KR101699127B1 - Composition for accelerating collagen synthesis and its use - Google Patents

Abstract

The present invention relates to fermented products of grape fruit, stem and flower hull; Aging of the fermentation product; Or an extract of the above fermented product or aged product as an active ingredient, and a process for producing the composition.
The present invention also relates to a cosmetic composition, a food composition, a pharmaceutical composition and a quasi-drug composition for skin elasticity or wrinkle improvement or skin regeneration comprising the composition as an active ingredient.
The composition for accelerating collagen synthesis comprising the fermented and aged grape extract according to the present invention as an active ingredient effectively promotes collagen synthesis without substantially affecting the stability of the formulation and has little skin irritation to improve skin elasticity, The effect is excellent.

Description

Composition for Promoting Collagen Synthesis and Use Thereof < RTI ID = 0.0 >

The present invention relates to fermented products of grape fruit, stem and flower hull; Aging of the fermentation product; Or an extract of the above fermented product or aged product as an active ingredient, and a process for producing the composition.

The present invention also relates to a cosmetic composition, a food composition, a pharmaceutical composition and a quasi-drug composition for skin elasticity or wrinkle improvement or skin regeneration comprising the composition as an active ingredient.

Collagen, a major component of the extracellular matrix, is a major substrate protein produced by the fibroblasts of the skin. In addition, it is an important protein occupying about 30% of the total weight of the bioprotein, and has a solid triple helix structure. Collagen forms most of the organic matter in the skin, tendons, bones and teeth, especially in bone and skin (jaw). In most other body structures, it exists as a fibrous inclusion.

Collagen is a relatively weak immunogen, partly due to the blocking of the potential antigenic determinant by the helical structure of the collagen, which also causes the collagen to be resistant to proteolysis. The major functions of collagen are known to be mechanical rigidity of skin, resistance of connective tissues and binding force of tissues, support of cell adhesion, induction of cell division and differentiation (when organism grows or heals the wound) (Van der Rest et al. Ann NY

Acad Sci, 1990).

Such collagen is reduced by aging and photoaging induced by ultraviolet irradiation, which is known to be closely related to skin aging (Arthur K. Balin et al., Aging and the skin, 1989). Collagen also plays an important role in wound healing and can accelerate the synthesis of collagen in the damaged epithelium, allowing quick, scarless wound healing.

Conventionally, products using collagen in a composition for external application for skin such as cosmetics or ointment have been marketed in order to utilize the skin moisturizing effect and wound healing effect of collagen. However, these products apply collagen itself to the skin surface, It is impossible to expect a moisturizing action or a wound healing effect, so that it can not be said that it exhibits essential skin function improvement and wound healing function.

To solve this problem, there has been a growing interest in collagen synthesis promoting substances. Vitamin C, retinoic acid, transforming growth factor (TGF) (Cardinale G. et al. (Japanese Laid-Open Patent Publication No. 1996-231370), betulinic acid (Japanese Laid-Open Patent Publication No. 1996-208424 ), Chlorella extract (JP-A-1997-040523, JP-A-1998-036283), and the like.

However, these materials have limited use due to safety problems such as irritation and reddening when applied to the skin, or the effect is insignificant and virtually no effect of skin function improvement or wound healing can be expected. Therefore, it is urgently required to develop a new collagen synthesis promoting substance which is safer and more effective to the living body than the existing collagen synthesis promoting substance.

In general, antioxidant trends in cosmetics through elasticity and regeneration are becoming common. To this end, the effect was imparted through the enhancement of moisturizing ingredients composed of polyhydric alcohols and oils and the inclusion of the ingredients of effective ingredients capable of providing anti-aging effects. However, in the formulation of cosmetics, stability problems, discoloration / The concentration of the actual effect can not be used or can not be blended because of the reason of. Especially, in case of efficacy raw materials, when cosmetic stability and skin safety are good, practical effects are often insignificant and development of raw materials satisfying all the requirements has been a big issue.

On the other hand, the usual fermented grape extract or fermented and aged grape extract is an extract obtained by fermenting fruit juice after fermenting or fermenting grape fruit, and it is used in cosmetics. However, when the efficacy is good, it precipitates and skin irritation There are many restrictions on the use of the proper concentration.

In order to achieve the above technical object, the present inventors have studied various fermented and aged grape extracts, and have found that grape extract, which is aged for 2 years or more after fermentation of grape fruit, stem, The present inventors have found that the composition exhibits excellent efficacy as compared with the grape extract obtained by fermenting and aging only the fruit, and has excellent stability and skin safety in combination with cosmetics, and has completed the composition for promoting collagen synthesis utilizing the same.

