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Nippon Kayaku Co Ltd
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Nippon Kayaku Co Ltd
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Application filed by Nippon Kayaku Co LtdfiledCriticalNippon Kayaku Co Ltd
Priority to JP62195809ApriorityCriticalpatent/JPS6439992A/en
Publication of JPS6439992ApublicationCriticalpatent/JPS6439992A/en
Preparation Of Compounds By Using Micro-Organisms
(AREA)
Micro-Organisms Or Cultivation Processes Thereof
(AREA)
Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents
(AREA)
Abstract
PURPOSE:To obtain a monoclonal antibody to be specifically reacted with human osteosarcoma useful with respect to medicine and clinical diagnosis, by cloning an immunocyte of mouse. CONSTITUTION:An animal such as mouse is immunized against an antigen (e.g. isolated antigen, antigen-containing tumor cell or homogenate thereof) to be recognized by a monoclonal antibody and an antibody-forming cell is obtained from an immunized animal. Then the cell is fused with a myeloma cell to give a fused cell, which is screened to give a fused cell to produce a monoclonal antibody. Then the fused cell is cloned and cultivated in a medium to give a supernatant liquid of the culture. The supernatant liquid is separated and purified to give a monoclonal antibody such as mouse monoclonal antibody 2H10 or 2D3 to be specifically reacted with human osteosarcoma.
Antibodies (including chimeric antibodies) to a human tumour antigen, polynucleotides encoding them, detection of the antigen by immunoassay and imaging methods and also cell killing methods
Monoclonal antibody to human T cells, method for preparing it, hybrid cell line producing it, therapeutic composition comprising it and diagnostic method using it.
HYBRID CELL LINE FOR MANUFACTURE OF COMPLEMENTARY FIXING MONOCLONAL ANTIBODY FOR HUMAN REPRESSED T-CELLS, ANTIBODY AND PROCEDURES FOR ITS MANUFACTURING