KR20130052910A - Silk peptide for improving hypercholesterolemia and hyperlipidemia - Google Patents
Silk peptide for improving hypercholesterolemia and hyperlipidemia Download PDFInfo
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- A23V2200/3262—Foods, ingredients or supplements having a functional effect on health having an effect on blood cholesterol
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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Abstract
본 발명은 혈중 콜레스테롤 및 혈중 중성지방을 낮추는 개선 효과가 있는 고분자 실크 펩타이드의 구성물에 관한 것으로, 실크(견사)를 산 가수분해 방법 또는 염화칼슘을 이용하여 가수분해하여 동결 건조한 후, 분말상으로 제조한 고분자 실크 펩타이드 조성물로써, 본 발명의 실크 펩타이드의 분자량은 10,000~50,000 으로 체내에서 흡수되지 않아 혈중 콜레스테롤 및 혈중 중성지방을 낮추는 기능성 식품 또는 의약품으로 개발하거나 각 성분에 대한 활성물질의 개발에 기여할 수 있다. The present invention relates to a composition of a polymer silk peptide having an improvement effect of lowering blood cholesterol and triglyceride in blood, wherein the silk (silk silk) is hydrolyzed using an acid hydrolysis method or calcium chloride, and freeze-dried to prepare a polymer in powder form. As a silk peptide composition, the molecular weight of the silk peptide of the present invention is 10,000 to 50,000, which is not absorbed by the body, so that it can be developed as a functional food or medicine that lowers blood cholesterol and blood triglycerides or contributes to the development of active substances for each component.
Description
본 발명은 고콜레스테롤증 및 고지혈증 개선의 복합 기능을 가지는 실크 펩타이드 조성물에 관한 것으로서, 보다 상세하게는 실크 단백질을 가수 분해하여 10,000~50,000의 중량 평균 분자량을 갖으며, 고콜레스테롤증 및 고지혈증 개선의 복합 기능을 발휘하는 실크 펩타이드 조성물에 관한 것이다.
The present invention relates to a silk peptide composition having a complex function of hypercholesterolemia and hyperlipidemia, and more particularly, to a silk protein hydrolyzed to have a weight average molecular weight of 10,000 to 50,000, and a combination of hypercholesterolemia and hyperlipidemia improvement. A silk peptide composition that functions.
실크는 피브로인과 세리신으로 구성되어 있는 단백질계 섬유로 예전에는 옷감을 만드는 데에만 사용되었으나, 최근에는 연구에 의하여 기능성 식품 및 화장품으로 제조되어 사용되고 있다. 이러한 실크 단백질을 기능성 식품 또는 약제의 유효 성분으로 이용하기 위하여, 실크 단백질로부터 저분자 실크 펩타이드를 얻기 위한 다양한 방법이 제시되어 있다. 예컨대, 대한민국 특허등록 제 10-0443785 호에는 염산을 이용하여 실크 아미노산의 제조방법이 개시되어 있고, 대한민국 특허등록 제 10-0420824 호에는 알카리를 이용하여 실크 펩타이드를 제조하는 방법이 개시되어 있으며, 대한민국 특허등록 제 10-0881210 호에는 효소를 이용하여 실크 펩타이드를 제조방법이 개시되어 있다.Silk is a protein-based fiber composed of fibroin and sericin, which was previously used only for making fabrics, but recently has been manufactured and used as a functional food and cosmetics by research. In order to use such silk proteins as active ingredients of functional foods or pharmaceuticals, various methods for obtaining low molecular weight silk peptides from silk proteins have been proposed. For example, Korean Patent Registration No. 10-0443785 discloses a method for producing silk amino acids using hydrochloric acid, and Korean Patent Registration No. 10-0420824 discloses a method for preparing silk peptides using alkali, and Korea Patent Registration No. 10-0881210 discloses a method for producing silk peptides using enzymes.