Japanese Laid-Open Patent Publication No. 1996-231370 Japanese Laid-Open Patent Publication No. 1996-208424 Japanese Patent Application Laid-Open No. 1997-040523 Japanese Laid-Open Patent Publication No. 1998-036283

One object of the present invention is to provide a fermentation product of grape fruit, stem and hibiscus; Aging of the fermentation product; Or an extract of the fermented product or the aged product as an active ingredient.

Another object of the present invention is to provide a method for producing grape fruit, comprising the steps of: (a) fermenting grape fruit, stem and hibiscus;

(b) aging the fermentation product obtained in step (a); And

(c) extracting the aged material obtained in the step (b). The present invention also provides a method for producing collagen synthesis promoting composition.

It is still another object of the present invention to provide a cosmetic composition, a food composition, a pharmaceutical composition and a quasi-drug composition for skin elasticity or wrinkle improvement or skin regeneration comprising the composition as an active ingredient.

In order to solve the above problems, in one aspect, the present invention relates to a fermented product of grape fruit, stem and flower hull; Aging of the fermentation product; Or an extract of the fermented product or aged product as an active ingredient.

 The term " flower buds "of the present invention means flower buds, and is a bag (short branch) part connected to a flower bud when one flower blooms on the flower bud. It is also referred to as a vase or flower stalk, and in the present invention, a flower stalk refers to a vine of a vine.

The term "fermentation" of the present invention means a process of decomposing an organic substance using an enzyme possessed by a microorganism. In the present invention, grape fruit, stem and flower hull can be fermented into a microorganism which can be used for ordinary fermentation. The fermentation can be preferably fermentation by yeast, more preferably selected from the group consisting of Saccharomyces , Torulaspora , Zygosaccharomyces or Lactobacillus , But may be fermentation by the genus Saccharomyces , and still more preferably fermentation by Saccharomyces sp., But is not limited thereto.

The term "fermented product" of the present invention includes not only the useful substance itself produced through the fermentation process but also a fermentation strain culture medium in which a fermentation strain and a fermentation product coexist, a fermentation product obtained by centrifuging the fermentation strain from the culture medium, A fermentation product obtained by filtering a fermentation strain from a culture medium, a fermentation product sterilized and filtrated from the culture medium, a dilution solution obtained by diluting the fermentation product, and a product obtained by drying the fermentation product, And may include all kinds of materials including water.

In the present invention, the fermented product is preferably obtained by inoculating yeast with grape fruit, stem, and flower hull.

The term "aging" of the present invention means that a food or the like is allowed to stand for a certain period of time to cause a change or the like due to the action of microorganisms or enzymes or interactions between components. In the present invention, fermented products of grape fruit, As the fermentation proceeds through the fermentation process, its components may change.

The term "aged product" of the present invention refers to a substance in which various components have been changed through aging of the fermented product. The method of aging is not particularly limited and can be aged according to a method commonly used in the art . It is preferably fermented by inoculating yeast into grape fruit, stem, and calyx, and then matured for 2 years in an oak barrel.

The term "extract" of the present invention includes the extract obtained by extracting the fermented product or the aged product, the diluted or concentrated liquid of the extracted liquid, the dried product obtained by drying the extracted liquid, the controlled preparation or purified product of the extracted liquid, Etc., the extract itself and extracts of all the formulations which can be formed using the extract. The extract of the present invention can be preferably used after extraction and filtration.

Preferably, the extract of the present invention can be extracted from fermented products of grape fruit, stem and hibiscus or aged products of the fermented product.

In addition, the method of extracting the fermented product or the aged product in the present invention is not particularly limited, and may be extracted according to a method commonly used in the art. Non-limiting examples of the extraction method include hydrothermal extraction, ultrasonic extraction, filtration or reflux extraction, and they may be performed alone or in combination of two or more methods.

The kind of the extraction solvent used for extracting the fermentation product or the aged product in the present invention is not particularly limited and any solvent known in the art can be used. Nonlimiting examples of the extraction solvent include water, alcohols or mixed solvents thereof. When alcohol is used as a solvent, C ₁ to C ₄ alcohols may be more preferably used. The fermented product of the present invention or the extract of the aged product may be purified water, butylene glycol, 1,2-hexanediol or a mixed solvent thereof.

As used herein, the term "collagen" is a protein which occupies the largest quantitative and functionally in the human body. It is an extracellular matrix that forms the skeleton of all cells in the human body, more specifically connective tissue). Human tissues containing large amounts of collagen include bone tissues such as skin, bones and cartilage, tendons, and ligaments. It is known that aging of the tissues causes human aging diseases such as skin wrinkles and sagging and osteoporosis, which is caused by a decrease in collagen amount and function due to decrease of collagen synthesis of tissues.

