 7, for example, glucose oxidase film is then deposited by using the 3-electrode cell to prepare a glucose sensor 10. The high measurement sensitivity is thus obtd.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP62157851A JPS643552A (en) | 1987-06-26 | 1987-06-26 | Enzyme sensor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP62157851A JPS643552A (en) | 1987-06-26 | 1987-06-26 | Enzyme sensor |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS643552A true JPS643552A (en) | 1989-01-09 |
| JPH0557538B2 JPH0557538B2 (en) | 1993-08-24 |
Family
ID=15658756
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP62157851A Granted JPS643552A (en) | 1987-06-26 | 1987-06-26 | Enzyme sensor |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS643552A (en) |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH02231558A (en) * | 1989-03-03 | 1990-09-13 | Terumo Corp | Enzyme sensor |
| JPH02253149A (en) * | 1989-03-28 | 1990-10-11 | Terumo Corp | Enzyme sensor and production thereof |
| CN102528626A (en) * | 2012-03-14 | 2012-07-04 | 胡达广 | Metal Cutter with Dual Rotating Baffles |
| US10278632B2 (en) | 2008-11-14 | 2019-05-07 | Pepex Biomedical, LLC. | Electrochemical sensor module |
| US11016052B2 (en) | 2007-08-30 | 2021-05-25 | Pepex Biomedical Inc. | Electrochemical sensor and method for manufacturing |
| US11045124B2 (en) | 2014-06-04 | 2021-06-29 | Pepex Biomedical, Inc. | Electrochemical sensors and methods for making electrochemical sensors using advanced printing technology |
| US11150212B2 (en) | 2007-08-30 | 2021-10-19 | Pepex Biomedical, Inc. | Electrochemical sensor and method for manufacturing |
| US11224367B2 (en) | 2012-12-03 | 2022-01-18 | Pepex Biomedical, Inc. | Sensor module and method of using a sensor module |
-
1987
- 1987-06-26 JP JP62157851A patent/JPS643552A/en active Granted
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH02231558A (en) * | 1989-03-03 | 1990-09-13 | Terumo Corp | Enzyme sensor |
| JPH02253149A (en) * | 1989-03-28 | 1990-10-11 | Terumo Corp | Enzyme sensor and production thereof |
| US11016052B2 (en) | 2007-08-30 | 2021-05-25 | Pepex Biomedical Inc. | Electrochemical sensor and method for manufacturing |
| US11150212B2 (en) | 2007-08-30 | 2021-10-19 | Pepex Biomedical, Inc. | Electrochemical sensor and method for manufacturing |
| US10278632B2 (en) | 2008-11-14 | 2019-05-07 | Pepex Biomedical, LLC. | Electrochemical sensor module |
| CN102528626A (en) * | 2012-03-14 | 2012-07-04 | 胡达广 | Metal Cutter with Dual Rotating Baffles |
| US11224367B2 (en) | 2012-12-03 | 2022-01-18 | Pepex Biomedical, Inc. | Sensor module and method of using a sensor module |
| US11045124B2 (en) | 2014-06-04 | 2021-06-29 | Pepex Biomedical, Inc. | Electrochemical sensors and methods for making electrochemical sensors using advanced printing technology |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0557538B2 (en) | 1993-08-24 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| LAPS | Cancellation because of no payment of annual fees |