KR101242579B1 - Preparing method of Gel paste for treatment an ulcer including a honey by radiation and Gel paste for treatment an ulcer thereby - Google Patents

Abstract

The present invention relates to a method for preparing an ulcer paste for treating ulcers comprising honey using radiation, and to preparing an aqueous solution by dissolving a biocompatible polymer in purified water in detail (step 1); Pouring the aqueous solution into a tray to form a gel sheet (step 2); Irradiating radiation to the aqueous solution formed of the gel sheet of step 2 (step 3); Lyophilizing the irradiated gel of step 3 (step 4); Grinding the lyophilized gel of step 4 (step 5); And dissolving the gel, honey and the preservative pulverized in step 5 in purified water to prepare a paste (step 6).
The paste prepared by the above manufacturing method has basic characteristics for use for wound treatment, water evaporation reduction, prevention of sensitization from body fluid absorbing bacteria, adhesion to wounds or skin, transparency, ease of handling, storage and sterilization properties. It has an effect that can be applied as an antibiotic.

Description

Preparation method of gel paste for treatment an ulcer including a honey by radiation and Gel paste for treatment an ulcer Thus

The present invention relates to a method for preparing an ulcer paste containing honey using a radiation, and a paste for treating an ulcer prepared thereby.

An ulcer means that the epithelium has fallen out due to inflammation or necrosis, and the surface of the tissue is locally missing or recessed.If the inflammation of the tissue progresses, the tissue is destroyed by lysosomal enzymes from neutrophils and macrophages. Ulcers can occur when there is a problem with the arteries or veins that supply oxygen and nutrients, causing a sudden or slow blood supply. That is, all the causes that can cause inflammation is the cause of the ulcers, and inflammation by the bacterial infection proceeds and the formation of abscesses, part of the normal tissue is destroyed, and further develops ulcers.

Due to this development, ulcers can occur in the skin, as well as in the digestive, respiratory, cardiovascular, and genitourinary organs. In particular, the ulcers due to the lack of oxygen and nutrients in the tissue are typical of diabetic ulcers that occur mainly on the feet or legs, a complication due to diabetes.

Honey has been used as a folk remedy for trauma and stomatitis in the past, but honey has recently been reexamined as an antibiotic. In particular, as the concern about antibiotic resistance increases, research on the bactericidal ability of honey is being conducted.

Honey's ability to control bacteria has been transmitted since BC, and it has been called INHIBINE as an inhibitor of the unknown bacteria that honey has. In recent years, the scientific effects of honey on surgical wounds have been reported by Bromfield (1973) and Keast-Butler (1980) to be effective as a treatment for ulcers, particularly as a treatment for intractable ulcers. (Effem, 1988: McInerly, 1990; Zulma, 1989).

Willix et al. (1992) demonstrated the ability of honey to inhibit seven types of bacteria that are primarily involved in trauma. El-sukhon et al. (1994) found that honey's ability to inhibit bacteria was more effective in negative than in Gram-positive. It was reported that Bacillus cereus inhibits spore germination.

The bacteriostatic ability is related to the osmotic pressure caused by the hypertonic solution according to the high sugar content of honey and the low pH of the honey, but it is estimated to be mainly due to the hydrogen peroxide content (Bogodanov, 1984,1997; Allen et al., 1991; Mollan, 1992)

Therefore, the present inventors use the bactericidal ability of honey, which has been used as a folk remedy, to reduce the amount of evaporation of water than conventional therapeutics, to create an optimal wet environment for a long time, and to improve the adhesion and flexibility by the addition of honey. The ulcer containing paste was completed.

It is an object of the present invention to provide a method for preparing an ulcer paste for treating ulcers comprising honey and a ulcer paste prepared for ulceration.

In order to achieve the above object, the present invention comprises the steps of preparing an aqueous solution by dissolving the biocompatible polymer in purified water (step 1); Pouring the aqueous solution into a tray to form a gel sheet (step 2); Irradiating radiation to the aqueous solution formed of the gel sheet of step 2 (step 3); Lyophilizing the irradiated gel of step 3 (step 4); Grinding the lyophilized gel of step 4 (step 5); And dissolving the gel, honey and the preservative pulverized in step 5 in purified water to prepare a paste (step 6).