On the other hand, the main function of dermal fibroblasts is extracellular matrix synthesis and regeneration of injured skin tissue through proliferation. The extrinsic aging factors such as photoaging, accumulation of damage due to free oxygen, and endogenous Aging factors reduce the physiological activity of dermal fibroblasts in the dermal layer. Type I collagen, which accounts for more than 95% of the extracellular matrix and plays a major role in the physiological activity of fibroblasts through interactions and signal interactions with adhesion molecules and cytoskeletons of cells, Is reduced, the amount of collagen in the skin tissue is reduced, whereby the surface tension is reduced, so that the skin elasticity is lowered and the skin wrinkles are formed, and the physiological activity including cell proliferation and regeneration is decreased This causes a vicious circle. Therefore, collagen of skin fibroblasts may be directly related to restoration or regeneration of tissue during skin regeneration, skin elasticity, wrinkle formation and skin damage.

That is, when the synthesis of collagen or procollagen of dermal fibroblast is promoted, improvement of skin elasticity, skin regeneration, improvement of skin wrinkles, wound healing, restoration and regeneration of damaged skin tissue, And prevention of skin aging can be obtained.

Therefore, the grape fruit, stem and flower fermented product of the present invention; Aging of the fermentation product; Or the extract of the fermented product or the aged product promotes collagen synthesis, thereby increasing skin elasticity, regenerating skin, improving skin wrinkles, healing wounds, restoring and regenerating damaged skin tissue, And prevention of skin aging and the like. Preferably, it may have the effect of improving skin elasticity, improving skin wrinkles and regenerating skin.

Specifically, in one experimental example of the present invention, the grape extract fermented and aged only grape fruit was not able to confirm the collagen aggregation performance. However, the grape extract, which fermented and fermented together with grape fruit, stem and flower hull, (Table 1). That is, fermentation and aging grape extract of grape fruit, stem and flower hull fermented and ripened grape fruit; And the fermented grape extract, which had not undergone the fermentation process, had remarkable collagen synthesis performance.

In the composition for promoting collagen synthesis according to the present invention, fermented products of grape fruit, stem and flower hull; Aging of the fermentation product; Or the extract of the fermented product or the aged product is used in an amount of 0.01 to 90% by weight, preferably 0.1 to 80% by weight, based on the whole composition. When the content is more in the composition, the improvement effect on the dermal density and the skin fatigue is improved. However, if the content is less than 0.01% by weight, a substantial effect is hardly expected. When the content exceeds 90% by weight, And it is undesirable because it affects stability of cosmetics and the like.

In another aspect, the present invention provides a method of producing a grape fruit comprising the steps of: (a) fermenting grape fruit, stem and hibiscus;

(b) aging the fermentation product obtained in step (a); And

(c) extracting the fermentation product obtained in the step (a) or the aged product obtained in the step (b).

Preferably, the step (c) may be a step of extracting the aged material obtained in step (b).

In the above production method of the present invention, the description of each of the above steps is the same as described above in connection with the composition of the present invention.

In another aspect, the present invention provides a cosmetic composition for skin elasticity or wrinkle improvement or skin regeneration comprising the composition for promoting collagen synthesis as an active ingredient.

In the present invention, the term "skin elasticity" is represented by elastic fibers composed of elastin existing in the dermal layer. Such elastic fibers have a very low elastic modulus such as rubber and are easily deformed by a small force. When the force is removed, it easily returns to the original shape. In addition, the elastic fibers have a form in which microfibrils are embedded in an amorphous matrix called elastin, and elastin is a protein that is found only in elastic fibers such as desmosine and isodesmosine derived from lysine, It is a protein composed of one amino acid. These desmosins and isodesmesynes form cross-links in long peptide chains, which makes elastin behave like a rubber.

In the present invention, the term "skin elasticity improvement" means that elastic fibers composed of elastin are present together with collagen fibers, and elastic elasticity is maintained or increased in a state where elastin and collagen are sufficiently present.

In the present invention, the term "skin wrinkle " means a skin formed by decay of skin, which may be caused by a cause of a gene, a decrease in collagen existing in the skin dermis, or an external environment.

In the present invention, "improvement of skin wrinkles" refers to inhibiting or inhibiting the generation of wrinkles on the skin, or alleviating already formed wrinkles.

The term "skin regeneration" in the present invention refers to a process of restoring skin tissue against skin (cell) damage caused by external and internal causes of the skin. Damage due to external causes may include ultraviolet rays, external contaminants, wound, trauma, etc. The damage caused by the internal causes may be stress.

