JPS6467184A - Method for cultivating by aeration in liquid and apparatus therefor - Google Patents

Method for cultivating by aeration in liquid and apparatus therefor

Info

Publication number
JPS6467184A
JPS6467184A JP62221914A JP22191487A JPS6467184A JP S6467184 A JPS6467184 A JP S6467184A JP 62221914 A JP62221914 A JP 62221914A JP 22191487 A JP22191487 A JP 22191487A JP S6467184 A JPS6467184 A JP S6467184A
Authority
JP
Japan
Prior art keywords
liquid
water repellent
repellent material
aeration
constituted
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP62221914A
Other languages
Japanese (ja)
Other versions
JPH0560353B2 (en
Inventor
Masahiko Ishida
Yusaku Nishimura
Tetsuo Yamaguchi
Kazuyuki Ito
Harumi Matsuzaki
Kenji Baba
Setsuo Saito
Toshio Yahagi
Masayoshi Kubota
Ryoichi Haga
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Hitachi Ltd
Original Assignee
Hitachi Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hitachi Ltd filed Critical Hitachi Ltd
Priority to JP62221914A priority Critical patent/JPS6467184A/en
Priority to EP88907809A priority patent/EP0336966B1/en
Priority to PCT/JP1988/000890 priority patent/WO1989002458A1/en
Priority to DE3850652T priority patent/DE3850652T2/en
Publication of JPS6467184A publication Critical patent/JPS6467184A/en
Publication of JPH0560353B2 publication Critical patent/JPH0560353B2/ja
Granted legal-status Critical Current

Links

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  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

PURPOSE:To effectively crush bubbles formed by passing a gas through an aqueous culture fluid containing organic cells, by bringing the bubbles into contact with a porous defoaming layer constituted of a water repellent material placed on a liquid surface. CONSTITUTION:An animal cell, plant cell or microbical cell is cultivated in a readily foamable liquid culture medium by aeration. In the process, bubbles formed by passing a gas through a liquid are brought into contact with a porous defoaming layer (preferably >=50% opening ratio and 2-50mm opening diameter) constituted of a water repellent material (preferably silane or siloxane) placed on a liquid surface to carry out cultivation in the liquid by aeration while crushing the bubbles. A defoaming layer is constituted of a water repellent material forming >=30 deg. contact angle between a horizontal surface and droplets of the culture fluid or a material coated or impregnated with the afore- mentioned water repellent material on the surface thereof is used as the above- mentioned defoaming layer. This method is especially effective in using an aqueous culture fluid (e.g. culture medium containing blood serum added thereto) having >=45dyn/cm<2>, especially >=90dyn/cm<2> surface tension.
JP62221914A 1987-09-07 1987-09-07 Method for cultivating by aeration in liquid and apparatus therefor Granted JPS6467184A (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
JP62221914A JPS6467184A (en) 1987-09-07 1987-09-07 Method for cultivating by aeration in liquid and apparatus therefor
EP88907809A EP0336966B1 (en) 1987-09-07 1988-09-05 Cell culture method and apparatus
PCT/JP1988/000890 WO1989002458A1 (en) 1987-09-07 1988-09-05 Cell culture method and apparatus
DE3850652T DE3850652T2 (en) 1987-09-07 1988-09-05 CELL GROWING METHOD AND DEVICE.

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP62221914A JPS6467184A (en) 1987-09-07 1987-09-07 Method for cultivating by aeration in liquid and apparatus therefor

Publications (2)

Publication Number Publication Date
JPS6467184A true JPS6467184A (en) 1989-03-13
JPH0560353B2 JPH0560353B2 (en) 1993-09-02

Family

ID=16774137

Family Applications (1)

Application Number Title Priority Date Filing Date
JP62221914A Granted JPS6467184A (en) 1987-09-07 1987-09-07 Method for cultivating by aeration in liquid and apparatus therefor

Country Status (1)

Country Link
JP (1) JPS6467184A (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10135277A1 (en) * 2001-07-13 2003-02-06 Jostra Ag Device with a surface coated with a defoaming agent and use of a surface with a defoaming agent
JPWO2006106748A1 (en) * 2005-03-30 2008-09-11 国立大学法人名古屋大学 Bio-organism production method and culture vessel

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10135277A1 (en) * 2001-07-13 2003-02-06 Jostra Ag Device with a surface coated with a defoaming agent and use of a surface with a defoaming agent
DE10135277C2 (en) * 2001-07-13 2003-06-05 Jostra Ag Defoaming agent, device with a surface coated with the defoaming agent and use of such a device
US7273466B2 (en) 2001-07-13 2007-09-25 Jostra Ag Apparatus having a defoamer-coated surface and use of a defoamer-containing surface
JPWO2006106748A1 (en) * 2005-03-30 2008-09-11 国立大学法人名古屋大学 Bio-organism production method and culture vessel
US7951584B2 (en) 2005-03-30 2011-05-31 National University Corporation Nagoya University Method for production of biological organic material and culture vessel

Also Published As

Publication number Publication date
JPH0560353B2 (en) 1993-09-02

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