The paste prepared by the method for preparing an ulcer paste containing honey using the radiation of the present invention is a basic property for use in wound healing, reduced water evaporation amount, prevention of sensitization from body fluid absorption bacteria, adhesion to wounds or skin It has the properties of transparency, transparency, ease of handling, storage and sterilization, and can be applied as an antibiotic.

1 is a schematic manufacturing process diagram of the manufacturing method of the present invention;
2 is a graph of water vaporization according to Experimental Example 1 of the present invention;
Figure 3 is a graph of the results of ulcer treatment animals according to Experimental Example 2 of the present invention.

EMBODIMENT OF THE INVENTION Hereinafter, this invention is demonstrated in detail.

The present invention comprises the steps of dissolving the biocompatible polymer in purified water to prepare an aqueous solution (step 1); Pouring the aqueous solution into a tray to form a gel sheet (step 2); Irradiating radiation to the aqueous solution formed of the gel sheet of step 2 (step 3); Lyophilizing the irradiated gel of step 3 (step 4); Grinding the lyophilized gel of step 4 (step 5); And dissolving the gel, honey and the preservative pulverized in step 5 in purified water to prepare a paste (step 6).

Hereinafter, the present invention will be described in detail step by step.

Step 1 of the preparation method is a step of dissolving the biocompatible polymer in purified water to prepare an aqueous solution.

At this time, the biocompatible polymer used in step 1 is a synthetic polymer selected from the group consisting of polyvinyl alcohol, polyvinylpyrrolidone, polyacrylic acid and polyethylene oxide or carrageenan, sodium carboxymethyl cellulose, gelatin, agar, alginate and chitosan It is preferable that it is a natural polymer selected from the group which consists of, and it is more preferable that it is sodium carboxymethyl cellulose. The sodium carboxymethyl cellulose has the advantage that it can be used in the paste of the present invention because it has the property to contain water by including a hydrophilic functional group as a three-dimensional network structure.

In addition, the weight ratio of the biocompatible polymer to the purified water in step 1 is preferably 1 to 30% by weight.

If the biocompatible polymers to be mixed are less than 1% by weight, the viscosity is very low, making it difficult to prepare a paste. If the biocompatible polymer is more than 30% by weight, it is difficult to dissolve the biocompatible polymers and apply the wound to the wound. It is difficult and there is a problem that can cause secondary wounds on ulcers after drying.

Step 2 of the manufacturing method is a step of forming the gel sheet by pouring the aqueous solution prepared in step 1 to the tray.

In the step 2, the aqueous solution obtained in the step 1 is poured into a tray and left to be molded at room temperature in the form of a gel sheet. When the temperature of the aqueous solution is lowered to 40 ° C. or lower, the gel is physically gelled due to the characteristics of the biocompatible polymer and the sheet is removed from the tray. Is formed. The tray in the present invention can be used in a general shape or a variety of sizes, thicknesses and shapes depending on the application, it is not limited thereto.

Step 3 of the manufacturing method is a step of irradiating the gel sheet formed in step 2, through which the polymer is crosslinked and sterilized at the same time.

By crosslinking the polymer in step 3 can be obtained a hydrogel of the desired physical properties, wherein the radiation used may be gamma rays, ultraviolet rays and electron beams.

In this case, the radiation dose is preferably 2 to 200 kGy. If less than 2 kGy can not be expected to form an effective cross-linking between the biocompatible polymers by irradiation, if it exceeds 200 kGy, the flexibility of the hydrogel is reduced due to the increase in the gel strength due to the increase in the amount of crosslinking, Radiation deterioration problem occurs.

Step 4 of the preparation method is a step of freeze drying the gel irradiated in step 3.