또한 이렇게 제조된 분자량 100~2,000 정도의 저분자 실크 펩타이드에 대하여 당뇨 개선, 인지기능 개선 등의 효능이 있는 것이 여러가지 제시되어 있다. 예컨대, 대한민국 특허등록 제 10-0947547 호에는 당뇨의 치료 및 예방효과를 가지는 실크 펩타이드에 대한 내용이 개시되어 있고, 대한민국 특허등록 제 10-0494358 호에는 두뇌 인지 기능 개선에 효과가 있는 실크 펩타이드에 대한 내용이 개시되어 있다. In addition, the low molecular weight silk peptides having a molecular weight of about 100 to 2,000 thus produced have been shown to have various effects such as diabetes improvement and cognitive function. For example, Korean Patent Registration No. 10-0947547 discloses a silk peptide having a therapeutic and prophylactic effect of diabetes, and Korean Patent Registration No. 10-0494358 discloses a silk peptide effective for improving brain cognitive function. The contents are disclosed.
종래의 실크 펩타이드 제조방법들은 염산 또는 효소를 이용하여 섬유형태의 실크 견사를 가수분해하여 분자량이 작은 저분자 펩타이드나 아미노산 수준으로 아주 작게 분해하는 방법을 개발하는데 초점을 맞추어져 있었다. 따라서 최종 제품의 분자량은 대부분 100 ~ 2,000 정도의 아미노산이나 저분자 펩타이드 정도 크기의 수준이며, 가수분해된 생성물 중에 커다란 것의 분자량도 10,000 내외의 것들로 이루어져 있다. 견사 섬유는 그 자체로는 소화 흡수될 수 없기 때문에 그동안 여러가지 방법을 이용하여 저분자화하여 어떻게 하면 소화 흡수율을 높일 수 있을까에 초점을 맞춘 연구가 이루어져 왔으며, 개발된 가장 대표적인 방법이 산과 효소를 이용하여 가수분해하는 방법이다. 산과 효소에 의해 저분자화된 아미노산이나 저분자 펩타이드는 90% 이상 인체에 흡수될 수 있었으며, 이렇게 제조된 아미노산이나 저분자 펩타이드들에서 당뇨 개선, 인지기능 개선등의 효능이 발견되었고, 저분자 펩타이드 물질 중에서 어떤 것들이 이러한 효능을 나타내는지에 대한 추가적인 연구들이 이루어지고 있다. Conventional silk peptide production methods have been focused on developing a method of hydrolyzing a silk silk in the form of fiber using hydrochloric acid or an enzyme to decompose to a very low molecular weight peptide or amino acid level. Therefore, the molecular weight of the final product is mostly about 100 to 2,000 amino acids or about the size of the small molecule peptide, and the molecular weight of the large hydrolyzed products is composed of about 10,000. Since silk fiber cannot be digested and absorbed by itself, research has focused on how to lower the molecular weight by using various methods to increase digestive absorption rate, and the most representative method developed by using acid and enzyme It is a method of hydrolysis. Amino acids and low molecular weight peptides made by acids and enzymes could be absorbed by the human body more than 90%. The amino acids and low molecular weight peptides were found to be effective in improving diabetes and cognitive function. Further studies are underway to show this efficacy.
그러나 10,000 이상의 큰 분자량의 고분자 실크 펩타이드에 대해서는, 이들 물질이 인체에서 흡수가 되지 않는다는 이유 때문에 이에 대한 제조나 연구가 전혀 이루어지고 있지 않은 실정이다.
However, for the high molecular weight high molecular silk peptide of 10,000 or more, there is no manufacturing or research on this because of the fact that these substances are not absorbed in the human body.
