KR102133473B1 - A composition as a prebiotic for improving intestinal microflora containing sweet potato vines - Google Patents

Abstract

The present invention relates to a prebiotic composition, a composition for improving intestinal micro-organisms, a composition for improving intestinal health or intestinal function, by containing a sweet potato stem extract or a sweet potato stem polymer fraction as an active ingredient, thereby improving intestinal health or intestinal function. By inducing the growth of beneficial bacteria in the intestine to improve, intestinal health or intestinal function can be improved, prevented or treated. Also, since it is non-toxic, it can be consumed in the form of food.

Description

A composition as a prebiotic for improving intestinal microflora containing sweet potato vines}

The present invention relates to a composition for improving intestinal microflora, and by containing a sweet potato stem extract or a sweet potato stem polymer fraction as an active ingredient, it induces proliferation of beneficial bacteria in the intestine, and relates to a composition capable of improving and preventing intestinal health or intestinal function. .

Prebiotics (prebiotics) is a non-digestible food additive that has beneficial effects on the host by limiting gut microbes or selectively promoting the growth of useful microorganisms. Chitin oligosaccharides) and dietary fiber (dextrin, inulin, gum, etc.).

Within the human intestine, more than 400 species of bacteria inhabit a number of about 100 trillion per gram of intestinal contents and consist of a complex ecosystem that maintains a constant balance. Intestinal flora can be divided into beneficial and harmful bacteria, and their health is controlled by their balance. In order to maintain health, it is necessary to maintain the intestinal microflora with many beneficial bacteria and fewer harmful bacteria. The microflora can be changed according to the state of the human body, such as food, stress, hormone secretion, etc., and the enzymes and metabolites they produce are involved in the metabolism of various substances in the body, absorption of nutrients, drugs, and toxic substances Affects the creation of Accordingly, the need for the development of a method capable of promoting the growth of beneficial bacteria in the gut and suppressing harmful bacteria is gradually increasing.

Sweet potatoes, also known as sweet potatoes and sweet potatoes, are edible crops that are widely cultivated throughout Korea. It is about 3m long, the leaves are misaligned, the leaf body is shallowly divided into a heart shape, and when the leaves and stems are cut, juice comes out, and the roots of the petiole at the bottom of the stem take root, and a part of them grows in the ground to become sweet potatoes that are tubers. The shape varies from a pointed cylindrical shape to a ball shape, and the colors vary from white, yellow, light red, red, and light purple. In particular, the stems take root along the long ground. Sweet potato stems are round and have almost no hair, and there are many more. The tip has a lot of curls at the base and is pale and is divided into erect and dobok type according to its growth habit.

Sweet potato contains 68.5% of moisture, 26.4% of carbohydrates, 1.8% of protein, and 0.6% of fat, and contains a lot of carotene, provitamin A, and vitamin B1, B2, C, and niacin. In particular, it contains a large amount of carbohydrates, so it can be used as a stock substitute, and has been cultivated as an old crop. Sliced and dried raw sweet potatoes are called <sweet potato> and are convenient for storage and used as a raw material for alcohol. During storage, moisture decreases, and starch is sweetened by the action of enzymes. Sweet potato is mainly composed of starch and sugar, and is low in protein and fat. It is cooked by steaming, baking, frying, etc. It is also used as a material or feed for starch, starch syrup, sweets, alcohol, alcohol, and various chemicals. Leaves and stems are also used as feed and green manure.

Domestic patents using such sweet potato stems are mainly made of healthy foods such as sweets, tea, and animal feeds made of sweet potato stems, and there is no known prebiotic composition capable of effectively improving the total microorganisms in the gut. There is no bar.

Therefore, the present inventors made great efforts to develop a new prebiotic that can effectively improve the total bacteria of intestinal microorganisms from natural products, and as a result, the present inventors promoted the growth of beneficial bacteria in the gut stem stem or sweet potato stem polymer fraction. By confirming that it has the activity of inhibiting the growth of harmful bacteria in the intestine, the present invention has been completed.

Republic of Korea Patent Publication No. 10-2002-0071422

An object of the present invention is to provide a composition for improving intestinal health or bowel function and a prebiotic composition using sweet potato stem extract as an active ingredient.

In addition, another object of the present invention is to provide a prebiotic composition and a composition for improving intestinal health or intestinal function, comprising a sweet potato stem polymer fraction as an active ingredient.

In order to achieve the above object, the present invention provides a prebiotic composition using sweet potato stem extract as an active ingredient.

The sweet potato stem extract may be extracted with water, alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof.

The prebiotic composition may be to promote the growth of beneficial bacteria in the intestine.

The beneficial bacteria in the intestine may be a strain of Bifidobacterium genus, Lactobacillus genus, or Streptococcus genus.

In addition, in order to achieve the above other object, the present invention provides a prebiotic composition comprising a sweet potato stem polymer fraction as an active ingredient.

The sweet potato stem polymer fraction may be a fraction of sweet potato stem extract having a molecular weight of 10 kDa or more.

The polymer fraction is (a) precipitating the sweet potato stem with water, C1 to C4 alcohol or a mixed solvent thereof; And (b) recovering a polymer fraction having a molecular weight of 10 kDa or higher using ultrafiltration or gel filtration chromatography from the extract.

In addition, in order to achieve the above object, the present invention provides a composition for improving intestinal health or intestinal function using sweet potato stem extract as an active ingredient.

The sweet potato stem extract may be extracted with water, alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof.

In addition, in order to achieve the other object, the present invention provides a composition for improving intestinal health or intestinal function, comprising a sweet potato stem polymer fraction as an active ingredient.

The sweet potato stem polymer fraction may be a fraction of sweet potato stem extract having a molecular weight of 10 kDa or more.

The polymer fraction is (a) precipitating the sweet potato stem with water, C1 to C4 alcohol or a mixed solvent thereof; And (b) recovering a polymer fraction having a molecular weight of 10 kDa or higher using ultrafiltration or gel filtration chromatography from the extract.

것일 수 있다.The sweet potato stem polymer fraction does not contain inert dietary fiber compared to the whole polymer fraction

May be

The intestinal microbial improvement or prebiotic composition of the present invention has an effect of promoting the growth of beneficial bacteria in the intestine, even though it does not contain an inactive dietary fiber known as an active ingredient of sweet potato stems. Through the significant enhancement effect of the beneficial bacteria in the intestine, it is possible to improve, suppress, and prevent intestinal infection by pathogenic bacteria, and maintain the balance of the intestinal flora. That is, the prebiotic composition of the present invention can be utilized for improving intestinal microbes, intestinal flora, and intestinal environment, and can be consumed in the form of food because it is non-toxic.

