JPS6439999A - Stabilization of myc protein, variant myc gene, recombinant plasmid containing said gene and production of variant myc protein using transformant by said recombinant plasmid - Google Patents
Stabilization of myc protein, variant myc gene, recombinant plasmid containing said gene and production of variant myc protein using transformant by said recombinant plasmidInfo
- Publication number
- JPS6439999A JPS6439999A JP19719787A JP19719787A JPS6439999A JP S6439999 A JPS6439999 A JP S6439999A JP 19719787 A JP19719787 A JP 19719787A JP 19719787 A JP19719787 A JP 19719787A JP S6439999 A JPS6439999 A JP S6439999A
- Authority
- JP
- Japan
- Prior art keywords
- myc
- variant
- gene
- myc protein
- recombinant plasmid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Abstract
PURPOSE:To readily and stably obtain the titled variant myc protein useful in remedy, diagnosis, etc., for cancer, by cultivating a transformant obtained by introducing a plasmid containing a specific myc gene to a host cell in a culture medium. CONSTITUTION:A human placenta cDNA library, etc., is selected to afford C-myc cDNA coding myc protein which is a human carcinogenic gene product. Then cDNA codon coding a constitutional amino acid of the C-myc protein is converted into other codon coding one or more amino acid for obtaining stable myc protein by site-specific mutagenesis method to give a variant myc gene (A) having a base sequence expressed by the formula. Then a vector for express of the component A is constituted to afford a recombinant plasmid (B). Further transformant obtained by introducing the component B into a human cell, etc., is cultivated in a culture medium and the resultant cultivation product is separated and purified according to Rosenberg method to provide the variant myc protein.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP19719787A JPS6439999A (en) | 1987-08-06 | 1987-08-06 | Stabilization of myc protein, variant myc gene, recombinant plasmid containing said gene and production of variant myc protein using transformant by said recombinant plasmid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP19719787A JPS6439999A (en) | 1987-08-06 | 1987-08-06 | Stabilization of myc protein, variant myc gene, recombinant plasmid containing said gene and production of variant myc protein using transformant by said recombinant plasmid |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPS6439999A true JPS6439999A (en) | 1989-02-10 |
Family
ID=16370426
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP19719787A Pending JPS6439999A (en) | 1987-08-06 | 1987-08-06 | Stabilization of myc protein, variant myc gene, recombinant plasmid containing said gene and production of variant myc protein using transformant by said recombinant plasmid |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS6439999A (en) |
-
1987
- 1987-08-06 JP JP19719787A patent/JPS6439999A/en active Pending
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| ES8600783A1 (en) | Biosynthetically produced human nerve growth factor, process for producing it, compositions containing it, DNA sequence encoding it, vectors containing the sequence and host cells transformed thereby. | |
| IE820069L (en) | Recombinant dna encoding a polypeptide | |
| GEP19970775B (en) | Method for the production of human erythropoietin | |
| DK204487A (en) | PREPARATION OF PLURIPOTENT GRANULOCYT COLONY STIMULATING FACTOR | |
| BG60250B1 (en) | METHOD FOR OBTAINING TUMOR NECROSIS FACTOR | |
| DE3586189D1 (en) | QUANTITY AND METHOD FOR PRODUCING RECOMBINED PRODUCTS IN LARGE YIELD. | |
| AR241654A1 (en) | Human tissue plasminogen activator, pharmaceutical compositions containing it, processes for making it, and dna and transformed cell intermediates therefor | |
| ATE88501T1 (en) | RECOMBINANT DNA EXPRESSION VECTORS. | |
| GB2160206B (en) | Dna sequence coding for an enzyme cleavage site | |
| ES8307297A1 (en) | Polypeptides, process for their microbial production, intermediates therefor and compositions containing them. | |
| HUP9900878A2 (en) | High methionine derivatives of alpa-hordothionin | |
| FI90564C (en) | A method of preparing a bovine, porcine or human growth hormone polypeptide analog | |
| BG38166A3 (en) | METHOD FOR OBTAINING A POLYPEPTIDE INCLUDING EXPRESSION OF ITS HETEROLOGOUS GENE FROM A RECOMBINANT MICROBIAL CLONING VECTOR | |
| PT77167A (en) | Process for preparing yeast hybrid vectors and their use for the production of polypeptides | |
| AU6801487A (en) | Method of producing a recombinant protein complex having human factor VIII:C activity | |
| EP0539590A4 (en) | ||
| IL88151A0 (en) | Interleukin-7,its preparation and compositions containing it | |
| EP0275204A3 (en) | Production of fibroblast growth factor | |
| SE8701934L (en) | UNCLAIMED EXPRESSION OF HUMAN INTERLEUKIN-2 IN CANCER CELLS | |
| DE69012377D1 (en) | Polypeptide and its production. | |
| ES2076152T3 (en) | DEFINITION AND STRUCTURE OF GENES FOR PROTEASE A AND PROTEASE B FROM STREPTOMICES GRISEUS. | |
| JPS642572A (en) | Polypeptide, dna and use thereof | |
| DK0387646T3 (en) | Achromobacter protease I gene and gene product thereof | |
| JPS6439999A (en) | Stabilization of myc protein, variant myc gene, recombinant plasmid containing said gene and production of variant myc protein using transformant by said recombinant plasmid | |
| DK587488D0 (en) | FIBROBLAST GROWTH FACTOR, NUCLEOTIDE SEQUENCE CODING THEREFORE, PREPARATION CONTAINING THE FACTOR AND PROCEDURES FOR PRODUCING THEREOF |