JPS6474988A - Synthetic gene of cystatin c, corresponding recombinant plasmid and recombinant - Google Patents

Synthetic gene of cystatin c, corresponding recombinant plasmid and recombinant

Info

Publication number
JPS6474988A
JPS6474988A JP22847787A JP22847787A JPS6474988A JP S6474988 A JPS6474988 A JP S6474988A JP 22847787 A JP22847787 A JP 22847787A JP 22847787 A JP22847787 A JP 22847787A JP S6474988 A JPS6474988 A JP S6474988A
Authority
JP
Japan
Prior art keywords
gene
cystatin
recombinant
fragment
amino acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP22847787A
Other languages
Japanese (ja)
Inventor
Yoshimasa Ike
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Mitsui Toatsu Chemicals Inc
Original Assignee
Mitsui Toatsu Chemicals Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mitsui Toatsu Chemicals Inc filed Critical Mitsui Toatsu Chemicals Inc
Priority to JP22847787A priority Critical patent/JPS6474988A/en
Publication of JPS6474988A publication Critical patent/JPS6474988A/en
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/8107—Endopeptidase (E.C. 3.4.21-99) inhibitors
    • C07K14/8139—Cysteine protease (E.C. 3.4.22) inhibitors, e.g. cystatin

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

PURPOSE:To produce protein of cystatin C by using recombinant DNA technology, by chemically or biochemically synthesizing a gene of cystatin C based on a well-known amino acid sequence and using the gene. CONSTITUTION:Based on a previously well-known amino acid sequence, three points of (i) the most acceptable codon is selected in Escherichia coli useful in manifestation of synthetic gene, (ii) a palindrome on any fragment or a duplex is avoided as much as possible and (iii) a stop codon and an attached end DNA for insertion to a vector are set besides DNA sequence to code a gene of cystatin C are taken into consideration, 13 fragments are decomposed, a gene is designed, each fragment is synthesized and linked to give a complete gene of cystatin C. The gene is integrated into a proper a manifestation vector to give a recombinant plasmid, by which a host is transformed and the transformant is cultivated to produce and to recover protein of cystatin C.
JP22847787A 1987-09-14 1987-09-14 Synthetic gene of cystatin c, corresponding recombinant plasmid and recombinant Pending JPS6474988A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP22847787A JPS6474988A (en) 1987-09-14 1987-09-14 Synthetic gene of cystatin c, corresponding recombinant plasmid and recombinant

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP22847787A JPS6474988A (en) 1987-09-14 1987-09-14 Synthetic gene of cystatin c, corresponding recombinant plasmid and recombinant

Publications (1)

Publication Number Publication Date
JPS6474988A true JPS6474988A (en) 1989-03-20

Family

ID=16877086

Family Applications (1)

Application Number Title Priority Date Filing Date
JP22847787A Pending JPS6474988A (en) 1987-09-14 1987-09-14 Synthetic gene of cystatin c, corresponding recombinant plasmid and recombinant

Country Status (1)

Country Link
JP (1) JPS6474988A (en)

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