1 is a graph showing the analysis of a prebiotic activity score in the intestine when a sweet potato stem hydrothermal extract of Example 1 is treated with various bacteria, FOS (fructo oligosaccharide). 1A is for Lactobacillus paracasei ATCC 25302(T) (Lactobacillus paracasei subsp. tolerans), FIG. 1B is Lactobacillus plantarum, FIG. 1C is Lactococcus lactis NCDO604(T) ( Lactococcus lactis subsp.lactis), Figure 1D is for Bifidobacterium longum, Figure 1E is for Bifidobacterium bifidum, Figure 1F is for Bifidobacterium breve .
2 is a graph showing the analysis of the prebiotic activity score in the intestine when the sweet potato stem polymer fraction of Example 2, FOS (fructo oligosaccharide) is treated with various bacteria. 2A is for Lactobacillus paracasei ATCC 25302(T) (Lactobacillus paracasei subsp. tolerans), FIG. 2B is Lactobacillus plantarum, FIG. 2C is Lactococcus lactis NCDO604(T) ( For Lactococcus lactis subsp.lactis, Figure 2D is Bifidobacterium longum, Figure 2E is Bifidobacterium bifidum, Figure 2F is Bifidobacterium breve It is about.
Figure 3 is a graph showing the analysis of the activity (prebiotic activity score) for Lactobacillus and Lactococcus among the beneficial bacteria in the intestine when the sweet potato stem hydrothermal extract of Example 1, FOS (fructo oligosaccharide) was treated with the total bacteria ) And the sweet potato stem polymer fraction of Example 2, when treated with FOS (fructo oligosaccharide), is a graph (B) showing the activity (prebiotic activity score) of lactobacilli and lactococcus among the beneficial bacteria in the intestine.
4 is a graph showing the analysis of the prebiotic activity score (prebiotic activity score) in the intestinal tract when the sweet potato stem hydrothermal extract of Example 1, FOS (fructo oligosaccharide) is treated with total bacteria, and the sweet potato stem polymer fraction of Example 2, FOS When (Fracto oligosaccharide) is treated, it is a graph showing an analysis of the prebiotic activity score in the intestine. Figure 4A shows the average of the intestinal beneficial bacteria activity (prebiotic activity score) for the total bacteria of the sweet potato stem hydrothermal extract of Example 1, Figure 4B is the intestinal beneficial bacteria activity for the total bacteria of the sweet potato stem polymer fraction of Example 2 ( prebiotic activity score).

The present invention relates to a composition for improving intestinal health or bowel function and a probiotic composition that activates proliferation of beneficial bacteria in the intestine by containing a sweet potato stem extract or a sweet potato stem polymer fraction as an active ingredient.

Hereinafter, the present invention will be described in detail.

The present invention relates to a prebiotic composition using the sweet potato stem extract as an active ingredient.

In the present invention,'prebiotic or prebiotic' is a food component that is not easily digested by the body, and does not undergo hydrolysis or absorption at the upper part of the gastrointestinal tract, and stimulates the growth or activity of certain symbiotic bacteria in the intestinal tract. It is a substance that induces in a direction beneficial to the health of the host. Specifically, it means a substance that promotes the growth of beneficial bacteria in the intestine. The prebiotic composition may also be referred to as a composition for improving intestinal microflora or microflora. This increases or decreases the efficacy of probiotics and improves intestinal health or improves intestinal function. Improving intestinal microflora or intestinal microflora may mean promoting the growth of beneficial bacteria in the intestine and suppressing the growth of harmful bacteria in the intestine, but maintaining the balance of beneficial and harmful bacteria in the intestine.

In the present invention, the'extract' includes not only the crude extract obtained by treating the extraction raw material with the extraction solvent, but also the processed product of the crude extract. For example, sweet potato stem extract may be prepared in powder form by additional processes such as distillation under reduced pressure and freeze drying or spray drying.

In addition, in the present invention, the'sweet potato stem extract' is an extract of the sweet potato stem using water, an alcohol having 1 to 4 carbon atoms or a mixed solvent thereof, and the extraction method is not particularly limited. Preferably, the sweet potato stem extract may be a sweet potato stem hot water extract or an aqueous sweet potato stem ethanol solution, for example, a sweet potato stem alcohol extract.

In addition, the sweet potato stem extract may be a process of extracting the sweet potato stem, and repeatedly extracting it once or more with water, alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof.

The sweet potato stem extract is an extract that does not contain a component known to have an effect of improving intestinal health or intestinal function, such as an inactive dietary fiber present in the sweet potato stem, and nonetheless, promotes the growth of beneficial bacteria in the intestine, It is characterized by exhibiting the effect of improving bowel health or bowel function.

The sweet potato stem may be cut into a certain size, dried using a shade or a dryer, and then crushed or used as it is. The drying period is not particularly limited as long as the moisture content in the sweet potato stem is less than 20%.

In the present invention,'as an active ingredient' or'comprising as an active ingredient' means that the sweet potato stem extract of the present invention contains an amount sufficient to achieve the efficacy or activity of promoting the growth of beneficial bacteria in the intestine.

The term intestinal beneficial bacteria, which is a term used herein, means a beneficial bacteria that inhabit the intestine of the host and a strain that has a beneficial effect on the intestinal environment when the intestine is reached.

These are bacteria that survive the stomach and bile acids, reach the small intestine, multiply and settle in the intestine, exhibit useful effects in the intestine, and are non-toxic and satisfy non-pathogenic conditions. That is, it can be understood as probiotics.

In the present invention, when treating a composition containing the sweet potato stem extract as an active ingredient, it was confirmed that the composition has an effect of improving intestinal flora.

The beneficial bacteria in the intestine may be strains of the genus Bifidobacterium, genus Leukonostock, genus Pediococcus, genus Weisella, streptococcus, genus Lactobacillus, and genus Lactococcus, but are not particularly limited thereto, preferably Lactobacillus paracasei subsp. tolerans, Lactobacillus plantarum, Lactococcus lactis subsp. lactis, Bifidobacterium long sword (Bifidobacterium longum), Bifidobacterium bifidum (Bifidobacterium bifidum) and Bifidobacterium brave (Bifidobacterium breve) may be any one or more selected from the group consisting of, preferably Lactobacillus plantarum (Lactobacillus plantarum) and / Or Bifidobacterium bifidum.

Lactobacillus is a generic term for microorganisms that generate energy by using sugars as a nutrient and produce lactic acid as a final product. The reason lactic acid bacteria are good for gut health is because the lactic acid bacteria produce acidic environment to prevent harmful bacteria such as welch bacteria from living.

When the activity of lactic acid bacteria in the intestine is active and a lot of lactic acid is generated, the intestinal environment is acidified, and as a result, the activity of bacteria harmful to humans is suppressed.

