JPS6439997A - Production of l-tryptophan by fermentation - Google Patents
Production of l-tryptophan by fermentationInfo
- Publication number
- JPS6439997A JPS6439997A JP19848287A JP19848287A JPS6439997A JP S6439997 A JPS6439997 A JP S6439997A JP 19848287 A JP19848287 A JP 19848287A JP 19848287 A JP19848287 A JP 19848287A JP S6439997 A JPS6439997 A JP S6439997A
- Authority
- JP
- Japan
- Prior art keywords
- tryptophan
- strain
- culture medium
- citrobacter
- cultivated
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 title abstract 9
- 230000004151 fermentation Effects 0.000 title 1
- 238000000855 fermentation Methods 0.000 title 1
- 238000004519 manufacturing process Methods 0.000 title 1
- 229960004799 tryptophan Drugs 0.000 abstract 5
- 241000588923 Citrobacter Species 0.000 abstract 3
- 239000001963 growth medium Substances 0.000 abstract 3
- QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 abstract 2
- 239000005557 antagonist Substances 0.000 abstract 2
- 238000009630 liquid culture Methods 0.000 abstract 2
- 230000004060 metabolic process Effects 0.000 abstract 2
- NWUYHJFMYQTDRP-UHFFFAOYSA-N 1,2-bis(ethenyl)benzene;1-ethenyl-2-ethylbenzene;styrene Chemical compound C=CC1=CC=CC=C1.CCC1=CC=CC=C1C=C.C=CC1=CC=CC=C1C=C NWUYHJFMYQTDRP-UHFFFAOYSA-N 0.000 abstract 1
- 241000894006 Bacteria Species 0.000 abstract 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 abstract 1
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 abstract 1
- 230000002378 acidificating effect Effects 0.000 abstract 1
- 239000003674 animal food additive Substances 0.000 abstract 1
- 239000004202 carbamide Substances 0.000 abstract 1
- 239000003729 cation exchange resin Substances 0.000 abstract 1
- 239000003814 drug Substances 0.000 abstract 1
- 230000001747 exhibiting effect Effects 0.000 abstract 1
- 239000000706 filtrate Substances 0.000 abstract 1
- 239000008103 glucose Substances 0.000 abstract 1
- 239000002609 medium Substances 0.000 abstract 1
- 244000005700 microbiome Species 0.000 abstract 1
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
PURPOSE:To obtain L-tryptophan useful for a medicine, feed additive, etc., in high accumulating concentration at a low cost on an industrial scale, by aerobically cultivating a microorganism belonging to the genus Citrobacter and having L-tryptophan producing ability in a liquid culture medium. CONSTITUTION:Citrobacter freudii IFO 13545 strain is subjected to variation treatment by ultraviolet ray irradiation, etc., to provide Citrobacter freudii FTR 66-36 strain exhibiting >=50% in relative growth degree when cultivated in a tryptophan metabolism antagonist-containing medium prepared as to be <=40% in relative growth rate after 24hr in cultivation of parent strain and being a variant strain resistant to tryptophan metabolism antagonist. The variant strain is aerobically cultivated in a liquid culture medium containing 2-15wt.% glucose, 0.5-4wt.% urea at 5-9pH and 24-37 deg.C for 48-120hr. Then the filtrate obtained by removing a bacterium cell from the resultant culture medium is passed through a strongly acidic cation exchange resin, etc., eluted, concentrated and purified to provide the aimed L-tryptophan.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP19848287A JPS6439997A (en) | 1987-08-07 | 1987-08-07 | Production of l-tryptophan by fermentation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP19848287A JPS6439997A (en) | 1987-08-07 | 1987-08-07 | Production of l-tryptophan by fermentation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPS6439997A true JPS6439997A (en) | 1989-02-10 |
Family
ID=16391846
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP19848287A Pending JPS6439997A (en) | 1987-08-07 | 1987-08-07 | Production of l-tryptophan by fermentation |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS6439997A (en) |
-
1987
- 1987-08-07 JP JP19848287A patent/JPS6439997A/en active Pending
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