JPS6443502A - Fractionation of hyaluronic acid - Google Patents
Fractionation of hyaluronic acidInfo
- Publication number
- JPS6443502A JPS6443502A JP19908787A JP19908787A JPS6443502A JP S6443502 A JPS6443502 A JP S6443502A JP 19908787 A JP19908787 A JP 19908787A JP 19908787 A JP19908787 A JP 19908787A JP S6443502 A JPS6443502 A JP S6443502A
- Authority
- JP
- Japan
- Prior art keywords
- salt
- cpd
- hyaluronic acid
- org
- fractionated
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- KIUKXJAPPMFGSW-DNGZLQJQSA-N (2S,3S,4S,5R,6R)-6-[(2S,3R,4R,5S,6R)-3-Acetamido-2-[(2S,3S,4R,5R,6R)-6-[(2R,3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-DNGZLQJQSA-N 0.000 title abstract 5
- 229920002674 hyaluronan Polymers 0.000 title abstract 5
- 229960003160 hyaluronic acid Drugs 0.000 title abstract 5
- 238000005194 fractionation Methods 0.000 title abstract 3
- 150000003839 salts Chemical class 0.000 abstract 6
- 125000002091 cationic group Chemical group 0.000 abstract 3
- 239000002904 solvent Substances 0.000 abstract 3
- 239000004094 surface-active agent Substances 0.000 abstract 3
- 239000002253 acid Substances 0.000 abstract 2
- -1 cetyltrimethylammonium halide Chemical class 0.000 abstract 2
- 238000004587 chromatography analysis Methods 0.000 abstract 2
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 abstract 1
- 229960004830 cetylpyridinium Drugs 0.000 abstract 1
- 239000002131 composite material Substances 0.000 abstract 1
- 238000010494 dissociation reaction Methods 0.000 abstract 1
- 230000005593 dissociations Effects 0.000 abstract 1
- 238000002523 gelfiltration Methods 0.000 abstract 1
- 239000002808 molecular sieve Substances 0.000 abstract 1
- 235000017281 sodium acetate Nutrition 0.000 abstract 1
- 239000001632 sodium acetate Substances 0.000 abstract 1
- URGAHOPLAPQHLN-UHFFFAOYSA-N sodium aluminosilicate Chemical compound [Na+].[Al+3].[O-][Si]([O-])=O.[O-][Si]([O-])=O URGAHOPLAPQHLN-UHFFFAOYSA-N 0.000 abstract 1
Landscapes
- Polysaccharides And Polysaccharide Derivatives (AREA)
Abstract
PURPOSE:To fractionate hyaluronic acid or a salt thereof in a small width of MW distribution and a desired size, by processing said acid or said salt into a conjugated cpd. with a cationic surface active agent and applying the cpd. to a molecular sieve. CONSTITUTION:Hyaluronic acid or a salt thereof is processed into a conjugated cpd. with a cationic surface active agent. The cpd., dissolved in a water-soluble org. solvent, is fractionated by means of chromatography, and is subsequently dissociated to give a fractionated hyaluronic acid or salt thereof. The cpd. can be obtd. by adding dropwise a cationic surface active agent, e.g., cetyltrimethylammonium halide or cetylpyridinium halide, to an aq. soln. of hyaluronic acid or a salt thereof and mixing them. The chromatography is carried out using, for example, a gel filtration or gel permeation carrier having a desired fractionation MW range and having been washed with an org. solvent of the same kind as the one dissolving the composite. The dissociation of the conjugate is carried out by adding to the fractionated soln. an inorg. salt of an org. acid soluble in the org. solvent used for the fractionation (e.g., sodium acetate).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP62199087A JPH0813847B2 (en) | 1987-08-11 | 1987-08-11 | Fractionation method of hyaluronic acid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP62199087A JPH0813847B2 (en) | 1987-08-11 | 1987-08-11 | Fractionation method of hyaluronic acid |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS6443502A true JPS6443502A (en) | 1989-02-15 |
| JPH0813847B2 JPH0813847B2 (en) | 1996-02-14 |
Family
ID=16401886
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP62199087A Expired - Lifetime JPH0813847B2 (en) | 1987-08-11 | 1987-08-11 | Fractionation method of hyaluronic acid |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH0813847B2 (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH05508183A (en) * | 1991-04-19 | 1993-11-18 | フィディーア・ソシエタ・ペル・アチオニ | Hyaluronic acid purification method and fractionation of pure hyaluronic acid for ophthalmology |
| US6946551B2 (en) | 2003-03-12 | 2005-09-20 | New Life Resources, Llc | Preparation of hyaluronic acid from eggshell membrane |
| US8580315B2 (en) | 2004-03-10 | 2013-11-12 | Esm Technologies, Llc | Anti-inflammatory activity of eggshell membrane and processed eggshell membrane preparations |
| JP2014101470A (en) * | 2012-11-22 | 2014-06-05 | Sumitomo Bakelite Co Ltd | Sugar chain purification method |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS609042A (en) * | 1983-06-09 | 1985-01-18 | ジ−・テイ−・イ−・プロダクツ・コ−ポレイシヨン | Single-ended metal halogen lamp using ionized potential selection of added gas and method of producing same |
| JPS60133894A (en) * | 1983-11-25 | 1985-07-17 | マイルス・ラボラトリース・インコーポレーテツド | Hyaluronic acid and its manufacturing method |
-
1987
- 1987-08-11 JP JP62199087A patent/JPH0813847B2/en not_active Expired - Lifetime
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS609042A (en) * | 1983-06-09 | 1985-01-18 | ジ−・テイ−・イ−・プロダクツ・コ−ポレイシヨン | Single-ended metal halogen lamp using ionized potential selection of added gas and method of producing same |
| JPS60133894A (en) * | 1983-11-25 | 1985-07-17 | マイルス・ラボラトリース・インコーポレーテツド | Hyaluronic acid and its manufacturing method |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH05508183A (en) * | 1991-04-19 | 1993-11-18 | フィディーア・ソシエタ・ペル・アチオニ | Hyaluronic acid purification method and fractionation of pure hyaluronic acid for ophthalmology |
| US6946551B2 (en) | 2003-03-12 | 2005-09-20 | New Life Resources, Llc | Preparation of hyaluronic acid from eggshell membrane |
| US8580315B2 (en) | 2004-03-10 | 2013-11-12 | Esm Technologies, Llc | Anti-inflammatory activity of eggshell membrane and processed eggshell membrane preparations |
| JP2014101470A (en) * | 2012-11-22 | 2014-06-05 | Sumitomo Bakelite Co Ltd | Sugar chain purification method |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0813847B2 (en) | 1996-02-14 |
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