An abnormality in the body is a case where certain harmful microorganisms in the body are overpowered, causing the body to lose balance. There are harmful bacteria and lactic acid bacteria in our intestines. In order to keep the body healthy, the ratio of lactic acid bacteria is important. This is called'intestinal flora' and it means the balance of gut bacteria.

E. coli is one of many bacteria present in the human and animal intestines, especially in the large intestine. Escherichia coli usually does not show pathogenicity in the intestine, but when it enters areas other than the intestine, it causes cystitis, pyelonephritis, peritonitis, and sepsis.

Since E. coli is generated by manure and the like discharged from humans and animals, it is used as an indicator of pathogenic bacteria in terms of environment and health.

The prebiotic composition of the present invention is a mixture of nutritional sources of beneficial bacteria in the intestine, and when ingested, provides an optimal environment for metabolism of beneficial bacteria in the intestine, thereby enhancing the growth of beneficial bacteria in the intestine, and vice versa. By suppressing silver, the vitality effect can be easily exhibited.

The present invention provides a prebiotic composition comprising a sweet potato stem polymer fraction as an active ingredient.

The prebiotic composition may also be referred to as a composition for improving intestinal microflora or intestinal flora. This increases or decreases the efficacy of probiotics and improves intestinal health or improves intestinal function. Improving intestinal microflora or intestinal microflora may mean promoting the growth of beneficial bacteria in the intestine and suppressing the growth of harmful bacteria in the intestine, but maintaining the balance of beneficial and harmful bacteria in the intestine.

In the present invention, the'fraction' is a fraction obtained by additionally fractionating the sweet potato stem extract, that is, the sweet potato stem polymer fraction includes not only obtained using an extraction solvent, but also obtained by additionally applying a purification process thereto. . For example, various methods carried out additionally, such as fractionation obtained by passing through an ultrafiltration membrane having a constant molecular weight cut-off value, separation by various chromatography (made for separation according to size, charge, hydrophobicity or affinity), etc. Can be purified through.

The sweet potato stem polymer fraction is a fraction of a sweet potato stem extract having a molecular weight of 10 kDa or more, and may include 60 to 70% by weight of neutral sugar and other polysaccharides compared to the total polymer fraction. Further, the protein may further include 1 to 10% by weight.

The neutral polysaccharide may include, but is not limited to, rhamnose, fucose, arabinose, xylose, mannose, galactose, and glucose.

Sweet potato stem polymer fraction of the present invention comprises the steps of: (a) extracting the sweet potato stem with water, C1 to C4 alcohol or a mixed solvent thereof; And

(b) recovering a polymer fraction having a molecular weight of 10 kDa or higher using ultrafiltration or gel filtration chromatography from the extract; it may be prepared by a manufacturing method comprising a.

In the present invention, the sweet potato stem may be dried or dried, and crushed or powdered may be used.

The sweet potato stem is extracted with water, alcohol having 1 to 4 carbon atoms or a mixed solvent thereof, and the extraction method is not particularly limited. Preferably, the sweet potato stem extract may be a sweet potato stem hot water extract or an aqueous sweet potato ethanol solution, for example, a sweet potato stem alcohol extract.

In addition, the sweet potato stem extract may be a process of extracting the sweet potato stem, and repeatedly extracting it once or more with water, alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof.

After the step (a), a process of heating the sweet potato stem extract at 90 to 110° C. for 10 to 60 minutes may be further performed. The heating increases the elution of the soluble polysaccharide component, and denatures and precipitates the polymer protein contained as some impurities, thereby enhancing the polymer fraction acquisition and purity by centrifugation.

Sweet potato stem extract obtained through the step (a) is characterized in that the proportion of neutral polysaccharide is remarkably high. That is, the sweet potato stem extract extracted according to the above method contains a large amount of polysaccharide fraction.

In addition, after the step (a), the sweet potato stem extract may further include a step of removing solids from a known mixture such as centrifugation, filtration, which may be omitted if necessary, but preferably in centrifugation. I can do it.

Next, in the step (b), it may be to recover the fraction having a molecular weight of 10 kDa or more using ultrafiltration or gel filtration chromatography.

The method for obtaining the fraction is not particularly limited as long as it is a purification method based on molecular weight, but is preferably ultrafiltration or gel filtration chromatography, and more preferably gel filtration chromatography. In addition, the form of the final polysaccharide fraction may be an extract, concentrate, powder, or the like.

The step of purifying polysaccharides by removing low molecular substances and impurities by adding 50 to 100% of alcohol having 1 to 4 carbon atoms to the final polymer fraction may be further performed.

Specifically, the present invention obtained a sweet potato stem polymer fraction having a molecular weight of 10 kDa or more using the same method as the above, the sweet potato stem extract obtained by extraction with alcohol having 1 to 4 carbon atoms.

The term'fraction' in the present specification includes not only a fraction obtained by treating an extraction solvent, but also a processed product of a sweet potato stem polymer fraction. For example, the sweet potato stem polymer fraction may be prepared in powder form by additional processes such as distillation under reduced pressure and freeze drying or spray drying.

In addition, the sweet potato stem polymer fraction of the present invention contains 60-70% by weight of a neutral polysaccharide with a molecular weight of 10 kDa or more and the remaining amount of polysaccharides, and a saccharide (low molecular weight) having a molecular weight of 10 kDa or less may be excluded through a filtration process. have.

It has been known that prebiotic promotes proliferation of lactic acid bacteria mainly due to monosaccharides. However, the sweet potato stem polymer fraction of the present invention not only confirmed that it has a potency enhancing activity or activity against a large number of beneficial bacteria in the intestine, even though a low molecular weight (glycoside) having a molecular weight of 10 kDa or less was removed, as well as Lactococcus lactis For NCDO604(T) (Lactococcus lactis subsp. lactis), it was confirmed that at least three times higher activity was achieved than Fracto oligosaccharide (FOS) and sweet potato stem hydrothermal extract (FIG. 2).

Sweet potato stem polymer fraction of the present invention in the broad sense has a meaning including sweet potato stem polymer fraction, sweet potato stem polysaccharide fraction, sweet potato stem polymer processed, such as sweet potato stem polymer powder formulated to administer the sweet potato stem to animals. . Although the experiment was conducted with the sweet potato stem polymer fraction in the present invention, it will be predictable to those skilled in the art that the desired effect can be achieved in the same form as the sweet potato stem polymer processed product.

Meanwhile, in the present specification, the term'containing as an active ingredient' means to include an amount sufficient to achieve the efficacy or activity of the sweet potato stem polymer fraction. In one example, the sweet potato stem polymer fraction is used at a concentration of 10 to 1500 μg/ml, preferably 100 to 1000 μg/ml. Since the sweet potato stem polymer fraction is a natural product and does not have side effects to the human body even when it is excessively administered, the upper limit of the quantitative amount of the sweet potato stem polymer fraction contained in the composition of the present invention can be carried out by a person skilled in the art within an appropriate range.