Accordingly, the cosmetic composition of the present invention promotes the synthesis of collagen or procollagen, thereby improving skin elasticity, regenerating skin, improving skin wrinkles, healing wounds, restoring and regenerating damaged skin tissue, And prevention of skin aging and the like.

Specifically, in one embodiment of the present invention, an underwater type emulsion was prepared by fermenting both grape fruit, stem and flower hull, aging for 2 years, and extracting the fermented and aged grape extracts in an amount to prepare an underwater type emulsion (Examples 1 to 3). In addition, as a result of skin safety evaluation of the cosmetic compositions of Examples 1 to 3, it was found that the composition was a safe sample which does not cause skin irritation (Table 5), and the dermal tightness improvement evaluation result shows that the composition has improved dermal density (Table 6).

That is, the composition comprising the fermented and aged grape extract according to the present invention as an active ingredient effectively promoted collagen synthesis without affecting the formulation stability and little skin irritation, and it was confirmed that the skin elasticity improvement and skin regeneration effect were excellent .

The formulation of the cosmetic composition of the present invention is not particularly limited and may be appropriately selected according to the purpose. For example, the cosmetic composition of the present invention can be manufactured by a formulation such as softening water, nutritional lotion, nutritional cream, massage cream, essence, pack, emulsion, oil gel and the like.

In addition, the cosmetic composition of the present invention may further comprise at least one cosmetically acceptable carrier incorporated in a cosmetic composition for general skin, and examples thereof include oil, water, a surfactant, a moisturizer, a lower alcohol, A thickening agent, a chelating agent, a coloring matter, an antiseptic, a perfume, and the like may be appropriately compounded, but the present invention is not limited thereto.

The cosmetically acceptable carrier contained in the cosmetic composition of the present invention varies depending on the formulation of the cosmetic composition.

In addition to the active ingredient of the present invention, the number of softening times may be 1 to 50% by weight of polyhydric alcohols (glycerin, butyleneglycol, propylene glycol, propanediol, etc.) and a surfactant (polyethylene oleyl ether, polyoxyethylene hardened castor oil Etc.) in an amount of 0.05 to 5% by weight.

The nutritional lotion and nutritional cream may contain, in addition to the active ingredient of the present invention, oils (such as hydrocarbons such as squalane, ester oils such as caprylic / capric triglyceride and octyldodecanol, natural oils such as olive oil, 1 to 40% by weight of a wax component (cetyl alcohol, stearyl alcohol, cetostearyl alcohol, behenyl alcohol, beeswax, ceresin, shea butter, etc.) % ≪ / RTI > by weight. The essence may be prepared so as to contain 5 to 30% by weight of polyhydric alcohols in addition to the active ingredient of the present invention. In addition to the fermented product of the present invention, the massage cream may be prepared so as to contain 30 to 70% by weight of an oil such as mineral oil, isononylisononanoate and the like. The pack may contain, in addition to the active ingredient of the present invention, kaolin, talc, zinc oxide, titanium dioxide and the like in addition to the active ingredient of the present invention in peel off packs or general emulsifiable cosmetics containing 5 to 20% by weight of polyvinyl alcohol And a wash off pack containing 5 to 40% by weight of pigment.

In addition, the cosmetic composition of the present invention may contain, in addition to the components described above, an oil, a water, a surfactant, a moisturizer, a lower alcohol, a thickener, a chelating agent, a pigment, a preservative, Can be used.

Specifically, as a result of evaluation of the formulation stability of the cosmetic compositions of Examples 1 to 3, precipitation and separation phenomena were observed at a low temperature (0 ° C, freezing and thawing) and a high temperature (40 ° C, 50 ° C) Fermented and fermented grape extracts did not interfere with the formulation stability.

In another aspect, the present invention provides a food composition for skin elasticity or wrinkle improvement or skin regeneration comprising the composition for promoting collagen synthesis as an active ingredient.

The food composition of the present invention is not particularly limited and may include a health functional food composition.

When the health functional food composition of the present invention is used as a food additive, the composition may be added as it is or may be used together with other food or food ingredients, and may be appropriately used according to a conventional method.

The kind of the food is not particularly limited, and includes food in a conventional sense. Non-limiting examples of the food to which the substance can be added include meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gums, dairy products including ice creams, , A drink, an alcoholic beverage, and a vitamin complex.

When the health functional food composition of the present invention is a beverage composition, various flavoring agents, natural carbohydrates, and the like may be contained as additional components such as ordinary beverages. Non-limiting examples of such natural carbohydrates include monosaccharides such as glucose and fructose; Disaccharides such as maltose and sucrose; Natural sweetening agents such as dextrin, cyclodextrin; Synthetic sweetening agents such as saccharin and aspartame, and the like. The proportion of the additional component added may be appropriately determined by a person skilled in the art.