본 발명자들은 고콜레스테롤증 및 고지혈증을 개선할 수 있는 복합기능을 가지는 실크 펩타이드 조성물을 개발하고자 예의 연구 노력한 결과, 가수 분해를 통하여 수득한 10,000 이상의 중량 평균 분자량을 갖는 고분자 실크 펩타이드가 우수한 복합기능 활성을 갖는다는 것을 확인함으로써 본 발명을 완성하게 되었다.The present inventors earnestly researched to develop a silk peptide composition having a complex function that can improve hypercholesterolemia and hyperlipidemia, and as a result, the polymer silk peptide having a weight average molecular weight of 10,000 or more obtained through hydrolysis has excellent complex functional activity. By confirming that the present invention has been completed, the present invention was completed.
따라서, 본 발명의 목적은 고콜레스테롤증 및 고지혈증을 개선하는 복합기능 활성을 갖는 실크 펩타이드 조성물을 제공하는 데 있다.
Accordingly, it is an object of the present invention to provide a silk peptide composition having a multifunctional activity that improves hypercholesterolemia and hyperlipidemia.
본 발명의 양태에 따르면, 본 발명은 실크 단백질을 가수 분해하여 중량 평균 분자량이 10,000-100,000이고 고콜레스테롤증 및 고지혈증 개선의 복합활성을 가지는 실크 펩타이드 조성물을 제공한다.
According to an aspect of the present invention, the present invention provides a silk peptide composition hydrolyzing silk protein having a weight average molecular weight of 10,000-100,000 and having a combined activity of hypercholesterolemia and hyperlipidemia improvement.
본 발명의 방법에서 출발물질로 누에고치가 이용된다. 본 명세서에서 용어 "실크"는 견사 곤충이 토사하여 만든 섬유를 의미하며, 바람직하게는 누에 (Bombyx mori)가 토사하는 가잠사를 의미한다.A cocoon is used as starting material in the process of the invention. As used herein, the term "silk" means a fiber made by silkworm insects, and preferably means a silkworm sanded by a silkworm (Bombyx mori).
누에고치를 이루는 실크 단백질은 피브로인 및 세리신으로 이루어져 있고, 피브로인과 세리신은 각각 평균 75%와 25%의 비율로 존재한다. 피브로인 단백질과 세리신은 전혀 다른 아미노산 조성을 보이는 서로 다른 단백질이다. 최근의 연구 결과에 의하면 실크 피브로인은 H-사슬 (350 kDa) 및 L-사슬 (26 kDa)이 S-S 결합을 하고, 당단백질인 P25 (30 kDa)가 상기 두 사슬과 비공유 결합으로 연결된 구조를 하고 있으며, 각각 6:6:1의 몰 구성을 하고 있는 거대 단백질로 규명되었으며, 이러한 구조로 인해 결정영역과 비결정영역이 연속 교환 배열된 블록 형태의 고분자 성질을 가지게 된다.Silk cocoon silk protein consists of fibroin and sericin, fibroin and sericin are present on average 75% and 25%, respectively. Fibroin protein and sericin are different proteins with completely different amino acid compositions. According to recent research results, silk fibroin has a structure in which H-chain (350 kDa) and L-chain (26 kDa) are SS-bonded, and glycoprotein P25 (30 kDa) is non-covalently linked to the two chains. It was identified as a macromolecular protein having a mole composition of 6: 6: 1, and this structure has a polymer property of a block form in which crystalline and amorphous regions are continuously exchanged.
본 발명의 바람직한 구현예에 따르면, 이용되는 실크 단백질은 실크 피브로인이다.According to a preferred embodiment of the invention, the silk protein used is silk fibroin.
본 발명의 바람직한 구현예에 따르면, 실크 피브로인의 가수 분해는 (a) 정련, (b) 무기염 용액에서의 분해, (c) 무기염의 제거의 방법을 통해 실시될 수 있다. 상기한 방법에 의해 분해되어 생성된 실크 펩타이드의 중량 평균 분자량은 약 10,000-100,000의 범위를 나타낼 수 있으며, 당뇨, 고콜레스테롤증 및 고지혈증 개선의 복합 기능성을 가진다.According to a preferred embodiment of the present invention, the hydrolysis of silk fibroin can be carried out through the method of (a) refining, (b) decomposition in inorganic salt solution, (c) removal of inorganic salt. The weight average molecular weight of the silk peptides produced by decomposition by the above method may range from about 10,000-100,000, and has a complex function of improving diabetes, hypercholesterolemia and hyperlipidemia.