Another aspect of the present invention relates to a composition for improving bowel health or improving bowel function, characterized in that it contains a sweet potato stem extract or a sweet potato stem polymer fraction as an active ingredient.

In the present invention, the sweet potato stem extract or the sweet potato stem polymer fraction has an effect of improving intestinal health or improving intestinal function by promoting intestinal health or improvement of intestinal beneficial bacteria having an inhibitory or preventive effect.

The intestinal health or intestinal function prevention and improvement effect of the present invention is known as having an intestinal health or intestinal function improvement effect in vitro test (Lactobacillus paracasei ATCC 25302(T) (Lactobacillus paracasei subsp. tolerans), lactose) Bacillus plantarum (Lactobacillus plantarum), Lactococcus lactis NCDO604(T) (Lactococcus lactis subsp. lactis), Bifidobacterium longum, Bifidobacterium bifidum and Bifidobacterium bifidum By confirming that the proliferation of E. coli (Escherichia coli) is enhanced, the possibility of improving intestinal health and intestinal function was confirmed. .

Particularly, when the sweet potato stem extract was used as an active ingredient, it was confirmed that the Lactobacillus plantarum and/or Bifidobacterium bifidum significantly improved compared to the conventional fructo-oligosaccharide, and the sweet potato stem polymer In the case of fractions, it was confirmed that Lactococcus lactis subsp. lactis (Lactococcus lactis subsp. lactis) has a significantly higher activity than conventional fructo-oligosaccharides.

That is, when the sweet potato stem extract or the sweet potato stem polymer fraction of the present invention is treated with various intestinal beneficial bacteria known to have an effect of improving intestinal health or improving intestinal function, the intestinal health or intestinal function is enhanced because the growth of the beneficial bacteria in the intestine is enhanced. It can be used for improvement, inhibition or prevention purposes.

The composition of the present invention is a pharmaceutical composition, and can be prepared using a pharmaceutically acceptable and physiologically acceptable adjuvant in addition to the sweet potato stem extract or the sweet potato stem polymer fraction as an active ingredient, and as an adjuvant, disintegrant, Sweetening agents, binders, coating agents, expanding agents, lubricants, lubricants or flavoring agents may be used.

The pharmaceutical composition may be preferably formulated into a pharmaceutical composition for administration, including one or more pharmaceutically acceptable carriers in addition to the active ingredients described above.

Formulations of the pharmaceutical composition can be granules, powders, tablets, coated tablets, capsules, suppositories, liquids, syrups, juices, suspensions, emulsions, drops or injectables. For example, for formulation in the form of tablets or capsules, the active ingredient can be combined with an oral, non-toxic pharmaceutically acceptable inert carrier such as ethanol, glycerol, water, and the like. In addition, if desired or necessary, suitable binders, lubricants, disintegrants and colorants can also be included in the mixture. Suitable binders include, but are not limited to, natural sugars such as starch, gelatin, glucose or beta-lactose, corn sweeteners, natural and synthetic gums such as acacia, trachocanth or sodium oleate, sodium stearate, magnesium stearate, sodium Benzoate, sodium acetate, sodium chloride, and the like. Disintegrants include, but are not limited to, starch, methyl cellulose, agar, bentonite, xanthan gum, and the like.

Acceptable pharmaceutical carriers in compositions formulated as liquid solutions include, as sterile and biocompatible, saline, sterile water, Ringer's solution, buffered saline, albumin injection solution, dextrose solution, maltodextrin solution, glycerol, ethanol and One or more of these components may be mixed and used, and other conventional additives such as antioxidants, buffers and bacteriostatic agents may be added as necessary. In addition, diluents, dispersants, surfactants, binders, and lubricants may be additionally added to formulate into injectable formulations such as aqueous solutions, suspensions, emulsions, pills, capsules, granules or tablets.

Furthermore, it can be preferably formulated according to each disease or ingredient using methods disclosed in Remington's Pharmaceutical Science, Mack Publishing Company, Easton PA by appropriate methods in the art.

The pharmaceutical composition of the present invention may be administered orally or parenterally, and in the case of parenteral administration, intravenous injection, subcutaneous injection, intramuscular injection, intraperitoneal injection, transdermal administration, etc., preferably oral administration. .

Suitable dosages of the pharmaceutical compositions of the invention vary by factors such as formulation method, mode of administration, patient's age, weight, sex, morbidity, food, time of administration, route of administration, rate of excretion, and response sensitivity, Usually, a skilled physician can easily determine and prescribe a dose effective for the desired treatment or prevention. According to a preferred embodiment of the present invention, the daily dosage of the pharmaceutical composition of the present invention is 0.001-10 g/kg.

The pharmaceutical composition of the present invention may be prepared in a unit dose form by formulating with a pharmaceutically acceptable carrier and/or excipient, or may be prepared by incorporating into a multi-dose container. At this time, the formulation may be in the form of a solution, suspension, or emulsion in an oil or aqueous medium, or may be in the form of ex, powder, granule, tablet, or capsule, and may further include a dispersant or stabilizer.

In another example, the sweet potato stem extract or the sweet potato stem polymer fraction as described above may be variously used as a food composition for improving, suppressing or preventing intestinal health or bowel function.

The food composition comprising the sweet potato stem extract or the sweet potato stem polymer fraction of the present invention as an active ingredient may be used in the form of various foods, for example, beverages, gum, tea, vitamin complexes, powders, granules, tablets, capsules, and the like.

The food composition according to the present invention may be formulated in the same manner as the pharmaceutical composition and used as a functional food or added to various foods. Foods to which the composition of the present invention can be added include, for example, beverages, alcoholic beverages, confectionery, diet bars, dairy products, meat, chocolate, pizza, instant noodles, other noodles, gums, ice cream, vitamin complexes, and health supplements And so on.

