JPS6471497A - Production of bradykinin - Google Patents

Production of bradykinin

Info

Publication number
JPS6471497A
JPS6471497A JP22612787A JP22612787A JPS6471497A JP S6471497 A JPS6471497 A JP S6471497A JP 22612787 A JP22612787 A JP 22612787A JP 22612787 A JP22612787 A JP 22612787A JP S6471497 A JPS6471497 A JP S6471497A
Authority
JP
Japan
Prior art keywords
bradykinin
pblak1
coli
liquid chromatography
performance liquid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP22612787A
Other languages
Japanese (ja)
Other versions
JPH0364118B2 (en
Inventor
Masahiro Iwakura
Shinichi Ohashi
Tsukasa Sakai
Yoshio Tanaka
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
National Institute of Advanced Industrial Science and Technology AIST
Original Assignee
Agency of Industrial Science and Technology
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Agency of Industrial Science and Technology filed Critical Agency of Industrial Science and Technology
Priority to JP22612787A priority Critical patent/JPS6471497A/en
Publication of JPS6471497A publication Critical patent/JPS6471497A/en
Publication of JPH0364118B2 publication Critical patent/JPH0364118B2/ja
Granted legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
    • C07K7/04—Linear peptides containing only normal peptide links
    • C07K7/18—Kallidins; Bradykinins; Related peptides

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

PURPOSE:To obtain bradykinin having hypotensive action, by separating bacterial cell from E.coli C600 strain containing pBLAK1, treating the cell with cyanogen bromide and separating the objective compound by high-performance liquid chromatography. CONSTITUTION:E.coli C600 strain containing recombinant plasmid pBLAK1 is cultured in a medium, the bacterial cells are collected after cultivation and centrifuged to obtain a protein composed of dihydrofolic acid reductase-reductase fused to bradykinin. The protein is mixed and treated with cyanogen bromide and subjected to high-performance liquid chromatography to obtain bradykinin useful in the fields of medical and pharmaceutical industries, etc.
JP22612787A 1987-09-09 1987-09-09 Production of bradykinin Granted JPS6471497A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP22612787A JPS6471497A (en) 1987-09-09 1987-09-09 Production of bradykinin

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP22612787A JPS6471497A (en) 1987-09-09 1987-09-09 Production of bradykinin

Publications (2)

Publication Number Publication Date
JPS6471497A true JPS6471497A (en) 1989-03-16
JPH0364118B2 JPH0364118B2 (en) 1991-10-03

Family

ID=16840264

Family Applications (1)

Application Number Title Priority Date Filing Date
JP22612787A Granted JPS6471497A (en) 1987-09-09 1987-09-09 Production of bradykinin

Country Status (1)

Country Link
JP (1) JPS6471497A (en)

Also Published As

Publication number Publication date
JPH0364118B2 (en) 1991-10-03

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Legal Events

Date Code Title Description
EXPY Cancellation because of completion of term