상기 정련은 누에고치를 열수에서 가열하여 실시되는데, 바람직하게는 80~130℃, 보다 바람직하게는 120℃에서 가열하여 세리신을 제거한다. 또한 열수에 탄산나트륨이나 계면활성제를 첨가하여 실시할 수도 있다.The refining is carried out by heating the cocoon in hot water, preferably 80 to 130 ℃, more preferably at 120 ℃ to remove sericin. Moreover, it can also carry out by adding sodium carbonate and surfactant to hot water.
상기 무기염 용액에서의 분해는 통상적으로 염산염을 이용하는 데, 바람직하게는 염화칼슘, 염화마그네슘, 염화아연, 보다 바람직하게는 염화칼슘을 사용한다. 또한 여기에 에탄올을 추가로 혼합하여 사용할 수 있다. The decomposition in the inorganic salt solution is usually used hydrochloride, preferably calcium chloride, magnesium chloride, zinc chloride, more preferably calcium chloride. In addition, ethanol may be further mixed and used therein.
상기 단계 (b)는 실크 피브로인을 염화칼슘, 물 및 에탄올이 혼합된 용액에서 60-95℃에서 반응시켜 용해하여 실시하는 것이 바람직하며, 보다 바람직하게는 70-95℃, 가장 바람직하게는 75-85℃에서 용해한다. 이와 같은 칼슘염 용액에서의 분해에 의해 실크 피브로인은 분자량 10,000-50,000 정도의 비교적 고분자 펩타이드로 분해된다. Step (b) is preferably performed by dissolving silk fibroin in a solution containing calcium chloride, water and ethanol at 60-95 ° C, more preferably 70-95 ° C, most preferably 75-85 Dissolve at ° C. By the decomposition in the calcium salt solution, silk fibroin is decomposed into a relatively high molecular peptide of about 10,000-50,000 molecular weight.
상기 단계 (c)에서 염을 제거하는 방법은 당업계에 공지된 다양한 방법을 통하여 실시할 수 있으며, 예컨대, 투석, 한외여과, 겔 여과 크로마토그래피 또는 전기 탈염 방법 등이 있으며, 특정한 중량 분자량 범위만을 선택적으로 분리하기 위하여 이 방법들을 조합하여 사용할 수 있다. The method of removing the salt in step (c) may be carried out through various methods known in the art, for example, dialysis, ultrafiltration, gel filtration chromatography or electrodesalting, etc., and only a specific weight molecular weight range. These methods can be used in combination for selective separation.
본 발명의 방법에 의해 제조된 실크 펩타이드는 높은 분자량을 갖기 때문에 체내에서 흡수가 잘 되지 않는다. 실크 피브로인의 독특한 다공성 구조는 높은 흡착 성질을 가지기 때문에 실크 피브로인과 함께 섭취된 지방 성분을 강하게 흡착할 수 있다. 그 결과 실크 피브로인에 흡착된 지방 성분은 체내에 흡수되지 못하고 배설되어 지방의 흡수를 저해하게 되며, 그 결과 혈중 지방 농도 저하, 혈중 콜레스테롤 농도 저하의 개선 효과를 가져올 수 있다.
Silk peptides prepared by the method of the present invention have a high molecular weight and are not well absorbed by the body. Silk fibroin's unique porous structure has high adsorption properties, so it can strongly adsorb fat components ingested with silk fibroin. As a result, the fat component adsorbed on silk fibroin is not excreted in the body and is excreted to inhibit the absorption of fat. As a result, it is possible to reduce blood fat concentration and lower blood cholesterol concentration.