The food composition of the present invention may include, as an active ingredient, a sweet potato stem extract or a sweet potato stem polymer fraction, as well as ingredients commonly added in food production, for example, proteins, carbohydrates, fats, nutrients, seasonings, and Contains flavoring agents. Examples of the carbohydrates described above include monosaccharides, such as glucose, fructose, and the like; Disaccharides such as maltose, sucrose, oligosaccharides, etc.; And polysaccharides, for example, conventional sugars such as dextrin, cyclodextrin, and sugar alcohols such as xylitol, sorbitol, and erythritol. As flavoring agents, natural flavoring agents (tauumatin, stevia extract (eg, rebaudioside A, glycyrrhizine, etc.)) and synthetic flavoring agents (saccharin, aspartame, etc.) can be used. For example, when the food composition of the present invention is made of a drink and a beverage, in addition to the sweet potato stem extract or the sweet potato stem polymer fraction of the present invention, citric acid, liquid fructose, sugar, glucose, acetic acid, malic acid, juice, and various plant extracts are added. Can be included as

The present invention provides a health functional food comprising a food composition for improving, inhibiting or preventing bowel health or bowel function, including the sweet potato stem extract or the sweet potato stem polymer fraction as an active ingredient. Health functional food is a food prepared by adding sweet potato stem extract or sweet potato stem polymer fraction to food materials such as beverages, teas, spices, gums, confectionery, or by encapsulation, powdering, suspension, etc. It means that it has a certain effect, but it has the advantage of not having side effects that can occur when taking the drug for a long time using food as a raw material, unlike general drugs. The health functional food of the present invention obtained in this way is very useful because it can be taken on a daily basis. The added amount of the sweet potato stem extract or the sweet potato stem polymer fraction in the health functional food cannot be uniformly defined depending on the type of the health functional food, but may be added in a range that does not impair the original taste of the food. It is usually in the range of 0.01 to 50% by weight, preferably 0.1 to 20% by weight relative to the target food. In addition, in the case of a health functional food in the form of pills, granules, tablets or capsules, it is usually added in the range of 0.1 to 100% by weight, preferably 0.5 to 80% by weight. In one embodiment, the dietary supplement of the present invention may be in the form of pills, tablets, capsules or beverages.

In addition, the present invention provides the use of a sweet potato stem extract or a sweet potato stem polymer fraction for the manufacture of a medicine or food for improving, inhibiting or preventing intestinal health or bowel function. As described above, the sweet potato stem extract or the sweet potato stem polymer fraction may be used for the purpose of improving, preventing or treating intestinal health or bowel function.

In addition, the present invention provides a method for improving, preventing or treating intestinal health or bowel function, comprising administering an effective amount of a sweet potato stem extract or a sweet potato stem polymer fraction to a mammal.

The term "mammal" as used herein refers to a mammal that is the subject of treatment, observation or experiment, preferably human.

As used herein, the term "effective amount" refers to the amount of an active ingredient or pharmaceutical composition that induces a biological or medical response in a tissue system, animal or human, as considered by a researcher, veterinarian, physician or other clinician, as applicable Contains the amount that induces relief of symptoms. The effective amount and the number of administrations for the active ingredient of the present invention can be changed according to the desired effect. Therefore, the optimal dosage to be administered can be easily determined by those skilled in the art, and the level of intestinal health or intestinal function, the content of active ingredients and other ingredients in the composition, the type of formulation, and the patient's age, weight, general health status , Sex and diet, time of administration, route of administration and composition, secretion rate of the composition, duration of treatment, and drugs to be used simultaneously. In the prevention, treatment or improvement method of the present invention, in the case of an adult, when the sweet potato stem extract or the sweet potato stem polymer fraction is administered once to several times a day, it is preferable to administer it at a dose of 0.001 g/kg to 10 g/kg. Do.

In the treatment method of the present invention, the composition comprising the sweet potato stem extract or the sweet potato stem polymer fraction as an active ingredient is an oral, rectal, intravenous, intraarterial, intraperitoneal, intramuscular, intrasternal, transdermal, topical, intraocular or intradermal route. Can be administered in a conventional manner.

Hereinafter, a preferred embodiment is provided to help the understanding of the present invention, but the following examples are only illustrative of the present invention, and it is apparent to those skilled in the art that various changes and modifications are possible within the scope and technical scope of the present invention. It is no wonder that such variations and modifications fall within the scope of the appended claims.

Example 1. Sweet potato stem hot water extract

Domestic sweet potato stems grown in Gangwon-do were purchased and used. The sweet potato stem was dried in a drying oven at 50° C., and then pulverized to 1.0 mm or less using a grinder to prepare a sweet potato stem powder.

To 5 g of sweet potato stem powder, water corresponding to a 20-fold volume was added, and reflux extraction was performed at 80° C. for 3 hours to obtain a hot water extract. After filtering the hot water extract, it was concentrated under reduced pressure using a rotary vacuum evaporator, and lyophilized for 72 hours. Sweet potato stem hot water extract obtained by the above method was described as WSPE (Water-soluble sweet potato extract).

Example 2. Sweet potato stem polymer fraction

Domestic sweet potato stems grown in Gangwon-do were purchased and used. The sweet potato stem was dried in a drying oven at 50° C., and then pulverized to 1.0 mm or less using a grinder to prepare sweet potato stem powder.

To 100 g of sweet potato stem powder, distilled water corresponding to a 20-fold volume was added, and reflux extraction was performed at a temperature of 80° C. for 3 hours to obtain a hot water extract. After filtering the hot water extract, it is concentrated under reduced pressure to be 1/10 of the extract using a rotary vacuum evaporator, and 80% ethanol in 4 times the volume (v/v) of the concentrated hot water extract is added. It precipitated for 24 hours. After sedimentation, a precipitate was obtained by centrifugation (6000 rpm, 635 30g, 30 minutes), and a small amount of distilled water was added to re-dissolve the precipitate and dialysis membrane (MW cut off 12,000, Sigma-Aldrich Co., St. Louis, MO, USA) to remove the low-molecular substances by dialysis for 2-3 days and freeze-dried for 72 hours to obtain a sweet potato stem polymer fraction. The sweet potato stem polymer fraction obtained by the above method was named HSPE (High-molecular fraction from sweet potato extract).

<Test Example>

Test Example 1. Analysis per composition of the hot water extract of Example 1 and the polymer fraction of Example 2

Constituent sugar analysis is modified by partially modifying the method of Albersheim, etc., and then hydrolyzing each constituent sugar by derivatization with alditol acetate and aldonolactone, GC (Gas Chromatography ACME-6100, Young-Lin Co. Ltd. Anyang, Korea) ). The sample of Example 1 or Example 2 was hydrolyzed by reacting at 121° C. for 1.5 hr in 2 M TFA (trifluroacetic acid), dissolved in 1 ml of 1 M NH ₄ OH (ammonia solution), and 10 mg of NaBH ₄ It was reduced to 4 hr. After removing the residual NaBH ₄ by adding an appropriate amount of acetic acid, methanol was added and repeatedly dried to remove excess acetic acid and converted to alditol corresponding to each constituent.

To know the composition of the neutral sugar, 1 ml of acetic anhydride was added to each alditol and reacted at 121°C for 30 min to convert to alditol acetate, which was converted to chloroform/H ₂ O Separated and extracted with a two-phase solvent system, the extract was dried, dissolved in a small amount of acetone, and used as a sample for GC analysis.

Protein quantification was measured according to the Bradford method, and BSA (Bovine Serum Albumin, sigma aldrich) was used as a standard and diluted to a constant multiple of 1 mg/ml at the highest concentration. After reacting by adding 20 µl of the standard and the sample to 980 µl of the Bradford reagent, the absorbance at 595 nm was measured using a microplate reader. Protein quantification was calculated in terms of BSA.