Since freeze-drying is not cause a transition to the liquid phase in the process of freeze-ray of an object, and the sublimation drying the frozen object to the drying method of, rather than dried to vaporize the liquid phase, the solid phase that is sublimated to H ₂ O in the frozen state object The various components contained in it are preserved without volatilization. Also, during the freezing process, the water is crystallized to form agglomerates and freezes, so it can be seen that a small channel is lost on the surface after drying. It has the advantage that it can be used when needed after storage.

Because of this, the gel lyophilized through step 4 of the present invention has the advantage that water vaporization is suppressed.

Step 5 of the preparation method is a step of grinding the lyophilized gel in step 4. Step 5 allows the formation of a gel of small particle size suitable for use in paste preparation.

The grinding of step 5 is preferably selected from the group comprising a homogenizer, an extruder and a mixer, without being limited thereto.

Step 6 of the preparation method is a step of preparing a paste by dissolving the gel, honey and preservatives pulverized in step 5 in purified water.

At this time, the weight ratio in which the ground gel is mixed as a paste is preferably 1 to 5% by weight. If less than 1% by weight, it is difficult to maintain the strength enough to accommodate the drug to treat the wound, if the amount exceeds 5% by weight is difficult to produce a paste.

The weight ratio of the honey is mixed as a paste in step 6 is preferably 15 to 25% by weight.

If less than 15% by weight, there is a problem that can not sufficiently improve the adhesive strength and flexibility, if more than 25% by weight will cause a problem that can not maintain the strength enough to accommodate the drug.

On the other hand, the preservative added in step 6 is used to prevent the deterioration of the paste, it is effective as an antioxidant to prevent the aging of the skin while acting as a antiseptic, antiseptic. In this case, it is preferable to use methyl paraben, ethyl paraben, propyl paraben, butyl paraben, isobutyl paraben, and phenoxy ethanol, and the weight ratio of the preservative to be mixed as a paste is preferably 0.01 to 0.1% by weight.

If the amount of the preservative added is 0.01% by weight or less, it is difficult to prevent deterioration of the paste as sterilization and preservative, and when it is contained in 0.1% by weight or more, it is difficult to expect the improvement of the effect due to the content increase.

In addition, the present invention provides a ulcer treatment paste prepared by the above production method.

The ulcer paste according to the present invention has basic properties for use in wound healing, reduced water evaporation amount, prevention of infection from body fluid-absorbing bacteria, adhesion to wounds or skin, transparency, ease of handling, storage and sterilization properties. This has the effect of being applied as an antibiotic.

Hereinafter, the present invention will be described in more detail by the following examples.

However, the following examples merely illustrate the present invention, and the contents of the present invention are not limited by the following examples.

≪ Example 1 >

Preparation of ulcer pastes for treatment 1

Step 1: An aqueous solution was prepared by dissolving 20 g of sodium carboxyl methyl cellulose in 80 g of purified water as a biocompatible polymer.

Step 2: The aqueous solution prepared in Step 1 was poured into a tray and left at room temperature to form a hydrogel in a sheet form.

Step 3: The gel formed in Step 2 was irradiated with gamma rays to a total dose of 75 kGy to crosslink and sterilize the polymer.

Step 4: The irradiated gel in step 3 was lyophilized.

Step 5: The lyophilized gel in step 4 was ground through a homogenizer.

Step 6: A paste was prepared by dissolving 5 g of the gel ground in step 5, 20 g of Manuka honey, and 0.02 g of propyl paraben in 74.98 g of purified water.

<Example 2>

Preparation of ulcer pastes for treatment 2

Example 1 was carried out in the same manner as in Example 1 except that chestnut honey instead of Manuka honey in step 6 of.

<Example 3>

Preparation of ulcer pastes 3

Except for using rapeseed honey instead of Manuka honey in step 6 of Example 1 was carried out in the same manner as in Example 1.

<Example 4>

Preparation of ulcer pastes 4

Except for using Acacia honey instead of Manuka honey in step 6 of Example 1 was carried out in the same manner as in Example 1.

<Example 5>

Preparation of ulcer pastes 5

Except for using honey honey instead of Manuka honey in step 6 of Example 1 was carried out in the same manner as in Example 1.