본 발명은 고콜레스테롤증 및 고지혈증 개선의 복합 활성을 가지는 실크 펩타이드 조성물을 제공한다. 본 발명은 실크 단백질로부터 고콜레스테롤증 및 고지혈증 개선의 복합 활성을 가지는, 거의 체내에 흡수되지 않는 높은 분자량의 실크 펩타이드 조성물을 제공한다.
The present invention provides a silk peptide composition having a combined activity of hypercholesterolemia and hyperlipidemia improvement. The present invention provides a high molecular weight silk peptide composition that is almost not absorbed into the body, having a combined activity of improving hypercholesterolemia and hyperlipidemia from silk proteins.
하기의 실시예에서 예증된 바와 같이, 본 발명의 방법에 의해 제조된 실크 펩타이드는 고콜레스테롤증 및 고지혈증 의 치료 및 예방에 이용될 수 있다.
As illustrated in the examples below, silk peptides prepared by the methods of the present invention can be used for the treatment and prevention of hypercholesterolemia and hyperlipidemia.
이하, 실시예를 통하여 본 발명을 더욱 상세히 설명하고자 한다. 이들 실시예는 오로지 본 발명을 보다 구체적으로 설명하기 위한 것으로서, 본 발명의 요지에 따라 본 발명의 범위가 이들 실시예에 의해 한정되는 것은 아니다.
Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are only for illustrating the present invention more specifically, and the scope of the present invention is not limited by these examples according to the gist of the present invention.
실시예 1. 실크 피브로인의 정련Example 1 Refining Silk Fibroin
본 발명에 사용한 실크 단백질은 가잠 (Bombyx mori) 를 사육하여 얻은 누에고치에서 번데기를 제거하고 정련하여 사용하였다. 번데기를 제거한 누에고치에 50배의 물을 넣고 섭씨120도에서 20분간 가열하고, 깨끗한 물로 수세하고 건조시켜, 세리신 단백질이 제거된 순수한 실크 피브로인 단백질을 얻었다. The silk protein used in the present invention was used after removing pupa from the cocoon obtained by breeding the gambo (Bombyx mori). 50 times of water was added to the cocoon from which the pupa was removed, heated at 120 degrees Celsius for 20 minutes, washed with clean water and dried to obtain pure silk fibroin protein from which sericin protein was removed.
실시예 2. 고분자 실크 펩타이드의 제조방법Example 2. Preparation of Polymer Silk Peptides
실시예 1 에서 얻은 정련된 실크 피브로인 단백질을 5M 염화칼슘 용액에 넣고, 섭씨 90도로 가열하면서 에탄올을 가하여 실크 피브로인 단백질을 완전히 녹여 가수분해하였다. 거즈등을 여러겹 겹쳐서 번데기 조각등 커다란 이물질을 제거하고, 여과지 (Whatman No.1) 를 사용하여 불용성 물질을 제거하였다. 염화칼슘의 제거는 a) 물에 투석하는 방법, b) 전기탈염기를 사용하는 방법, c) 한외여과를 이용하는 방법, d) 겔크로마토그래피를 이용하는 방법을 이용하였다. 모든 방법에서 염화칼슘은 잘 제거되었으며, 이 후 작업에서 동일한 결과를 얻을 수 있었다. 다만, 전기탈염기 방법은 빠른 시간에 대량의 시료를 처리할 수 있는 장점이 있는 반면, 실크 단백질의 손실이 많은 단점이 있었다. 나머지 방법들은 대량의 시료를 처리하기 어려운 단점과 시간이 많이 걸리는 단점이 있는 반면, 실크 단백질의 손실이 적은 장점을 가지고 있었다. 염화칼슘이 제거된 고분자 실크 피브로인 펩타이드 용액을 동결 건조하여 분말로 제조하였다.