The total sugar was measured according to the phenol-sulfuric acid method. After adding and stirring the same amount of 5% phenol solution in 0.5 ml of the sample solution, 2.5 ml of concentrated sulfuric acid (98%, v/v) was added and reacted at room temperature for 20 minutes to microplate. Absorbance was measured at 470 nm using a reader. For the determination of total sugar, a calibration curve was prepared using a glucose standard (sigma aldrich) and converted to the amount.

As a result, as a result of analyzing the physicochemical properties such as the content of main components of the hot water extract and the alcoholic extract from the sweet potato stem, the results are shown in Table 1 below.

division Example 1 (WSPE) Example 2 (HSPE) Extraction yield (%) 59.76 1.11 Chemical composition (%) Neutral sugar 52.13±0.16 63.26±1.85 Protein 1.67±0.11 5.01±0.48 Total dietary fiber (% by weight) 0.1 0.05 Inert dietary fiber (% by weight) 0.01 -

* The content ratio (%) of the chemical component is a ratio in a dry sample.

As shown in Table 1, the sweet potato stem hot water extract prepared from Example 1 showed a high yield of 59.76%, a protein content of 1.67% by weight, and a neutral sugar content of 52.13% by weight. On the other hand, the yield of the sweet potato stem polymer fraction prepared from Example 2 was as low as 1.11%, but the neutral sugar content was found to be 63.26% by weight. As a result, most of the sweet potato stem polymer fraction (HSPE) of Example 2 was analyzed to be occupied by sugar.

Among the sweet potato stems, it was confirmed that in the case of an inactive dietary fiber widely known to have an effect of improving intestinal health or intestinal function, it was hardly observed in the sweet potato stem hot water extract of Example 1 and the sweet potato stem polymer fraction of Example 2.

Test Example 2. Analysis of the proliferation activity of beneficial bacteria in the intestine of the hot water extract of Example 1 and the polymer fraction of Example 2

Isolation and identification of strains for measuring the activity of beneficial bacteria in the intestine

In order to analyze the prebiotic activity score of the sweet potato stem hot water extract of Example 1 and the sweet potato stem polymer fraction of Example 2, an intestinal beneficial bacteria was obtained. It selected those known as beneficial bacteria in the intestine. The bacteria were separated from fermented human intestines and traditional foods (miso, kimchi, etc.) or used for pre-sale at a biological resource center.

Lactobacillus paracasei subsp. tolerans, Lactobacillus plantarum, Lactococcus lactis subsp. lactis, Bifidobacterium long sword (Bifidobacterium longum), Bifidobacterium bifidum, Bifidobacterium breve and Escherichia coli were secured.

The Lactobacillus and Lactococcus strains were cultured using MRS medium, and the Bifidobacterium strains were cultured using BL medium, pH 6.5 ± 0.2, temperature 37° C., and still cultured for 48 hours. The strain was preserved through preservative anaerobic and freeze-dried storage or cell suspension freezing.

Isolation and identification of strains for measuring E. coli activity

For the analysis of the prebiotic activity score of the sweet potato stem hot water extract of Example 1 and the sweet potato stem polymer fraction of Example 2, E. coli was used by pre-sale at a biological resource center.

Cultivation of strains for the measurement of beneficial and E. coli activity in the intestine

For the culture of the isolated and identified strains, Lactobacilli MRS agar and Lactobacilli MRS broth were used as lactic acid bacteria, and BL agar and BL broth were used as Bifidobacterium strains. E. coli tryptic soy agar and tryptic soy broth were used. The medium was purchased from Difco.

The colonies of the strains were spread on agar medium corresponding to each strain, followed by primary incubation at 37°C for 24 hours, followed by inoculation in 10 ml of liquid medium and secondary incubation at 37°C for 24 hours. When the absorbance at 600 nm became 2.0, the culture medium was inoculated with 1% (v/v). For this culture, M9 minimal medium (MB cell) medium was used, and the M9 medium was prepared by adding glucose 2 g/L, CaCl ₂ 0.015 g/L, and MgSO ₄ 0.5 g/L.

Test Example 2-1. Prebiotic activity score analysis of sweet potato stem hot water extract

The obtained bacteria were added to 1% (v/v) of M9 medium each containing 10 mg/ml glucose to prepare a control.

In addition, 1% (v/v) of the obtained bacteria was added to M9 medium each containing 10 mg/ml fructooligosaccharides (FOS) to prepare a positive control.

The obtained bacteria were added to 1% (v/v) of M9 medium containing 10 mg/ml of sweet potato stem hot water extract, respectively, to prepare an experimental group.

Each group was inoculated with the bacteria and immediately (0 hours), 24 hours later, absorbance was measured at 600 nm using a microplate reader, and these values were substituted into Equation 1 below to show intestinal beneficial activity (prebiotic activity). score).

[Equation 1]

In the above formula,

*) probiotic log O.D on the prebiotic at 24 h is the absorbance measured at 600 nm using a microplate reader 24 hours after inoculation of Lactobacillus and Bifidobacterium bacteria in a positive control group or an experimental group.

*) probiotic log O.D. on the prebiotic at 0 h is the absorbance measured at 600 nm by inoculating Lactobacillus and Bifidobacterium bacteria in the positive control group or the experimental group and using a microplate reader.

*) probiotic log O.D. on glucose at 24h is the absorbance measured at 600 nm using a microplate reader 24 hours after inoculation of Lactobacillus and Bifidobacterium bacteria in the glucose control.

*) probiotic log O.D. on glucose at 0h is the absorbance measured at 600 nm using a microplate reader inoculated with Lactobacillus and Bifidobacterium bacteria in a glucose control.

*) Enteric log O.D on the prebiotic at 24 h is the absorbance measured at 600 nm using a microplate reader 24 hours after inoculating E. coli in the positive control group or the experimental group.

*) enteric log O.D. on the prebiotic at 0 h is the absorbance measured at 600 nm by inoculating E. coli in the positive control group or the experimental group and using a microplate reader.

*) enteric log O.D. on glucose at 24h is the absorbance measured at 600 nm using a microplate reader after 24 hours of inoculation with E. coli in the glucose control.

*) enteric log O.D. on glucose at 0h is the absorbance measured at 600 nm by inoculating E. coli in the glucose control and using a microplate reader.

1 is a graph showing the analysis of a prebiotic activity score in the intestine when a sweet potato stem hydrothermal extract of Example 1 is treated with various bacteria, FOS (fructo oligosaccharide). 1A is for Lactobacillus paracasei ATCC 25302(T) (Lactobacillus paracasei subsp. tolerans), FIG. 1B is Lactobacillus plantarum, FIG. 1C is Lactococcus lactis NCDO604(T) ( Lactococcus lactis subsp.lactis), Figure 1D is for Bifidobacterium longum, Figure 1E is for Bifidobacterium bifidum, Figure 1F is for Bifidobacterium breve .