In addition to the above, the health functional food composition of the present invention may contain various nutrients, vitamins, electrolytes, flavors, colorants, pectic acid and salts thereof, alginic acid and salts thereof, organic acids, protective colloid thickening agents, pH adjusting agents, stabilizers, , Alcohols, carbonating agents used in carbonated drinks, and the like. In addition, the health functional food composition of the present invention may contain pulp for the production of natural fruit juice, fruit drink or vegetable drink. These components may be used independently or in combination of two or more. The ratios of these additives can also be suitably selected by those skilled in the art.

In another aspect, the present invention provides a pharmaceutical composition for skin elasticity or wrinkle improvement or skin regeneration comprising the composition for promoting collagen synthesis as an active ingredient.

As used herein, the terms "improvement of skin elasticity "," improvement of skin wrinkles ", or "skin regeneration"

The pharmaceutical composition of the present invention may further comprise a pharmaceutically acceptable carrier in addition to the composition for promoting collagen synthesis as an effective ingredient.

In the present invention, the above-mentioned "pharmaceutically acceptable" means that it is commonly used in the pharmaceutical field, which does not disturb the biological activity and properties of the compound administered, without irritating the organism upon administration thereof.

The pharmaceutical composition of the present invention may be formulated together with the carrier to be used as medicines, feed additives, drinking water additives, and the like.

In the present invention, the type of the carrier is not particularly limited and any carrier conventionally used in the art can be used. Examples of the carrier include, but are not limited to, saline, sterilized water, Ringer's solution, buffered saline, albumin injection solution, lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, maltodextrin, glycerol, . These may be used alone or in combination of two or more.

In addition, the pharmaceutical composition of the present invention may be supplemented with other pharmaceutically acceptable additives such as excipients, diluents, antioxidants, buffers or bacteriostats, and may be used as fillers, extenders, wetting agents, disintegrants, , A binder, a lubricant, and the like may be additionally used.

The pharmaceutical composition of the present invention may be formulated into various formulations suitable for oral administration or parenteral administration.

Non-limiting examples of such oral dosage forms include troches, lozenges, tablets, aqueous suspensions, oily suspensions, prepared powders, granules, emulsions, hard capsules, soft capsules, syrups or elixirs .

In order to formulate the pharmaceutical composition of the present invention for oral administration, a binder such as lactose, saccharose, sorbitol, mannitol, starch, amylopectin, cellulose, or gelatin; Excipients such as dicalcium phosphate and the like; Disintegrating agents such as corn starch or sweet potato starch; Lubricants such as magnesium stearate, calcium stearate, sodium stearyl fumarate or polyethylene glycol wax may be used, and sweeteners, fragrances, syrups and the like may also be used. .

Furthermore, in the case of capsules, in addition to the above-mentioned substances, liquid carriers such as fatty oils can be further used.

Non-limiting examples of the parenteral preparation include injections, suppositories, respiratory inhalation powders, aerosol preparations for spraying, ointments, powder for application, oils, creams and the like.

In order to formulate the pharmaceutical composition of the present invention for parenteral administration, sterilized aqueous solutions, non-aqueous solvents, suspensions, emulsions, freeze-dried preparations and external preparations may be used. Examples of the non-aqueous solutions and suspensions include propylene glycol, polyethylene Glycerol, vegetable oils such as olive oil, injectable esters such as ethyl oleate, and the like.

More specifically, when the pharmaceutical composition of the present invention is formulated into an injection, the composition of the present invention is mixed with water or a stabilizer or buffer in water to prepare a solution or suspension, which is then mixed with an ampoule or a vial Unit dosage form. In addition, when the pharmaceutical composition of the present invention is formulated into an aerosol formulation, a propellant or the like may be added together with the additive such that the water-dispersed concentrate or the wet powder is dispersed.

When the pharmaceutical composition of the present invention is formulated into an ointment, cream, or the like, it is possible to use an animal oil, a vegetable oil, a wax, a paraffin, a starch, a tracer, a cellulose derivative, a polyethylene glycol, a silicone, a bentonite, Or the like can be used as a carrier.

The pharmaceutically effective amount and the effective dose of the pharmaceutical composition of the present invention may be varied depending on the formulation method, administration method, administration time and / or route of administration of the pharmaceutical composition, Including, but not limited to, the type and severity of the disease, the type of subject being treated, the age, body weight, general health, severity or severity of the disease, sex, diet, excretion, Can be varied according to various factors and similar factors well known in the medical field, and those skilled in the art can easily determine and prescribe effective dosages for the desired treatment.