The refined silk fibroin protein obtained in Example 1 was placed in a 5M calcium chloride solution, and ethanol was added while heating at 90 degrees Celsius to completely dissolve and hydrolyze the silk fibroin protein. Multiple layers of gauze were stacked to remove large foreign objects such as pupa pieces, and filter paper (Whatman No. 1) was used to remove insoluble matters. Calcium chloride was removed by using a) dialysis in water, b) using an electrodesulfate, c) using ultrafiltration, and d) using gel chromatography. Calcium chloride was well removed in all methods and the same result was obtained in subsequent work. However, the electrodesalter method has the advantage of being able to process a large amount of samples in a fast time, while there are many disadvantages of silk protein loss. The remaining methods had the disadvantages of being difficult and time-consuming to process a large amount of samples, while having the advantage of low loss of silk protein. The calcium chloride-free polymeric silk fibroin peptide solution was freeze-dried to prepare a powder.
실시예 3. 고분자 실크 펩타이드의 분자량 측정Example 3. Determination of Molecular Weight of Polymer Silk Peptides
상기 실시예 2에서 제조된 고분자 실크 펩타이드에 대하여 겔 투과 크로마토그래피법에 의하여 분자량을 계산하였다. 0.2M sodium phosphate 완충용액에 고분자 실크 펩타이드를 녹이고, 크로마토그래피를 수행하여 280nm 흡광도 값으로부터 분자량의 분포를 계산하였으며, 실험 결과, 고분자 실크 펩타이드의 중량 평균 분자량은 26 kDa 이었다.
The molecular weight of the polymer silk peptide prepared in Example 2 was calculated by gel permeation chromatography. The polymer silk peptide was dissolved in 0.2 M sodium phosphate buffer and chromatographed to calculate the molecular weight distribution from the absorbance value of 280 nm. As a result, the weight average molecular weight of the polymer silk peptide was 26 kDa.
실시예 4. 고분자 실크 펩타이드의 콜레스테롤 및 중성지방 개선 효과Example 4 Cholesterol and Triglyceride Improvement Effects of Polymer Silk Peptides
임상실험 연구자는 비만인 남녀 20명 (남자 8명, 여자 12명, 평균 연령 48.8±6.5 세) 을 대조군 5명 과 실험군 15명으로 나누고 임상실험을 실시하였다. 고분자 실크 펩타이드 500mg 을 1일 3회, 식사하기 직전에 섭취하였으며 2개월동안 진행하였다. 대조군은 실크 펩타이드 대신 위약을 섭취하였다. 임상실험을 시작하기 전에 각 항목에 대한 측정을 하였으며, 이 후 1개월 단위로 각 항목을 측정하였다. 실험 결과는 표 1과 같다.
The investigator divided 20 obese men and women (8 males, 12 females, mean age 48.8 ± 6.5 years) into 5 control groups and 15 experimental groups. 500 mg of polymer silk peptide was taken 3 times a day, just before meals, and proceeded for 2 months. The control group received placebo instead of silk peptide. Before starting the clinical trial, each item was measured and then each item was measured on a monthly basis. The experimental results are shown in Table 1.
총콜레스테롤 (%)HDL Cholesterol /
Total Cholesterol (%)
총콜레스테롤 (%)HDL Cholesterol /
Total Cholesterol (%)
임상실험을 시작할 당시 총콜레스테롤, LDL 콜레스테롤, 중성지방이 모두 정상 범위를 초과하는 수치였으나, 고분자 실크 펩타이드를 섭취하기 시작한 후, 2개월동안 대조군의 경우 변화가 없었던 반면, 실험군에서는 이들 수치가 점차 감소하여 총콜레스테롤, LDL 콜레스테롤, 중성지방 모두 정상치 근처까지 개선되는 결과를 얻을 수 있었다. HDL 콜레스테롤은 처음부터 정상범위인 상태였으며, 2개월 이후에도 정상범위를 유지하고 있었고, 총콜레스테롤에 대한 HDL 콜레스테롤의 비율은 23.5% 에서 25.1%로 개선되었다. Total cholesterol, LDL cholesterol, and triglycerides exceeded the normal range at the start of the clinical trial, but there was no change in the control group for 2 months after ingesting the polymer silk peptide, whereas these levels gradually decreased in the experimental group. Total cholesterol, LDL cholesterol, and triglycerides were all improved to near normal levels. HDL cholesterol was in the normal range from the beginning, and remained in the normal range after 2 months, and the ratio of HDL cholesterol to total cholesterol improved from 23.5% to 25.1%.