Figure 3A is a graph showing the analysis of the activity (prebiotic activity score) for lactobacillus and lactococcus among the beneficial bacteria in the intestine for the total bacteria of the sweet potato stem hot water extract of Example 1, Figure 4A is the sweet potato stem hot water of Example 1 It shows the average of the intestinal beneficial activity (prebiotic activity score) for the total bacteria of the extract.

The conventional prebiotic fructo-oligosaccharide and the sweet potato stem hydrothermal extract of Example 1 were administered to each strain, and the prebiotic activity score was confirmed. Referring to Figure 1, the sweet potato stem hydrothermal extract of Example 1 according to the present invention was confirmed to exhibit the same or more activity compared to the conventional prebiotic fructo-oligosaccharides for the majority of intestinal beneficial bacteria, the activity against E. coli It was found to be inhibited. Most of the beneficial bacteria were found to have higher activity than the control group (up to 3-6 times).

Sweet potato stem hydrothermal extract of the present invention was confirmed to show the highest prebiotic activity score for Lactobacillus plantarum and Bifidobacterium bifidum. Specifically, it was confirmed to have a higher activity (up to 0.5-fold increase) than fructo-oligosaccharides, which are widely known as conventional prebiotics. The sweet potato stem hydrothermal extract of the present invention was confirmed to have activity similar to or higher than that of the positive control FOS, and was found to have high activity compared to the control group. Among them, the sweet potato stem hydrothermal extract of the present invention has higher prebiotic activity than the conventional prebiotic (fructo oligosaccharide) for Lactobacillus plantarum and Bifidobacterium bifidum. Was confirmed.

As shown in Figure 3A, as a result of comparing the activity on Lactobacillus and Lactococcus except the anaerobic strain Bifidobacterium, it was confirmed that the sweet potato stem hydrothermal extract of the present invention exhibits a similar level of activity to FOS. In addition, as a result of comparing the prebiotic activity for the overall gut beneficial bacteria, the sweet potato stem hydrothermal extract was 0.961, slightly lower than the FOS group (0.124), but it was confirmed that it increased by about 4 to 5 times compared to the case where nothing was treated. .

Test Example 2-2. Analysis of the prebiotic activity score in the intestine for sweet potato stem polymer fraction

The obtained bacteria were added to 1% (v/v) of M9 medium each containing 10 mg/ml glucose to prepare a control.

In addition, 1% (v/v) of the obtained bacteria was added to M9 medium each containing 10 mg/ml fructooligosaccharides (FOS) to prepare a positive control.

The obtained bacteria were added to 1% (v/v) of M9 medium containing 10 mg/ml of sweet potato stem polymer fraction (Example 2), respectively, to prepare an experimental group.

Each group was inoculated with the bacteria and immediately (0 hours), 24 hours later, absorbance was measured at 600 nm using a microplate reader, and these values were substituted into Equation 1 below to show intestinal beneficial activity (prebiotic activity). score).

[Equation 1]

In the above formula,

*) probiotic log O.D on the prebiotic at 24 h is the absorbance measured at 600 nm using a microplate reader 24 hours after inoculation of Lactobacillus and Bifidobacterium bacteria in a positive control group or an experimental group.

*) probiotic log O.D. on the prebiotic at 0 h is the absorbance measured at 600 nm by inoculating Lactobacillus and Bifidobacterium bacteria in the positive control group or the experimental group and using a microplate reader.

*) probiotic log O.D. on glucose at 24h is the absorbance measured at 600 nm using a microplate reader 24 hours after inoculation of Lactobacillus and Bifidobacterium bacteria in the glucose control.

*) probiotic log O.D. on glucose at 0h is the absorbance measured at 600 nm using a microplate reader inoculated with Lactobacillus and Bifidobacterium bacteria in a glucose control.

*) Enteric log O.D on the prebiotic at 24 h is the absorbance measured at 600 nm using a microplate reader 24 hours after inoculating E. coli in the positive control group or the experimental group.

*) enteric log O.D. on the prebiotic at 0 h is the absorbance measured at 600 nm by inoculating E. coli in the positive control group or the experimental group and using a microplate reader.

*) enteric log O.D. on glucose at 24h is the absorbance measured at 600 nm using a microplate reader after 24 hours of inoculation with E. coli in the glucose control.

*) enteric log O.D. on glucose at 0h is the absorbance measured at 600 nm by inoculating E. coli in the glucose control and using a microplate reader.

2 is a graph showing the analysis of the prebiotic activity score in the intestine when the sweet potato stem polymer fraction of Example 2, FOS (fructo oligosaccharide) is treated with various bacteria. 2A is for Lactobacillus paracasei ATCC 25302(T) (Lactobacillus paracasei subsp. tolerans), FIG. 2B is Lactobacillus plantarum, FIG. 2C is Lactococcus lactis NCDO604(T) ( For Lactococcus lactis subsp.lactis, Figure 2D is Bifidobacterium longum, Figure 2E is Bifidobacterium bifidum, Figure 2F is Bifidobacterium breve It is about.

Figure 3B shows the average of the activity (prebiotic activity score) for lactobacillus and lactococcus among intestinal beneficial bacteria in the sweet potato stem polymer fraction, Figure 4B shows the beneficial bacteria activity in the intestinal flora against the total bacteria of the sweet potato stem polymer fraction of Example 2 (prebiotic activity score).

The conventional prebiotic fructo-oligosaccharide and the sweet potato stem polymer fraction of Example 2 were administered to each strain, and the prebiotic activity score was confirmed. 2, it was confirmed that the majority of the sweet potato stem polymer fractions of Example 2 according to the present invention show the same or higher activity than the conventional prebiotic fructo-oligosaccharides for most intestinal beneficial bacteria. It was confirmed that the activity was high, and it was confirmed that the activity was inhibited against E. coli (up to 3-6 times).

The sweet potato stem polymer fraction of the present invention seemed to maintain a similar level of activity against the majority of beneficial bacteria when compared to the sweet potato stem hydrothermal extract treatment, however, Lactococcus lactis subsp. lactis).

As shown in FIG. 3B, as a result of comparing the activity on Lactobacillus and Lactococcus except the anaerobic strain Bifidobacterium, the sweet potato stem polymer fraction of the present invention has a prebiotic activity score of 1.2 times higher than FOS. Confirmed.

Furthermore, as a result of comparing the prebiotic activity for the overall gut beneficial bacteria, the sweet potato stem polymer fraction was 1.083, slightly lower (0.352) than the FOS group, but significantly increased (0.7-0.8 times higher) compared to the case where nothing was treated. Confirmed.