The dose for a more preferable effect of the pharmaceutical composition of the present invention may be preferably 1 mg / kg to 100,000 mg / kg per day, more preferably 10 mg / kg to 10,000 mg / kg per day. The pharmaceutical composition of the present invention may be administered once a day or divided into several doses. Accordingly, the dosage is not limited in any way to the scope of the present invention.

The route of administration and the mode of administration of the pharmaceutical composition of the present invention may be independent of each other, and the method is not particularly limited, and any route of administration and administration method may be used as long as the pharmaceutical composition can reach the desired site . The pharmaceutical composition may be administered orally or parenterally.

The parenteral administration may be, for example, intravenous administration, intraperitoneal administration, intramuscular administration, transdermal administration or subcutaneous administration, and a method of applying, spraying or inhalation the composition to a diseased site may also be used But are not limited to.

In another embodiment, the present invention provides a quasi-drug composition for skin elasticity or wrinkle improvement or skin regeneration comprising the composition for promoting collagen synthesis as an effective ingredient.

The quasi-drug composition of the present invention can be used as a body cleanser, a disinfectant cleaner, a detergent, a kitchen detergent, a cleaning detergent, a toothpaste, a gauze agent, a wet tissue, a detergent, a soap, a hand wash, a hair cleanser, a hair softener, a humidifier filler, But are not limited thereto.

The composition for accelerating collagen synthesis comprising the fermented and aged grape extract according to the present invention as an active ingredient effectively promotes collagen synthesis without substantially affecting the stability of the formulation and has little skin irritation to improve skin elasticity, The effect is excellent.

Hereinafter, embodiments of the present invention will be described in detail to facilitate understanding of the present invention. However, the embodiments according to the present invention can be modified into various other forms, and the scope of the present invention should not be construed as being limited to the following embodiments. Embodiments of the invention are provided to more fully describe the present invention to those skilled in the art.

Manufacturing example  1: fermentation and aging grape extract and fermented grape extract

The fermented and aged grape extracts and fermented grape extracts used in Examples and Experimental Examples of the present invention are as follows.

Fermented and ripened grape extracts were prepared using either grape fruit, stem and calyx, or using only grape fruit. Grape fruit, stem and horseradish or grape fruit only, and after inoculation with Saccharomyces bacterium, they were fermented to obtain a fermented product, which was then aged for 2 years in an oak barrel. After two years, a solution such as purified water, butyleneglycol or 1,2-hexanediol was added to the fermented and aged solution, and the mixture was stirred to extract an active ingredient, followed by filtration to produce a fermented and aged grape extract.

The fermented grape extract is obtained by sterilizing only grape fruit and then inoculating Saccharomycetes to obtain a fermented product. After the fermentation, the fermented product is treated with purified water, butylene glycol or 1,2-hexanediol Solvent was added and stirred to extract an active ingredient, followed by filtration.

Experimental Example  1: Cytotoxicity experiment

In order to verify the primary safety as a raw material used in cosmetics, fermentation of grape fruit, stem and flower hull, fermentation for 2 years and fermentation and ripening grape extract obtained by extraction were added to V79-4 cells (Chinese hamster, lung tissue (Mossman T. (1983) Rapid colorimetric assay for cellular growth & survival: application to proliferation & cytotoxicity assays. Journal of Immunological Methods 65, 55-63). The cytotoxicity test results are shown in Table 1 below.

sample IC ₅₀ (%, w / v) Sodium lauryl sulfate (SLS) 0.005 Squalane 0.05 Fermented and ripened grape extract 1.0

IC ₅₀ : Inhibitory Concentration 50, 50% of the cell killing concentration

As can be seen from Table 1, the IC ₅₀ value of the fermented and aged grape extracts was over 1.0, which was lower than that of sodium lauryl sulfate by a factor of 200 or more, and the cell toxicity was 20 times higher than that of squalane Toxicity was low and the fermented and aged grape extract of the present invention was found to have excellent safety.

Experimental Example  2: Collagen Sum performance  Experiment

Since the collagen is synthesized as a procollagen in a cell and secreted outside the cell to polymerize the collagen fiber, the extract prepared in Preparation Example 1 is added to a culture medium of human fibroblasts derived from human, Collagen synthesis effect. After the extract was applied to a PICP EIA kit (Procollagen Type I C-Peptide Enzyme Immunoassay Kit), the amount of procollagen type I protein (PIP) was measured to confirm the collagen synthesis performance. Table 2 shows the increase in the PIP value after applying the extract with the PIP value of the extract not coated being 100%.

