JPS6479200A - Human placental decidual cell-derived protein - Google Patents
Human placental decidual cell-derived proteinInfo
- Publication number
- JPS6479200A JPS6479200A JP62177801A JP17780187A JPS6479200A JP S6479200 A JPS6479200 A JP S6479200A JP 62177801 A JP62177801 A JP 62177801A JP 17780187 A JP17780187 A JP 17780187A JP S6479200 A JPS6479200 A JP S6479200A
- Authority
- JP
- Japan
- Prior art keywords
- cell
- human placental
- 000mol
- derived protein
- measured
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 230000003169 placental effect Effects 0.000 title abstract 3
- 102000004169 proteins and genes Human genes 0.000 title abstract 3
- 108090000623 proteins and genes Proteins 0.000 title abstract 3
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 abstract 2
- 238000002523 gelfiltration Methods 0.000 abstract 2
- 241000283690 Bos taurus Species 0.000 abstract 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 abstract 1
- 125000000539 amino acid group Chemical group 0.000 abstract 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 abstract 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 abstract 1
- 235000011130 ammonium sulphate Nutrition 0.000 abstract 1
- 239000002246 antineoplastic agent Substances 0.000 abstract 1
- 239000007853 buffer solution Substances 0.000 abstract 1
- 230000010261 cell growth Effects 0.000 abstract 1
- 238000004587 chromatography analysis Methods 0.000 abstract 1
- 239000012141 concentrate Substances 0.000 abstract 1
- 238000001962 electrophoresis Methods 0.000 abstract 1
- 230000001605 fetal effect Effects 0.000 abstract 1
- 239000001963 growth medium Substances 0.000 abstract 1
- 230000002209 hydrophobic effect Effects 0.000 abstract 1
- 229940125721 immunosuppressive agent Drugs 0.000 abstract 1
- 239000003018 immunosuppressive agent Substances 0.000 abstract 1
- 230000002401 inhibitory effect Effects 0.000 abstract 1
- 238000004255 ion exchange chromatography Methods 0.000 abstract 1
- 238000004811 liquid chromatography Methods 0.000 abstract 1
- 239000000463 material Substances 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 238000002360 preparation method Methods 0.000 abstract 1
- 210000002966 serum Anatomy 0.000 abstract 1
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 abstract 1
- 239000006228 supernatant Substances 0.000 abstract 1
Landscapes
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Abstract
NEW MATERIAL:A protein having a sequence of 20 amino acid residues at the N-terminal expressed by the formula, about 49,000mol.wt. measured by an electrophoresis (SDS-PAGE), about 63,000mol.wt. measured by a gel filtration method (TSK Gel G 3000SWXL(R)) and cell growth inhibitory activity. USE:An immunosuppressive agent and anticancer agent. PREPARATION:For example, TTK-1 cell strain which is a human placental decidual cell is cultivated using a 10% bovine fetal serum-containing culture medium and centrifuged to collect a cultivation supernatant, which is then ultrafiltered and concentrated. The resultant concentrate is subsequently salted out with ammonium sulfate to provide a deposited fraction, which is then dissolved in a 0.01M phosphoric acid buffer solution and purified by gel filtration, ion exchange chromatography, high-speed liquid chromatography and hydrophobic chromatography, etc., to afford the aimed human placental decidual cell- derived protein.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP62177801A JPH07108914B2 (en) | 1987-07-15 | 1987-07-15 | Cytostatic protein |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP62177801A JPH07108914B2 (en) | 1987-07-15 | 1987-07-15 | Cytostatic protein |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS6479200A true JPS6479200A (en) | 1989-03-24 |
| JPH07108914B2 JPH07108914B2 (en) | 1995-11-22 |
Family
ID=16037327
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP62177801A Expired - Lifetime JPH07108914B2 (en) | 1987-07-15 | 1987-07-15 | Cytostatic protein |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH07108914B2 (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5516754A (en) * | 1989-03-10 | 1996-05-14 | Loefflmann; Adolf | Method for the selective dissolution of cancer cells using a composition derived from the photosynthetic system of plants and mammalian embryonic tissue |
-
1987
- 1987-07-15 JP JP62177801A patent/JPH07108914B2/en not_active Expired - Lifetime
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5516754A (en) * | 1989-03-10 | 1996-05-14 | Loefflmann; Adolf | Method for the selective dissolution of cancer cells using a composition derived from the photosynthetic system of plants and mammalian embryonic tissue |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH07108914B2 (en) | 1995-11-22 |
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