이들 결과로부터 고분자 실크 펩타이드는 총콜레스테롤, LDL 콜레스테롤, 중성지방을 개선하는 효과가 뛰어나다는 것을 확인할 수 있었다.
From these results, it was confirmed that the polymer silk peptide is excellent in improving total cholesterol, LDL cholesterol, and triglyceride.
이상 살펴본 바와 같이, 본 발명에 의하여 제조된 고분자의 실크 펩타이드를 섭취함으로써, 그 결과로 고콜레스테롤증, 고지혈증 개선의 복합기능을 가져 이들 질환의 치료 및 예방을 위하여 사용될 수 있다. As described above, by ingesting the silk peptide of the polymer prepared according to the present invention, as a result, it has a combined function of improving hypercholesterolemia, hyperlipidemia can be used for the treatment and prevention of these diseases.
Claims (10)
Functional food composition for improving blood lipid-related diseases produced by decomposition of silk protein, comprising a silk peptide having a weight average molecular weight of 10,000 to 100,000 as an active ingredient
The composition according to claim 1, wherein the improvement in blood lipid-related diseases is improvement in blood total cholesterol, improvement in blood LDL cholesterol, or improvement in blood triglycerides.
The composition of claim 1, wherein the silk protein is silk fibroin.
The method of claim 1, wherein the decomposition of the silk protein is characterized in that it is carried out by a decomposition method selected from the group consisting of decomposition in calcium salt solution, acid hydrolysis, decomposition by a mixture solution of calcium salt and ethanol, and combinations thereof. Composition for improving diabetes
The composition of claim 1 wherein the weight average molecular weight of the silk peptide is 20,000-50,000.
The composition according to claim 5, wherein the weight average molecular weight of the silk peptide is more preferably 25,000 to 30,000.
The method of claim 4, wherein the degradation of the silk protein comprises: (a) dissolving silk fibroin in a solution containing calcium salt; (b) removing the calcium salt contained in the silk fibroin solution
The composition according to claim 4, wherein the calcium salt is calcium chloride.
The composition of claim 7 wherein the removal of calcium salt is selected from a method consisting of dialysis, ultrafiltration, gel chromatography, electrodesulfation and combinations thereof.
The composition according to claim 7, wherein the removal of calcium salt is more preferably a method consisting of ultrafiltration.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| WO2015048805A1 (en) | 2013-09-30 | 2015-04-02 | Silk Therapeutics, Inc. | Silk potein fragment compositions and articles manufactured therefrom |
| EP3052203A4 (en) * | 2013-09-30 | 2017-08-23 | Silk Therapeutics Inc. | Silk protein fragment compositions and articles manufactured therefrom |
| US10987294B2 (en) | 2013-09-30 | 2021-04-27 | Evolved By Nature, Inc. | Stable silk fibroin based pharmaceutical formulations |
| US11298311B2 (en) | 2013-09-30 | 2022-04-12 | Evolved By Nature, Inc. | Stable silk protein fragment compositions |
| US11298310B2 (en) | 2013-09-30 | 2022-04-12 | Evolved By Nature, Inc. | Stable silk protein fragment compositions |
| US11857663B2 (en) | 2013-09-30 | 2024-01-02 | Evolved By Nature, Inc. | Stable silk protein fragment compositions |
| US11857664B2 (en) | 2013-09-30 | 2024-01-02 | Evolved By Nature, Inc. | Stable silk protein fragment compositions |
| EP4509520A3 (en) * | 2013-09-30 | 2025-10-22 | Evolved by Nature, Inc. | Silk protein fragment compositions and articles manufactured therefrom |
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