PIP (% of control) Extract concentration (v / v,%) 0.0 0.1 0.5 One Fermentation and 2 year ripening grape extract (%)
(Grape fruit, stem, flower fermentation) 100.0 105.0 109.8 118.7 Fermentation and 2 year ripening grape extract (%)
(Grape fruit fermentation) 100.0 98.6 99.6 103.8 Fermented grape extract (%)
(Grape fruit fermentation) 100.0 94.8 95.6 94.6

As can be seen from Table 2, the grape extract fermented and aged only grape fruit was not different from the grape extract not coated with sample, and the collagen aggregation performance of the extract could not be confirmed. In contrast, grape extract, which was fermented and aged together with grape fruit, stem and flower hull, increased the ability of collagen synthesis according to the concentration applied to the kit.

In other words, it was found that the grape fruit, stem and flower fermentation and aging grape extract had remarkable collagen synthesis ability compared with fermented and fermented grape extract and fermented grape extract without aging process.

Example  1 to 3 and Comparative Example  1 to 2: Cosmetics  Preparation and formulation safety evaluation of composition

According to the composition shown in Table 3 below, Examples 1 to 3 are water-in-water emulsions prepared by fermenting all grape fruit, stem, and hymen after fermentation for 2 years, and fermenting and ripening grape extracts in various amounts. Comparative Example 1 is a comparative sample containing no grape extract, and Comparative Example 2 is an underwater type emulsion containing an extract obtained by fermenting only grape fruit and not aging.

The underwater type emulsion was prepared according to the following method. First, the water phase and the oil phase were heated and dissolved at 70 to 75 DEG C, respectively. The oil phase was added to the water phase, and the emulsion was emulsified for 8 minutes under the condition of a homomixer at 6000 rpm. Or fermented grape extract and flavor were added thereto, and the resulting mixture was mixed for 3 minutes under the condition of a homomixer at 6000 rpm and cooled to 30 ° C to prepare an underwater type emulsion.

Example 1 Example 2 Example 3 Comparative Example 1 Comparative Example 2 Awards Purified water To 100 To 100 To 100 To 100 To 100 Tromethamine 0.15 0.15 0.15 0.15 0.15 Carboxyvinyl polymer
(1.0 wt% solution) 15.00 15.00 15.00 15.00 15.00 glycerin 7.00 7.00 7.00 7.00 7.00 Tree sodium 0.04 0.04 0.04 0.04 0.04 Paid Squalane 3.00 3.00 3.00 3.00 3.00 Triethanolhexanone 2.00 2.00 2.00 2.00 2.00 Cetearyl alcohol,
Cetearyl glucoside 1.00 1.00 1.00 1.00 1.00 C12-16 alcohol,
Hydrogenated lecithin,
Palmitic acid 0.50 0.50 0.50 0.50 0.50 Other Spices Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Fermentation and 2-year ripening grape extract
(Grape fruit, stem, flower) 0.10 1.00 10.00 - - Fermented grape extract
(Grape fruit) - - - - 1.00

As a result of evaluation of stability for 30 days after the preparation of the cosmetic compositions of Examples 1 to 3 in Table 3, precipitation and separation phenomenon were not observed at low temperature (0 DEG C, freezing and thawing) and high temperature (40 DEG C, 50 DEG C) It was judged that the ripened grape extract did not inhibit the formulation stability.

Experimental Example  3: Skin safety evaluation

About 0.2 g of each of the cosmetic compositions of Examples 1 to 3 and Comparative Examples 1 and 2 was applied to the arms of 50 healthy adults. After 2 hours had elapsed from the removal of the pin chamber, the change of the skin condition was visually inspected based on the criteria in Table 4, and the skin irritation index was converted into Equation 1. The final skin stability results are shown in Table 5 below.

Irritation degree
(Score) Stimulus response
(Reaction) Criteria 0 - No symptoms One + - If there is only a slight erythema reaction 2 + Clear erythema, edema, but no blisters 3 ++ If you have a clear erythema, edema, blisters 4 +++ When there is a logarithm

[Equation 1]

Skin irritation index = irritation degree x number of subjects to be tested / total number of subjects to be tested

division Example 1 Example 2 Example 3 Comparative Example 1 Comparative Example 2 Stimulation index 0.05 0.02 0.01 0.11 0.09

As can be seen from Table 5, the cosmetic compositions of Examples 1 to 3 exhibited a lower irritation index than Comparative Examples 1 and 2. An irritation index of 0.05 or less means that the skin shows little irritation. Thus, it was found that the cosmetic composition of the present invention is a safe sample which does not cause skin irritation.