As a result of comparing the prebiotic activity for the total beneficial bacteria, it was confirmed that both the sweet potato stem hot water extract and the polymer fraction have similar efficacy to the positive control FOS.

Summarizing the above results, it can be confirmed that in the case of the sweet potato stem polymer fraction, the ability to increase prebiotic activity against beneficial bacteria such as the facultative anaerobic strains Lactobacillus and Lactococcus is significantly superior to that of FOS by 1.2 times or more. In addition, since the sweet potato stem hot water extract contains portions other than the polymer fraction, it can be seen that it exhibits FOS and a flat level of prebiotic activity with respect to all beneficial bacteria such as Lactobacillus, Lactococcus, and Bifidobacteria, anaerobic bacteria. . However, it was confirmed that it is significantly superior to normal.

When a component other than the polymer fraction is mixed, it is believed that the multiplication effect between them causes the proliferation and enhancement effect to beneficial bacteria such as the facultative anaerobic strains Lactobacillus and Lactococcus, and is desired or the desired effect. Depending on the hot water extract and the polymer fraction can be appropriately selected and used.

Hereinafter, a formulation example of the composition containing the powder of the present invention will be described, but the present invention is not intended to limit it, but only to be specifically described.

Formulation Example 1. Preparation of powder

Sweet potato stem hydrothermal extract obtained in Example 1; or

Sweet potato stem polymer fraction obtained in Example 2; 500 mg

Lactose 100 mg

Talc 10 mg

The above ingredients are mixed and filled in an airtight fabric to prepare a powder.

Formulation Example 2. Preparation of tablets

Sweet potato stem hydrothermal extract obtained in Example 1; or

Sweet potato stem polymer fraction obtained in Example 2; 300 mg

Corn starch 100 mg

Lactose 100 mg

Magnesium stearate 2 mg

After mixing the above ingredients, tablets are prepared by tableting according to a conventional tablet manufacturing method.

Formulation Example 3. Preparation of capsules

Sweet potato stem hydrothermal extract obtained in Example 1; or

Sweet potato stem polymer fraction obtained in Example 2; 200 mg

Crystalline cellulose 3 mg

Lactose 14.8 mg

Magnesium stearate 0.2 mg

According to a conventional capsule preparation method, the above ingredients are mixed and filled into a gelatin capsule to prepare a capsule.

Formulation Example 4. Preparation of injection

Sweet potato stem hydrothermal extract obtained in Example 1; or

Sweet potato stem polymer fraction obtained in Example 2; 600 mg

Mannitol 180 mg

Injectable sterile distilled water 2974 mg

Na ₂ HPO _4, 12H ₂ O 26 mg

It is prepared with the above-mentioned ingredient content per ampoule according to the conventional injection method.

Formulation Example 5. Preparation of liquid formulation

Sweet potato stem hydrothermal extract obtained in Example 1; or

Sweet potato stem polymer fraction obtained in Example 2; 4 g

Isomerized sugar 10 g

5 g of mannitol

Purified water

Each component is added to the purified water according to the conventional method for dissolving it, dissolved in a suitable amount of lemon flavor, mixed with the above components, added to purified water, adjusted to 100 g, and then sterilized by filling in a brown bottle. .

Formulation Example 6. Preparation of granules

Sweet potato stem hydrothermal extract obtained in Example 1; or

Sweet potato stem polymer fraction obtained in Example 2; 1,000 mg

Vitamin mixture

Vitamin A Acetate 70 μg

Vitamin E 1.0 mg

Vitamin B1 0.13 mg

Vitamin B2 0.15 mg

Vitamin B6 0.5 mg

Vitamin B12 0.2 μg

Vitamin C 10 mg

Biotin 10 μg

Nicotinic acid amide 1.7 mg

50 ㎍ folic acid

Calcium Pantothenate 0.5 mg

Suitable amount of mineral mixture

Ferrous sulfate 1.75 mg

Zinc oxide 0.82 mg

Magnesium carbonate 25.3 mg

Potassium phosphate 15 mg

Dibasic calcium phosphate 55 mg

Potassium citrate 90 mg

Calcium carbonate 100 mg

Magnesium chloride 24.8 mg

Although the composition ratio of the vitamin and mineral mixture is a composition suitable for relatively granule preparation in a preferred embodiment, the mixing ratio may be arbitrarily modified, and the above ingredients are mixed according to a conventional granule preparation method, and then granulated It can be prepared and used in the production of health functional food composition according to a conventional method.

Formulation Example 7. Preparation of functional beverage

Sweet potato stem hydrothermal extract obtained in Example 1; or

Sweet potato stem polymer fraction obtained in Example 2; 1,000 mg

1,000 mg citric acid

Oligosaccharide 100 g

2 g plum juice

Taurine 1 g

900 mL total by adding purified water

After mixing the above components according to a conventional health drink manufacturing method, and stirring and heating at 85° C. for about 1 hour, the resulting solution is filtered, obtained in a sterilized 2 L container, sealed and sterilized, and then refrigerated and stored It is used for preparing the functional beverage composition of the present invention.

Although the above composition ratio is a mixture of components suitable for a preference drink in a preferred embodiment, the composition ratio may be arbitrarily modified according to regional and ethnic preferences such as demand hierarchy, demand country, and usage.

Claims (7)

delete A prebiotic composition comprising a sweet potato stem polymer fraction obtained by fractionating a sweet potato stem extract with a molecular weight of 10 kDa or more, as an active ingredient,
The sweet potato stem extract is extracted with water, ethanol or a mixed solvent thereof,
The prebiotic composition has a prebiotic activity that proliferates beneficial bacteria in the intestine relative to harmful bacteria in the intestine,
The intestinal beneficial bacteria are any one or more selected from the group consisting of Lactobacillus paracasei, Lactobacillus plantarum, Lactococcus lactis, Bifidobacterium longum, Bifidobacterium bifiderm and Bifidobacterium brave ,
The intestinal harmful bacteria is a prebiotic composition, characterized in that E. coli. delete delete As a food composition for improving intestinal health or intestinal function, containing a sweet potato stem polymer fraction obtained by fractionating a sweet potato stem extract with a molecular weight of 10 kDa or more as an active ingredient,
The sweet potato stem extract is extracted with water, ethanol or a mixed solvent thereof,
The composition has a prebiotic activity that proliferates beneficial bacteria in the intestine relative to harmful bacteria in the intestine,
The intestinal beneficial bacteria are any one or more selected from the group consisting of Lactobacillus paracasei, Lactobacillus plantarum, Lactococcus lactis, Bifidobacterium longum, Bifidobacterium bifiderm and Bifidobacterium brave ,
The intestinal harmful bacteria is E. coli food composition for improving intestinal health or bowel function. delete delete

Images