&Lt; Comparative Example 1 &

Except for using propylene glycol instead of Manuka honey in step 6 of Example 1 was carried out in the same manner as in Example 1.

Comparative Example 2

Commercial Intrasite gel from Smith & Nephew was used.

<Experimental Example 1>

Moisture Evaporation Analysis of Pastes

In the present invention, the paste for treating ulcers prepared according to Examples 1 to 5 and the materials of Comparative Examples 1 and 2 were placed in a Petri dish, and placed in a constant temperature water bath controlled at 37 ° C. to measure the degree of moisture evaporation in the human body. The change of evaporation rate was measured and the result is shown in FIG.

As shown in FIG. 2, the paste prepared according to the embodiments of the present invention showed that the evaporation of water proceeded more slowly than the paste using propylene glycol and commercial products. .

Through this, it can be seen that the paste provided by the present invention has a superior effect than conventional materials in view of the purpose of treating ulcers.

<Experimental Example 2>

Ulcer Treatment Animal Test Results

After anesthetizing the DB / DB mouse, the hair of the trochanter was cut and recuperated, and the ulcer was applied for 24 hours by applying a pressure of 140 mmHg for 24 hours using a 1 cm diameter stainless steel pressure rod as a pressure device. Caused. After washing the ulcer area with physiological saline, the ulcer treatment effect of the ulcer treatment paste prepared according to Examples 1 to 5 and the compounds of Comparative Examples 1 and 2 and mice undressed was observed. The results are shown in FIG. 3.

Ulcer healing rate shown in Figure 3 was calculated by the following equation (1).

&Quot; (1) &quot;

As shown in FIG. 3, when the dressing treatment is not performed after the ulcer treatment, the ulcer area may be gradually treated after the ulcer area is increased. However, in the case of the paste prepared according to Examples 1 to 5 of the present invention, it can be seen that the ulcer area decreases rapidly. In addition, when compared with Comparative Examples 1 and 2 it was found that the ulcer treatment effect is faster, it was confirmed that the time until the ulcer is cured nearly 10 days.

Through this, it was confirmed that the paste for treating ulcers containing honey of the present invention has an excellent ulcer treatment effect than a paste and a commercial product using propylene glycol.

Claims (10)

Dissolving the biocompatible polymer in purified water to prepare an aqueous solution (step 1); Pouring the aqueous solution into a tray to form a gel sheet (step 2);
Irradiating radiation to the aqueous solution formed of the gel sheet of step 2 (step 3);
Lyophilizing the irradiated gel of step 3 (step 4);
Grinding the lyophilized gel of step 4 (step 5);
And preparing a paste by dissolving the gel, honey, and preservative pulverized in step 5 in purified water (step 6), wherein the biocompatible polymer is polyvinyl alcohol, polyvinylpyrrolidone, polyacrylic acid, and polyethylene oxide. A synthetic polymer selected from the group consisting of or a natural polymer selected from the group consisting of carrageenan, sodium carboxymethyl cellulose, gelatin, agar, alginate and chitosan.
delete The method of claim 1, wherein the weight ratio of the biocompatible polymer mixed in the purified water in step 1 is 1 to 30% by weight.
The method of claim 1, wherein the radiation of step 3 is selected from the group consisting of gamma rays, ultraviolet rays and electron beams.
The method of claim 1, wherein the radiation dose of step 3 is 2 to 200 kG, the method of producing a paste for treating ulcers.
The method of claim 1, wherein the weight ratio of the ground gel of step 6 mixed as a paste is 1 to 10% by weight.
The method of claim 1, wherein the weight ratio of the honey of step 6 is mixed as a paste is 5 to 30% by weight.
The method of claim 1, wherein the preservative of step 6 is selected from the group consisting of methyl paraben, ethyl paraben, propyl paraben, butyl paraben, isobutyl paraben and phenoxy ethanol.
The method of claim 1, wherein the weight ratio of the preservative of step 6 to be mixed into the paste is 0.01 to 0.1% by weight.
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