Experimental Example  4 : Dermal density  Improvement effect

Dermal density was measured by using Dermascan-C, a device for imaging high-resolution ultrasonic waves, using the cosmetic compositions of Examples 1 to 3 and Comparative Examples 1 and 2 on the ball area, and then measuring the dermal density Were measured. The range of analysis was defined as the subcutaneous fat layer just below the epidermis, and the intensity, which is the density of the dermal density, was measured and analyzed. As the density of dermis increases, the measured value becomes higher. When the density decreases, the measured value becomes lower. Table 6 shows the results of calculating the dermal density increase rate using equation (2).

&Quot; (2) "

Growth rate (%) = (measured value after use of sample - measured value before use of sample) / (measured value before use of sample) X 100

Density increase rate of dermis (%) After 2 weeks of use (%) After 4 weeks of use (%) Example 1 1.08 2.45 Example 2 3.24 5.81 Example 3 6.02 8.43 Comparative Example 1 0.00 0.00 Comparative Example 2 0.12 0.19

As shown in Table 6, when the fermented and fermented grape extracts were contained, the dermal density increased significantly at 2 weeks and 4 weeks of use. Therefore, it was found that fermentation and aging grape extracts effectively help to improve dermal density.

Therefore, it was confirmed that the fermented and aged grape extract of the present invention effectively promoted collagen synthesis and showed improvement in dermal density, and it was found that the extract had improved skin elasticity, wrinkle and skin regeneration .

Experimental Example  5: Skin fatigue improvement effect

Skin fatigue of the ball area was measured with Cutometer MPA580 after 2 weeks and 4 weeks of using the cosmetic composition of Examples 1 to 3 and Comparative Examples 1 and 2. Cutometer The MPA580 measures skin fatigue using the principle that when the skin is sucked into the probe for a measuring time and then the sound pressure is removed, the skin is restored to its original shape. The MPA580 has a constant sound pressure of 400 mbar and a suction time of 1 second And a relaxation time of 1 second. The measurement results were obtained by calculating the arithmetic mean, and the reduction rate was calculated from the equation (3).

&Quot; (3) "

Decrease rate (%) = (Measured value before sample use - Measured value after using sample) / (Measured value before use of sample) X 100

Skin fatigue reduction rate (%) After 2 weeks of use After 4 weeks of use Example 1 3.84 4.11 Example 2 4.37 6.40 Example 3 5.97 7.54 Comparative Example 1 0.00 0.00 Comparative Example 2 0.81 0.98

As shown in Table 7, skin fatigue was statistically significantly decreased when fermented and ripened grape extract was used. Therefore, fermentation and ripening grape extract are effective for improving skin fatigue.

Accordingly, it was confirmed that the fermented and aged grape extract of the present invention effectively promotes collagen synthesis and exhibits skin fatigue improving effect. As a result, it was found that the extract has improved skin elasticity, wrinkle and skin regeneration.

From the above description, it will be understood by those skilled in the art that the present invention may be embodied in other specific forms without departing from the spirit or essential characteristics thereof. In this regard, it should be understood that the above-described embodiments are to be considered in all respects as illustrative and not restrictive. The scope of the present invention should be construed as being included in the scope of the present invention without departing from the scope of the present invention as defined by the appended claims.

Claims (8)

Grape fruit, stem and flower fermented; Aging of the fermentation product; Or an extract of said fermented product or aged product as an active ingredient.
The method according to claim 1,
The fermented product is saccharide as MY access (Saccharomyces), A mound Ras Fora (Torulaspora) inside, Xi Kosaka in my process (Zygosaccharomyces) genus or Lactobacillus bacteria (Lactobacillus) in the composition for collagen synthesis, characterized in that the fermentation by bacteria to promote .
The method according to claim 1,
Wherein the aged product is prepared by aging the fermented product for 2 years or more.
The method according to claim 1,
Wherein the extract is obtained by extracting the fermented product or the aged product with purified water, butylene glycol, 1,2-hexanediol, or a mixed solvent thereof.
The method according to claim 1,
The fermented product, the aged product of the fermented product; Or an extract of the fermented product or the aged product in an amount of 0.01 to 90% by weight based on the total weight of the composition.
(a) fermenting grape fruit, stem and flower buds;
(b) aging the fermentation product obtained in step (a); And
(c) extracting the aged material obtained in the step (b).
A cosmetic composition for skin elasticity or wrinkle improvement or skin regeneration comprising the composition of any one of claims 1 to 5 as an active ingredient.
A quasi-skin composition for skin elasticity or wrinkle improvement or skin regeneration comprising the composition of any one of claims 1 to 5 as an active ingredient.